• The Plant Pathology Journal
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• 제30권1호
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• pp.58-67
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• 2014

The multifunctional triple gene block protein 1 (TGB1) of the Potexvirus Alternanthera mosaic virus (AltMV) has been reported to have silencing suppressor, cell-to-cell movement, and helicase functions. Yeast two hybrid screening using an Arabidopsis thaliana cDNA library with TGB1 as bait, and co-purification with TGB1 inclusion bodies identified several host proteins which interact with AltMV TGB1. Host protein interactions with TGB1 were confirmed by biomolecular fluorescence complementation, which showed positive TGB1 interaction with mitochondrial ATP synthase delta' chain subunit (ATP synthase delta'), light harvesting chlorophyll-protein complex I subunit A4 (LHCA4), chlorophyll a/b binding protein 1 (LHB1B2), chloroplast-localized IscA-like protein (ATCPISCA), and chloroplast ${\beta}$-ATPase. However, chloroplast ${\beta}$-ATPase interacts only with $TGB1_{L88}$, and not with weak silencing suppressor $TGB1_{L88}$. This selective interaction indicates that chloroplast ${\beta}$-ATPase is not required for AltMV movement and replication; however, TRV silencing of chloroplast ${\beta}$-ATPase in Nicotiana benthamiana induced severe tissue necrosis when plants were infected by AltMV $TGB1_{L88}$ but not AltMV $TGB1_{L88}$, suggesting that ${\beta}$-ATPase selectively responded to $TGB1_{L88}$ to induce defense responses.

• The Plant Pathology Journal
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• 제29권4호
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• pp.454-459
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• 2013

The Potexvirus Alternanthera mosaic virus (AltMV) has multifunctional triple gene block (TGB) proteins, among which our studies have focused on the properties of the TGB1 protein. The TGB1 of AltMV has functions including RNA binding, RNA silencing suppression, and cell-to-cell movement, and is known to form homologous interactions. The helicase domains of AltMV TGB1 were separately mutated to identify which regions are involved in homologous TGB1 interactions. The yeast two hybrid system and Bimolecular Fluorescence Complementation (BiFC) in planta were utilized to examine homologous interactions of the mutants. Helicase motif I of AltMV TGB1 was found to be critical to maintain homologous interactions. Mutations in the remaining helicase motifs did not inhibit TGB1 homologous interactions. In the absence of homologous interaction of TGB1, subcellular localization of helicase domain I mutants showed distinctively different patterns from that of WT TGB1. These results provide important information to study viral movement and replication of AltMV.

• The Plant Pathology Journal
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• 제29권1호
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• pp.17-30
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• 2013

Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin filaments, and that TGB1 alone had no obvious effects on actin filament structure. Latrunculin B (LatB) treat-ments retarded BSMV cell-to-cell movement, disrupted actin filament organization, and dramatically decreased the proportion of paired TGB3 foci appearing at the cell wall (CW). BSMV infection of transgenic plants tagged with GFP-KDEL exhibited membrane proliferation and vesicle formation that were especially evident around the nucleus. Similar membrane proliferation occurred in plants expressing TGB2 and/or TGB3, and DsRed: Talin fluorescence in these plants colocalized with the ER vesicles. TGB3 also associated with the Golgi apparatus and overlapped with cortical vesicles appearing at the cell periphery. Brefeldin A treatments disrupted Golgi and also altered vesicles at the CW, but failed to interfere with TGB CW localization. Our results indicate that actin cytoskeleton interactions are important in BSMV cell-to-cell movement and for CW localization of TGB3.

• Journal of Animal Science and Technology
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• 제54권4호
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• pp.307-313
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• 2012

본 연구는 glucomannan, carrageenan, carboxymethyl cellulose 및 transglutaminase-B의 0.5% 첨가가 돈육껍질 돈육껍질결체조직 함유 돈육 패티의 품질특성에 미치는 영향에 대해 알아보고자 실시하였다. 육색의 경우, 대조구와 처리구들간 유의적인 차이가 나타나지 않았다. 관능평가 또한 모든 처리구들간 유의적인 차이가 나타나지 않았다(P>0.05). 그러나 가열감량의 경우, 저장말기인 21일에 대조구에 비해 모든 처리구들에서 낮은 가열감량 값을 나타내며, 전단가 또한 저장 14일에 CMC 및 TGB 처리구들에서, 저장 21일차엔 GMN 및 TGB 처리구에서 대조구에 비해 낮은 전단가 값을 나타내었다(P<0.05). 지방산패도 결과, 저장 1일에 CAR 처리구에서 가장 높은 지방산패도 값을 보였으나, GMN, CMC 및 TGB 처리구에서 대조구에 비해 낮은 값을 나타내었다. 이러한 경향은 저장말기인 21일에 GMN 및 TGB 처리구에서 낮은 지방산패도 값으로 나타났다(P<0.05). 또한 저장 21일의 모든 처리구에서 대조구보다 낮은 휘발성염기태질소 값을 나타내었다(P<0.05). 이상의 결과를 종합하면, 돈육껍질 함유 돈육 패티에 GMN, CAR, CMC 및 TGB의 첨가는 육색과 관능평가에 영향을 미치진 못하지만, 패티의 가열감량을 줄여 연한 조직감을 형성할 것으로 판단된다. 또한 GMN 및 TGB 첨가는 저장 중 패티의 지방산패도를 억제시키며, 저장말기 휘발성염기태질소 값을 떨어뜨려 저장성 향상에 기여 할 것으로 판단된다.

• The Plant Pathology Journal
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• 제37권2호
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• pp.152-161
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• 2021

Sugar beet (Beta vulgaris L.) is known as a key product for agriculture in several countries across the world. Beet soil-borne virus (BSBV) triggers substantial economic damages to sugar beet by reducing the quantity of the yield and quality of the beet sugars. We conducted the present study to report the complete genome sequences of two BSBV isolates in Turkey for the first time. The genome organization was identical to those previously established BSBV isolates. The tripartite genome of BSBV-TR1 and -TR3 comprised a 5,835-nucleotide (nt) RNA1, a 3,454-nt RNA2, and a 3,005-nt RNA3 segment. According to sequence identity analyses, Turkish isolates were most closely related to the BSBV isolate reported from Iran (97.83-98.77% nt identity). The BSBV isolates worldwide (n = 9) were phylogenetically classified into five (RNA-coat protein read through gene [CPRT], TGB1, and TGB2 segments), four (RNA-rep), or three (TGB3) lineages. In genetic analysis, the TGB3 revealed more genetic variability (Pi = 0.034) compared with other regions. Population selection analysis revealed that most of the codons were generally under negative selection or neutral evolution in the BSBV isolates studied. However, positive selection was detected at codon 135 in the TGB1, which could be an adaptation in order to facilitate the movement and overcome the host plant resistance genes. We expect that the information on genome properties and genetic variability of BSBV, particularly in TGB3, TGB1, and CPRT genes, assist in developing effective control measures in order to prevent severe losses and make amendments in management strategies.

• 농업과학연구
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• 제44권2호
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• pp.145-180
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• 2017

Alternanthera mosaic virus (AltMV) is a member of the genus Potexvirus which has been known for less than twenty years, and has been detected in Australasia, Europe, North and South America, and Asia. The natural host range to date includes species in at least twenty-four taxonomically diverse plant families, with species in at least four other families known to be infected experimentally. AltMV has been shown to differ from Potato virus X (PVX), the type member of the genus Potexvirus, in a number of ways, including the subcellular localization of the Triple Gene Block 3 (TGB3) protein and apparent absence of interactions between TGB3 and TGB2. Differences between AltMV variants have allowed identification of viral determinants of pathogenicity, and identification of residues involved in interactions with host proteins. Infectious clones of AltMV differing significantly in symptom severity and efficiency of RNA silencing suppression have been produced, suitable either for high level protein expression (with efficient RNA silencing suppression) or for Virus-Induced Gene Silencing (VIGS; with weaker RNA silencing suppression), demonstrating a range of utility not available with most other plant viral vectors. The difference in silencing suppression efficiency was shown to be due to a single amino acid residue substitution in TGB1, and to differences in subcellular localization of TGB1 to the nucleus and nucleolus. The current state of knowledge of AltMV biology, including host range, strain differentiation, host interactions, and utility as a plant viral vector for both protein expression and VIGS are summarized.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.113.1-113
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• 2003

A potexvirus, Hosta virus X (HVX-Kr), causing mosaic and mottle symptoms was isolated from hosta plants (Hosta spp.), and its entire genome RNA sequence was determined. in Korea using cDNA library and RACE methods. The genome of HVX encodes five open reading frames coding for viral replicase, triple gene block (TGB), and viral coat protein (CP) from the 5'to 3' ends, which is a typical genome structure of potexviruses. The 3-terminal region of the virus includes the TGBI (26 kDa), TGB2 (13 kDa), TGB3 (8 kDa), and 23 kDa coat protein (CP) and the 3-nontranslated region (NTR). The CP gene of the type isolate of HVX (HVX-U) was amplified by RT-PCR and its nucleotide sequence was determined. The CPs of HVX-Kr and HVX-U had 100％ and 98.9％ identical amino acids and nucleotides, respectively. Most of the regions of the genome HVX had over 50％ nucleotide identical to other sequenced potexviruses. This is the first report of complete genome sequence information of HVX and molecular evidence supporting the virus as a distinct species of the genus Potexvirus.

• 항공우주기술
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• 제4권2호
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• pp.50-59
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• 2005

본 연구에서는 개발된 테일팬 1차 시제품의 성능시험에 대한 것이다. 한국항공우주연구원에서는 헬리콥터용 반 토오크 시스템으로 테일팬 시스템을 개발하였으며, 제작된 1차 시제품의 성능시험을 위해 테일팬 성능시험장치를 제작하였다. 테일팬 성능시험을 위해 먼저 시험장치에 대한 제작 검증용 시험을 수행하였고, 다음으로 팬블레이드와 시험장치의 고유진동수를 확인하였다. 성능 시험용 시험장치 운용회전수를 결정하기 위한 운용시험을 수행하였다. 테일기어박스(TGB)에 가속도계를 부착하여 단계적으로 회전수와 부착물들을 변화시켜가며 고유진동수와 진동 레벨을 확인하였다. 운용시험 결과로부터 테일팬 성능시험용 시험장치 운용회전수를 결정하였고, 무풍조건과 비행 조건에 따른 성능시험을 수행하였다. 측정된 데이터는 무차원화하여 분석하였고, 이 결과로 개발 시제품이 성능 요구조건을 만족시킴을 확인할 수 있었다.

• 전자통신동향분석
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• 제25권1호
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• pp.114-123
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• 2010

멀티모드 휴대 단말의 보급이 본격화되고 다양한 유무선 통신 인프라가 확대됨에 따라 이기종 네트워크 간의 핸드오버 서비스가 무엇보다 요구되는 시점이다. IEEE 802.21 WG에서는 이기종 네트워크간 심리스한 수직 핸드오버 서비스를 목적으로 MIH 표준 기술을 발간하였고, 최근에는 MIH 기술을 방송 네트워크로의 핸드오버를 위해 사용하도록 추가적인 표준화 작업을 진행하고 있다. 본 글은 이기종 네트워크간의 수직 핸드오버 서비스에 효과적으로 사용될 수 있는 IEEE 802.21 MIH 표준의 주요 핵심 기술 및 표준화 현황에 대해 알아보고 통신 네트워크와 방송 네트워크 간의 핸드오버 서비스 제공을 위해 최근 IEEE 802.21 WG 산하에 생성된 IEEE 802.21 TGb의 표준화 진행사항에 대하여 정리해 본다.

• 생명과학회지
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• 제11권2호
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• pp.181-189
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• 2001

Cytokines are hormone-like proteins which mediate and regulast inflammatory and immune responses. Transforming growth factor -$\beta$1(TGF-$\beta$) plays an important role in the control of the immune response and wound healing, and in the development o various tissues and organs, Nitric oxide(NO) is major messenger molecule regulating immune function and blood vessel dilation and serving as a neurotransmitter in the brain and peripheral nervous system. Also, NO is to be a potent mutagen that cause mutation in the p53 tumor suppressor gene in early phases of human gastric carcinogenesis. The purpose of this study was to investigate the effect of Helicobacter phlori lystes, lipopolysaccharide (LPS), and Staphylococcus enterotoxin B(SEB) on production of TGF-$\beta$1 and NO by human fibroblasts. Primary cultured human fibroblasts were incubated with H. pylori lysates(Hp), LPs, SEB, Hp+LPS, Hp+SEB, Hp+LPS+SEB. Cultured supernatants that were collected at 24, 48 and 72 hr were assessed for TGF-$\beta$1 by enzyme-linked immunosorbent assay and NO production by quantification of nitrite ion. TGF-$\beta$1 production in fibroblasts exposed with Hp, LPS or SEB for 48 hrs was enhanced, but for 72 hrs inhibited. Its production by doble exposure such as Hp+LPS, Hp+SEB, Hp+LPS+SEB was lowered in comparison with single exposure of Hp in cases of 24 and 48 hrs incubation, but for 72 hrs decreased in Hp vaculoating toxin(+), increased in Hp vacuolating toxin(-). No production in fibroblasts increaed at all doses of LPS. But its production by exposure of SEB increased or decreased according to dose and incubation time. Also, NO production by Hp vacuolating toxin(+) increased at all doses, but its production by Hp vacuolating toxin(-) decreased. Its production by doble exposure such as Hp+LPS, Hp+SEB, Hp+LPS+SEB decreased in comparison with single exposure Hp Therefore, quantities pf TGB-$\beta$1 and NO released by human fibroblasts shows differences according to kinds of stimulants. Also, in care stimulated with same kinds of stimulants, its productions exhibit quantitative differences according to exposure times. These results suggest that the decreased of TGF-$\beta$1 in fibroblasts by mixed exposure with Hp producing vacuolating toxin and bacterial toxins such as LPS and SEB may effect negatively in healing of host tissue and increased of NO by infection oh H. pylori may related to the increased susceptibility for human gastric carcinogenesis.