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http://dx.doi.org/10.5423/PPJ.NT.04.2013.0045

AltMV TGB1 Nucleolar Localization Requires Homologous Interaction and Correlates with Cell Wall Localization Associated with Cell-to-Cell Movement  

Nam, Jiryun (Department of Applied Biology, Chungnam National University)
Nam, Moon (Department of Applied Biology, Chungnam National University)
Bae, Hanhong (Department of Biological Science, Youngnam University)
Lee, Cheolho (Department of Chemical and Biological Engineering, Seokyoung University)
Lee, Bong-Chun (Crop Environment Research Division, National Institute of Crop Science, RDA)
Hammond, John (USDA-ARS, US National Arboretum, Floral and Nursery Plants Research Unit)
Lim, Hyoun-Sub (Department of Applied Biology, Chungnam National University)
Publication Information
The Plant Pathology Journal / v.29, no.4, 2013 , pp. 454-459 More about this Journal
Abstract
The Potexvirus Alternanthera mosaic virus (AltMV) has multifunctional triple gene block (TGB) proteins, among which our studies have focused on the properties of the TGB1 protein. The TGB1 of AltMV has functions including RNA binding, RNA silencing suppression, and cell-to-cell movement, and is known to form homologous interactions. The helicase domains of AltMV TGB1 were separately mutated to identify which regions are involved in homologous TGB1 interactions. The yeast two hybrid system and Bimolecular Fluorescence Complementation (BiFC) in planta were utilized to examine homologous interactions of the mutants. Helicase motif I of AltMV TGB1 was found to be critical to maintain homologous interactions. Mutations in the remaining helicase motifs did not inhibit TGB1 homologous interactions. In the absence of homologous interaction of TGB1, subcellular localization of helicase domain I mutants showed distinctively different patterns from that of WT TGB1. These results provide important information to study viral movement and replication of AltMV.
Keywords
AltMV; homologous interaction; subcellular localization; TGB1;
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1 Lim, H. S., Vaira, A. M., Reinsel, M. D., Bae, H., Bailey, B. A., Domier, L. L. and Hammond, J. 2010c. Pathogenicity of Alternanthera mosaic virus is affected by determinants in RNA-dependent RNA polymerase and by reduced efficacy of silencing suppression in a movement-competent TGB1. J. Gen. Virol. 91:277-287.   DOI   ScienceOn
2 Lin, M. K., Chang, B. Y., Liao, J. T., Lin, N. S. and Hsu, Y. H. 2004. Arg-16 and Arg-21 in the N-terminal region of the triple-gene-block protein 1 of Bamboo mosaic virus are essential for virus movement. J. Gen. Virol. 85:251-259.   DOI   ScienceOn
3 Lin, N. S. and Chen, C. C. 1991. Association of bamboo mosaic virus (BoMV) and BoMV-specific electron-dense crystalline bodies with chloroplasts. Phytopathology 81:1551-1555.   DOI
4 Liou, D. Y., Hsu, Y. H., Wung, C. H., Wang, W. H., Lin, N. S. and Chang, B. Y. 2000. Functional analyses and identification of two arginine residues essential to the ATP-utilizing activity of the triple gene block protein 1 of bamboo mosaic Potexvirus. Virology 277:336-344.   DOI   ScienceOn
5 Morozov, S. Y. and Solovyev, A. G. 2003. Triple gene block: modular design of a multifunctional machine for plant virus movement. J. Gen. Virol. 84:1351-1366.   DOI   ScienceOn
6 Rouleau, M., Smith, R. J., Bancroft, J. B. and Mackie, G. A. 1994. Purification, properties, and subcellular localization of Foxtail mosaic potexvirus 26-kDa protein. Virology 204:254-265.   DOI   ScienceOn
7 Shiboleth, Y. M., Haronsky, E., Leibman, D., Arazi, T., Wassenegger, M., Whitham, S. A., Gaba, V. and Gal-On, A. 2007. The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development. J. Virol. 81:13135-13148.   DOI   ScienceOn
8 Silhavy, D. and Burgyan, J. 2004. Effects and side-effects of viral RNA silencing suppressors on short RNAs. Trends Plant Sci. 9:76-83.
9 Verchot-Lubicz, J., Torrance, L., Solovyev, A. G., Morozov, S. Y., Jackson, A. O. and Gilmer, D. 2010. Varied movement strategies employed by triple gene block-encoding viruses. Mol. Plant-Microbe Interact. 23:1231-1247.   DOI   ScienceOn
10 Verchot-Lubicz, J., Ye, C. M. and Bamunusinghe, D. 2007. Molecular biology of Potexviruses: recent advances. J. Gen. Virol. 88:1643-1655.   DOI   ScienceOn
11 Voinnet, O., Lederer, C. and Baulcombe, D. C. 2000. A viral movement protein prevents spread of the gene silencing signal in Nicotiana benthamiana. Cell 103:157-167.   DOI   ScienceOn
12 Waadt, R., Schmidt, L. K., Lohse, M., Hashimoto, K., Bock, R. and Kudla, J. 2008. Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta. Plant J. 56:505-516.   DOI   ScienceOn
13 Wung, C. H., Hsu, Y. H., Liou, D. Y., Huang, W. C., Lin, N. S. and Chang, B. Y. 1999. Identification of the RNA-binding sites of the triple gene block protein 1 of bamboo mosaic Potexvirus. J. Gen. Virol. 80:1119-1126.   DOI
14 Bragg, J. N. and Jackson, A. O. 2004. The C-terminal region of the Barley stripe mosaic virus b protein participates in homologous interactions and is required for suppression of RNA silencing. Mol. Plant Pathol. 5:465-481.   DOI   ScienceOn
15 Abdel-Ghany, S. E., Ye, H., Garifullina, G. F., Zhang, L., Pilon-Smits, E. A. and Pilon, M. 2005. Iron-sulfur cluster biogenesis in chloroplasts. Involvement of the scaffold protein CpIscA. Plant Physiol. 138:161-172.   DOI   ScienceOn
16 Bayne, E. H., Rakitina, D. V., Morozov, S. Y. and Baulcombe, D. C. 2005. Cell-to-cell movement of potato potexvirus X is dependent on suppression of RNA silencing. Plant J. 44:471-482.   DOI   ScienceOn
17 Beck, D. L., Guilford, P. J., Voot, D. M., Andersen, M. T. and Forster, R. L. 1991. Triple gene block proteins of white clover mosaic potexvirus are required for transport. Virology 183:695-702.   DOI   ScienceOn
18 Canetta, E., Kim, S. H., Kalinina, N. O., Shaw, J., Adya, A. K., Gillespie, T., Brown, J. W. and Taliansky, M. 2008. A plant virus movement protein forms ringlike complexes with the major nucleolar protein, fibrillarin, in vitro. J. Mol. Biol. 376:932-937.   DOI   ScienceOn
19 Davies, C., Hills, G. and Baulcombe, D. C. 1993. Sub-cellular localization of the 25-kDa protein encoded in the triple gene block of potato virus X. Virology 197:166-175.   DOI   ScienceOn
20 Hammond, J., Reinsel, M. D. and Maroon-Lango, C. J. 2006. Identification and full sequence of an isolate of Alternanthera mosaic Potexvirus infecting Phlox stolonifera. Arch. Virol. 151:477-493.   DOI
21 Lim, H. S., Bragg, J. N., Ganesan, U., Lawrence, D. M., Yu, J., Isogai, M., Hammond, J. and Jackson, A. O. 2008. Triple gene block protein interactions involved in movement of Barley stripe mosaic virus. J. Virol. 82:4991-5006.   DOI   ScienceOn
22 James, P., Halladay, J. and Craig, E. A. 1996. Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics 144:1425-1436.
23 Kim, S. H., MacFarlane, S., Kalinina, N. O., Rakitina, D. V., Ryabov, E. V., Gillespie, T., Haupt, S., Brown, J. W. and Taliansky, M. 2007. Interaction of a plant virus-encoded protein with the major nucleolar protein fibrillarin is required for systemic virus infection. Proc. Natl. Acad. Sci. U.S.A. 104:11115-11120.   DOI   ScienceOn
24 Leshchiner, A. D., Solovyev, A. G., Morozov, S. Y. and Kalinina, N. O. 2006. A minimal region in the NTPase/helicase domain of the TGBp1 plant virus movement protein is responsible for ATPase activity and cooperative RNA binding. J. Gen. Virol. 87:3087-3095.   DOI   ScienceOn
25 Lim, H. S., Bragg, J. N., Ganesan, U., Ruzin, S., Schichnes, D., Lee, M. Y., Vaira, A. M., Ryu, K. H., Hammond, J. and Jackson, A. O. 2009. Subcellular localization of the barley stripe mosaic virus triple gene block proteins. J. Virol. 83:9432-9448.   DOI   ScienceOn
26 Lim, H. S., Vaira, A. M., Bae, H., Bragg, J. N., Ruzin, S. E., Bauchan, G. R., Dienelt, M. M., Owens, R. A. and Hammond, J. 2010a. Mutation of a chloroplast-targeting signal in Alternanthera mosaic virus TGB3 impairs cell-to-cell movement and eliminates long-distance virus movement. J. Gen. Virol. 91:2102-2115.   DOI   ScienceOn
27 Lim, H. S., Vaira, A. M., Domier, L. L., Lee, S. C., Kim, H. G. and Hammond J. 2010b. Efficiency of VIGS and gene expression in a novel bipartite Potexvirus vector delivery system as a function of strength of TGB1 silencing suppression. Virology 402:149-163.   DOI   ScienceOn