• 생명과학회지
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• 제19권9호
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• pp.1289-1293
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• 2009

원암단백질로 알려진 Human cervical cancer oncogene (HCCR)은 발암억제 단백질인 p53과 작용하여 다양한 암조직에서 암의 유발을 촉진한다. 그러나, 아직 정확한 발암 유도기전이 알려져 있지 않다. 이러한 의문을 해소하기 위한 일환으로 본 연구에서는 HCCR의 발현이 어떻게 조절되는지를 조사하였다. 이를 위해 먼저 HCCR 5' 쪽의 promoter 영역을 분리하여 Luciferase assay법을 이용하여 K562, HEK293, A549 세포에서 분석하였고, Promoter의 -370에서 -406사이 36bp의 첨가로 promoter활성이 의미 있게 억제됨을 관찰하였다. 또한, 36 bp만을 포함하는 probe를 이용한 mobility shift assays (EMSA)에서 핵단백질이 결합함을 관찰하였고, 컴퓨터를 이용한 분석에서 TG-interacting factor (TGIF)에 대한 consensus sequences 존재함을 관찰하였다. TGIF 만을 포함하는 probe (TC)와 돌연변이를 유발한 probe (mTG)를 이용한 EMSA에서 이 자리에 TGIF가 결합함을 보였다. 또한, TGIF 자리에 돌연변이를 유발하면(pGL3-mTGIF) 발현의 억제가 회복됨을 관찰하였다. 본 연구에서는 HCCR promoter의 특성을 분석하였고, 이 과정에서 -390에서 -366 사이에 TGIF 전사인자가 결합하여 전사활성을 조절함을 증명하였다.

• 한국산학기술학회논문지
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• 제12권7호
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• pp.3072-3081
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• 2011

본 연구는 일부 종합검진 수검자들을 대상으로 BMI와 혈압, 혈액학적 및 혈액생화학적 지표와의 관련성을 검토하고자 2007년 1월부터 2009년 12월까지의 기간에 한국건강관리협회 대전지부 건강검진센터에서 종합건강검진을 받았던 20세 이상의 지역주민 3,731명(남자 2,312명, 여자 1,419명)을 분석대상으로 하였다. 연구 결과 남녀 모두 BMI가 증가할수록 SBP, DBP, TG, TC, LDL-C, AST, GGT, ALP의 평균치는 유의하게 증가하였으며, HDL-C는 유의하게 감소하였다. BMI는 SBP, DBP, TG, TC, LDL-C 및 GGT와 유의한 양의 상관관계가 인정되었고, HDL-C와는 유의한 음의 상관관계가 인정되었다. 순서형 다변량 로지스틱 회귀분석 결과 BMI가 증가함에 따라 SBP, DBP, TG, ALT, GGT의 비정상치에 대한 위험비가 유의하게 증가하였다.

• Journal of Forest and Environmental Science
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• 제32권4호
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• pp.323-328
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• 2016

Thermal stability and rheological behaviors of Ricinodendron heudelotii wood were investigated. Thermogravimetric analysis conducted at a heating rate of $10^{\circ}C/min$ from 20 to $600^{\circ}C$ in a nitrogen atmosphere indicated that there was no variation in the decomposition of the onset and final temperature for all the polymers. The thermal behaviours were investigated at a temperature range from 130 to $0^{\circ}C$ at $3^{\circ}C/min$, multi-frequencies of 0.1-10 Hz using dynamic mechanical analysis. N-methyl-2-pyrolidone saturated specimens were tested while submerged under the same solvent. Polymers decomposition pattern during thermogravimetric analysis are similar in the radial position of the wood. The glass transition temperature (Tg) of R. heudelotii is $45{\pm}1^{\circ}C$ at 0.1 Hz. The Tg differs from the innerwood to outerwood. The Tg showed that N-methyl-2-pyrolidone saturated R. heudelotii would require low energy consumption during chemi-thermomechanical pulping.

• Elastomers and Composites
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• 제51권3호
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• pp.206-211
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• 2016

Experimental error sources for thermogravimetric analysis (TGA) of poly(ethylene-co-vinyl acetate) (EVA) were investigated and sample preparation method to reduce the experimental error was suggested. Maximum dissociation temperatures of EVA for the first and second dissociation reactions ($T_{m1}$ and $T_{m2}$, respectively) were measured. By decreasing the weight of raw EVA, the $T_{m1}$ increased but the the $T_{m2}$ decreased. When weight of the raw EVA was over 10 mg, the TGA curve showed abnormal behaviors. The abnormal TG behaviors were explained by gathering and instantaneous evaporation of acetic acid formed by deacetylation of the VA unit. When TGA analysis of EVA was performed using untreated (raw) EVA, the experimental errors were about 1%. In order to eliminate the abnormal TG behaviors and to reduce the experimental errors, EVA film made by solvent casting was used. For the treated EVA (EVA film), the abnormal TG behaviors did not appear, the $T_{m1}$ decreased by about $2^{\circ}C$ but the $T_{m2}$ increased by about $6^{\circ}C$, and the experimental errors were reduced by 0.5%.

• 한국콘텐츠학회논문지
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• 제20권11호
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• pp.525-533
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• 2020

본 연구는 10주간의 복합운동프로그램 참여가 산후비만여성들의 대사증후군관련인자(HDL-C, TG, Glucose, 혈압, 허리둘레) 및 아디포사이토카인(Leptin, Adiponectin)에 어떠한 영향을 미치는지 알아보았다. 연구대상자는 출산 후 40개월 이내 여성 총 14명으로 복합운동군 7명, 통제군 7명으로 배정하였다. 복합 운동 프로그램은 리듬운동을 적용한 유산소운동과 근력운동을 실시하였으며 주 2회, 80분간, 10주간 실시하였다. 자료처리를 위해 Mann Whitney U-test, Wilcoxon test, Spearman 상관분석을 실시하였다. 연구 결과, 대사증후군 관련인자에서 운동 실시 전, 후모든 항목에서 그룹 간 차이가 나타나지 않았다. 그룹 내 차이에서는 복합운동군에서 HDL-C 유의한 증가를 보였으며 TG, Glucose, 허리둘레에서는 유의한 감소를 나타냈고 통제군에서는 TG, Glucose에서 유의한 증가를 나타냈다. 아디포사이토카인의 결과를 보면, 그룹 내 차이에서만 복합운동군이 Adiponectin에서 운동 후 유의한 증가를 나타나냈다. 대사증후군 관련인자와 아디포사이토카인과의 상관을 보면 HDL-C, Glucose가 Adiponectin과 상관이 있는 것으로 나타났다.

• 한국식품조리과학회지
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• 제29권5호
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• pp.543-552
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• 2013

This study investigated the free sugar and free amino acids considered as the taste component in Korean Gochujang. Our goal was to search and develop the Korean traditional Gochujang taste, and to monitor the current status and characteristics of the Gochujang. For the analysis of Gochujang, it is purchased from small farms as well as major food company producing Gochujang. In the case of commercial Gochujang(COM), glucose and maltose were major free sugar, and the ratio of distribution and total amount showed very similar pattern. However, the results of the traditional Gochujang(TG) showed significant differences for each sample even they had glucose and maltose as predominant sugar. The content of glucose, maltose, fructose was reduced in order. The other hand, sucrose, rhamnose were not detected or were detected trace amounts in some samples. Even the characteristics were found at each region, it was no noticeable difference, but each sample was greater variation. Total of 17 amino acids were found from COM and the major amino acids were Pro, Glu, Asp, but His, Met were generally detected in small amounts. In the TG, they mainly contained Glu, Asp, Pro as the dominant component in addition to the Arg, Ala, Cys, respectively. TG had higher amino acid content and fairly various distribution compared to COM. It could suggest the possibility of the development for different traditional tastes because each TG had diverse characteristic taste than COM.

• 대한정형도수물리치료학회지
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• 제22권1호
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• pp.9-15
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• 2016

Background: This study was conducted to investigate the effects of unstable modified wall squat exercises accompanied by abdominal drawing-in on the gait variables of healthy adults. Methods: The total number of subjects was 30, and 15 were randomly placed in the training group (TG) and 15 in the control group (CG). To determine the gait variables of TG and CG, step length difference (SLD) stance phase difference (STPD), swing phase difference (SWPD), single support difference (SSD), and step time difference (STD) were measured using OptoGait, a gait analysis system. Results: When the pre-intervention and post-intervention results of TG and CG were compared, statistically significant differences in SLD, STPD, SWPD, SSD and STD of TG were seen. Conclusion: Unstable modified wall squat exercises accompanied by abdominal drawing-in might help reduce the deviation between left and right gait variables during walking.

• Clinical and Experimental Reproductive Medicine
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• 제44권1호
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• pp.28-32
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• 2017

Objective: Growing evidence suggests that increased cardiovascular disease (CVD) risk is associated with female infertility caused by conditions such as polycystic ovarian disease, obesity, thyroid dysfunction, and endometriosis. The aim of this study was to evaluate whether any relationship exists between CVD and unexplained infertility. Methods: Sixty-five women with unexplained infertility and 65 fertile controls were enrolled in the study. CVD risk markers such as low-density lipoprotein (LDL), high-density lipoprotein (HDL), total cholesterol (TC), triglycerides (TG), insulin resistance (defined by the homeostasis model assessment ratio), and high-sensitivity C-reactive protein (hs-CRP) were assessed. Results: TG, TC, LDL, and hs-CRP levels were higher and HDL levels were lower in patients with unexplained infertility than in fertile controls (p<0.05 for all). Positive associations were found between unexplained infertility and TG, TC, LDL, and hs-CRP levels, and a negative correlation was found for HDL (p<0.05 for all). Multivariate logistic regression analysis showed that TG, HDL, and hs-CRP were independent variables associated with unexplained infertility. Conclusion: Our study showed that women with unexplained infertility had an atherogenic lipid profile and elevated hs-CRP levels, suggesting a higher risk of developing CVD in the future. Further studies with larger groups are needed to investigate the nature of this link.

• 대한의생명과학회지
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• 제17권2호
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• pp.123-128
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• 2011

We analyzed the concentration of serum total cholesterol (TC), high density lipoprotein cholesterol (HDLC), low density lipoprotein cholesterol (LDLC), triglyceride (TG) and the serum lipoprotein electrophoresis fractions in hypertensive patients (hypertension group, n=182). The average concentration of lipids and lipoprotein fractions in the hypertension group was compared to that of the normal group. The average concentrations of serum TC, LDLC and TG in the hypertension group were significantly higher than those of the normal group (3.3%, 11% and 70%, respectively) (P<0.05). But HDLC was nonsignificantly lower (2%) (P<0.05). In the hypertension group, the percentages of patients who had an abnormally high level of total cholesterol, HDLC, LDLC and TG were 12.1%, 8.2%, 24.2% and 44.5%, respectively. The average of ${\beta}$-lipoprotein (27.5%) and pre ${\beta}$-lipoprotein (12.6%) were significantly higher, whereas ${\alpha}$-lipoprotein (15.2%) was significantly lower in the hypertension group than in the normal group (P<0.05). The percentages of patients who had an abnormally high level of very low density lipoprotein (pre ${\beta}$-LP), LDLC (${\beta}$-LP) and chylomicron were 31.3%, 17.0% and 12.6%, respectively. This study suggests that an increasing of total cholesterol, LDLC and TG, pre ${\beta}$-LP, ${\beta}$-LP and chylomicron are associated with a risk factor for hypertension.

• BMB Reports
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• 제40권1호
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• pp.36-43
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• 2007

In this study, cutaneous role of IL-4 in UVB-induced apoptosis was investigated using transgenic mice with skin-specific expression of IL-4 (IL-4 Tg mice). The transgenic mice did not show any gross clinical abnormalities. However, epidermis was thickened and increased MHC class II positive cells were detected as well as enhanced expression of inflammatory cytokines such as IL-1 and TNF-$\alpha$ in skin. In addition, histological analysis revealed increased infiltration of lymphocytes, acanthosis, hyperkeratosis, and parakeratosis in skin of IL-4 Tg mice. The physiological effect of IL-4 overexpression in skin against environmental stimulus such as UVB was investigated by irradiating wild-type and IL-4 Tg mice with UVB followed by evaluation of apoptosis. The result demonstrated suppressed apoptosis in epidermis of IL-4 Tg mice compared with wild-type mice. To further assess anti-apoptotic function of IL-4 in keratinocytes, stable cell clones were made where IL-4 was constitutively overexpressed and examined for UVB-induced apoptosis. The results showed that apoptosis was remarkably decreased in IL-4 over-expressing cell clones compared with that in mock transfected cells. Collectively, data presented here shows that IL-4 has an inhibitory effect against UVB-induced apoptosis in keratinocytes, suggesting that IL-4 may be an important regulator in cutaneous immunity against UVB.