• Title/Summary/Keyword: TEST-1

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Evaluation of Norudia® HbA1c Kit for Glycohemoglobin Assay (당화혈색소 측정을 위한 Norudia® HbA1c 키트의 평가)

  • Hong, Seung-Bok;Kim, Eun-Joong
    • Korean Journal of Clinical Laboratory Science
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    • v.41 no.1
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    • pp.37-41
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    • 2009
  • Measurement of hemoglobin A1c is used as an objective indicator of long-term blood glucose control in diabetic patients. We evaluated recently introduced Norudia$^{(R)}$ HbA1c (Daiichi Pure Chemical Co. Ltd, Tokyo, Japan) test reagent using enzyme method for HbA1c assay. Linearity, precision and correlation with VARIANT$^{TM}$ II Turbo HbA1c analyzer (BIO-RAD, Hercules, CA, USA) were evaluated. The reference range was determined from 201 healthy subjects. The Norudia$^{(R)}$ HbA1c test reagent was founded to be linear in a range of 5.6% to 14.0% ($r^2=0.9885$). The within-run and between-day precision were 0.954% and 1.03% for low level (HbA1c 5.24%), 0.67% and 1.28% for high level (HbA1c 9.01%), respectively. Comparison study between Norudia$^{(R)}$ HbA1c test reagent and VARIANT$^{TM}$ II Turbo showed good correlation with a slope of 1.0489. an intercept at -0.9717, and coefficient of correlation was 0.9907. The reference range of HbA1c obtained from this reagent was 4.07-5.50%. The Norudia$^{(R)}$ HbA1c test reagent showed good linearity, precision and correlation with HbA1c analyzer with HPLC method. In addition, the exclusive analyzer is not required for assay and then this kit may be useful for HbA1c assay in clinical laboratory.

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A study of analgesic effect of Zanthoxylum bungeanum Maxim pharmacopuncture

  • Lee, Jung Hee;Lee, Yun Kyu;Lee, Hyun Jong;Lee, Bong Hyo;Kim, Jae Soo
    • Journal of Acupuncture Research
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    • v.34 no.2
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    • pp.61-74
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    • 2017
  • Objectives : This study was carried out to evaluate analgesic effects of Zanthoxylum bungeanum Maxim (ZM) pharmacopuncture on formalin-induced pains in Sprague-Dawley (SD) rats and ICR-mice. Methods : The subjects were divided 8 weeks aged rats with constant pain sensitivity into five groups; normal (treated with normal saline at Taegye (KI3) and before injected with normal saline at hindpaw), Con-1 (treated with normal saline at KI3 before injected with formalin at hindpaw), Lido-1 (treated with lidocaine at KI3), ZMWG-1 (treated with Hot water extraction pharmacopuncture of Zanthoxylum bungeanum Maxim at KI3), ZMEG-1 (treated with ethanol extraction pharmacopuncture of Zanthoxylum bungeanum Maxim at KI3). After 35 minutes, we measured ultrasonic vocalization (USV) and enzyme activities of both Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) in rat serum. In addition, Tail flick test is performed by injecting ICR mice at 5 weeks of age. And it classified into 4 groups (Con-2, Lido-2, ZMWG-2, ZMEG-2) according to the kind of drug (normal saline, lidocaine, ZMW, ZME). After each drug injection, we examined the reaction by placing the tail in water at $50^{\circ}C$. Results : ZME had analgesic effects in the early and late phase of USV during the formalin test. There were no significant differences between ZMEG-1 and Lido-1 in early and late phase of USV. Also, No significant differences observed in serum AST and ALT activity in ZMWG-1 and ZMEG-1 compared with Con-1. For tail-flick test, analgesic effect on warmth significantly increased in Lido-2 and ZMEG-2 compare to that of Con-2. Conclusion : ZME pharmacopuncture had analgesic effects on formalin-induced pain without liver toxicity. Also, tail-flick test suggest that ZME pharmacopuncture could be useful technique on analgesic effect on warmth and treatment of pains.

Development of Evaluation Technique of High Temperature Creep Characteristics by Small Punch-Creep Test Method (ll) - Boiler Header - (Sp-Creep 시험에 의한 고온 크리프 특성 평가 기술 개발(ll) - 보일러 헤더 -)

  • Baek, Seung-Se;Lee, Dong-Hwan;Ha, Jeong-Su;Yu, Hyo-Seon
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.26 no.1
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    • pp.55-60
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    • 2002
  • For the development of a new creep test technique, the availability of SP-Creep test is discussed for 1Cr-0.5Mo boiler header material. And some results are also compared with those of 2.25Cr- 1Mo steel which widely uses as boiler superheater tube. The results can be summarized as follows. The load exponents(n) obtained by SP-Creep test for 1Cr-0.5Mo steel are decreased with increasing creep temperature and the values are 15.67, 13.89, and 17.13 at 550$^{circ}C$ ,575$^{circ}C$ and 600$^{circ}C$, respectively. The temperature dependence of the load exponent is given by n = 107.19 - 0.1108T. This reason that load exponents show the extensive range of 10∼16 is attributed to the fine carbide such as M$_{23}$C$_{6}$ in lath tempered martensitic structures. At the same creep condition, the secondary creep rate of 1Cr-0.5Mo steel is lower than the 2.25Cr-1Mo steel1 due to the strengthening microstructure composed by normalizing and tempering treatments. Through a SEM observation, it can be summarized that the primary, secondary, and tertiary creep regions of SP-Creep specimen are corresponding to plastic bending, plastic membrane stretching, and plastic instability regions among the deformation behavior of four steps in SP test, respectively.y.

Efficacy of Brucella abortus strain RB51 vaccine in Korean mongrel dogs against virulent strains of B. abortus biotype 1 and B. canis

  • Hur, Jin;Baek, Byeong-Kirl
    • Korean Journal of Veterinary Service
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    • v.33 no.1
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    • pp.29-35
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    • 2010
  • This study was performed to test the hypothesis that Brucella abortus strain RB51 (SRB51) might protect Korean indigenous mongrel dog against challenge with either virulent B. abortus biotype 1 or B. canis. A total of 12 Korean mongrel dogs were divided into four groups (Group A, B, C and D). Dogs belonging to Group A and C were inoculated subcutaneously with $1{\times}10^9$ CFU of SRB51 in 1ml of sterile phosphate buffered saline (PBS). Dogs of Group B and D were inoculated subcutaneously with 1ml of sterile PBS as control. At 12 weeks post vaccination, dogs of Group A and B were challenged by oral inoculation of virulent strain of B. canis ($5.0{\times}10^9$ CFU) and dogs of Group C and D were challenged by oral inoculation of virulent strain of B. abortus biotype 1 ($4.4{\times}10^{10}$ CFU). The serum antibodies titers in all dogs were monitored at regular interval for eight weeks after challenge (AC) by standard tube agglutination test, plate agglutination test, rose bengal test, 2-mercaptoethanol rapid slide agglutination test and 2-mercaptoethanol tube agglutination test. No antibody titers in Group A and C was detected. Also, the challenge strains were not found from blood of all dogs of Group A and C from 1 week AC till the end of the experiment by culture and modified AMOS-PCR, whereas B. canis and B. abortus challenge strains were detected from blood of Group B and D, respectively. In addition, neither of two challenge bacteria was recovered from liver, spleen, kidneys, lymph nodes and reproductive tracts of Group A and C dogs after postmortem. However, B. canis and B. abortus challenge strains were isolated from these tissues of Group B and D, respectively. These data suggest that SRB51 could be a promising vaccine candidate for immunizing dogs to control canine brucellosis caused by B. canis or B. abortus.

Determination of Aflatoxin B1 in Rice, Barley, and Feed by Non-instrumental Immunochromatographic Strip-test and High Sensitive ELISA

  • Shim, Won-Bo;Kim, Jung-Sook;Kim, Ji-Young;Choi, Jin-Gil;Je, Jung-Hyun;Kuzmina, Nina Sergeevna;Eremin, Sergei Alexandrovich;Chung, Duck-Hwa
    • Food Science and Biotechnology
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    • v.17 no.3
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    • pp.623-630
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    • 2008
  • A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG strip-test and DC-ELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both methods were coincided with the amount spiked AFB1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the ICG strip-test is easier to perform and quicker, but less sensitivity than DC-ELISA. Both methods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the ICG strip-test can be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.

Flowering and Maturing Response to Seeding Date and Short-day Treatment in Vegetable Perilla (잎들깨의 개화 및 결실에 미치는 파종기와 단일처리의 영향)

  • 한상익;곽재균;오기원;배석복;김정태;곽용호
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.4
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    • pp.466-472
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    • 1997
  • Vegetable perilla, "Ipdlkkae 1"(Perilla frutescens var japonica Hara), was tested about the flowering and maturing responce in summer and winter. In summer season, it was researched about those responses according to the change of seeding date from May 15th to Oct. 15th at one month interval in the field. "Ipdlkkae 1" flowered Oct. 2nd under the day length of eleven hours and fourty-one minutes, compared with Sep. 6th (day length of twelve hours and fourty-three minutes) of "Yepsildlggae". And those responses showed that vegetable perilla was have to seeded before July 15th for two reason. The first is a unique response of perilla to day length. If perilla stay under short-day condition for some days, perilla will flower after four weeks. The second is a weather, especially frost and cold. In the test of latest seeding at Oct. 15th, the plants flowered more late than normal flowering period and they were not able to mature for frost of early winter. And this result showed that any other species, which has the characteristic of later flowering than that of "Ipdlkkae 1", could not able to mature in the field. In winter time, this species was tested about the same responses according to the change of short-day treatments. In the case of the test from May 1st (above fourteen hours day length), even if the test plants were stayed under short-day condition for more than 10 days, they were not able to mature, but flowerd. From the test of Apr. 15th, day length of thirteen hours, the plants were showed variable reaction to the short-day treatment. In this test, 11days for short-day treatment was a basic day to decide whether flowering was delayed or not. In the test from Apr. 1st, perilla seeds were able to harvest at least 5 days short-day treatment. In the final test from Mar. 15th, it had no need to take short-day treatment for harvesting of normal seeds, because the day length of that are twelve hours, which is an enough time to induce flowering and maturing, previously reported.

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Determination of Bioconcentration Factor on Carbamates (Carbamate계 농약의 생체농축계수 측정)

  • 민경진
    • Journal of Environmental Health Sciences
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    • v.20 no.4
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    • pp.80-89
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    • 1994
  • It was reported that BCF's (Bioconcentration Factor) on Carbaryl and BPMC in concentration of 1, 2, 5 and 10 ppm, previously. Carassius auratus(goldfish) was chosen as test organism. Carbamates in fish and in test water were extracted with n-hexane and acetonitrile. GC-ECD was used to detecting and quantitating of Carbamates. Also, partition coefficients were determined with Stir flask method. To evaluate environmental toxicological profiles of tested compounds, experimental concentration were 0.05, 0.1 and 0.5 ppm in contrast to previous report. It was considered that higher BCFs of BPMC due to its higher partition coefficient compared to Carbaryl. The obtained results were as follows: 1. It was possible to determine short term BCF of Carbaryl and BPMC through relatively simple procedure in environmental concentrations. 2. BCF$_3$ of Carbaryl in concentration of 0.05, 0.1 and 0.5 ppm were 4.666 $\pm$ 0.002, 3.622 $\pm$ 0.004, 1.200 $\pm$ 0.002 and BCF$_5$ were 3.897 $\pm$ 0.005, 4.219 $\pm$ 0.017 and 1.186 $\pm$ 0.054, respectively. In the case of BPMC in same condition, BCF$_3$ were 4.077 $\pm$ 0.014, 4.900 $\pm$ 0.005, 4.750 $\pm$ 0.009 and BCF$_5$ were 3.465 $\pm$ 0.010, 4.612 $\pm$ 0.011 and 4.075 $\pm$ 0.012, respectively. 3. Carbaryl concentration in fish extract was increased as increasing test concentration, but BCF were decreased as prolonging test period, especially dropped at 0.5 ppm. 4. In the case of BPMC, BCF were decreased as increasing test concentration, but the concentration in fish extract of 3-day test group was slightly higher than that of 5-day test group. 5. Higher BPMC concentration in fish extract was due to its higher partition coefficient to compared with Carbaryl. 6. Determined logP of Carbaryl and BPMC were 2.200 and 3.180. But the calculated BCF using suggested equation was so different that predict BCF. It is suggested that BCF's of Carbamates have to be determined by experiment.

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The algorithm design and the test bed construction method of processing for periodic delayed data (주기적 지연 데이터 처리를 위한 알고리즘 설계 및 테스트 베드 구축 방법)

  • Sang-hoon Koh;Ho-jin Song;Nam-ho Keum;Pil-joong Yoo;Se-kwon Oh;Young-sung Kim
    • Journal of Advanced Navigation Technology
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    • v.27 no.1
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    • pp.102-110
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    • 2023
  • The MATS(Missile Assembly Test Set) is manufactured and used to check the function of the missile during the period of development for the guided missile system, and the requirements for power and communication are managed for equipment production. The MATS developer implements software according to the proposed communication standard to guarantee the reliability of the data that communicates with the guided missile. The test bed is built and self-performance evaluation is performed after implementation. And the verification process is performed using the standard equipment. The characteristics of periodic delay for data transmission must be reflected when building a test bed. This paper describes a test bed construction method for data processing with periodic delay. Also This paper compares and evaluates the performance by changing the previously designed algorithm.

A Study of PSs Modeling of Ulchin N/P #1 by AVR Step Test (AVR 스템시험에 의한 울진 N/P 1호기 PSS 모델링 연구)

  • 김동준;문영환;전동훈;김태균
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.50 no.8
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    • pp.351-358
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    • 2001
  • This paper deals with the PSS modeling of Ulchin N/P #1 as well as the generator and excitation system modeling by utilizing the recorded data from AVR step test, which has been performed by entering small voltage signal into the AVR summing point. In addition to it. two recorded results obtained from the AVR step test with PSS sunning and without PSS running have not only been compared each other on the time domain, but also they heve been analyzed with FFT analysis on the frequency domain; thus, the desirable effects of running PSS in Ulchin N/P #1 on power system have been explicitly confirmed. Finally, the derived PSS model parameters lead to good matches between simulation results and recorded data.

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Performance Evaluation of Class I SPD Using $10/350{\mu}s$ Surge Current ($10/350{\mu}s$ 서지전류를 이용한 I 등급 SPD의 성능평가)

  • Cho, Sung-Chul;Eom, Ju-Hong;Lee, Tae-Hyung
    • Proceedings of the KIEE Conference
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    • 2007.07a
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    • pp.1466-1467
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    • 2007
  • A new parameter W/R for the class I test have been introduced in the IEC 61643-1 published in 2005. One of the possible test impulses which meets the parameters for class I test is the 10/350 [${\mu}s$] waveshape proposed in IEC61312-1. 10/350 [${\mu}s$] waveshape meets parameters for class I test in the IEC 61643-1. The impulse generator for making the 10/350 [${\mu}s$] waveshape is consist of IVG, ICG, crowbar coil and crowbar switch. The electric change Q and the specific energy W/R were measured and calculated using the exclusive use measuring software according to the formula of IEC 611643-1. We verified the correspondence between measured results using impulse generator and recommended parameters by IEC 61643-1.

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