Determination of Bioconcentration Factor on Carbamates

Carbamate계 농약의 생체농축계수 측정

It was reported that BCF's (Bioconcentration Factor) on Carbaryl and BPMC in concentration of 1, 2, 5 and 10 ppm, previously. Carassius auratus(goldfish) was chosen as test organism. Carbamates in fish and in test water were extracted with n-hexane and acetonitrile. GC-ECD was used to detecting and quantitating of Carbamates. Also, partition coefficients were determined with Stir flask method. To evaluate environmental toxicological profiles of tested compounds, experimental concentration were 0.05, 0.1 and 0.5 ppm in contrast to previous report. It was considered that higher BCFs of BPMC due to its higher partition coefficient compared to Carbaryl. The obtained results were as follows: 1. It was possible to determine short term BCF of Carbaryl and BPMC through relatively simple procedure in environmental concentrations. 2. BCF$_3$ of Carbaryl in concentration of 0.05, 0.1 and 0.5 ppm were 4.666 $\pm$ 0.002, 3.622 $\pm$ 0.004, 1.200 $\pm$ 0.002 and BCF$_5$ were 3.897 $\pm$ 0.005, 4.219 $\pm$ 0.017 and 1.186 $\pm$ 0.054, respectively. In the case of BPMC in same condition, BCF$_3$ were 4.077 $\pm$ 0.014, 4.900 $\pm$ 0.005, 4.750 $\pm$ 0.009 and BCF$_5$ were 3.465 $\pm$ 0.010, 4.612 $\pm$ 0.011 and 4.075 $\pm$ 0.012, respectively. 3. Carbaryl concentration in fish extract was increased as increasing test concentration, but BCF were decreased as prolonging test period, especially dropped at 0.5 ppm. 4. In the case of BPMC, BCF were decreased as increasing test concentration, but the concentration in fish extract of 3-day test group was slightly higher than that of 5-day test group. 5. Higher BPMC concentration in fish extract was due to its higher partition coefficient to compared with Carbaryl. 6. Determined logP of Carbaryl and BPMC were 2.200 and 3.180. But the calculated BCF using suggested equation was so different that predict BCF. It is suggested that BCF's of Carbamates have to be determined by experiment.