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Determination of Aflatoxin B1 in Rice, Barley, and Feed by Non-instrumental Immunochromatographic Strip-test and High Sensitive ELISA  

Shim, Won-Bo (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University)
Kim, Jung-Sook (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University)
Kim, Ji-Young (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University)
Choi, Jin-Gil (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University)
Je, Jung-Hyun (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University)
Kuzmina, Nina Sergeevna (Mechnikov Research Institute of Vaccine and Sera of Russian Academy of Medical Sciences)
Eremin, Sergei Alexandrovich (Division of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University)
Chung, Duck-Hwa (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University)
Publication Information
Food Science and Biotechnology / v.17, no.3, 2008 , pp. 623-630 More about this Journal
Abstract
A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG strip-test and DC-ELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both methods were coincided with the amount spiked AFB1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the ICG strip-test is easier to perform and quicker, but less sensitivity than DC-ELISA. Both methods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the ICG strip-test can be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.
Keywords
aflatoxin B1; mycotoxin; non-instrumental assay; immunochromatographic strip-test (ICG); enzyme-linked immunosorbent assay (ELISA);
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
Times Cited By SCOPUS : 3
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