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A Simple Culture Device for the Culture and Transportation of Mouse Oocytes and Embryos in vitro(I) (생쥐난자의 단기간 체외배양과 수송을 위한 Simple Culture Device)

  • Cho, Wan-Kyoo;Yoon, Yong-Dal
    • The Korean Journal of Zoology
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    • v.22 no.4
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    • pp.175-183
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    • 1979
  • This simple culture device is based on the microtube culture method (Cho, 1974). The microtube culture system was placed in a plastic tube (Falcon, 12 $\\times$ 75 mm) and the transporting system used in the present study were Trans-Temp 310 (Royal Industries, 209 D 135-1). The simple culture device in the Trans-Temp 310 seems to be an available method for short term culture and some distance transportation of oocytes and embryos.

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A Study on the Power Loss Simulation of IGBT for HVDC Power Conversion System

  • Cho, Su Eog
    • Journal of the Korean Society of Industry Convergence
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    • v.24 no.4_1
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    • pp.411-419
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    • 2021
  • In this study, IGBT_Total_Loss and DIODE_Total_Loss were used to analyze the slope of the junction temperature for each section for temperature and duty variables in order to simply calculate the junction temperature of the power semiconductor (IGBT). As a result of the calculation, IGBT_Max_Junction_Temp and DIODE_Max_Junction_Temp form a proportional relationship with temperature for each duty. This simulation data shows that the power loss of a power semiconductor is calculated in a complex manner according to the current dependence index, voltage dependence index, and temperature coefficient. By applying the slope for each condition and section, the junction temperature of the power semiconductor can be calculated simply.

Optimization Condition of Trace Analysis of Fuel Oxygenated Compounds Using The Design of Experiment (DOE) in Solid-Phase Microextraction with GC/FID (고체상미량분석법(SPME-GC/FID)에서 실험계획법을 이용한 연료첨가제 미량분석의 최적조건)

  • An, Sang-Woo;Lee, Si-Jin;Chang, Soon-Woong
    • Journal of Soil and Groundwater Environment
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    • v.15 no.1
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    • pp.9-18
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    • 2010
  • In this study, Solid-phase micro-extraction (SPME) with Gas Chromatograph using Flame Ionization Detector (GC/FID) was studied as a possible alternative to liquid-liquid extraction for the analysis of Methyl tert-butyl ether (MTBE) and Tertiary-butyl ether (TBA) in water and an optimization condition of trace analysis of MTBE and TBA using the design of experiment (DOE) was described. The aim of our research was to apply experimental design methodology in the optimization condition of trace analysis of fuel oxygenated compounds in soil-phase microextraction with GC/FID. The reactions of SPME were mathematically described as a function of parameters of Temp ($X_1$), Volume ($X_2$), Time ($X_3$) and Salt ($X_4$) being modeled by the use of the partial factorial designs, which was used for fitting 2nd order response surface models and was alternative to central composite designs. The model predicted agreed with the experimentally observed result ($Y_1$(MTBE, $R^2$ = 0.96, $Y_2$ (TBA, $R^2$ = 0.98)). The estimated ridge of the expected maximum responses and optimal conditions for MTBE and TBA were 278.13 and (Temp ($X_1$) = $48.40^{\circ}C$, Volume ($X_2$) = 73.04 mL, Time ($X_3$) = 11.51 min and Salt ($X_4$) = 12,50 mg/L), and 127.89 and (Temp ($X_1$) = $52.12^{\circ}C$, Volume ($X_2$) = 88.88mL, Time ($X_3$) = 65.40 min and Salt ($X_4$) = 12,50 mg/L), respectively.

Correlation Analysis between Phenology of Salix spp. and Meteorological Factors (버드나무류 (Salix spp.)의 계절학적 특성과 주요 기상요인 상관분석)

  • Kim, Seong-Bo;Kim, Ji Yoon;Im, Ran-Young;Do, Yuno;Park, Hee-Sun;Joo, Gea-Jae;Kim, Gu-Yeon
    • Journal of Environmental Science International
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    • v.22 no.12
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    • pp.1633-1641
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    • 2013
  • The objective of this study was to analyze correlation between phenological characteristics of Salix spp. and meteorological factors in the Upo wetlands. Phenology of Salix subfragilis Andersson and Salix chaenomeloides Kimura was monitored from 2007 to 2012. Meteorological variables were monitored by Korea Meteorological Administration (Hap-chon). Average date of flowering, fruiting, seed dispersion was 86, 113, 136 days for S. subfragilis and 112, 140, 164 days for S. chaenomeloides as Julian days. Flowering of S. subfragilis and S. chaenomeloides were correlated with daily mean air temp. in March (r=-0.92, r=-0.85, p<0.05). Fruiting of S. subfragilis was correlated with total precipitation between Jan and March of previous year (r=-0.90, p<0.01), however, the fruiting of S. chaenomeloides was highly correlated with max. temp. in Jan of previous year (r=0.99, p<0.01). Seed dispersion of both species is correlated with min. temp. in Feb. Phenology monitoring will contribute to understanding Salix spp. response against climate change.

Changes of composition during storage of Ginseng drink product (인삼드링크제품의 저장중 성분변화)

  • Joo, Hyun-Kyu;Jung, Dong-Kon;Kim, Nam-Dae
    • Applied Biological Chemistry
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    • v.34 no.4
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    • pp.339-343
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    • 1991
  • The objective of this study was to evalute changes of $CO_2$ pressure, pH, precipitation, brix, free sugar, colority, crude saponin and ginsenoside contents of Ginseng Drink Product for ten months at different temperatures $(room\;temp.,\;35^{\circ}C$ and $50^{\circ}C)$. The results are as follows : $CO_2$ pressure was decreased after nine month storage at room temp., six months at $35^{\circ}C$ and three months at $50^{\circ}C$. pH and brix nearly constant at various storage temperatures and periods. Precipitation was appeared after five months storage at room temp., two months at $35^{\circ}C$ and one month at $50^{\circ}C$. Sucrose content was decrease, while glucose and fructose contents were increased at high temperature and long period of stroage. Colority and panaxatrial(PT) saponin contents were increased, while panaxadiol(PD) saponin was increased at high temperature and long period of stroage.

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Comparison of fracture strength after thermomechanical aging between provisional crowns made with CAD/CAM and conventional method

  • Reeponmaha, Tanapon;Angwaravong, Onauma;Angwarawong, Thidarat
    • The Journal of Advanced Prosthodontics
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    • v.12 no.4
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    • pp.218-224
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    • 2020
  • PURPOSE. The objectives of this study were to evaluate the fracture strength and fracture patterns of provisional crowns fabricated from different materials and techniques after receiving stress from a simulated oral condition. MATERIALS AND METHODS. A monomethacrylate-based resin (Unifast Trad) and a bis-acryl-based (Protemp 4) resin were used to fabricate provisional crowns using conventional direct technique. A milled monomethacrylate resin (Brylic Solid) and a 3D-printed bis-acrylate resin (Freeprint Temp) were chosen to fabricate provisional crowns using the CAD/CAM process. All cemented provisional crowns (n=10/group) were subjected to thermal cycling (5,000 cycles at 5°-55℃) and cyclic occlusal load (100 N at 4 Hz for 100,000 cycles). Maximum force at fracture was tested using a universal testing machine. RESULTS. Maximum force at fracture (mean ± SD, N) of each group was 657.87 ± 82.84 for Unifast Trad, 1125.94 ± 168.07 for Protemp4, 953.60 ± 58.88 for Brylic Solid, and 1004.19 ± 122.18 for Freeprint Temp. One-way ANOVA with Tamhane post hoc test showed that the fracture strength of Unifast Trad was statistically significantly lower than others (P<.01). No statistically significant difference was noted among other groups. For failure pattern analysis, Unifast Trad and Brylic Solid showed less damage than Protemp 4 and Freeprint Temp groups. CONCLUSION. Provisional crowns fabricated using the CAD/CAM process and the conventionally fabricated bis-acryl resins exhibited significant higher fracture strength compared to conventionally fabricated monomethacrylate resins after the aging regimen. Therefore, CAD/CAM milling and 3D printing of provisional restorations may be good alternatives for long term provisionalization.

Estimation method of heat flux at tube bank exposed to high temperature flue gas in large scale coal fired boilers (보일러 내부 고온가스에 노출된 전열 튜브에서의 열유속 평가 방법)

  • Jung, Jae-Jin;Song, Jung-Il
    • 한국태양에너지학회:학술대회논문집
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    • 2009.11a
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    • pp.259-264
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    • 2009
  • Most of the fossil power plants firing lower grade coals are challenged with maintaining good combustion conditions while maximizing generation and minimizing emissions. In many cases significant derate, availability losses and increase in unburned carbon levels can be attributed to poor combustion conditions as a result of poorly controlled local fuel and air distribution within the boiler furnace. The poor combustion conditions are directly related to the gas flow deviation in upper furnace and convection tube-bank but a less reported issue related to in large-scale oppose wall fired boilers. In order to develop a on-line combustion monitoring system and suggest an alternative heat flux estimation method at tube bank, which is very useful information for boiler design tool and blower optimizing system, field test was conducted at operating power boiler. During the field test the exhaust gases' temperature and tube metal temperature were monitored by using a spatially distributed sensors grid which located in the boiler's high temperature vestibule region. At these locations. the flue gas flow is still significantly stratified, and air in-leakage is minimal which enables tracing of poor combustion zones to specific burners and over-fire air ports. Test results showed that the flue gas monitoring method is more proper than metal temperature distribution monitoring for real time combustion monitoring because tube metal temp. distribution monitoring method is related to so many variables such as flue gas, internal flow unbalance, spray etc., Heat flux estimation at the tube bank with flue gas temp. and metal temp. data can be alternative method when tube drilling type sensor can't able to use.

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Seasonal Acclimatization in Summer versus Winter to Changes in the Sweating Response during Passive Heating in Korean Young Adult Men

  • Lee, Jeong-Beom;Kim, Tae-Wook;Min, Young-Ki;Yang, Hun-Mo
    • The Korean Journal of Physiology and Pharmacology
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    • v.19 no.1
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    • pp.9-14
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    • 2015
  • We investigated the sweating response during passive heating (partial submersion up to the umbilical line in $42{\pm}0.5^{\circ}C$ water, 30 min) after summer and winter seasonal acclimatization (SA). Testing was performed in July during the summer, 2011 [summer-SA; temp, $25.6{\pm}1.8^{\circ}C;$ relative humidity (RH), $82.1{\pm}8.2%$] and in January during the winter, 2012 (winter-SA; temp, $-2.7{\pm}2.9^{\circ};$ RH, $65.0{\pm}13.1%$) in Cheonan ($126^{\circ}52^{\prime}N$, 33.38'E), Republic of Korea. All experiments were carried out in an automated climatic chamber (temp, $25.0{\pm}0.5^{\circ}C$: RH, $60.0{\pm}3.0%$). Fifteen healthy men (age, $23.4{\pm}2.5$ years; height, $175.0{\pm}5.9cm;$ weight, $65.3{\pm}6.1kg$) participated in the study. Local sweat onset time was delayed during winter-SA compared to that after summer-SA (p<0.001). Local sweat volume, whole body sweat volume, and evaporative loss volume decreased significantly after winter-SA compared to those after summer-SA (p<0.001). Changes in basal metabolic rate increased significantly after winter-SA (p<0.001), and tympanic temperature and mean body temperature were significantly lower after summer-SA (p<0.05). In conclusion, central sudomotor acitivity becomes sensitive to summer-SA and blunt to winter-SA in Rebubic of Korea. These results suggest that the body adjusts its temperature by economically controlling the sweating rate but does not lower the thermal dissipation rate through a more effective evaporation scheme after summer-SA than that after winter-SA.

Fabrication and Characteristics of ZnO TFTs for Flexible Display using Low Temp Process (Flexible Display용 Low Temp Process를 이용한 ZnO TFT의 제작 및 특성 평가)

  • Kim, Young-Su;Kang, Min-Ho;Nam, Dong-Ho;Choi, Kang-Il;Lee, Hi-Deok;Lee, Ga-Won
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.22 no.10
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    • pp.821-825
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    • 2009
  • Recently, transparent ZnO-based TFTs have attracted much attention for flexible displays because they can be fabricated on plastic substrates at low temperature. We report the fabrication and characteristics of ZnO TFTs having different channel thicknesses deposited at low temperature. The ZnO films were deposited as active channel layer on $Si_3N_4/Ti/SiO_2/p-Si$ substrates by RF magnetron sputtering at $100^{\circ}C$ without additional annealing. Also, the ZnO thin films deposited at oxygen partial pressures of 40%. ZnO TFTs using a bottom-gate configuration were investigated. The $Si_3N_4$ film was deposited as gate insulator by PE-CVD at $150^{\circ}C$. All Processes were processed below $150^{\circ}C$ which is optimal temperature for flexible display and were used dry etching method. The fabricated devices have different threshold slop, field effect mobility and subthreshold slop according to channel thickness. This characteristics are related with ZnO crystal properties analyzed with XRD and SPM. Electrical characteristics of 60 nm ZnO TFT (W/L = $20\;{\mu}m/20\;{\mu}m$) exhibited a field-effect mobility of $0.26\;cm^2/Vs$, a threshold voltage of 8.3 V, a subthreshold slop of 2.2 V/decade, and a $I_{ON/OFF}$ ratio of $7.5\times10^2$.