• Journal of Microbiology and Biotechnology
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• v.20 no.6
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• pp.1006-1010
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• 2010

Pseudomonas putida JM37 metabolized glyoxylate at a specific rate of 55 g/g dry biomass/day. In order to investigate their role, three genes encoding enzymes that are potentially involved in the conversion of glyoxylate were disrupted; namely, tartronate semialdehyde synthase (gcl), malate synthase (glcB), and isocitrate lyase (aceA). Strains with transposon insertion in either of these genes were isolated from a 50,000 clone library employing a PCR-guided enrichment strategy. In addition, all three double mutants were constructed via targeted insertion of a knock-out plasmid. Neither mutation of gcl, glcB, and aceA nor any of the respective double mutations influenced glyoxylic acid conversion, indicating that P. putida JM37 may possess other enzymes and pathways for glyoxylate metabolism.

• Transactions of the Korean Society of Automotive Engineers
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• v.10 no.4
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• pp.216-222
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• 2002

Brake Moan is a laud and strong noise occurring at any vehicle speed over 2 mph as a low frequency in below 600Hz. In this study, we targeted to shift the unstable mode that causes the brake moan from the moats frequency range to sufficiently higher frequency range to avoid the moan phenomenon. We simulated the finite element model and found out the nodes in which the brake moan occurs the most and we regarded the boundary and its relationship between the brake pad and the rotor as a spring coefficient k. With the binary set of the spring coefficient k, we finally used genetic algorithm (GA) to get the optimal topology of the brake pad and its shape to avoid the brake moan. The final result remarkably shows that genetic algorithm can be used in topology optimization procedures requiring complex eigenvalue problems.

• 한국항공운항학회:학술대회논문집
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• 2016.05a
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• pp.162-177
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• 2016

The government conducted an aviation expert training project in 2013. But unfortunately, only pilots and international experts were targeted and aircraft maintenance engineers that are the most important to safety were not trainee to the training courses. More importantly, the more the absolute number of aircrafts increases, the more demand for aircraft maintenance engineers are going up as well. Through the evaluation indicators for the Government-Sponsored Program Fostering Aviation Personnel(aircraft maintenance engineers). it figured out factors associated with the Government-Sponsored Program Fostering Aircraft Maintenance Engineers and it suggested a baseline data necessary to inspect and manage professional training institutions. Thanks to the development of the evaluation indicators, it is highly expected that difference among institutions will be narrowed, elevated level of management of the institutions will help foster and yield satisfactory aircraft maintenance engineers.

• 한국유가공학회:학술대회논문집
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• 2002.04a
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• pp.45-55
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• 2002

Recent developments in the application of molecular biology to food grade lactic acid bacteria (LAB) have shown that it could be feasible to engineer metabolic pathways to either enhance specific metabolic fluxes or to divert metabolites for the production of different or new end products. This engineering requires detailed knowledge of enzymes involved in metabolism and regulation within the targeted organism but little works have been done in this area. During biochemical and molecular characterisation of lactic bacterial enzymes, some of probiotic Lactobacillus and Bifidobacterium species were found to be very useful for food, nutraceutical and pharmaceutical industries. The enzymes are usually intracellular and the yields are very low to be useful for industrial applications. Among many enzymes and proteins of lactic bacteria studied, some of our gene cloning achievements have contributed to overproduction of lactic bacterial enzymes such as peptidases, esterases, lactases, bile salt hydrolases and linoleate isomerases for foods and nutraceuticals.

• 한국방재학회:학술대회논문집
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• 2011.02a
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• pp.3-8
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• 2011

In April 2010, Japan Cabinet Office has published the first countermeasure report for severe flood disasters. This report showed various flood-disaster scenarios and factors that widened damages. One of important suggestions was to transmit precious information for long-distance evacuation. So far, local municipalities have made Flood Hazard Map to inform resident risk and evacuation. In this paper, cognition and effectiveness of a flood hazard map in the down ARAKAWA river Tokyo were measured by social questionnaire survey. In conclusion, there were 3 factors to effect validity of a flood hazard map. There were (1) commitment to their neighborhood organization, (2) experience of Kathleen typhoon in 1947 and (3) level of using targeted river. As results, a logical diagram about a flood hazard map perception was drawn and discussed from a view of community-based approach.

• Mass Spectrometry Letters
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• v.6 no.2
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• pp.31-37
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• 2015

Phosphorylation upon protein is well known to a key regulator that implicates in modulating many cellular processes like growth, migration, and differentiation. Up to date, grafting of multidimensional separation techniques onto advanced mass spectrometry (MS) has emerged as a promising tool for figuring out the biological functions of phosphorylation in a cell. However, advanced MS-based phosphoproteomics is still challenging, due to its intrinsic issues, i.e., low stoichiometry, less susceptibility in positive ion mode, and low abundance in biological sample. To overcome these bottlenecks, diverse techniques (e.g., SCX, HILIC, ERLIC, IMAC, TiO₂, etc.) are continuously developed for on-/off-line enrichment of phosphorylated protein (or peptide) from biological samples, thereby helping qualitative/quantitative determination of phosphorylated protein and its phosphorylated sites. In this review, we introduce to the overall views of enrichment tools that are universally used to selectively isolate targeted phosphorylated protein (or peptide) from ordinary ones before MS-based phospoproteomic analysis.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.4
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• pp.784-789
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• 2007

This paper presents an implementation of a new type of architecture to improve an overflow problem on the network buffer. Each receiver channel of network system stores the message in its own buffer. If some receiver channel receives many messages, buffer overflow problem may occur for the channel. This paper proposes a network controller that implements a receiver channel with shared-memory to save all of the received messages from the every incomming channels. The proposed architecture is applied to ARINC-429, a real-time control network for commercial avionics system. For verifying performance of the architecture, ARINC-429 controller is designed using a SOPC platform, designed by Verilog and targeted to Xilinx Virtex-4 with a built-in PPC405 core.

• Journal for History of Mathematics
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• v.26 no.4
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• pp.301-320
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• 2013

Although many people have recognized the importance of history of mathematics in mathematics education, there are few studies that history and mathematics educations are connected. In this paper, we present and discuss the ways of introducing the instruction with mathematics machines. This instruction use the history of mathematics as part of a class not as a tool to stimulate students' interest but as a goal that it can be targeted mathematical itself. To do this, we first analyze the characteristics of applying history of mathematics in mathematical education, second, describe the meaning and the educational value of mathematical machines, and finally explained the way of applying history of mathematics with mathematics machines. The Instruction of history of mathematics with mathematics machines has advantages that practice (manipulation and experiment) and theory (elaboration of definition, production of conjectures and constructions of proofs) are interlaced within a historic-cultural perspective.

• Clinical and Experimental Pediatrics
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• v.61 no.4
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• pp.101-107
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• 2018

Recent advances in genetics have determined that a number of epilepsy syndromes that occur in the first year of life are associated with genetic etiologies. These syndromes range from benign familial epilepsy syndromes to early-onset epileptic encephalopathies that lead to poor prognoses and severe psychomotor retardation. An early genetic diagnosis can save time and overall cost by reducing the amount of time and resources expended to reach a diagnosis. Furthermore, a genetic diagnosis can provide accurate prognostic information and, in certain cases, enable targeted therapy. Here, several early infantile epilepsy syndromes with strong genetic associations are briefly reviewed, and their genotype-phenotype correlations are summarized. Because the clinical presentations of these disorders frequently overlap and have heterogeneous genetic causes, next-generation sequencing (NGS)-based gene panel testing represents a more powerful diagnostic tool than single gene testing. As genetic information accumulates, genetic testing will likely play an increasingly important role in diagnosing pediatric epilepsy. However, the efforts of clinicians to classify phenotypes in nondiagnosed patients and improve their ability to interpret genetic variants remain important in the NGS era.

• Journal of Microbiology and Biotechnology
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• v.28 no.5
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• pp.821-825
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• 2018

A copy number amplification system for yeast artificial chromosomes (YACs) was combined with simultaneous overexpression of genes integrated into a YAC. The chromosome VII (1,105 kb) was successfully split to 887 kb, 44 kb containing the element for copy number amplification, and a 184-kb split-YAC. The 44-kb split-mini YAC was amplified a maximum of 9-fold, and the activity of the reporter enzymes integrated into the split-mini YAC increased about 5-7-fold. These results demonstrate that the mini-YAC containing a targeted chromosome region can be readily amplified, and the specific genes in the mini-YAC could be overexpressed by increasing the copy number.