• Bulletin of the Korean Chemical Society
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• v.33 no.2
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• pp.651-656
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• 2012

Cationic immunoliposomes were prepared by conjugation of Fab' fragments of the recombinant humanized monoclonal antibody (HuCC49) against tumor-associated glycoprotein (TAG)-72 to sterically unilamella liposomes. The cationic immunoliposomes are composed of cationic lipid (O,O'-dimyristyl-N-lysyl aspartate, DMKD), cholesterol, and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethyleneglycol)$_{2000}$] (DPPE-PEG-maleimide) with a molar ratio of 0.5:0.47:0.03. Plasmid DNA was effectively condensed by addition of transferrin (Tf) during the formation of anti-TAG-72 PEG-immunolipoplexes (PILPs). These anti-TAG-72 PILPs were able to adhere to the surface of TAG-72-overexepressing LS174T human colon cancer cells more effectively than conventional liposomes, thereby facilitating gene delivery in vitro. Furthermore, intravenous administration of the anti-TAG-72 PILPs into the tumor-carrying mice exhibited efficient localization of the reporter gene in the tumor tissues.

• Journal of Microbiology
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• v.45 no.6
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• pp.572-577
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• 2007

To enhance therapeutic potential of murine monoclonal antibody, humanization by CDR grafting is usually used to reduce immunogenic mouse residues. Most humanized antibodies still have mouse residues critical for antigen binding, but the mouse residues may evoke immune responses in humans. Previously, we constructed a new humanized version (AKA) of mouse CC49 antibody specific for tumor-associated glycoprotein, TAG-72. In this study, to select a completely human antibody light chain against TAG-72, guided selection strategy using phage display was used. The heavy chain variable region (VH) of AKA was used to guide the selection of a human TAG-72-specific light chain variable region (VL) from a human VL repertoire constructed from human PBL. Most of the selected VLs were identified to be originated from the members of the human germline VK1 family, whereas the VL of AKA is more homologous to the VK4 family. Competition binding assay of the selected Fabs with mouse CC49 suggested that the epitopes of the Fabs overlap with that of CC49. In addition, they showed better antigen-binding affinity compared to parental AKA. The selected human VLs may be used to guide the selection of human VHs to get completely human anti-TAG72 antibody.

• The Korean Journal of Nuclear Medicine
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• v.26 no.2
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• pp.371-379
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• 1992

악성종양의 진단 및 치료에 있어서 특정 종양에 대한 항체를 이용하는 연구가 활발히 진행되고 있다. 단세포군항체를 이용한 방사면역신티그라피로 암의 조기 진단 및 영상화가 가능하고 나아가 방사면역치료는 암의 선택적 치료에 도움이 될 수 있다. 위암은 우리나라에서 가장 흔한 악성종양으로 방사면역신티그라피와 방사면역치료법이 새로운 방법으로 모색되고 있다. 이러한 진단 및 치료법의 성공여부를 결정하는 중요한 인자의 하나가 종양조직내에서 종양관련 항원의 농도와 분포이다. 따라서 본 연구에서는 단세포군 항체를 이용한 방사면역학적 방법의 임상 이용을 위한 기초 연구의 일환으로 in vitro quantitative autoradiography를 이용하여 종양 관련항원인 TAG-72와 CEA의 위암조직내 농도 및 분포를 측정하였다. 33예의 위암조직에서 얻은 동결절편을 $1.3\sim83.3$ nmol/liter의 $^{125}I$ 표지 항 TAG-72 단세포군 항체 B-72.3과 항 CEA 단세포군 항체 CEA-79로 반응시킨 후 이 표본들의 자가방사법 디지탈 영상을 H & E 염색과 immunoperoxidase염색 표본과 비교하였으며, 특정 단세포군 항체의 결합에 대한 컴퓨터 분석으로 조직내 항원의 농도와 분포를 측정하였다. TAG-72는 25예(75.7%)의 조직에서 검출되었으며 그 농도는 $8.4\sim525.3$ pmol/gram이었고, CEA는 32예 (96.9%)에서 검출되었으며 그 농도는 $8.8\sim592.9$ pmol/gram 이었다. CEA의 위암 조직내 발현농도는 중앙치가 101.7 pmol/gram 으로 TAG-72의 중앙치인 27.9 pmol/gram 보다 높았다. TAG-72의 조직내 분포는 41.4%에서 병변 부위의 암세포 분포와 일치하였고, CEA의 분포는 병변 부위의 80.5%에서 암세포와 일치하는 소견을 나타내었다. TAG-72의 농도는 점액성 선종(mucinous adenocarcinoma)과 점액함유 선종(mucin containing adenocarcinoma)에서 다른 선종보다 더 높았다. CEA의 농도는 위암의 병리학적 종류에 따른 유의한 차이가 없었다. 이상의 결과로 위암조직에서 TAG-72와 CEA항원이 다양하게 발현됨을 알 수 있었고 CEA는 TAG-72 보다 더 빈번하게 균일한 분포로 발현하는 것으로 나타났다.

• The monthly packaging world
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• no.6 s.182
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• pp.60-72
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• 2008

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.48 no.10
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• pp.1-5
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• 2011

A magnetic resonant coil system for enhancement of wireless power transfer efficiency of NFC devices was proposed. The NFC system consists of resonant coils arrange between source coil and device coil. The effects of resonant coil was measured using a 13.56MHz RFID reader and tag system and simulated by 3D RF simulator. The measurement results from RFID reader and tag show that the maximum distance of signal transmission is increased by 96.72% and received voltage of RFID tag is grew by 17.95% thanks to the magnetic resonant coils.

• The Journal of Korean Academy of Prosthodontics
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• v.25 no.1
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• pp.7-16
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• 1987

The purpose of this study was to compare the resin tag length of 3 composite resin cements: Panavia, Compspan, Conclude, and the resin tag length of Panavia cemented to daliva-contaminated enamel using scanning electron microscopy. The following conclusions can be drawn from this study. 1. The resin tag length of panavia was $8.29{\mu}m$, compsan $8.72{\mu}m$, conclude $7.74{\mu}m$, and Panavia cemented to saliva-contaminated enamel $3.92{\mu}m$. 2. No significant difference of resin tag length between Panavia, Comspan and Conclude could be observed. 3. Saliva contamination of etched enamel surface decreases the wettability of composite resin cement.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.19 no.3
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• pp.61-72
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• 2009

To guarantee security between the tag and back-end server and implementation efficiency in low power tag, we propose two typed mutual authentication protocols in RFID system. One is static-ID authentication scheme which is well suitable in distributed server environments. The other is dynamic-ID scheme which is additively satisfied forward security. In proposed scheme, it does not need any random number generator in tag and requires only one(maximally three) hash operation(s) in tag or server to authenticate each other. Furthermore, we implement the proposed schemes in RFID smart card system and verify its normal operations.

• The Journal of the Korea Contents Association
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• v.9 no.1
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• pp.62-72
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• 2009

In this paper, we propose an application of RFID(Radio Frequency Identification) tag to food wastes disposal system of Cheongju city. The existing system of Cheongju city is monthly fixed amount levy system. The weak point of this system must pay a same commission in spite of each other different food wastes quantity. Also the accurate occurrence quantity of the food wastes and present condition grasp of control are impossible. It will not be able to reflect to a policy decision because does not manage the control of the food waste efficiently. On the other hand, when a food wastes measuring system using RFID is executed tag, it will manage and analyze automatically the obtained data. And collecting, transporting and handling of the food wastes come to be very easy. Also the reproduction and embezzlement of the tag are impossible. As a result of this system, it brings the decrement of the food waste. Therefore the economic effect could be come true and the food wastes is controlled environment-friendly. The food wastes disposal system using RFID tag will be able to contribute in u-city constructions with RFID/USN technology which pursues from the government.

• 대한핵의학회:학술대회논문집
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• 1990.04a
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• pp.208.1-208.1
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• 1990

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.11
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• pp.4991-4998
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• 2016

Objective: This research was conducted to explore mechanisms behind the potency of quercetin in inhibiting colon cancer induced in an experimental model. Materials and Methods: Forty adult male rats of Wistar strain were distributed into 4 groups; a negative control group, a colon cancer bearing group, a quercetin-treated group and a 5-fluorouracil (5-FU)-treated group. Serum TAG72 and GAL3 levels were quantified by ELISA. Colonic Wnt5a and Axin-1 gene expression was estimated by PCR. In addition, colonic tissues were subjected to immunohistochemical examination of Bax expression and histological investigation of histopathological alterations. Results: Quercetin elicited significant reduction in serum TAG72 and GAL3 levels, in addition to significant suppression of colonic Wnt5a gene expression and amplification of colonic Axin-1 gene expression. Also, it caused moderate positive reaction for Bax in mucosal epithelium. Conclusion: The present research provides experimental evidence about the activity of quercetin in the colon cancer of rats. Inhibitory effects on cancer development might be ascribable to regulatory action on Wnt signaling and induction of apoptosis.