• BMB Reports
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• 제50권10호
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• pp.522-527
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• 2017

A large number of transcriptional activation domains (TADs) are intrinsically unstructured, meaning they are devoid of a three-dimensional structure. The fact that these TADs are transcriptionally active without forming a 3-D structure raises the question of what features in these domains enable them to function. One of two TADs in human glucocorticoid receptor (hGR) is located at its N-terminus and is responsible for ~70% of the transcriptional activity of hGR. This 58-residue intrinsically-disordered TAD, named tau1c in an earlier study, was shown to form three helices under trifluoroethanol, which might be important for its activity. We carried out heteronuclear multi-dimensional NMR experiments on hGR tau1c in a more physiological aqueous buffer solution and found that it forms three helices that are ~30% pre-populated. Since pre-populated helices in several TADs were shown to be key elements for transcriptional activity, the three pre-formed helices in hGR tau1c delineated in this study should be critical determinants of the transcriptional activity of hGR. The presence of pre-structured helices in hGR tau1c strongly suggests that the existence of pre-structured motifs in target-unbound TADs is a very broad phenomenon.

• 대한치과의사협회지
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• 제52권9호
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• pp.532-540
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• 2014

In contemporary orthodontic treatment skeletal temporary anchorage devices (TADs) are routinely used as an anchorage reinforcement to provide improved anchorage control with reduced requirement for patient's compliance. For past few decades, various types of TADs have been explored and their clinical application has been expanded. Therefore, the purpose of this article is to present three major types of orthodontic skeletal anchorage devices and discuss their rationale, clinical procedure, insertion site, and potential complications as well as their management.

• 대한치과교정학회지
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• 제42권3호
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• pp.110-117
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• 2012

Objective: In this study, we measured the cortical bone thickness in the mandibular buccal and lingual areas using computed tomography in order to evaluate the suitability of these areas for application of temporary anchorage devices (TADs) and to suggest a clinical guide for TADs. Methods: The buccal and lingual cortical bone thickness was measured in 15 men and 15 women. Bone thickness was measured 4 mm apical to the interdental cementoenamel junction between the mandibular canine and the 2nd molar using the transaxial slices in computed tomography images. Results: The cortical bone in the mandibular buccal and lingual areas was thicker in men than in women. In men, the mandibular lingual cortical bone was thicker than the buccal cortical bone, except between the 1st and 2nd molars on both sides. In women, the mandibular lingual cortical bone was thicker in all regions when compared to the buccal cortical bone. The mandibular buccal cortical bone thickness increased from the canine to the molars. The mandibular lingual cortical bone was thickest between the 1st and 2nd premolars, followed by the areas between the canine and 1st premolar, between the 2nd premolar and 1st molar, and between the 1st molar and 2nd molar. Conclusions: There is sufficient cortical bone for TAD applications in the mandibular buccal and lingual areas. This provides the basis and guidelines for the clinical use of TADs in the mandibular buccal and lingual areas.

• 대한치과교정학회지
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• 제49권2호
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• pp.106-115
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• 2019

Objective: This study aimed to evaluate the effects of force loading on root damage caused by contact with temporary anchorage devices (TADs) during orthodontic treatment and to examine the repair process 4, 8, and 12 weeks after TAD contact by micro-computed tomography (CT). Methods: We enrolled 42 volunteers who required bilateral upper first premolar extractions. The experimental study design was as follows. For both first premolars, cantilever springs were placed, and then TADs were immediately inserted between the premolars of all volunteers. According to the removal order of the appliances, the participants were divided into the TAD group (Group T: n = 21, only TAD removal) and the spring group (Group S: n = 21, only spring removal). A splitmouth design was adopted in both groups as follows. For each volunteer, the left premolars were extracted 4, 8, or 12 weeks after TAD-root contact. The right premolars were extracted immediately after contact in both groups (Groups T-C and S-C) and used as positive controls. Resorption volumes and numbers of craters were determined by micro-CT. Results: The numbers of resorption craters were higher in Group T than in Group S at 8 and 12 weeks (p < 0.01). Crater volumes were higher in Group T than in Group S at 4 and 12 weeks (p < 0.01, both). Conclusions: Root injury was not completely repaired 12 weeks after root-TAD contact, even when the TADs were removed in cases of continuous force application.

• 대한치과교정학회지
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• 제42권6호
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• pp.291-296
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• 2012

Objective: The purposes of this study were to measure the palatal soft tissue thickness at popular placement sites of temporary anchorage devices (TADs) by cone-beam computed tomography (CBCT) and evaluate the age, gender, and positional differences in this parameter. Methods: The study sample consisted of 23 children (10 boys and 13 girls; mean age, $10.87{\pm}1.24$ years; range, 6.7 to 12.6 years) and 27 adults (14 men and 13 women; mean age, $21.35{\pm}1.14$ years; range, 20.0 to 23.8 years). Nine mediolateral and nine anteroposterior intersecting reference lines were drawn on CBCT scans of the 50 subjects, and the resultant measurement areas were designated according to their mediolateral (i.e., lateral, medial, and sutural) and anteroposterior (i.e., anterior, middle, and posterior) positions. Repeated-measures analysis of variance was performed to analyze intragroup and intergroup differences. Results: No significant age and gender differences were found (p = 0.309 and 0.124, respectively). Further, no significant anteroposterior change was observed (p = 0.350). However, the lateral area presented the thickest soft tissue whereas the sutural area had the thinnest soft tissue (p < 0.001). Conclusions: Clinical selection of the placement sites of TADs should be guided by knowledge of the positional variations in the palatal soft tissue thickness in addition to other contributing factors of TAD stability.

• 대한치과교정학회지
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• 제49권5호
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• pp.279-285
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• 2019

Objective: This study evaluated the efficiency of anchorage provided by temporary anchorage devices (TADs) in maxillary bicuspid extraction cases during retraction of the anterior teeth using a fixed appliance. Methods: Patients aged 12 to 50 years with malocclusion for which bilateral first or second maxillary bicuspid extractions were indicated were included in the study and randomly allocated to the TAD or control groups. Retraction of the anterior teeth was achieved using skeletal anchorage in the TAD group and conventional dental anchorage in the control group. A computed tomography (CT) scan was performed after alignment of teeth, and a second CT scan was performed at the end of extraction space closure in both groups. A three-dimensional superimposition was performed to visualize and quantify the maxillary first molar movement during the retraction phase, which was the primary outcome, and the stability of TAD movement, which served as the secondary outcome. Results: Thirty-four patients (17 in each group) underwent the final analysis. The two groups showed a significant difference in the movement of the first maxillary molars, with less significant anchorage loss in the TAD group than that in the control group. In addition, TAD movement showed only a slight mesial movement on the labial side. On the palatal side, the mesial TAD movement was greater. Conclusions: In comparison with conventional dental anchorage, TADs can be considered an efficient source of anchorage during retraction of maxillary anterior teeth. TADs remain stable when correctly placed in the bone during the anterior tooth retraction phase.

• Molecules and Cells
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• 제44권11호
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• pp.805-829
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• 2021

CCCTC-binding factor (CTCF) critically contributes to 3D chromatin organization by determining topologically associated domain (TAD) borders. Although CTCF primarily binds at TAD borders, there also exist putative CTCF-binding sites within TADs, which are spread throughout the genome by retrotransposition. However, the detailed mechanism responsible for masking the putative CTCF-binding sites remains largely elusive. Here, we show that the ATP-dependent chromatin remodeler, chromodomain helicase DNA-binding 4 (CHD4), regulates chromatin accessibility to conceal aberrant CTCF-binding sites embedded in H3K9me3-enriched heterochromatic B2 short interspersed nuclear elements (SINEs) in mouse embryonic stem cells (mESCs). Upon CHD4 depletion, these aberrant CTCF-binding sites become accessible and aberrant CTCF recruitment occurs within TADs, resulting in disorganization of local TADs. RNA-binding intrinsically disordered domains (IDRs) of CHD4 are required to prevent this aberrant CTCF binding, and CHD4 is critical for the repression of B2 SINE transcripts. These results collectively reveal that a CHD4-mediated mechanism ensures appropriate CTCF binding and associated TAD organization in mESCs.

• 대한치과교정학회지
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• 제53권2호
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• pp.125-136
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• 2023

Before progress was recently made in the application of temporary anchorage devices (TADs) in bio-mechanical design, orthodontists were rarely able to intrude molars to reduce upper posterior dental height (UPDH). However, TADs are now widely used to intrude molars to flatten the occlusal plane or induce counterclockwise rotation of the mandible. Previous studies involving clinical or animal histological evaluation on changes in periodontal conditions after molar intrusion have been reported, however, studies involving human histology are scarce. This case was a Class I malocclusion with a high mandibular plane angle. Upper molar intrusion with TADs was performed to reduce UPDH, which led to counterclockwise rotation of the mandible. After 5 months of upper molar intrusion, shortened clinical crowns were noticed, which caused difficulties in oral hygiene and hindered orthodontic tooth movement. The mid-treatment cone-beam computed tomography revealed redundant bone physically interfering with buccal attachment and osseous resective surgeries were followed. During the surgeries, bilateral mini screws were removed and bulging alveolar bone and gingiva were harvested for biopsy. Histological examination revealed bacterial colonies at the bottom of the sulcus. Infiltration of chronic inflammatory cells underneath the non-keratinized sulcular epithelium was noted, with abundant capillaries being filled with red blood cells. Proximal alveolar bone facing the bottom of the gingival sulcus exhibited active bone remodeling and woven bone formation with plump osteocytes in the lacunae. On the other hand, buccal alveolar bone exhibited lamination, indicating slow bone turnover in the lateral region.

• 한국군사과학기술학회지
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• 제17권6호
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• pp.786-792
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• 2014

In this paper, we propose a novel method which can be applied to test and verify the stabilization-tracking performance of Gun/Turret Driving System(GTDS) for Light Armed Helicopter(LAH). In real system, GTDS is connected to TADS/SMC and drived to aim at the target with 20mm gun. But each equipment is separately developed during exploratory development stage, so GTDS cannot be tested under real system state. We suggest new configuration of test system for evaluating the stabilization-tacking performance, in which TADS and SMC are replaced by vision acquisition unit and processing unit, respectively.

• 구강회복응용과학지
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• 제30권4호
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• pp.265-277
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• 2014

목적: 고정식 교정장치에서 removable sliding jig (R-jig)와 temporary anchorage devices (TADs)를 이용하여 상악 구치의 원심 이동 시 힘의 크기와 와이어의 단면 크기에 따른 치아 이동과 치조골에 미치는 효과의 차이를 유한요소방법을 통하여 분석하고자 한다. 연구 재료 및 방법: 정상적인 치아 크기와 악궁 형태를 가진 상악 치아와 상악 치조골을 3차원 형상 모델링하고, 브라켓, 교정용 호선 및 R-jig를 포함한 유한요소 모델을 제작하였다. 골격성 고정원인 mini-implant를 제2소구치와 제1대구치 사이 협측으로 주호선 기준 8 mm 상방 위치시키고, mini-implant를 직접 고정원으로 사용하여 R-jig에 후방력을 부여하였다. 주호선을 $0.019{\times}0.025$ inch SS로 설정하고, R-jig의 와이어 단면 크기를 $0.019{\times}0.025$ inch SS, 후방력의 크기를 200 gm, 결찰을 하지 않는 조건을 기준 조건으로 설정하였다. 그리고 R-jig의 와이어 단면 크기, 후방력의 크기를 다르게 한 조건들을 설정하여 비교하였다. 결과: R-jig의 와이어 단면 크기가 증가함에 따라 후방력에 의한 R-jig의 변형이 적게 관찰되었지만, 제2대구치의 변위량은 아주 미미한 차이를 보였다. 제2대구치에 가해지는 후방력의 크기가 증가할수록 변위량도 같이 증가하였고, 원심경사 경향과 수직 변위량도 더 커졌다. 결론: R-jig는 임상적으로 큰 부작용 없이 치아의 원심이동을 가능하게 한다.