• 제목/요약/키워드: TA-4

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뇌하수체 호르몬과 포르볼에스터에 의한 참개구리 난자의 배란과 프로스타글라딘 합성유도 (Induction of Oocyte Ovulation and Prostaglandin Synthesis by Gonadotropin and Phorbol Ester in vitro in Amphibian (Rana n igromacu la ta) Ovarian Follicles)

  • 장경자;나철호;소재목;이원교;권혁방
    • 한국동물학회지
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    • 제39권3호
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    • pp.266-272
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    • 1996
  • 봄에 채집한 참개구리의 난소조각 배양계를 사용하여 난자의 배란과정에 프로스타글란딘과 protein kinase C(PKC)가 관여하는 지를 조사하였다. 난소조각을 배양하면서 뇌하수체추출물(FPH) 혹은PKC의 활성제인 12-O-tetradecanoyl phorbol-13-acetate (TPA)를 처리한 후 배란율과 프로스타글란딘의 생상량을 방사면역측정법으로 조사한 결과 농도에 의존하여 난자의 배란이 유도 되었으며 프로스타글란딘의 생성이 촉진되었다. FPH와 TPA에 의한 배란과 프로스타글란딘 생성은, 4월 보다는 5월에 채집한 개구리에서 훨씬 더 효과적이었다. FPH처리는 프로스타글란딘과 함께 progestreone의 생성을 촉진하였으나 TPA는 progestreone의 생성을 촉진하지는 못하였다. FPH와 TPA에 의한 배란과ㅣ 프로스타글라딘 생성 억제제인 indomethacin에 의해서는 난자의 배랑니 억제되지는 않았다. 이러한 결과들은 참개구리 난자의 배란 과정에 PKC의 활성화가 중요한 역할을 하며, 프로스타글라딘의 생성이 매개할 것으로 생각된다.

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진주시 대기중 휘발성 유기화합물의 농도특성 기초조사 (Characterization of Volatile Organic Compounds(VOCs) Concentrations in Jinju)

  • 박정호;박현건;서정민
    • 한국환경과학회지
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    • 제22권1호
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    • pp.91-98
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    • 2013
  • In order to study the seasonal patterns and possible origins of air concentrations of volatile organic compounds(VOC), measurements were taken with GC-MS at 3 sampling sites in Jinju for 12 months from Mar. 2010 to Feb. 2011. Atmospheric VOC are sampled on tubes containing solid adsorbents(Tenax TA) with a time resolution of 2hrs. Composition and concentration of VOC are analysed with a GC system equipped with thermal desorption apparatus(ATD). The most abundant compound appeared to be Toluene, Ethylbenzene and m,p-Xylene. The mean concentrations of Benzene were 0.20 ppb at GN site, 0.18 ppb at DA site, and 0.25 ppb at SP site, respectively. VOC concentration showed a strong seasonal variation, with higher concentrations during the spring and lower concentrations during the summer. The results showed that monthly fluctuations in measured VOC concentrations depended on variations in the strength of sources, as well as on photochemical activity and meteorological conditions. In Jinju, the total VOC emissions for 2009 were estimated to be 4,407 ton/year by Clean Air Policy Support System(CAPSS). It is shown that solvent use 57.5%(2,534 ton/yr), waste treatment and disposal 23.3%(1,025 ton/yr), and mobil source-road traffic 12.2%(537 ton/yr) are the most significant anthropogenic source.

부적응 병사들에 대한 교류분석 집단상담 프로그램의 효과 (Effect of Transactional Analysis Group Counselling Program for Maladjusted Soldiers)

  • 박신영;이은미
    • 한국콘텐츠학회논문지
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    • 제17권3호
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    • pp.633-642
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    • 2017
  • 본 연구는 교류분석 프로그램이 육군 부적응 병사들의 개인역량과 의사소통 능력에 미치는 효과를 파악하기 위한 실험연구로 단일군 전 후 설계이다. 연구대상자는 G시에 소재한 ${\bigcirc}{\bigcirc}$부대 ${\bigcirc}{\bigcirc}$캠프에 참가하고 있는 군 복무부적응 병사 8명이었고, 자료수집기간은 2015년 10월 15일에서 10월 21일까지였다. 교류분석 프로그램은 1주일에 2회, 1회당 240분(2회기)으로 2주 동안 총 8회기로 진행하였으며, 자료 수집은 실험군에게 사전-사후 자기기입식 설문방법으로 진행하였다. 자료 분석방법은 Wilcoxon의 부호순위 검정을 이용하였고, 연구결과에서 실험군은 사전에 비해 사후에 개인역량(Z=-2.366, P=.018). 개인역량의 하위요인 중 자기효능감(Z=-2.047, P=.041), 긍정성(Z=-2.058, P=.040)이 유의하게 증가하였다. 개인역량 하위요인 중 대인관계(Z=-1.179, P=.238), 군 입대 동기의 자발성(Z=-1.518, P=.129)과 의사소통 능력은 평균점수가 높아지긴 하였으나 통계적으로 유의한 차이는 없었다(Z=-1.524, P=.128). 결론적으로 본 연구에서 사용된 교류분석 집단상담이 개인역량, 자기효능감, 긍정성을 향상시켜 군 적응을 돕는데 효과적임을 시사한다.

Aflatoxin B1 Detoxification by Aspergillus oryzae from Meju, a Traditional Korean Fermented Soybean Starter

  • Lee, Kyu Ri;Yang, Sun Min;Cho, Sung Min;Kim, Myunghee;Hong, Sung-Yong;Chung, Soo Hyun
    • Journal of Microbiology and Biotechnology
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    • 제27권1호
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    • pp.57-66
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    • 2017
  • Aflatoxins are classified as Group 1 (carcinogenic to humans) by the International Agency for Research on Cancer. In this study, a total of 134 fungal strains were isolated from 65 meju samples, and two fungal isolates were selected as potential aflatoxin $B_1$ ($AFB_1$)-biodetoxification fungi. These fungi were identified as Aspergillus oryzae MAO103 and A. oryzae MAO104 by sequencing the beta-tubulin gene. The two A. oryzae strains were able to degrade more than 90% of $AFB_1$ (initial concentration: $40{\mu}g/l$) in a culture broth in 14 days. The mutagenic effects of $AFB_1$ treated with A. oryzae MAO103 and MAO104 significantly decreased to 5.7% and 6.4%, respectively, in the frame-shift mutation of Ames tests using Salmonella typhimurium TA98. The base-substituting mutagenicity of $AFB_1$ was also decreased by the two fungi. Moreover, $AFB_1$ production by Aspergillus flavus was significantly decreased by the two A. oryzae strains on soybean-based agar plates. Our data suggest that the two $AFB_1$-detoxifying A. oryzae strains have potential application to control $AFB_1$ in foods and feeds.

농업경영체 등록정보를 활용한 밭 경작지의 공간적 파편화 특성 분석 (Spatial Fragmentation Analysis of Upland Fields Using Farm Manager Registration Information)

  • 이지민;유승환;오윤경
    • 한국농공학회논문집
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    • 제60권2호
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    • pp.13-24
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    • 2018
  • As food consumption pattern changes (reduced rice consumption and increased consumption of fresh vegetables), managing upland fields became a greater priority. However, the agricultural infrastructure projects have been focused on rice farming, and the infrastructure level to support dry fields cultivation is insufficient. The purpose of this study was to spatial distribution analysis of these dry fields with farm manager registration information. Using FRAGSTATS, we analyzed landscape indices (TA/CA, NP, PD, LSI, LPI, PLADJ, COHESION, CONNECT, AI) of farmlands in which farmers in 13 regions (Si or Gun) cultivated dry-field crops. As results of this analysis, the total area of the fields in Naju-si, Hampyeong-gun and Suncheon-si were found to be wider, but the average area of a patch in Youngam-gun, Hampyeong-gun and Jangseong-gun were wider than other regions. On average, each farmer had farmlands containing of 1.7~2.4 patches and cultivated crops in fields of 0.2~0.5 ha. Farmlands of Hampyeong-gun, Youngam-gun, Naju-si showed high values in adjacency indices, however the farmlands of Suncheon and Gwangyang showed fragmented distribution with low values in adjacency indices. These results of fragmentation analysis of farmlands could be used when we establish the plan of an agricultural infrastructure project or select places for a collaborative agricultural management business promotion project.

Effects of Cold Stress on Broiler Performance and Ascites Susceptibility

  • Ipek, A.;Sahan, U.
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권5호
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    • pp.734-738
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    • 2006
  • The present study was conducted to determine the effect of cold stress on broiler performance and ascites susceptibility. Male chicks were obtained from a commercial strain of broiler breeders. The trial was divided into two treatments (control and cold stress groups). Ascites was induced in broiler chickens in the trial by exposing the chickens to low temperature (Ta) and by supplying a pelleted diet. The two experimental treatments consisted of: 1) Control group, $33.3^{\circ}C$ the $1^{st}$ wk, $30.2^{\circ}C$ the $2^{nd}$ wk, and $27.5^{\circ}C$ the $3^{rd}$ wk. 2) Cold stress group, $29.0^{\circ}C$ the $1^{st}$ wk, $26.4^{\circ}C$ the $2^{nd}$ wk, and $23.1^{\circ}C$ the $3^{rd}$ wk. From the end of the $3^{rd}$ wk all broilers were reared to 6 wk of age at a constant temperature of $21^{\circ}C$. There was significant difference in live BW during wk 1 to 5. The control group was consistently the heaviest; however, at 6 wk of age, both groups weighed the same. Body weight gain up to 3 wk was significantly decreased by cold stress. During wk 3 and 6 the chicks in the cold stress group had greater BW gain compared with the chicks in the control group. There were significant differences in mortality due to ascites between the groups. During wk 3 and 6 the cold stress group exhibited the most ascites mortality (9.52%) when compared with the control group (1.90%). At 5 wk of age cold stress condition caused significant changes in packed cell volume (PCV), hemoglobin (Hb) and red blood cell counts (RBC). Right ventricle weight was significantly heavier in the cold stress group than the control. There were also significant differences in right ventricle/total ventricle (RV/TV) ratios at 5 wk. the right ventricle/total ventricle ratios in the cold stress group was higher (0.25) than the control group (0.20). It was concluded that, fast growth and cold temperatures are the primary triggers for ascites during commercial broiler production.

Chemopreventive Activity of Turmeric Essential Oil and Possible Mechanisms of Action

  • Liju, Vijayasteltar Belsamma;Jeena, Kottarapat;Kuttan, Ramadasan
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권16호
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    • pp.6575-6580
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    • 2014
  • This study aimed to evaluate the antimutagenic and anticarcinogenic activity of turmeric essential oil as well as to establish biochemical mechanisms of action. Antimutagenicity testing was accomplished using strains and known mutagens with and without microsomal activation. Anticarcinogenic activity was assessed by topical application of 7, 12 - dimethylbenz[a]anthracene (DMBA) as initiator and 1% croton oil as promoter for the induction of skin papillomas in mice. Inhibition of p450 enzymes by TEO was studied using various resorufins and aminopyrene as substrate. Turmeric essential oil (TEO) showed significant antimutagenic activity (p<0.001) against direct acting mutagens such as sodium azide ($NaN_3$), 4-nitro-O-phenylenediamine (NPD) and N-methyl-N-nitro N'nitrosoguanine (MNNG). TEO was found to have significant antimutagenic effect (>90%) against mutagen needing metabolic activation such as 2-acetamidoflourene (2-AAF). The study also revealed that TEO significantly inhibited (p<0.001) the mutagenicity induced by tobacco extract to Salmonella TA 102 strain. DMBA and croton oil induced papilloma development in mice was found to be delayed and prevented significantly by TEO application. Moreover TEO significantly (P<0.001) inhibited isoforms of cytochrome p450 (CYP1A1, CYP1A2, CYP2B1/2, CYP2A, CYP2B and CYP3A) enzymes in vitro, which are involved in the activation of carcinogens. Results indicated that TEO is antimutagenic and anticarcinogenic and inhibition of enzymes (p450) involved in the activation of carcinogen is one of its mechanisms of action.

바닥재로부터 방출되는 휘발성유기화합물과 폼알데하이드 특성 (Characteristics of VOCs and Formaldehyde Emitted from Floorings)

  • 박현주;장성기;서수연;임준호
    • 한국대기환경학회지
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    • 제25권1호
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    • pp.38-45
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    • 2009
  • Since the seventies and the oil crisis, energy-saving measures have led to a reduction in the ventilation of room. The use of synthetic materials which emit various chemical substances had led to an increase in the concentration of indoor pollutants. "Sick building syndrome (SBS)" and "Sick house syndrome (SHS)" are worldwide problems. Also, the number of complaints about indoor air pollution caused by VOCs (Volatile organic compound) and HCHO (Formaldehyde) has increased. It is important that evaluating and understanding emission of indoor air pollutant from building materials. The object of this study was to evaluate emission test method for flooring such as wood based flooring, carpet tile, rubber tile, PVC sheet and tile, and to determine emission of TVOC and form-aldehyde. The quantity of TVOC and carbonyl compounds emission were sampled and measured by Tenax TA and gas chromatography/mass spectrometry (GC/MSD), 2,4-DNPH cartrige with ozone scrubber and high performance liquid from flooring. The TVOC concentration emitted from carpet tile was ($7.419\;mg/m^2 h$) the highest among 5 groups of test materials. In wood based flooring and PVC tile, the emitted concentration of toluene was high. And the dodecane emission was highest in carpet. The concentration of TVOC decreased by an increase in emission test period. After 7 days, the concentration of TVOC from floorings were about 50% below of the concentration at the first day. TVOC emission from wood based flooring, carpet tile, rubber tile, PVC sheet and tile were decreased in 28 days and remained steady after about 15 days. The concentration of formaldehyde emission from floorings showed extremely low.

ENHANCING THE PRECISION OF GPS STATIC RELATIVE POSITIONING USING THE OCEAN TIDE LOADING CORRECTION

  • Yeh, Ta-Kang;Chang, Ming-Han;Liou, Yuei-An;Chen, Chun-Sung
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume II
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    • pp.756-759
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    • 2006
  • The ocean tide loading (OTL) is an important factor for the GPS positioning, especially in the height direction. The shorter of the distance to the ocean, the larger of the error by the OTL. The influence will be changed when we measure in different place and the order of magnitude is from few centimeters to ten centimeters. In this study, more than ten kinds of the OTL models were collected and applied on the GPS static relative positioning in Taiwan. The GPS observations including five stations were obtained from Nov. 9, 2004 to Feb. 23, 2005 and we used the Bernese GPS software to execute the data processing. In this period, the average amplitudes of the 3-D coordinates are as follows: N is 0.4 cm, E is 0.7 cm, h is 1.8 cm at Kinmen station; N is 0.7 cm, E is 1.3 cm, h is 2.3 cm at Lanyu station; N is 0.5 cm, E is 0.7 cm, h is 2.0 cm at Matsu station; N is 0.6 cm, E is 0.6 cm, h is 2.0 cm at Penghu station and N is 0.5 cm, E is 1.2 cm, h is 1.7 cm at Hsinchu station. Moreover, we will analyze the advantage and disadvantage of every kind of the OTL models in different environments to offer some information to the GPS users and enhance the precision of the GPS positioning.

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효소 처리에 따른 야생복숭아(Prunus persica L.) 당절임액의 품질 특성 및 항산화 효과 (Quality Characteristics and Antioxidant Effect of Sugar Preserved Wild Peach (Prunus persica L.) Juice by Enzymatic Treatment)

  • 정경미;김선화;정용진;최미애
    • 한국조리학회지
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    • 제23권5호
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    • pp.25-33
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    • 2017
  • This study was to investigate the effects of enzyme on quality characteristics and antioxidant activities of wild peach (Prunus persica L.) juice. pH levels and S.S (soluble solid) values in all samples ranged from 3.86 to 4.13 and from 48.0 to $55.0^{\circ}Brix$, respectively. The TA (total acidity) values of control (not treatment enzyme) were higher than those of the others. The highest 'L', 'a' and 'b' values were observed on PWP (preserved wild peach (Prunus persica L.) juice of cellulase/pectinase (1:1, w/w) sample. Glucose (26.65 g/100 g) and fructose (17.42 g/100 g) in PWP product were determined, however sucrose and maltose were not detected. The predominating organic acid components analyzed in PWP sample were tartaric (32.36 g/100 g) and lactc acids (209.34 g/100 g), whereas citric acid, acetic acid and malic acid were not detected. Higher scores for taste, flavor, color and overall acceptance were found for PWP products compared to other samples. The total phenolic content (13.31 mg GAE/mg dry weight) analyzed using Folin-Ciocalteu's reagent, of PWP sample was higher than those of the others and the total flavonoid concentrations were also 10.95 mg CE/mg dry weight. The DPPH radical scavenging and ABTS radical scavenging activities in all samples ranged from 55.16 to 74.29% and from 39.59 to 82.79%, respectively. The antioxidant activities were affected by addition of enzyme. These results indicate that the use of the mixture of cellulase and pectinase could increase the quality, and antioxidant potentials of sugar preserved wild peach (Prunus persica L.) juice by enzymatic treatment.