• The Korean Journal of Physiology and Pharmacology
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• v.15 no.2
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• pp.101-106
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• 2011

We demonstrate that glycoprotein isolated from Dioscorea batatas (GDB) has immunostimulatory effects including macrophage activation. Analysis of infiltration of inflammatory cells into peritoneal cavity showed GDB treatment significantly increased the recruitment of macrophages, lymphocytes, neutrophils, and monocytes into the peritoneal cavity. Treatment of spleen cells isolated from C57BL/6 mice with GDB significantly increased the proliferation of B cells and T cells induced by LPS and ConA, respectively. Treatment with GDB significantly increased the cytolytic capacity of NK cells and macrophages against YAC-1 and B16 cells, respectively. In order to further confirm and investigate the mechanism of GDB on macrophage activation, we analyzed the effects of GDB on the cytokine expression including iNOS, IL-1${\beta}$, and TNF-${\alpha}$ in mouse macrophage cell line, RAW 264.7 cells. RT-PCR and ELISA showed that GDB increased the expression of IL-1${\beta}$, and TNF-${\alpha}$, whereas iNOS was not induced by GDB. Collectively, this series of experiments indicates that GDB stimulates immune system including macrophage activation.

• Journal of Periodontal and Implant Science
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• v.53 no.1
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• pp.20-37
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• 2023

Purpose: Our pilot study showed that a 3-dimensional dual drug delivery scaffold (DDDS) loaded with Chinese herbs significantly increased the regenerated bone volume fraction. This study aimed to confirm the synergistic anti-inflammatory and osteogenic preclinical effects of this system. Methods: The targets and pathways of parthenolide and naringin were predicted. Three cell models were used to assess the anti-inflammatory effects of parthenolide and the osteogenic effects of naringin. First, the distance between the cementoenamel junction and alveolar bone crest (CEJ-ABC) and the bone mineral density (BMD) of surgical defects were measured in a rat model of periodontitis with periodontal fenestration defects. Additionally, the mRNA expression levels of matrix metallopeptidase 9 (MMP9) and alkaline phosphatase (ALP) were measured. Furthermore, the number of inflammatory cells and osteoclasts, as well as the protein expression levels of tumor necrosis factor-alpha (TNF-α) and levels of ALP were determined. Results: Target prediction suggested prostaglandin peroxidase synthase (PTGS2) as a potential target of parthenolide, while cytochrome P450 family 19 subfamily A1 (CYP19A1) and taste 2 receptor member 31 (TAS2R31) were potential targets of naringin. Parthenolide mainly targeted inflammation-related pathways, while naringin participated in steroid hormone synthesis and taste transduction. In vitro experiments revealed significant antiinflammatory effects of parthenolide on RAW264.7 cells, and significant osteogenic effects of naringin on bone marrow mesenchymal stem cells and MC3T3-E1 cells. DDDS loaded with parthenolide and naringin decreased the CEJ-ABC distance and increased BMD and ALP levels in a time-dependent manner. Inflammation was significantly alleviated after 14 days of DDDS treatment. Additionally, after 56 days, the DDDS group exhibited the highest BMD and ALP levels. Conclusions: DDDS loaded with parthenolide and naringin in a rat model achieved significant synergistic anti-inflammatory and osteogenic effects, providing powerful preclinical evidence.

• Journal of the Korean Society of Costume
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• v.16
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• pp.111-123
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• 1991

We suppose that Poback-cheok appeared with wearing clothes in the ancient times. At first, man used body as a scale, and it is gradually diversified with social development. It is clear that also in the time of Shilla-Dynasty, the United Shilla-Dynasty and the Goryeo-Dynasty, the system of degree existed in historical relation to that of China. But we don't know it's real length, because there is no recording until the beginning of the Yi-Dynasty. The first recording about Poback-cheok appeared in the time of the King Sejong. Hwangjong-Cheok which is a original vessel of the degree system appears in the 7th years of the King Sejong. In the 12th years of the King Sejong, Ju-cheok, Youngjo-cheok and Joyegi-cheok appeared. In the 13th years of the King Sejong, Poback-cheok appeared as a new changed one. At that time, the length of Poback-cheok is recorded as 44.75cm in chapter of Oye and Jonsoo Pobaek-cheok is recorded as 46.73cm in Jeonjesangjeongso's picture, and it is recorded as 46.80cm in the Kyunggugdaijeon. The scale system is put in good order in the time of King Sejong. But it is confused by the war of 1636 after King Seonjo period. In the 26 th year of King Youngjo, it is reappeared as one of the King Sejong, and it is fixed as 46.80cm. The length is used until the proclamation in the 6th years of the Kwangmu(1902), and then the length of Pobaek-cheok is changed as 48.48 cm, that is measured by a Gok-cheok. After that time, the scale system is very confused during the introduction of new civilization and the Korean-Japanes Unit. So it is used longer or shorter(that is, 49.24cm or 48.91cm). And the metric system is introduced by Japanes in 1926, and it reached the present time. According to use, the actual thing with is used in the end of the Yi-Dynasty is various from 30cm to 70cm. And according to material and craft-man, it can be devided into Hwa-gag scale, Najeon-blacklacquer-scale, bamboo-scale, wooden-scale. The way of expression of measure is always followed by the decimal system. The other half part, except the part of expression of graduation, is put on decorate with a pattern. Also we can see the idea of living on this point.

• Journal of Microbiology and Biotechnology
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• v.6 no.2
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• pp.79-85
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• 1996

By using a T7 expression system, a large amount of Bacillus stearothermophilus endo-xylanase A (XynA) could be produced in Escherichia coli cells. The overproduced enzyme formed inclusion bodies, and so the protein could be more easily purified to homogeneity. The molecular weight of the purified enzyme was estimated to be 22 kDa by SDS-polyacrylamide gel electrophoresis and 43 kDa by Sephacryl S-200 gel filtration, suggesting that the native enzyme was a homodimer. The pI value was determined to be 8.4. The Michaelis constants for birchwood xylan and oat spelts xylan were calculated to be 3.83 mg/ml and 5.03 mg/ml, respectively, and the $V_{max}$ max/ values for both xylans were 2.86 $\mu mole$/min. The purified enzyme was most active at $55^{\circ}C$ and pH 8.0, and stable up to $60^{\circ}C$ and in the near neutral pH range. From the zymogram, Bacillus stearothermophilus was found to have at least three xylanases and the purified one was the smallest among them.

• Journal of Families and Better Life
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• v.20 no.3
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• pp.73-85
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• 2002

The purpose of this study was to develop and evaluate the Marital Equality Education Program for women farmers and their husband. Based on the empirical research result of 980 women farmers as basic study for developing this program, marital equality theory, system theory, strong family perspective, stress coping theory, anger control theory, modified Couple Communication Program, marriage enrichment methods, 7 session program was developed. The contents of every session were Reviewing Marriage Relationships, Coping Role Stress, Marital Equality, Self Expression & Speaking, Understanding Spouse & Listening, Anger Control& Resolving Conflict, and Enhancing Marital Relations. The effectiveness of this program was evaluated by pretest, post test, and follow-up 1 month later through 35 rural couples(women farmers and their husband) and it was analyzed by paired t-test and qualitative evaluation was also investigated. The major results were as follows : This program was effective in improving their self-esteem, marital satisfaction, coping role stress & conflicts, and husband's egalitarian role attitude & practice. The results suggested the program had also Positive impacts on the marital equality and relationships. Future research and practical implications were added.

• Journal of Microbiology
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• v.42 no.3
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• pp.205-210
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• 2004

The sequence coding for carboxymethylcellulase (CMCase, CelC) was isolated from the DNA of Salmonella typhimurium URl. Comparison between the deduced amino acid sequence of CelC (368 amino acid residues, Molecular mass 41 kDa) and that of the previously published CMCase revealed that this enzyme belongs to the cellulase family 8 and D. The protein was overproduced in Escherichia coli using T7 expression system, and its activity was confirmed by CMC-SDS-PAGE. When the overexpressed CelC protein was tested on cellulose-type substrates, the recombinant protein is able to degrade cellulose-type substrates, such as CM-cellulose, xylan, avicel, lichenan, and laminarin. Optimal temperature and pH for enzyme activity were found to be 50$^{\circ}C$ and pH 6.5, respectively.

• Journal of Korean Biological Nursing Science
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• v.7 no.1
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• pp.47-56
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• 2005

nm23-H1 gene expression has been inversely correlated with tumor metastatic potential in certain tumors including melanomas, breast carcinomas, and hepatocellular carcinomas. However, its role with respect to the invasive behavior of central nervous system tumors has scarcely been addressed Because cell motility and invasion plays an essential role in metastatic dissemination, we have studied whether motile human glioma cell(U87MG) transfected with nm23-H1 complementary DNA have any alterations in their ability to migrate and invade. There was no significant changes in the shape and size of the cells following nm23-H1 transfection. The role of nm23-H1 in glioma migration and invasion have been evaluated by in vitro simple scratch technique and brain slice invasion model Basal migration ability of nm23-H1 transfectants cell(U87MG-pEGFP-nm23) were lesser than U87MG. Accordingly, U87MG-pEGFP-nm23 didn't migrate away apparently from the tumors implanted site comparing U87MG in brain slice invasion model. These results suggest that nm23-H1 may play an important role in suppressing the human glioma migration and invasion.

• Development and Reproduction
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• v.21 no.1
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• pp.93-100
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• 2017

Obesity is characterized by a state of chronic low-grade inflammation and insulin resistance, which are aggravated by the interaction between hypertrophic adipocytes and macrophages. In this study, we investigated the effects of tangeretin on inflammatory changes and glucose uptake in a coculture of hypertrophic adipocytes and macrophages. Tangeretin decreased nitric oxide production and the expression of interleukin (IL)-6, $IL-1{\beta}$, tumor necrosis $factor-{\alpha}$, inducible nitric oxide synthase, and cyclooxygenase-2 in a coculture of 3T3-L1 adipocytes and RAW 264.7 cells. Tangeretin also increased glucose uptake in the coculture system, but did not affect the phosphorylation of insulin receptor substrate (IRS) and Akt. These results suggest that tangeretin improves insulin resistance by attenuating obesity-induced inflammation in adipose tissue.

• Journal of the Korea Computer Industry Society
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• v.2 no.7
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• pp.953-966
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• 2001

It has been widely recognized that our educational system hasn't been able to keep up with the fast advancement of technological development. We can frequently meet the expression： students of twenty-first century and schools and teachers of nineteenth century. All the people in this country arouse their interest in education and exclaim as experts. However, there's no one to believe that the condition of education is improved. Currently, as internet is being delved into all parts of society, cyber education is being spread with rapid speed. I want to focus on some of the issues raised during the last two years from some of the institutions built on the internet using this new technology. This study will find the limits of cyber education with virtual class and suggest devices to overcome the faults.

• Journal of Microbiology and Biotechnology
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• v.29 no.6
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• pp.999-1007
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• 2019

Middle East respiratory syndrome coronavirus (MERS-CoV) was first identified in Saudi Arabia in 2012 and related infection cases have been reported in over 20 countries. Roughly 10,000 human cases have so far been reported in total with fatality rates at up to 40%. The majority of cases have occurred in Saudi Arabia with mostly sporadic outbreaks outside the country except for the one in South Korea in 2015. The Korean MERS-CoV strain was isolated from the second Korean patient and its genome was fully sequenced and deposited. To develop virus-specific protective and therapeutic agents against the Korean isolate and to investigate molecular determinants of virus-host interactions, it is of paramount importance to generate its full-length cDNA. Here we report that two full-length cDNAs from a Korean patient-isolated MERS-CoV strain were generated by a combination of conventional cloning techniques and efficient Gibson assembly reactions. The full-length cDNAs were validated by restriction analysis and their sequence was verified by Sanger method. The resulting cDNA was efficiently transcribed in vitro and the T7 promoter-driven expression was robust. The resulting reverse genetic system will add to the published list of MERS-CoV cDNAs and facilitate the development of Korean isolate-specific antiviral measures.