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Effect of Total Mixed Ration with Fermented Feed on Ruminal In vitro Fermentation, Growth Performance and Blood Characteristics of Hanwoo Steers

  • Kim, S.H.;Alam, M.J.;Gu, M.J.;Park, K.W.;Jeon, C.O.;Ha, Jong-K.;Cho, K.K.;Lee, S.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.2
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    • pp.213-223
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    • 2012
  • In this study, two experiments were conducted to evaluate the total mixed ration with fermented feed (TMRF) and total mixed ration (TMR) by rumen in vitro fermentation and their effects on the growth performance and blood characteristics of Hanwoo steers. In experiment 1, three Hanwoo steers ($600{\pm}47$ kg), each permanently fitted with a ruminal cannula were used. In this experiment, three diets designated as T1, TMRF (18.4% fermented feed, tall fescue, mammoth wild rye forage and whole crop barley); T2, TMRF (17.7% fermented feed, rice straw and whole crop barley); and T3, TMR (rice straw, whole crop barley and probiotics, but no fermented feed), which were subjected to rumen in vitro fermentation for 48 h. The results demonstrated that DM disappearance rate gradually increased with advancing fermentation time, but T1 and T2 were higher than the T3 (p<0.05) from 3 h to 12 h, but insignificant (p>0.05) at 24 and 48 h. None of the specific VFAs were affected except for acetic and non volatile lactic acids, which were produced more in T2 than in T1 and T3 at 24 h and 48 h of incubation. A/P was lower in T1 and T2 than inT3 at 24 h (p<0.05) and 48 h (p>0.05) of incubation. These results confirmed that TMRF-related treatment shows a superior performance to that of TMR during the ruminal fermentation period. In experiment 2, the three diets in experiment 1 plus 1 more control diet (concentrates, probiotics and 2% rice straw of body weight) were fed to the 48 Hanwoo steers ($160{\pm}10$ kg) for a period of 168 d. The results demonstrated that the daily and total live weight gain and feed efficiency were higher (p<0.05) in the TMRF and TMR groups than in the control group. SGOT, SGPT and BUN (p<0.05) were reduced in TMRF relative to the control and TMR groups by 168 d which confirmed that TMRF shows better blood profiles than the TMR and control groups. Overall, these results appear to show that TMRF has better in vitro ruminal characteristics than those of TMR; growth performance and blood profiles were also found to be superior in TMRF than in the TMR and control groups. Thus, our findings suggest that TMRF-based feed supplies are favorable for Hanwoo cattle.

Methylenetetrahydrofolate Reductase C677T Polymorphism in Gastric Cancer (위암에서 Methylenetetrahydrofolate Reductase C677T의 유전자 다형성)

  • Seo Won;Park Won Cheol;Lee Jeong Kyun;Kim Jeong Jung
    • Journal of Gastric Cancer
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    • v.5 no.1
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    • pp.10-15
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    • 2005
  • Purpose: Recently the role of vitamins, folate in particular, has been emphasized in the maintenance of health. Folate deficiency is known to give rise to developmental delay, immature vascular disease, neural tube defect, acute leukemia, atherosclerotic vascular disease, delivery defects, breast cancer, and particularly gastrointestinal neoplasia. Methylenetetrahydrofolate reductase (MTHFR) is an essential enzyme in folate metaboism, and influences DNA synthesis and DNA methylation. Generally, folate deficiency is associated with gastrointestinal neoplasms. The amino-acid- changing and enzyme-activity-reducing nucleotide polymorphism (766C$\rightarrow$T/ Ala222Val) has been described in the MTHFR polymorphism and leads to low enzyme activity that may reduce the capacity of DNA methylation and possibly uracil mis-incorporation into DNA. These processes may be critical in the oncogenic transformation of human cells, especially in colorectal carcinomas. We investigated the relationship between the MTHFR polymorphism in gastric cancer and the tumor site, the smoking history, and the alcoholic history. Materials and Methods: Ninety-six (96) individuals with gastric cancer and 287 healthy persons were analyzed. Blood sampling was performed, PCR-RFLP was analyzed, and MTHFR polymorphism genotypes of C/C, C/T, and T/T were obtained and analyzed statistically for their correlation. Results: In the gastric cancer group there were 69 ($72\%$) males and 27 ($28\%$) females. There were also 58 cases ($60\%$) involving the gastric lower body, 20 cases ($21\%$) the gastric mid-body, and 18 cases ($19\%$) the gastric upper body. In the control group there were 169 ($59\%$) males and 118 ($41\%$) females. Among the gastric cancer, 56 ($61\%$) smoking patients, 40 ($39\%$) non-smoking patients, 45($47\%$) alcoholic patients, 51 ($53\%$) non-alcoholic patients. In the gastric cancer group, MTHER polymorphisms were C/C in 18 ($19\%$) cases, C/T in 59 ($61\%$) cases, T/T in 19 ($20\%$) cases. In the control group polymorphisms were C/C 116 ($40\%$) cases, C/T 103 ($36\%$) cases, and T/T 68 ($24\%$) cases (P=0.045). In cases of lower gastric body cancer, polymorphisms were C/C in 16 ($24\%$) C/C in 16 ($24\%$) cases and C/T or T/T in 42 ($72\%$) cases. In cases of upper and mid-body cancer, polymorphisms were C/C in 2 ($5\%$) cases and C/T or T/T 36 ($95\%$) cases (P=0.006). In the non-smoking patient group, polymorphisms were C/C in 5 (12%) cases and C/T or T/T in 35 ($88\%$) cases. In the smoking patient group, C/C in 13 ($23\%$) cases and C/T or T/T in 43 ($77\%$) cases (P=0.189). In the non-alcoholic patient group, polymorphisms were C/C in 6 ($12\%$) cases and C/T or T/T in 45 ($88\%$) cases. In the alcoholic patient group, polymorphisms were C/C in 12 ($26\%$) cases and C/T or T/T in 33 ($74\%$) cases (P=0.063) Conclusion: MTHFR polymorphisms are associated with gastric cancer and tumor site, but not with smoking and alcohol drinking.

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A Study on Live Load Design Standards Considering Moving Load (For Shorter than 60m Span) (이동하중을 고려한 활하중 설계기준 연구 (60m 이하 교량))

  • Jin, Kyung Seok;Han, Man Yop
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.4
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    • pp.1261-1270
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    • 2013
  • The current domestic design criteria of live load employs DL-24 load and DB-24 load. Particularly for long span bridges above 45meters, DL-24 load is forced to apply and design them, since the shearing force and the moment of DL-24 load appears more dominate than those of DB-24. But it appeared that this DL-24 load didn't meet the vehicles traveling load, which affected bridges in real use. Hence this paper defined ML-24 load similar to the load applied to real bridges and also defined a new live load model, RL-24 load, after adjusting the existing DL-24 load, which doesn't meet the moment and the shearing force of ML-24. As the result of applying and reviewing RL-24 load to simple bridges of span of 45~60m, the results satisfying both the moment and the shearing force applied to bridges in real use by traveling load were attained. Besides, the applicability of it was examined in comparison with live load models of home and abroad.

Survey on Regulatory Status of Traditional and Complementary Medicine through Korean Embassies in 33 Countries (33개국 대한민국 재외공관을 통한 전통의학 및 보완대체의학 관련 제도 조사)

  • Park, Yu Lee
    • Journal of Society of Preventive Korean Medicine
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    • v.19 no.1
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    • pp.35-46
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    • 2015
  • Objective : This study aims to investigate legal and regulatory status of traditional and complementary medicine (T&CM) focusing on regulation on health practitioners and health practice in 33 countries. Method : 33 countries were selected based on several factors such as interest of Korean medical doctors, strategic importance, and distribution over the world. The questionnaire was distributed to Korean embassies in 33 countries in March 2014 through Ministry of Foreign Affairs, and the answers from those countries were collected from April to September. 24 countries that provided sufficient information were included in the analysis. Results : 18 countries have law or regulation on T&CM. Only five countries regulate T&CM practitioners as medical personnel or health practitioner by law, and 12 countries have regulation on license or certificate. Half of 24 countries recognize license of T&CM practitioners issued abroad. There are nine countries that recognize T&CM practice as medical practice, and four of them regulate acupuncture as medical practice by western medical doctors or a few health practitioners recognized by the government. There are six countries that do not recognize T&CM practice as medical practice by law, but regulate it as practice that affect public health, and these countries have law or regulation on T&CM. Conclusion : As T&CM have great impact on public health, many countries have recently legislated law or regulation on T&CM. Rapid change in regulatory status of T&CM affects globalization of Korean medicine. Thus, development of timely strategies will be essential for it.

Gender Role Identity and Egalitarianism Consciousness among Adolescents (남녀 청소년의 성역할 정체감과 양성평등의식)

  • Kim, Young-Sook;Jeong, Bok-Rae
    • Research in Community and Public Health Nursing
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    • v.18 no.2
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    • pp.310-319
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    • 2007
  • Purpose: This study intends to investigate gender role identity and egalitarianism consciousness among adolescents and to determine the relationship between the two factors. Method: Data were collected from 376 middle and high school students in U City. Tools used were the Korean Sexual Role Identity (Kim, J. H., 2005) and the Gender Egalitarianism Consciousness among Adolescents (Kim, 2002). Data were analyzed using SPSS 12.0 for t-test, one-way ANOVA and Pearson correlation coefficient. Results: There was no significant difference between boys and girls in sender role identity. Boys' gender role identity was different according to school grade (t=-3.19, p= .00), frequency of mother's hugging (t=-2.28, p= .02) and girls' according to religion (t=-2.24, p= .03) mother's job (F=3.59, p= .02), father's education level (F=3.20, p= .04), mother's education level (F=3.24, p= .04), family harmony (F=7.28, p= .00), and frequency of mother's hugging (t=-2.41. p= .02). There was significant difference in egalitarianism consciousness according to sender (t=-12.70. p= .00) Boys' egalitarianism consciousness was different according to school type (t= .02, p= .00) and father's education level (F=4.97, p= .02), and girls' according to school grade (t=-4.21 p= .00), school type (t=-3.12, p= .00), father's level of education (F=3.78. p= .02), decision maker of important domestic matter (F=4.95, p= .00) and frequency of mother's hugging (t=2.53, p= .01). In Girls, sexual role identity and sender egalitarianism consciousness were significantly correlated with each other (r= .01, p= .04). Conclusion: Through the study, it was found that boys' concern for sexual equality of was remarkably lower than girls. Therefore, it is necessary to offer curriculums for boys and to start social education for parents.

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An Empirical Research on the IoT Basis Gas AMI Platform and Smart Metering Services (IoT 기반 가스 원격검침(AMI) 플랫폼과 서비스의 실증 연구)

  • Lee, Seungwoo;Lee, Sangshin;Song, Min-hwan;Kwon, Youngmin
    • Journal of the Korean Institute of Gas
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    • v.24 no.3
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    • pp.1-10
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    • 2020
  • This paper describes the development of a smart advanced metering infrastructure(AMI) architecture and services for using smart metering in gas industry. A general gas AMI system is composed of a smart gas meter, IoT network, the AMI platform, and an operation management system with security functions. The proposed gas AMI platform supports two-way communication between smart metering devices and AMI services and is applied by oneM2M standard to support interoperability between various types of metering devices and heterogeneous IoT networks. To demonstrating AMI system with the proposed platform, we installed about 2,900 smart gas meters in real environments and operated AMI systems for one year. We verified that about 94% of gas meters are normally worked and AMI services are stably operated without error or malfunction.

Membrane-Coupled Sequencing Batch Reactor System for the Advanced Treatment of Rural Village Sewage (막결합 연속회분식 반응기를 이용한 농촌마을 하수의 고도처리)

  • Kim, Seung-Geon;Lee, Ho-Won
    • Membrane Journal
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    • v.24 no.1
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    • pp.20-30
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    • 2014
  • A membrane-coupled sequencing batch reactor (MSBR) was used for the advanced treatment of rural village sewage which is very low C/N ratio. The effect of powdered activated carbon, aeration rate, and external organic material loadings on the treatment efficiency and filtration performance were investigated in sequencing batch reactor, in which a flat-sheet type microfiltration membrane with a pore size of $0.4{\mu}m$ was submerged. At the initial operation (within 54 days) MLSS concentration, and the removal efficiencies of COD, T-N, and T-P were increased with the increase of C/N ratio. After 89 days the removal efficiencies of COD, T-N, and T-P were 97.1%, 75.0%, and 48.3%, respectively. Suspended solid-free effluent was obtained by membrane filtration. The T-P removal was relatively low because of depending on the amount of excess sludge wasting. During the operation of MSBR with powdered activated carbon, the particle size of the sludge reduced by the increase of collision frequency and mixing intensity. In comparison with MSBR without powdered activated carbon, TMP of MSBR with that was significantly elevated.

Cationic Cure of Epoxy Resin by an Optimum Concentration of N-benzylpyrazinium Hexafluoroantimonate

  • Lee, Jong-Keun;Park, Yusong;Jae-Rock lee;Park, Jaekyeung
    • Macromolecular Research
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    • v.10 no.1
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    • pp.34-39
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    • 2002
  • Cure behavior of an epoxy resin was investigated at different cure temperatures (110, 120, 130, 140, and 150 $^{\circ}C$) and cure times in the presence of 2 wt% of an N-benzylpyrazinium hexafluoroantimonate (BPH) cationic catalyst by means of differential scanning calorimetry (DSC) and dynamic mechanical analysis (DMA). The glass transition temperature ( $T_{g}$) and chemical conversion (x) at the different temperatures were determined from DSC thermograms. The $T_{g}$ and x vs. In time data were superposed up to $T_{g}$ = 10$0^{\circ}C$ and x = 0.70 by shifting horizontally at a reference temperature of $T_{g}$ = 13$0^{\circ}C$. It is interesting that the $T_{g}$ and x of the superposed data increase rather slowly in the early stage of cure and rapidly thereafter. Therefore, the increase of the $T_{g}$ and x can be divided into two regions; $R_{I}$= -18.4(= $T_{go}$ ) ~5$^{\circ}C$ and $R_{II}$ = 5 ~ 10$0^{\circ}C$ in $T_{g}$, and $R_{I}$ : 0~0.24 and $R_{II}$ : 0.24~0.70 in x. The $R_{I}$ is closely related to the initiation reactions between BPH and epoxy and between hydroxy group and epoxy in this epoxy/catalyst system. From the kinetic analysis of the $T_{g}$-shift, activation energy was 12.5 kcal/mol. The relationship between $T_{g}$ and x was also considered. The gelation and vitrification times for different cure temperatures were obtained from DMA curves.urves. DMA curves.urves.

Design and Implementation of Zigbee-CoAP-based Standby Power Saving System (Zigbee-CoAP 기반 대기전력 절감 시스템 설계 및 구현)

  • Jang, Young-Hwan;Lee, Sang-Soon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.24 no.5
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    • pp.616-622
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    • 2020
  • Recently, as the interest in power saving increases with the development of IoT technology, research on power saving of smart devices, which are being miniaturized, is being actively conducted. In particular, due to the diversification of IoT-based smart devices and the increase in personal penetration rate, the issue of standby power even when not in use is increasing. However, there is a drawback of physically unplugging the outlet or turning off the power in order to substantially reduce standby power. Since Zigbee is used instead of the existing IP, it is necessary to change to the IoT standard protocol based on the Internet network. Therefore, this paper designed and implemented the standby power saving system applying CoAP, the IoT standard protocol, to the existing Zigbee technology. Existing systems and evaluations confirmed that the average standby power of about 8.4Wh was reduced.

Physicochemical Properties of Pork Loin Marinated with a NaCl and Sodium Tripolyphosphate Solution and Sensory Attributes of Tomato Sauce-Stewed Products using Marinated Loin (염용액으로 마리네이드 처리한 돈육 등심의 이화학적 특성과 토마토 스튜 제품에서의 관능 평가)

  • Kim, Il-Suk;Jin, Sang-Keun;Park, Ki-Hoon;Jung, Gi-Jong;Kim, Dong-Hun;Lee, M.;Choi, Jine-Sang;Hoe, Soon-Ku
    • Food Science of Animal Resources
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    • v.26 no.4
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    • pp.411-417
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    • 2006
  • This study was conducted to evaluate the physicochemical properties of pork loin marinated with a solution of Nan and sodium tripolyphosphate, and the sensory attributes of tomato sauce-stewed products using marinated loin. Pork loin samples were cut $(3{\times}3{\times}2cm)$ and assigned to 3 treatment groups [C; unmarinated control (100% distilled water), T1; 10% sodium chloride, T2; 10% sodium chloride + 3% sodium tripolyphosphate]. Samples were marinated for 24hr at $5^{\circ}C$. The uptake of marinade in the treatment groups was significantly greater (p<0.05) than that of C, however no significant difference between the two treatments was observed. Cooking losses were highest (p<0.05) for T1, while lowest (p<0.05) for T2. All marinated loins had a significantly higher (p<0.05) yield than the control. The pH of the marinade solution ranged from 7.00 for T1 to 8.47 for T2. The two marinated loins had a significantly higher (p<0.05) $pH_{24}$ than the control. The water holding capacity (WHC) was highest for T2, and lowest for T1. CIE $L^*,\;a^*,\;b^*$ tended to be slightly higher in the control than either treatment group. The shear force value $(kg/cm^2)$ of yaw meat did not differ between the control and marinated muscle samples. However, cooked meat had a significantly lower (p<0.05) shear force value in T2 than C and T1. Hardness values were significantly lower (p<0.05) for both treatments compared with the control. There were no differences in texture profile, except hardness, between the control and the two treatments. Regarding the sensory evaluation of tomato sauce-stewed products manufactured with marinated pork loin, the treatment groups scored marginally well in tenderness, juiciness and overall acceptability, while the flavor score of the control was significantly higher than those of T1 and T2.