• Title/Summary/Keyword: T23

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In vitro Selection of RNA Aptamers which Bind to Escherichia coli tRNAVal (대장균 tRNAVal에 결합하는 RNA Aptamer들의 시험관내 선별)

  • Jo, Bong Rae
    • Journal of the Korean Chemical Society
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    • v.46 no.2
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    • pp.157-163
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    • 2002
  • To identify RNA motifs interacting with $tRNA^{Val}$, a SELEX(Systematic Evolution of Ligands by Exponential Enrichment) was applied. Random DNA library which contains a region of ran-domized 48-mer oligonucleotide flanked by conserved sequ ence primers was transcribed into RNA pool using T7 RNA polymerase and RNA aptamers were selected with $tRNA^{Val}$ -immobilized affinity column through 14 rounds of SELEX. Some of the resulting aptamers contained a consensus sequence similar to the sequence in the loop regions of three rRNAs; C43GAAC47 sequence of 5S rRNA, G1491AAGU1495, G1379UUCC1383 sequence of 16S rRNA and C1064UUAG1068, G2110UGUA2114, C2480GACGG2485, A2600CAGU2604 sequence of 23S rRNA. These results suggest that $tRNA^{Val}$ can interact with 5S rRNA, 16S rRNA and 23S rRNA with variety in ribosome.

Changes of IgE production, splenic helper and suppressor T lymphocytes in mice infected with Paragonimus westermani (폐흡충(Paragonimus westermani) 감염이 흰쥐의 IgE 생성 및 비장림프구 아군분포에 미치는 영향)

  • Min, Deuk-Yeong;Ryu, Jae-Suk;Sin, Myeong-Heon
    • Parasites, Hosts and Diseases
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    • v.31 no.3
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    • pp.231-238
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    • 1993
  • Effects of Paragonims westermai infection were observed in mice on the change of serum IgE level, the number of peripheral eosinophils and the distribution of Thy $1.2^{+}{\;}(CD3),{\;}L3T4^{+}{\;}(CD4),{\;}and{\;}Lyt-2^{+}$ (CD8) splenic T Iymphocytes without mitogen serum IgE increased at 3 weeks after the infection and reached a peak on week 4 and maintained high levels of IgE until the 23r6 week. Peripheral eosinophil numbers Increased at the second week and attained peak level on week 9. The frequency of $L3T4^{+}$(CD4) and $Lyt-2^{+}$ (CD8) T Iymphocytes decreased slightly until 4 weeks after the infection, but not significantly. Absolute number of $L3T4^{+}{\;}and{\;}Lyt-2^{+}$ T Iymphocytes, and the ratio of L3T4/Lyt-2 were not markedly changed over the period of observation. The frequency of Thy $1.2^{+}$ (CD3) T lymphocytes in the infected group slightly decreased until 4 weeks after the infection and showed significant reductions at the 2nd and 4th week of the infection (p < 0.05).

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Power t distribution

  • Zhao, Jun;Kim, Hyoung-Moon
    • Communications for Statistical Applications and Methods
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    • v.23 no.4
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    • pp.321-334
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    • 2016
  • In this paper, we propose power t distribution based on t distribution. We also study the properties of and inferences for power t model in order to solve the problem of real data showing both skewness and heavy tails. The comparison of skew t and power t distributions is based on density plots, skewness and kurtosis. Note that, at the given degree of freedom, the kurtosis's range of the power t model surpasses that of the skew t model at all times. We draw inferences for two parameters of the power t distribution and four parameters of the location-scale extension of power t distribution via maximum likelihood. The Fisher information matrix derived is nonsingular on the whole parametric space; in addition we obtain the profile log-likelihood functions on two parameters. The response plots for different sample sizes provide strong evidence for the estimators' existence and unicity. An application of the power t distribution suggests that the model can be very useful for real data.

The Estimation of Contribution Ratio for Sub Stream in Nam River Basin (남강유역 지류·지천별 영향도 평가)

  • Lee, Jae-Woon;Kwon, Heon-Gak;Kwak, In-Soo;Youn, Jong-Soo;Cheon, Se-Uk
    • Journal of Environmental Impact Assessment
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    • v.21 no.5
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    • pp.745-755
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    • 2012
  • The relations between tributaries and mainstream were identified with the water qualities measurements in the field. Parameters of water qualities were BOD, T-N, T-P and measurements were performed by 4 events of rainfalls for 2011. The precipitation data influenced on pollutants loads. Pollutants loads were fluctuations with the seasonal variation. Gajoacheon contributed in 18.39% of BOD, 23.79% of T-N, 15.23% of T-P and Nabulcheon contributed in 13.54% of BOD, 13.05% of T-N and 13.66% of T-P in the region from Nam River_C to Nam River_D. In case of the region from Nam River_C to Nam River_D, Yongacheon river inflowed to main stream as 23.65% of BOD, 20.74% of T-N, and 15.05% of T-P.

SOLVABILITY OF A THIRD ORDER NONLINEAR NEUTRAL DELAY DIFFERENTIAL EQUATION

  • Liu, Zeqing;Wang, Wei;Park, Jong Seo;Kang, Shin Min
    • Journal of the Chungcheong Mathematical Society
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    • v.23 no.3
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    • pp.443-452
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    • 2010
  • This work deals with the existence of uncountably many bounded positive solutions for the third order nonlinear neutral delay differential equation $$\frac{d^3}{dt^3}[x(t)+p(t)x(t-{\tau})]+f(t,x(t-{{\tau}_1}),{\ldots},x(t-{{\tau}_k}))=0,\;t{\geq}t_0$$ where ${\tau}>0$, ${\tau}_i{\in}{\mathbb{R}^+}$ for $i{\in}\{1,2,{\ldots},k\}$, $p{\in}C([t_0,+{\infty}),{\mathbb{R}^+})$ and $f{\in}C([t_0,+{\infty}){\times}{\mathbb{R}^k},{\mathbb{R}})$.

Use of 16S-23S rRNA Intergenic Spacer Region for Rapid Detection of Vibrio fluvialis (16S-23S rRNA Intergenic Spacer Region을 이용한 Vibrio fluvialis의 검출)

  • 강현실;허문수;이제희
    • Korean Journal of Environmental Biology
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    • v.21 no.1
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    • pp.77-85
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    • 2003
  • We have examined the 16S-23S rRNA intergenic spacer regions (ISR) of Vibrio fluvialis. ISRs were PCR amplified, cloned into a plasmid vector and then sequenced. As results of ISR nucleotide sequence analysis, total of 6 clones were isolated depending on the size. The clones were different in both the number and the composition of the tRNA genes, and were designated ISR-A, ISR-E, ISR-El, ISR-lA, ISR-EKV, ISR-EKAV. ISR-A contains $tRNA^{Ala}$; ISR-lA, $tRNA^{Ile}$-$tRNA^{Ala}$; ISR-EKV, $tRNA^{GIu}$-$tRNA^{Lys}$-$tRNA^{Val}$;ISE-EKAV, $tRNA^{GIu}$-$tRNA^{Lys}$-$tRNA^{Ala}$-$tRNA^{Val}$; ISR -E and E1, $tRNA^{GIu}$ clusters. ISR-EKV was shown to be a minor type out of the six ISR types and showed a very limited homology between ISR-EKV from V, fluvialis and ISRa from other Vibrio species. Therefore ISR-EKV sequence was used to design species-specific primers to detect V, fiuvialis from other Vibrio species by PCR reaction. The specificity of the primers was examined using genomic DNA of other Vibrios as templates for PCR reaction. The result showed that PCR can be a useful method to detect V. fluvialis among Vibrio species in a single PCR reaction.

A Study of the Prognostic Factors in Resected Stage I Non-Small Cell Lung Cancer (제1병기 비소세포폐암 절제례의 예후인자에 대한 연구)

  • 김창수;천수봉;조성래
    • Journal of Chest Surgery
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    • v.31 no.10
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    • pp.973-981
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    • 1998
  • Background: About 30% to 40% of the patients with pathologic stage I non-small cell lung cancer (NSCLC) die within 5 years after complete resection. The identification of poor prognostic factors and the application of additional treatment are very important to improve the survival rate in resected stage I NSCLC. Materials and methods: Sixty-eight(68) patients who had been diagnosed postoperatively between Janury 1989 and December 1995 as having stage I non-small cell lung cancer according to the TNM classification were studied. The postoperative 5-year survival rate was calculated with the Kaplan-Meier method, and clinico- histopathologic factors including age, sex, operative method, type of tumor cell, T factor, grade of the differentiation in a squamous cell carcinoma, invasion of blood vessel and expression of the nm23-H1 protein were investigated and analyzed. Results: The median survival of the entire group of patients was 58$\pm$3 months, with a 5-year survival of 58.9%. In univariate analysis, invasion of blood vessel and poor differentiation of the tumor cell in a squamous cell carcinoma significantly worsened the survival. In multivariate analysis, invasion of blood vessel and grade of the differentiation of the tumor cells in a squamous cell carcinoma remained independent prognostic factors. High expression of the nm23-H1 protein was related to a high postoperative 5-year survival in comparision with low expression of the nm23-H1 pretein (73.0% vs 50.7%), but there was no statistical significance. Conclusions: These results highlight the negative prognostic value of poor differentiation of tumor cells in a squamous cell carcinoma and invasion of blood vessel in stage I non-small cell lung cancer. Also, further studies are necessary to be determined prognostic value of the T factor and expression of the nm23 protein in non-small cell lung cancer.

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