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http://dx.doi.org/10.5012/jkcs.2002.46.2.157

In vitro Selection of RNA Aptamers which Bind to Escherichia coli tRNAVal  

Jo, Bong Rae (Department of Chemistry, Chongju University)
Publication Information
Journal of the Korean Chemical Society / v.46, no.2, 2002 , pp. 157-163 More about this Journal
Abstract
To identify RNA motifs interacting with $tRNA^{Val}$, a SELEX(Systematic Evolution of Ligands by Exponential Enrichment) was applied. Random DNA library which contains a region of ran-domized 48-mer oligonucleotide flanked by conserved sequ ence primers was transcribed into RNA pool using T7 RNA polymerase and RNA aptamers were selected with $tRNA^{Val}$ -immobilized affinity column through 14 rounds of SELEX. Some of the resulting aptamers contained a consensus sequence similar to the sequence in the loop regions of three rRNAs; C43GAAC47 sequence of 5S rRNA, G1491AAGU1495, G1379UUCC1383 sequence of 16S rRNA and C1064UUAG1068, G2110UGUA2114, C2480GACGG2485, A2600CAGU2604 sequence of 23S rRNA. These results suggest that $tRNA^{Val}$ can interact with 5S rRNA, 16S rRNA and 23S rRNA with variety in ribosome.
Keywords
$tRNA^{Val}$; In vitro Selection; RNA aptamer; $tRNA^{Val}$;
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