• 한국산학기술학회논문지
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• 제21권8호
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• pp.241-247
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• 2020

본 연구는 간호대학생의 다문화 인식과 문화적 공감이 문화적 역량에 미치는 영향을 확인하여 문화적 역량을 향상시키기 위한 기초자료를 제공하기 위하여 시도된 서술적 조사연구이다. 연구대상자는 광주와 전남 지역에 소재한 2개의 간호대학에 재학 중인 1학년에서 4학년까지의 학생을 대상으로 하였다. 자료는 2018년 9월부터 12월까지 수집하였으며, 분석을 위해 총 201명의 자료를 사용하였다. 자료 분석을 위해 SPSS 25.0 program을 이용하여 기술적 통계, t-test, ANOVA, Pearson 상관계수, 다중회귀분석을 실시하였다. 본 연구의 결과는 다음과 같다. 간호대학생의 다문화 인식((r=.451, p<.01)과 문화적 공감(r=.582, p<.01)은 문화적 역량에 유의하게 높은 양의 상관관계를 갖는 것으로 나타났다. 또한 간호대학생의 문화적 역량에 영향을 미치는 요인은 문화적 공감(β=.422, p<.001), 다문화 인식(β=.222, p<.001) 순으로 긍정적인 영향을 미치는 것으로 나타났으며, 이들 요인의 설명력은 40.5%로 확인되었다. 이러한 연구 결과는 간호대학생의 문화적 역량을 증진시키기 위해서는 다문화 인식과 문화적 공감을 높이기 위한 다양한 방안들이 적극적으로 개발되어야 하며, 이러한 방안을 개발할 때 다문화 인식보다는 문화적 공감에 더 중점을 두어야 함을 시사한다.

• 한국산학기술학회논문지
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• 제20권12호
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• pp.322-332
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• 2019

본 연구의 목적은 노인장기요양보험 방문간호사의 전문직업성, 직무만족도와 회복탄력성이 재직의도에 미치는 영향을 파악하기 위한 서술적 조사연구이다. 본 연구는 노인장기요양보험 데이터베이스에 등록된 방문간호기관에서 근무하는 방문간호사 141명을 대상으로 2019년 4월 1일 부터 2019년 5월 31까지 자가 보고형 설문지를 배부하여 자료를 수집하였다. 수집된 자료는 SPSS/WIN 22.0 프로그램을 이용하여 independent t-test, one-way ANOVA, Scheffé test, Pearson's correlation coefficient, 위계적 다중회귀분석을 이용하여 자료를 분석하였다. 연구결과, 대상자의 전문직업성 수준은 5점 만점에 평균 3.51점, 직무만족도는 5점 만점에 평균 3.27점, 회복탄력성은 5점 만점에 평균 3.79점, 재직의도는 5점 만점에 4.04점으로 나타났다. 재직의도와 전문직업성(r=.272), 직무만족도(r=.201) 회복탄력성(r=.530)은 양의 상관관계가 있었다. 최종 회귀모형에서 회복탄력성(β=.455, p<.001), 직무만족도(β=.175, p=.016) 순으로 재직의도에 유의한 영향을 미치는 것으로 나타났다. 이들 변수들의 모형 설명력은 32.0%로 나타났다(F=11.968, p<.001). 본 연구 결과를 토대로, 노인장기요양보험 방문간호사의 재직의도 향상을 위해 회복탄력성 증진 전략에 가장 관심을 기울여야 하고 다음으로 직무만족도 증진 방안 수립이 필요하다.

• 치위생과학회지
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• 제12권6호
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• pp.624-633
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• 2012

본 연구는 중년층 성인의 삶의 질 결정에 있어 건강상태의 중요성을 분석함으로써 삶의 질 향상을 위한 프로그램 기획이나 보건정책수립에 필요한 기초자료를 제공하고자 하였다. 본 연구목적을 달성하기 위해 2011년 1월 14일부터 2월 22일까지 경기 인천 지역에 거주하는 만 40세 이상의 중년층 성인에게 설문조사를 시행하였으며, t-test와 One way ANOVA 분석 및 다중선형회귀분석을 시행한 결과 다음과 같은 결론을 얻었다. 1. 일반적 특성에 따라 삶의 질 수준을 분석한 결과 일반적 삶의 질 수준은 40~50세 연령층과 고졸 이상, 가정 월평균 수입이 201만원 이상, 가족 등과 동거자 및 기혼자에서 높았다(p<0.05). 또한 구강건강관련 삶의 질은 40~50세와 고졸 이상, 가정 월 평균 수입이 201만원 이상 및 기혼자에서 높게 나타났다(p<0.05). 2. 건강상태에 따른 삶의 질 수준을 분석한 결과 일반적 삶의 질 수준은 전반적으로 건강하다고 인식하고 있고, 위장질환이 없으며, 이환된 전신질환이 없고, 매일 복용하는 약물이 없으며, 월경이 있고 우울증 증상이 9점 이하인 집단에서 높게 나타났다(p<0.05). 또한 구강건강관련 삶의 질 수준은 전반적으로 건강하다고 인식하고 있고, 당뇨병이 없으며, 이환된 전신질환이 없고, 매일 복용하는 약물이 없으며, 월경이 있고 우울증 증상이 9점 이하인 집단에서 높게 나타났다(p<0.05). 3. 구강건강상태에 따른 삶의 질 수준을 분석한 결과 일반적 삶의 질은 전반적으로 구강상태가 건강하다고 인식하고 있고, 밤시간에 입마름 등 구강건조증 증상을 전혀 인식하지 않는 집단에서 높게 나타났다(p<0.05). 또한 구강건강 관련 삶의 질은 치주질환 증상이 3개 이하이고 밤시간에 입마름 등 구강건조증 증상을 전혀 인식하지 않는 집단에서 높게 나타났다(p<0.05). 4. 일반적 삶의 질, 구강건강관련 삶의 질과 관련된 요인들의 연관성 강도를 분석한 결과 일반적 삶의 질은 우울증 증상($\beta$=-0.561)과, 구강건강관련 삶의 질은 구강건조증 증상($\beta$=-0.566)과 가장 높은 연관성을 보였다(p<0.001). 이상의 연구결과를 바탕으로 우울증과 구강건조증 인식도에 따라 일반적 삶의 질 또는 구강건강관련 삶의 질 수준이 다르게 나타남을 알 수 있었다. 따라서, 중년층에서 우울증과 구강건조증의 예방 및 조기관리는 삶의 질을 향상시키는데 크게 기여할 것으로 생각한다.

• Journal of Gastric Cancer
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• 제20권2호
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• pp.190-201
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• 2020

Purpose: This study sought to investigate the prognostic significance of tumor-infiltrating lymphocytes (TILs) in relation to tumor location within the stomach. Materials and Methods: The densities and prognostic significance of TIL subsets were evaluated in 542 gastric cancer patients who underwent gastrectomy. Immunohistochemical staining for CD3, CD4, CD8, forkhead/winged helix transcription factor (Foxp3), and granzyme B was performed. Results: Cardia cancer was associated with significantly lower densities of CD8 T-cells and higher densities of Foxp3 and granzyme B T-cells than non-cardia tumors. Multivariate analysis showed that advanced age (hazard ratio [HR], 1.023; 95% confidence interval [CI], 1.006-1.040), advanced T classification (HR, 2.029; 95% CI, 1.106-3.721), lymph node metastasis (HR, 3.319; 95% CI, 1.947-5.658), low CD3 expression (HR, 0.997; 95% CI, 0.994-0.999), and a high Foxp3/CD4 ratio (HR, 1.007; 95% CI, 1.001-1.012) were independent predictors of poor overall survival in cardia cancer patients. In non-cardia cancer patients, total gastrectomy (HR, 2.147; 95% CI, 1.507-3.059), advanced T classification (HR, 2.158; 95% CI, 1.425-3.266), lymph node metastasis (HR, 1.854; 95% CI, 1.250-2.750), and a low Foxp3/CD4 ratio (HR, 0.978; 95% CI, 0.959-0.997) were poor prognostic factors for survival. Conclusions: The densities and prognostic effects of TILs differed in relation to the location of tumors within the stomach. The contrasting prognostic effects of Foxp3/CD4 ratio in cardia and non-cardia gastric cancer patients suggests that clinicians ought to consider tumor location when determining treatment strategies.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.201-208
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• 2013

Objective: To investigate changes in the invasive capacity of gastric cancer cells in vitro after expression inhibition of T lymphoma invasion and metastasis inducing factor 1 (Tiam 1) and underlying mechanisms. Methods: Using adhesion selection, two subpopulations with high ($M_H$) or low ($M_L$) invasive capacity were separated from the human gastric cancer cell line MKN-45 ($M_0$). Tiam 1 antisense oligodeoxynucleotide (ASODN) was transfected into $M_H$ cells with liposomes, and expression of Tiam 1 mRNA and protein was determined by RT-PCR and quantitative cellular-ELISA. Changes in the cytoskeleton, invasive capacity in vitro and expression of ras-related $C_3$ botulinum toxin substrate 1 (Rac 1), integrin ${\beta}1$ and matrix metalloproteinase 2 (MMP 2) between Tiam 1 ASODN transfected $M_H$ cells and non-transfected cells were observed by HE staining, cytoskeletal protein staining, scanning electron microscopy, Boyden chamber tests and cyto-immunohistochemistry. Results: A positive correlation existed between the expression level of Tiam l mRNA or protein and the invasion capacity of gastric cancer cells. After ASODN treatment ($0.43{\mu}M$ for 48 h), Tiam 1 mRNA transcription and protein expression in $M_H$ cells were decreased by 80% and 24% respectively (P < 0.05), compared with untreated controls, while invasive capacity in vitro was suppressed by 60% (P < 0.05). Morphologic and ultrastructural observation also showed that ASODN-treated $M_H$ cells exhibited smooth surfaces with obviously reduced filopodia and microspikes, which resembled $M_0$ and $M_L$ cells. Additionally, cytoskeletal distribution dramatically altered from disorder to regularity with reduced long filament-like structure, projections, pseudopodia on cell surface, and with decreased acitn-bodies in cytoplasm. After Tiam 1 ASODN treatment, the expression of Rac 1 and Integrin ${\beta}1$ in $M_H$ cells was not affected (P > 0.05), but that of MMP 2 in $M_H$ cells was significantly inhibited compared with untreated cells (P < 0.05). Conclusion: Over-expression of Tiam-1 contributes to the invasive phenotype of gastric cancer cells. Inhibition of Tiam 1 expression could impair the invasive capacity of gastric cancer cells through modulating reconstruction of the cytoskeleton and regulating expression of MMP 2.

• Reproductive and Developmental Biology
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• 제35권3호
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• pp.197-201
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• 2011

This study was conducted to investigate the effects of donor cell treatments on the production of transgenic cloned piglets. Ear fibroblast cell obtained from NIH MHC Inbred minipig was used as control. The GalT knock-out/CD45 knock-in (GalT/CD46) transgenic cell lines were established and used as donor cells. The reconstructed GalT/CD46 embryos were surgically transferred into oviduct of naturally cycling surrogate sows (Landrace ${\times}$ Yorkshire) on the second day of standing estrus. Unlike control (1.2 kV/cm, 75.4%), the fusion rate of the GalT/CDl6 donor cells was significantly higher in 1.5 kV/cm, (84.5%) than that of 1.25 kV/cm, (20.2%) (p<0.01). When the number of the transferred embryos were more than 129, the pregnancy and delivery rates were increased to 13/20 (65%) and 5/20 (25%) compared to less then 100 group [1/6 (16.7%) and 0/6 (0%)], respectively. To analyze the effect of donor cell culture condition on pregnancy and delivery rates, the GalT/CD46 donor cells were cultured with DMEM or serum reduced medium. In serum reduced medium group, the pregnancy and delivery rates were improved to 8/12 (66.7%) and 5/12 (41.7%) compared to DMEM group [3/7 (42.9%) and 0/7 (0%)], respectively. In conclusion, it can be postulated that an appropriate fusion condition and culture system is essential factors to increase the efficiency of the production of transgenic cloned piglets.

• The Journal of Advanced Prosthodontics
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• 제11권4호
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• pp.193-201
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• 2019

PURPOSE. The aim of the present study was to evaluate the color changes of an autopolymerizing PMMA resin used for interim fixed restorations, reinforced with $SiO_2$ nanoparticles. MATERIALS AND METHODS. Silica nanoparticles were blended with the PMMA resin powder through high-energy ball milling. Four shades of PMMA resin were used (A3, B3, C3, D3) and total color differences were calculated through the equations ${\Delta}E_{ab}=[({\Delta}L*)^2+({\Delta}a*)^2+({\Delta}b*)^2]^{1/2}$ and ${\Delta}E_{00}=[(\frac{{\Delta}L^{\prime}}{K_LS_L})^2+(\frac{{\Delta}C^{\prime}}{K_CS_C})^2+(\frac{{\Delta}H^{\prime}}{K_HS_H})^2+R_T(\frac{{\Delta}C^{\prime}}{K_CS_C})(\frac{{\Delta}H^{\prime}}{K_HS_H})]^{1/2}$. Statistically significant differences between ${\Delta}E_{ab}$ and the clinically acceptable values of 3.3 and 2.7 and those between ${\Delta}E_{00}$ and the clinically acceptable value of 1.8 were evaluated with one sample t-test (P<.05). Differences among the different shades were assessed through One-Way ANOVA and Bonferroni multiple comparison tests. RESULTS. Significantly lower values were detected for all groups concerning ${\Delta}E_{ab}$ compared to the intraorally clinical acceptable values of 3.3 and 2.7. Significantly lower mean values were detected for groups B3, C3, and D3, concerning ${\Delta}E_{00}$ compared to the intraorally clinical acceptant value of 1.8. Color pigments in red-brown (A3) and red-grey (D3) shades affect the total color change to a greater extent after the reinforcement with $SiO_2$ nanoparticles compared to the red-yellow (B3) shade. CONCLUSION. Within the limitations of this in vitro study, it can be suggested that reinforcing PMMA with $SiO_2$ nanoparticles at 0.25 wt% slightly affects the optical properties of the PMMA resin without being clinically perceivable.

• 대한지구과학교육학회지
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• 제7권1호
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• pp.110-118
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• 2014

The purpose of this study was to compare the career awareness and social contribution consciousness between the elementary science gifted students and the general students. For this, survey was taken by 201 gifted students and 216 general students. Methods used are Cronbach alpha, frequency, percentage, independent t-test and Pearson's correlation coefficient. The results are as follows : 1. The gifted students have higher career awareness than the general students. The gifted students recognize their own interests, aptitudes and talents which they are concerned. 2. The gifted students have higher social contribution consciousness than the another. This shows that the gifted students have desire to be respected and positive view of social contribution. 3. There is a positive correlation between career awareness and the social contribution consciousness. According to the past studies, students' household economic status affect their view or grade which they get.. Based on these results, we suggest that a development of educational program for career awareness and social contribution consciousness which can improve gifted students' social responsibility consciousness is necessary.

• Genomics & Informatics
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• 제20권4호
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• pp.41.1-41.9
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• 2022

The pathogen Gallibacterium anatis has caused heavy economic losses for commercial poultry farms around the world. However, despite its importance, the functions of its hypothetical proteins (HPs) have been poorly characterized. The present study analyzed the functions and structures of HPs obtained from Gallibacterium anatis (NCTC11413) using various bioinformatics tools. Initially, all the functions of HPs were predicted using the VICMpred tool, and the physicochemical properties of the identified virulence proteins were then analyzed using Expasy's ProtParam server. A virulence protein (WP_013745346.1) that can act as a potential drug target was further analyzed for its secondary structure, followed by homology modeling and three-dimensional (3D) structure determination using the Swiss-Model and Phyre2 servers. The quality assessment and validation of the 3D model were conducted using ERRAT, Verify3D, and PROCHECK programs. The functional and phylogenetic analysis was conducted using ProFunc, STRING, KEGG servers, and MEGA software. The bioinformatics analysis revealed 201 HPs related to cellular processes (n = 119), metabolism (n = 61), virulence (n = 11), and information/storage molecules (n = 10). Among the virulence proteins, three were detected as drug targets and six as vaccine targets. The characterized virulence protein WP_013745346.1 is proven to be stable, a drug target, and an enzyme related to the citrate cycle in the present pathogen. This enzyme was also found to facilitate other metabolic pathways, the biosynthesis of secondary metabolites, and the biosynthesis of amino acids.

• 동서간호학연구지
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• 제26권1호
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• pp.71-79
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• 2020

Purpose: The purpose of this study was to examine the effect of head nurses' authentic leadership to nurses' organizational politics perception and organizational silence. Methods: A descriptive research design was used with a convenience sample of 201 nurses. Data were collected from Aug 7 to Sep 13, 2018 using self-reported questionnaires. Data were analyzed using descriptive statistics, t-test, ANOVA, Pearson's correlation coefficient, and multiple regression analysis. Results: The mean scores of authentic leadership of the head nurses perceived by nurses and nurses' organizational politics perception and organizational silence were 3.44 out of 5, 2.57 out of 5 and 2.62 out of 5, respectively. Nurses' organizational silence was negatively correlated with authentic leadership of the head nurse and positively correlated with nurses' organizational politics perception. Factors influencing on nurses' organizational silence were organizational politics perception, clinical career, authentic leadership of the head nurses and work life satisfaction which explained 38% of the variance. Conclusion: Based on the finding of this study, it is necessary to develop education and intervention programs in order to improve unreasonable and unfair job environment, enhance head nurses' authentic leadership, manage nurses' careers appropriately and raise the level of their work life satisfaction.