• Journal of Korean Ophthalmic Optics Society
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• v.5 no.1
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• pp.83-87
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• 2000

To investigate exhausted-medium-induced apoptosis in human corneal epithelial(HCE) cells, this study was performed DNA gel electrophoresis, M30 CytoDEATH staining and FAS-FAS ligand ELISA. SV-40 transfected cells were grown to confluency in culture for 7days. The supernatant was harvested and filtered with $0.22{\mu}m$ filter paper. Fresh HCE cells were exposed to the filtered exhausted medium for 1~2 days. Apoptotic cells were prepared for DNA extraction and run the agarose gel for DNA ladder pattern. M30 CytoDEATH was used a tool for easy and reliable determination of very early apoptosis in HCE cells. The control and exhausted medium were assayed for soluble FAS/FAS ligand protein by ELISA. HCE cells exposed to exhausted medium showed a typical DNA ladder pattern. Sporadic M30 CytoDEATH positive cells were detected among HCE cells exposed to exhausted medium. Soluble FAS/FAS ligand levels were not elevated in the exhausted medium compared to the fresh medium control. This study suggests that possible mechanism of exhausted medium induced apoptosis does not include the FAS-FAS ligand system.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.2
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• pp.293-298
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• 2009

In this contribution, a 193-bit elliptic curve cryptography coprocessor was implemented on an FPGA board. Optimized algorithms and numerical expressions which had been verified through C program simulation, should be analyzed again with HDL (hardware description language) such as Verilog, so that the verified ones could be modified to be applied directly to hardware implementation. The reason is that the characteristics of C programming language design is intrinsically different from the hardware design structure. The hardware IP which was double-checked in view of hardware structure together with algoritunic verification, was implemented on the Altera CycloneII FPGA device equipped with ARM9 microprocessor core, to a real chip prototype, using Altera embedded system development tool kit. The implemented finite field calculation IPs can be used as library modules as Elliptic Curve Cryptography finite field operations which has more than 193 bit key length.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.24 no.3
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• pp.299-309
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• 2013

A comprehensive SAR(Synthetic Aperture Radar) simulation is considered to be a complicated task since a full knowledge of the signal propagation characteristics, antenna pattern, system internal errors and interference noises should be taken into account. In high resolution target application modes, the time varying nature of target RCS(Radar Cross Section) strongly affects the generated SAR images. In this paper, in-depth SAR simulations are performed and analyzed incorporating the STK tools and MATLAB software. STK provides realistic orbit parameters while its radar module helps to extract accurate radiometric parameters of ground targets. SAR raw data corresponding to a given target is generated and processed using MATLAB simulator. The performance is measured by PSLR(Peak Sidelobe Ratio) and ISLR(Integrated Sidelobe Ratio) for a point target, which can be used as reference parameters for accurate radiometric calibration. Finally, high resolution target simulations are performed by adopting time varying target RCS characteristics.

• The Journal of the Acoustical Society of Korea
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• v.23 no.8
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• pp.583-589
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• 2004

Proposed is a speaker normalization method based on vector quantizer for continuous speech recognition (CSR) system in which no acoustic information is made use of. The proposed method, which is an improvement of the previously reported speaker normalization scheme for a simple digit recognizer, builds up a canonical codebook by iteratively training the codebook while the size of codebook is increased after each iteration from a relatively small initial size. Once the codebook established, the warp factors of speakers are estimated by comparing exhaustively the warped versions of each speaker's utterance with the codebook. Two sets of phones are used to estimate the warp factors: one, a set of vowels only. and the other, a set composed of all the Phonemes. A Piecewise linear warping function which corresponds to the estimated warp factor is adopted to warp the power spectrum of the utterance. Then the warped feature vectors are extracted to be used to train and to test the speech recognizer. The effectiveness of the proposed method is investigated by a set of recognition experiments using the TIMIT corpus and HTK speech recognition tool kit. The experimental results showed comparable recognition rate improvement with the formant based warping method.

• Journal of Astronomy and Space Sciences
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• v.32 no.3
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• pp.229-235
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• 2015

We estimated the orbit of the Communication, Ocean and Meteorological Satellite (COMS), a Geostationary Earth Orbit (GEO) satellite, through data from actual optical observations using telescopes at the Sobaeksan Optical Astronomy Observatory (SOAO) of the Korea Astronomy and Space Science Institute (KASI), Optical Wide field Patrol (OWL) at KASI, and the Chungbuk National University Observatory (CNUO) from August 1, 2014, to January 13, 2015. The astrometric data of the satellite were extracted from the World Coordinate System (WCS) in the obtained images, and geometrically distorted errors were corrected. To handle the optically observed data, corrections were made for the observation time, light-travel time delay, shutter speed delay, and aberration. For final product, the sequential filter within the Orbit Determination Tool Kit (ODTK) was used for orbit estimation based on the results of optical observation. In addition, a comparative analysis was conducted between the precise orbit from the ephemeris of the COMS maintained by the satellite operator and the results of orbit estimation using optical observation. The orbits estimated in simulation agree with those estimated with actual optical observation data. The error in the results using optical observation data decreased with increasing number of observatories. Our results are useful for optimizing observation data for orbit estimation.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39C no.11
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• pp.1011-1022
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• 2014

Currently, the wireless network environment of air node is constructed mainly of ground relay station. However, as the Korean Peninsula is composed of 70% mountainous region, there are multiple shadow zones. This is calling for effective measures to prevent aircraft from losing communication link during low-mid altitude missions. In this article we propose the utilization of Bezier Curve for estimation of aircraft flight path and control method for entering shadow zone. This method successfully estimated aircraft track, and analyzed the existence, disseminated the warning, and took measures to avoid the shadow zone before entering. This article, suggested after simulated experiments, proves that the method enables seamless communication during air operations.

• Journal of the Korea Society of Computer and Information
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• v.18 no.12
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• pp.55-64
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• 2013

In this paper, the HILS (Hardware In-the-Loop Simulation) system to analyze and to verify the performance of the targeting pod is addressed. The main functions of the targeting pod is acquiring and tracking targets to guide a LGB (Laser Guided Bomb) to the targets. For the analysis of targeting pod, the real time simulate images generation of IR and daylight cameras, sever control technology, and the analysis of laser transfer characteristics are necessary. For the real time image generation and the laser transfer characteristics analysis, off-the-shelf SDK(Software Development Kit) OKTAL-SE is used. For the servo controller, well-proven mechanism in the previous program is applied to increase servo control accuracy. To analyze the performance of a targeting pod in a realistic environment, 1553B, ARINK818 interface and etc. which are actually implemented in real combat aircrafts are applied in the system. By using the developed HILS system, the performance of currently operating targeting pods in real combat aircrafts can be analyzed and predicted. Additionally, the relationship between overall system performance and each module performance can be analyzed, the currently developed HILS system is expected to be a very useful tool to generate system development requirements of targeting pods and to reduce any possible future development risks.

• Journal of Astronomy and Space Sciences
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• v.21 no.4
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• pp.351-360
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• 2004

An orbit propagation software for the Mars orbiting spacecraft has been developed and verified in preparations for the future Korean Mars missions. Dynamic model for Mars orbiting spacecraft has been studied, and Mars centered coordinate systems are utilized to express spacecraft state vectors. Coordinate corrections to the Mars centered coordinate system have been made to adjust the effects caused by Mars precession and nutation. After spacecraft enters Sphere of Influence (SOI) of the Mars, the spacecraft experiences various perturbation effects as it approaches to Mars. Every possible perturbation effect is considered during integrations of spacecraft state vectors. The Mars50c gravity field model and the Mars-GRAM 2001 model are used to compute perturbation effects due to Mars gravity field and Mars atmospheric drag, respectively. To compute exact locations of other planets, JPL's DE405 ephemerides are used. Phobos and Deimos's ephemeris are computed using analytical method because their informations are not released with DE405. Mars Global Surveyor's mapping orbital data are used to verify the developed propagator performances. After one Martian day propagation (12 orbital periods), the results show about maximum ${\pm}5$ meter errors, in every position state components(radial, cross-track and along-track), when compared to these from the Astrogator propagation in the Satellite Tool Kit. This result shows high reliability of the developed software which can be used to design near Mars missions for Korea, in future.

• Clinical and Experimental Pediatrics
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• v.52 no.12
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• pp.1358-1363
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• 2009

Purpose:The aim of this study was to determine the prevalence of asymptomatic nasopharyngeal carriages in children using a multiplex reverse transcriptase-polymerase chain reaction (mRT-PCR) assay kit. Methods:We obtained nasopharyngeal swabs from 33 children without any underlying disease from July 25 to July 28, 2008. The children were free from the signs of respiratory tract infections at the time of sampling. DNA was extracted from the swabs and subjected to multiplex RT-PCR using a primer set for the detection of pneumococci ($Seeplex^{(R)}$ PneumoBacter ACE Detection Seegene, Seoul, Korea). The amplified PCR products were separated on 2% agarose gels and stained with either ethidium bromide or screen tape system (Lab901 Scotland, UK). Results:A total of 33 children (male, 15 female, 18) aged between 3.2 and 16.3 (median, 8.2) years were included in this study. The mRT-PCR detected colonized bacteria (Streptococcus pneumoniae, Hemophilus influenzae, Chlamydia pneumoniae, and Bordetella pertussis) in 30 children (90.9%). Of these, 13 children (39.4%) showed more than 2 bacteria: 12 children were positive for 2 bacteria (S. pneumoniae and H. influenzae) and 1 child was positive for 3 bacteria (S. pneumoniae, H. influenzae, and C. pneumoniae). Conclusion:mRT-PCR was found to be a sensitive tool for the detection of asymptomatic nasopharyngeal carriages. Clinical significances of the bacteria detected by mRT-PCR will have to be evaluated in the future.

• Biomedical Science Letters
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• v.25 no.4
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• pp.426-430
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• 2019

Currently, molecular diagnostic assays based on nucleic acid amplification tests have been shown to effectively detect mycobacterial infections in various types of specimen, however, variable sensitivity was shown in FFPE samples according to the kind of commercial kit used. The present study therefore used automated PCR-reverse blot hybridization assay (REBA) system, REBA Myco-ID HybREAD 480^®, for the rapid identification of Mycobacterium species in various types of human tissue and compared the conventional one-tube nested-PCR assay for detecting Mycobacterium tuberculosis (MTB). In conventional nested-PCR tests, 25 samples (48%) were MTB positive and 27 samples (52%) were negative. In contrast, when conducted PCR-REBA assay, 11 samples (21%) were MTB positive, 20 samples (39%) were NTM positive, 8 samples (15%) were MTB-NTM double positive, and 13 samples (25%) were negative. To determine the accuracy and reliability of the two molecular diagnostic tests, the one-tube nested-PCR and PCR-REBA assays, were compared with histopathological diagnosis in discordant samples. When conducted nested-PCR assay, 10 samples (59%) were MTB positive and seven samples (41%) were negative. In contrast, when conducted PCR-REBA test, three samples (17%) were MTB positive, 10 samples (59%) were NTM positive and four samples (24%) were negative. In conclusion, the automated PCR-REBA system proved useful to identify Mycobacterium species more rapidly and with higher sensitivity and specificity than the conventional molecular assay, one-tube nested-PCR; it might therefore be the most suitable tool for identifying Mycobacterium species in various types of human tissue for precise and accurate diagnosis of mycobacterial infection.