• Food Science of Animal Resources
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• v.24 no.4
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• pp.411-415
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• 2004

The transparisation technology for milk and milk products could be applied widely and very importantly to various determination because transparisation can economize the cost and increase with precision in the milk payment system. Component of butanone or Triton in transparisation solvent would inhibit the growth of bacteria and method. Enzymatic determination of leucocytes were proposed to evaluate milk quality as mastitis in the milk payment system, this can be easily applied to simplify automation of the determation with the lowest investment cost in milk pay system. The significance of this technique, it can be used in the quality control of raw milk and milk products, milk payment system, and programming of national dairy project. Transparisation technology is used in somatic cell counting by enzymic methods. The range of deviation for this method is 16％ in 74 samples. But the deviation is increased to 20％ when the Infoss method is used. It is affected by the percentage of epithelial cells and white blood cells in somatic cells from different animals and the stages of aging. NAgase activity has an obvious correlation with white-blood cells in milk. In the case of mastitis the white-blood cells is 90-95％ in somatic cells in milk, it is showing greater precision in measuring the state of mastitis. In conclusion the enzymic method of somatic cell counting is a relatively simple and easy method of measurement and can be easily practiced. And the importance of this method is also worth utilizing for indirect counting of Somatic cells by use of synthetic substrates to NAgase. In the future, with the further development of the research in this field, it will b possible to automatize the measurement.

• Journal of Korea Water Resources Association
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• v.51 no.12
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• pp.1181-1194
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• 2018

This study suggests a novel approach of estimating stream flow discharge using the Synthetic Aperture Radar (SAR) images taken from 2015 to 2017 by European Space Agency Sentinel-1 satellite. Fifteen small to medium sized rivers in the Han River basin were selected as study area, and the SAR satellite images and flow data from water level and flow observation system operated by the Korea Institute of Hydrological Survey were used for model construction. First, we apply the histogram matching technique to 12 SAR images that have undergone various preprocessing processes for error correction to make the brightness distribution of the images the same. Then, the flow estimation model was constructed by deriving the relationship between the area of the stream water body extracted using the threshold classification method and the in-situ flow data. As a result, we could construct a power function type flow estimation model at the fourteen study areas except for one station. The minimum, the mean, and the maximum coefficient of determination ($R^2$) of the models of at fourteen study areas were 0.30, 0.80, and 0.99, respectively.

• Journal of the Microelectronics and Packaging Society
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• v.25 no.4
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• pp.35-40
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• 2018

Simple and useful synthetic process to control the morphology of block copolymers (BCPs) is required for implementation in various device applications. However, the conventional method to use colloidal templates is not enough to realize the production of pure and massive core-shell nanoparticles due to the cost-intensive complex process. Here, we introduce a novel and facile synthesis method to realize the formation of core-shell $SiO_x$ nanoparticle power by employing an immersion annealing of a sphere-forming poly(styrene-b-dimethylsiloxane) (PS-b-PDMS) BCP. We successfully obtained a PS-encapsulated $SiO_x$ nanoparticle with a diameter of ~20 nm. In addition, we analyzed how the mixing ratio of heptane/ethanol affects the BCP morphology of self-assembled PS-b-PDMS nanoparticles, showing a worm-like structure under the optimum immersion conditions. This useful approach is expected to be extendable to other solvent-based BCP synthesis, providing a new guideline for unique BCP production.

• Journal of the Korea institute for structural maintenance and inspection
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• v.24 no.5
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• pp.61-68
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• 2020

In this study, the international standard ISO TS 16774 Part 3 Test Method for Water (wash out) Resistance and KS F 4935 ｢Sealant Injection type for water leakage maintenance of adhesive flexible rubber asphalt series｣, which are standardized as a quality control method of injection type repair materials used for water leakage cracks in underground concrete structures, are currently used in Korea. As a result, considering the performance criteria of "mass change rate -0.1%" stipulated in KS F 4935, the remaining 13 types repair materials, excluding RG-2 of synthetic rubber and UG-1 of urethane, need to be reviewed for stabilization of the loss resistance due to the flow of ground water. The results of this study are determined to be available as a basic indicator for the selection of repair materials used for cracks in concrete structures. In addition, it is expected that the results of this study can be utilized as reference data that can be reflected in the improvement of the quality of repair materials that will be researched and developed later.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.21 no.10
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• pp.1923-1928
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• 2017

This paper is a study on error correction for three-dimensional shape reconstruction based on factorization method. The existing error correction method based on factorization has a limitation of correction because it is optimized globally. Thus in this paper, we propose our new method which can find and correct the only major error influence factor toward three-dimensional reconstructed shape instead of global approach. We define the error-influenced factor in two-dimensional re-projection deviation space and directly control the error components. In addition, it is possible to improve the error correcting performance by recursively applying the above process. This approach has an advantage under noise because it controls the major error components without depending on any geometric information. The performance evaluation of the proposed algorithm is verified by simulation with synthetic and real image sequence to demonstrate noise robustness.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.3 no.2
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• pp.54-58
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• 2017

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS) is one of the powerful methods that enable analysis of small molecules as well as large molecules up to about 500,000 Da without severe fragmentation. MALDI-TOF MS, thus, has been a very useful an analytical tool for the confirmation of synthetic molecules, probing PTMs, and identifying structures of a given protein. In recent nuclear medicine, MALDI-TOF MS liner ion mode helps researcher calculate the average number of chelator(or linkage) per an antibody conjugate, such as DOTA-(or DFO-) trastuzumab for labeling a medical radioisotope. This simple technique can be utilized to improve the labeling method and control the quality at the development of antibody-based radiopharmaceuticals, which is very effected to diagnosis and therapy for in vivo tumor cells, with radioisotopes like $^{89}Zr$, $^{64}Cu$, and 177Lu. To minimize the error, MALDI-TOF MS measurement is repeatedly performed for each sample in this study, and external calibration is carried out after data collection.

• Journal of KIISE:Computer Systems and Theory
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• v.28 no.10
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• pp.491-498
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• 2001

We present a method of modeling lights from photographs taken with conventional imaging equipment. It does not use both any geometrical information and special apparatus to calculate surface reflection properties. To represent surface reflection properties of the scene, we use BRDFs which are calculated by radiance values of surface elements and light sources. And the BRDF data of each surface is further transformed to the spherical harmonic domain for efficient storage. Thus it allows to reconstruct the photo-realistic scene under different light sources, to manipulate diversely the arbitrary light source and to control exposure time of camera sensor. Moreover we test our algorithm with real image instead of synthetic image.

• Journal of the Korean Institute of Landscape Architecture
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• v.19 no.3
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• pp.55-71
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• 1991

The purpose of this thesis is to suggest objective basic data for the environmental design through the quantitative analysis of the visual quality included in the physical environment of Basemsagol valley sequence landscape. For this, visual volumes of physical elements have been evaluated by using the mesh analysis, spatial images structure of physical elements have been analyzed by factor analysis algorithm, and degree of visual quality have been measured mainly by questionnaires. Also, this study aims to understand semiotics and to grope the possibility of application to the sequence landscape assessment. A semiological approach suggests a new dimension in sequence landscape assessment, which is a contrast to the existing scientific evaluation methods. Result of this thesis can be summarized as follows. Visual volumes of the immediate vegetation, rock, bridge, road and distant vegetation are found to be the main factor determining the visual quality. Factors covering the spatial image of natural park sequence landscape have been found to be the overall synthetic evaluation, potentiality, natural quality, spatial, appeal and dignity. By using the control method for the number of factors, T.V. has been obtained as 40.22%. The characteristics of the semiological approach is qualitative, open, holistic, and experiential, whereas that of the scientific approach is quantitative, closed, reductive, and experimental.

• Journal of Microbiology and Biotechnology
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• v.8 no.1
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• pp.34-41
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• 1998

A method was developed for the controlled expression of an antimicrobial peptide magainin in Escherichia coli. A series of concatemeric magainin genes was constructed with a gene amplification vector, and fused to the 3'end of malE gene encoding the affinity ligand, E. coli maltose-binding protein (MBP). The construct directed the synthesis of the fusion protein with the magainin polypeptide fused to the C-terminus of MBP. The fusion protein was expressed in a tightly regulatable expression system which was under the control of an invertible promoter. The MBP-fused magainin monomer was expressed efficiently. However, the expression level of the MBP-fused magainin in E. coli decreased with the increasing size of multimers possibly because of the transcription and translation inhibition by the multimeric peptides. After purification using an amylose affinity column, the fusion protein was digested by factor Xa at a specific cleavage site between the monomers. The recombinant magainin had an antimicrobial activity identical to that of synthetic magainin. This experiment shows that a biologically active, antimicrobial peptide magainin can be produced by fusing to MBP, along with a promoter inversion vector system.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.02a
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• pp.533-534
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• 2012

Colloidal III-V semiconductor nanocrystal quantum dots (NQDs) have attracted attention as they can be applied in various areas such as LED, solar cell, biological imaging, and so on because they have decreased ionic lattices, lager exciton diameter, and reduced toxicity compared with II-VI compounds. However, the study and application of III-V semiconductor nanocrystals is limited by difficulties in control nucleation because the molecular bonds in III-V semiconductors are highly covalent compared to II-VI compounds. There is a need for a method that provides rapid and scalable production of highly quality nanoparticles. We present a new synthetic scheme for the preparation of InP nanocrystal quantum dots using new phosphorus precursor, P(SiMe2tbu)3. InP nanocrystals from 530nm to 600nm have been synthesized via the reaction of In(Ac)3 and new phosphorus precursor in noncoordinating solvent, ODE. This opens the way for the large-scale production of high quality Cd-free nanocrystal quantum dots.