• Title/Summary/Keyword: Synthesizing

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Purification and reaction pattern of cephalexin synthesizing enzyme from Acetobacter turbidans

  • Kang, Sang-Moo;Kim, June-Hoe;Kim, Deog-Jung;Kim, Young-Jun
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1986.12a
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    • pp.525.2-525
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    • 1986
  • Cephalexin synthesizing enzyme (${\alpha}$ amino acid ester hydrolase) was partially purified from the culture broth of Acetobacter turbidans ATCC9325 through ammonium sulfate fractionation, DEAE, CM, and Sephacryl S-200 gel filtration. The enzyme has optimum pH 6.0 and temperature, 40$^{\circ}C$ respectively. From the analysis of reaction mixtures by thin layer chromatographic and high performance liquid chromatographic techniques, it was confirmed this enzyme catalyzed simultaneously the following reactions : 1) Synthesis of cephalexin from D-${\alpha}$-phenylglycine methylester (PGM) and 7-amino 3-deacetoxy-cetoxycephalosporanic acid (7-ADCA) 2) Hydrolysis of cephalexin to form 7-ADCA and phenylglycine (PG) 3) Hydrolysis of PGM to form PG and methanol. Base on the above experimental observations, the reaction model of this enzyme was identical with that of the enzyme from Xanthomonas citri.

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Study on Dielectrics and Insulator of Diopside-Anorthite System (Diopside-Anorthite계의 유전체 및 절연체에 관한 연구)

  • Ahn, Young-Pil;Chung, Bok-Hwan;Kim, il-Ki;Lee, Kwang
    • Journal of the Korean Ceramic Society
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    • v.16 no.2
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    • pp.77-82
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    • 1979
  • Diopside-Anorthite body was easily synthesized at relatively low temperature 1225℃, compared with the synthesizing temperature 1425℃ of Anorthite. Of Diopside-Anorthite body, the synthesizing temperature was considered to be higher than 1225℃ because Gehlenite, probably formed at 1220℃, was detected by X-ray diffraction. This body has excellent physical and electrical properties, i.e. electric resistivity (1.2×1014Ωcm), low dielectric constant (6.26) and low thermal expansion coeffcient (61.9×10-7/℃). It's hardness was good enough for electrical subsidiary. In addition, this body, Diopside-Anorthite, has exellent properties for heat resisting wares.

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Activities of Sulfhydryl-Related and Phenylpropanoid-Synthesizing Enzymes during Leaf Development of Arabidopsis thaliana

  • Sa, Jae-Hoon;Park, Eun-Hee;Lim, Chang-Jin
    • BMB Reports
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    • v.31 no.6
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    • pp.554-559
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    • 1998
  • Activities of glutathione- and thioredoxin-related enzymes and phenylpropanoid-synthesizing enzymes were measured and compared in the developing leaves of Arabidopsis thaliana. Phenylalanine ammonia-lyase activity is maximal in the leaves of 2-wk-grown Arabidopsis. Tyrosine ammonia-lyase activity is maximal in the leaves of 3-wk-grown and 4-wk-grown Arabidopsis. Activity of thioitransferase, an enzyme involved in the reduction of various disulfide compounds, is higher in younger leaves than in older ones. A similar pattern was obtained in the activity of thioredoxin, a small protein known as a cofactor of ribonucleotide reductase and a regulator of photosynthesis. Activity of glutathione reductase is also higher in the younger leaves. Malate debydrogenase activity remains relatively constant during the development of Arabidopsis leaves. The results offer preliminary information for further approach to elucidate the mechanism of growth-dependent variations of these enzymes.

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A Method for Surface Reconstruction and Synthesizing Intermediate Images for Multi-viewpoint 3-D Displays

  • Fujii, Mahito;Ito, Takayuki;Miyake, Sei
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 1996.06b
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    • pp.35-40
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    • 1996
  • In this paper, a method for 3-D surface reconstruction with two real cameras is presented. The method, which combines the extraction of binocular disparity and its interpolation can be applied to the synthesis of images from virtual viewpoints. The synthesized virtual images are as natural as the real images even when we observe the images as stereoscopic images. The method opens up many applications, such as synthesizing input images for multi-viewpoint 3-D displays, enhancing the depth impression in 2-D images and so on. We also have developed a video-rate stereo machine able to obtain binocular disparity in 1/30 sec with two cameras. We show the performance of the machine.

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The Effect of H2 Flow Rate and TMS Concentration on Synthesizing Ultrafine $\beta$-SiC Powder by Vapor Phase Reaction (기상반응에 의한 $\beta$-SiC 초미분말 합성시 수소 가스유량과 TMS 농도의 영향)

  • 유용호;어경훈;소명기
    • Journal of the Korean Ceramic Society
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    • v.36 no.8
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    • pp.853-858
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    • 1999
  • To investigate the effect of H2 flow rate and TMS[Si(CH3)4] concentration on synthesizing ultrafine ${\beta}$-SiC powder by vapor phase reaction the experiment was performed at 1100$^{\circ}C$ of the reaction temperature under the condition of 200-2000 cc/min of H2 gas flow rate and 1-10% of TMS concentration respectively. The shape of ${\beta}$-SiC particles synthesized was spherical and the size of particles decreased and the distribution of particles was more uniform with increasing H2 gas flow rate. In this case Si powders were coexisted with ${\beta}$-SiC Pure and ultrafine ${\beta}$-SiC powders without Si were obtained under the condition of above 2% of TMS concentration and below 1500 cc/min of H2 gas flow rate.

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A New Method for Synthesizing Tetrazoles from Secondary Amines using Trichloroacetonitrile (이차 아민으로부터 트리클로로아세토니트릴을 사용한 테트라졸 유도체의 새로운 합성법)

  • Oh, Su-Jin
    • Journal of the Korea Institute of Military Science and Technology
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    • v.20 no.6
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    • pp.781-787
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    • 2017
  • A new method for synthesizing the tetrazole derivatives from secondary amines through cyanation/tetrazolation has been developed. Trichloroacetonitrile is used as the cyano source to synthesize N-nitrile instead of highly toxic and expensive cyanogen bromide. In this protocol, the reaction of secondary amines with various substituents proceed smoothly, and the desired tetrazole derivatives are obtained directly in fair to high yields without isolation of intermediate cyanamides.

Simultaneous growth of graphene and vertically aligned single-walled carbon nanotubes at low temperature by chemical vapor deposition

  • Hong, Suck Won;Kim, Kwang Ho;Jung, Hyun Kyung;Kim, Daesuk;Lee, Hyung Woo
    • Journal of Ceramic Processing Research
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    • v.13 no.spc1
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    • pp.154-157
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    • 2012
  • We present the simultaneous growth of single-walled carbon nanotubes and graphene with the optimal conditions of the synthesizing parameters. The dense and vertically aligned SWNTs having the length of over 100 ㎛ was grown by 2 nm-thick Fe catalytic layer. From 650 ℃, the vertically well-grown SWNTs were obtained by increasing the temperature. The severallayered graphene was synthesized with the gas mixing ratio of 15 : 1(H2 : C2H2) at 650 ℃ and higher temperatures. With these optimal conditions, the vertically well-grown SWNTs and the several-layered graphene were synthesized simultaneously. The presence of SWNTs and the layer of graphene were verified by field emission scanning electron microscopy and high resolution transmission electron microscopy. From the result of this simultaneous synthesizing approach, the possibility of one step growth process of CNTs and grapheme could be verified.

Interactive Synthesizing of Sound in Virtual Environments with considering Shape and Elevation of the Terrain (가상환경에서 지형의 형태와 고도를 고려한 인터렉티브한 사운드 합성)

  • Park, Soyeon;Park, Seong-A;Kim, Jong-Hyun
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2021.07a
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    • pp.593-596
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    • 2021
  • 본 논문에서는 지형의 고도와 기울기를 고려하여 사운드의 확산과 회절을 인터렉티브하게 표현할 수 있는 사운드 합성 기법을 제안한다. 우리의 접근 방식은 광선 추적법(Raytracing)을 기반으로 소리의 크기를 보여줄 수 있는 사운드 강도 맵을 빠르게 계산한다. 지형의 고도와 기울기 값을 고려하여 소리의 강도 맵에 가중치를 적용하여, 결과적으로 지형의 지오메트리 분석인 큰 계산 과정을 피하고 지형을 고려한 소리의 크기를 인터렉티브하게 업데이트한다. 이 과정에서 소리의 근원지에 따른 고도 간의 격차를 계산하여 소리의 크기를 자동으로 감쇠시키며, 주변 지형의 공간 기울기를 기반으로 기울기의 차에 따라 소리의 크기를 자동으로 감쇠시킨다. 본 논문에서 제안하는 방법을 이용한 소리는 실제 높낮이가 있는 현실의 지형에서 듣는 소리의 전파 및 감쇠와 유사한 형태를 보여주며, 소리의 위치에 따라서 소리 감소 패턴이 변경되고, 또한, 지형에 따라서 소리의 크기가 제어되는 결과를 보여준다.

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