• 제목/요약/키워드: Synechocystis sp. PCC6803

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Type II Isopentenyl Diphosphate Isomerase로서 Synechocystis sp. PCC6803의 sll1556의 작용 특성 (Functional Characterization of sll1556 of Synechocystis sp. PCC6803 as Type II Isopentenyl Diphosphate Isomerase)

  • 조갑연
    • 한국식품영양학회지
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    • 제23권4호
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    • pp.526-530
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    • 2010

  • Synechocystis sp. PCC6803의 type II Isopentenyl diphosphate isomerase gene(sll1556, Syidi2)의 특성을 살펴보기 위하여 ${\Delta}idi$인 E. coli $DH5{\alpha}$를 제작하고, 이 균주에서 cloning하고 발현시켰다. 라이코펜 합성 유전자들(crtE, crtB, and crtI)과 mevalonate pathway 유전자들(MvK1, MvK2, Mvd)를 함유한 ${\Delta}idi$ E. coli $DH5{\alpha}$ 균주를 mevalonate가 함유된 LB 배지에서 배양하면 mevalonate pathway 유전자들을 함유한 E. coli $DH5{\alpha}$균주는 mevalonate에 의해 생성된 isopentenyl diphosphate의 독성에 의해 매우 느린 성장을 보였다. 라이코펜 합성유전자들과 mevalonate 합성유전자들을 함유한 ${\Delta}idi$ E. coli $DH5{\alpha}$ 균주에 Syidi2를 도입한 결과, mevalonate가 함유된 LB배지에서 균체의 성장이 완전히 회복되었으며, 라이코펜이 합성되었음을 나타내는 붉은 균락이 형성되었다. 이에 따라, SyIdi1과 ECidi를 도입하여 비교한 결과, 라이코펜 합성 유전자들과 mevalonate pathway 유전자들을 함유한 ${\Delta}idi$ E. coli $DH5{\alpha}$ 균주 자체와 SyIdi1을 도입한 라이코펜 합성 유전자들과 mevalonate pathway 유전자들을 함유한 ${\Delta}idi$ E. coli $DH5{\alpha}$ 균주는 IPP의 독성에 의해 성장이 매우 느렸으나, SyIdi2, RSidi, HPidi, 및 ECidi를 함유하고 있는 ${\Delta}idi$ E. coli $DH5{\alpha}$ 균주는 균체의 성장과 라이코펜의 합성을 완전히 회복하였으며, 그중 가장 우수한 라이코펜 생합성 결과를 나타낸 것은 pSUP-LYCSyIdi2로서 균체당 라이코펜 생성량이 control 대비 2.8배이었다.

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Identification of a Glucokinase that Generates a Major Glucose Phosphorylation Activity in the Cyanobacterium Synechocystis sp. PCC 6803

  • Lee, Jung-Mi;Ryu, Jee-Youn;Kim, Hyong-Ha;Choi, Sang-Bong;de Marsac, Nicole Tandeau;Park, Youn-Il
    • Molecules and Cells
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    • 제19권2호
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    • pp.256-261
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    • 2005

  • In silico analysis of genome of the cyanobacterium Synechocystis sp. PCC 6803 identified two genes, slr0329 and sll0593, that might participate in glucose (Glc) phosphorylation (www.kazusa.or.jp/cyano). In order to determine the functions of these two genes, we generated deletion mutants, and analyzed their phenotypes and enzymatic activities. In the presence of 10 mM Glc, wild-type (WT) and slr0329 defective strain (M1) grew fast with increased respiratory activity and NADPH production, whereas the sll0593 deletion mutant (M2) failed to show any of the Glc responses. WT and M1 were not significantly different in their glucokinase activity, but M2 had 90% less activity. Therefore, we propose that Sll0593 plays a major role in the phosphorylation of glucose in Synechocystis cells.

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The Photoheterotrophic Growth of Bacteriochlorophyll Synthase-Deficient Mutant of Rhodobacter sphaeroides Is Restored by I44F Mutant Chlorophyll Synthase of Synechocystis sp. PCC 6803

  • Kim, Eui-Jin;Kim, Hyeonjun;Lee, Jeong K.
    • Journal of Microbiology and Biotechnology
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    • 제26권5호
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    • pp.959-966
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    • 2016

  • Chlorophyll synthase (ChlG) and bacteriochlorophyll synthase (BchG) have a high degree of substrate specificity. The BchG mutant of Rhodobacter sphaeroides, BG1 strain, is photosynthetically incompetent. When BG1 harboring chlG of Synechocystis sp. PCC 6803 was cultured photoheterotrophically, colonies arose at a frequency of approximately 10-8. All the suppressor mutants were determined to have the same mutational change, ChlGI44F. The mutated enzyme ChlGI44F showed BchG activity. Remarkably, BchGF28I, which has the substitution of F at the corresponding 28th residue to I, showed ChlG activity. The Km values of ChlGI44F and BchGF28I for their original substrates, chlorophyllide (Chlide) a and bacteriochlorophyllide (Bchlide) a, respectively, were not affected by the mutations, but the Km values of ChlGI44F and BchGF28I for the new substrates Bchlide a and Chlide a, respectively, were more than 10-fold larger than those for their original substrates, suggesting the lower affinities for new substrates. Taken together, I44 and F28 are important for the substrate specificities of ChlG and BchG, respectively. The BchG activity of ChlGI44F and the ChlG activity of BchGF28I further suggest that ChlG and BchG are evolutionarily related enzymes.

  • https://doi.org/10.4014/jmb.1601.01019 인용 PDF KSCI KPUBS HTML

Transcript accumulation of carotenoid biosynthesis genes in the cyanobacterium Synechocystis sp. PCC 6803 during the dark-to-light transition is mediated by photosynthetic electron transport

  • Ryu, Jee-Youn;Song, Ji-Young;Chung, Young-Ho;Park, Young-Mok;Chow, Wah-Soon;Park, Youn-Il
    • Plant Biotechnology Reports
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    • 제4권2호
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    • pp.149-155
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    • 2010

  • Expression of the genes for carotenoid bio-synthesis (crt) is dependent on light, but little is known about the underlying mechanism of light sensing and signalling in the cyanobacterium Synechocystis sp. PCC 6803 (hereafter, Synechocystis). In the present study, we investigated the light-induced increase in the transcript levels of Synechocystis crt genes, including phytoene synthase (crtB), phytoene desaturase (crtP), ${\zeta}$-carotene desaturase (crtQ), and ${\beta}$-carotene hydroxylase (crtR), during a darkto-light transition period. During the dark-to-light shift, the increase in the crt transcript levels was not affected by mutations in cyanobacterial photoreceptors, such as phytochromes (cph1, cph2 and cph3) and a cryptochrome-type photoreceptor (ccry), or respiratory electron transport components NDH and Cyd/CtaI. However, treatment with photosynthetic electron transport inhibitors significantly diminished the accumulation of crt gene transcripts. Therefore, the light induction of the Synechocystis crt gene expression is most likely mediated by photosynthetic electron transport rather than by cyanobacterial photoreceptors during the dark-to-light transition.

  • https://doi.org/10.1007/s11816-010-0130-7 인용 PDF KSCI