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Identification of a Glucokinase that Generates a Major Glucose Phosphorylation Activity in the Cyanobacterium Synechocystis sp. PCC 6803  

Lee, Jung-Mi (Department of Biology, Chungnam National University)
Ryu, Jee-Youn (Department of Biology, Chungnam National University)
Kim, Hyong-Ha (Organic Analysis Group of Korea Research Institute of Standards and Science)
Choi, Sang-Bong (Department of Biological Sciences, Myongji University)
de Marsac, Nicole Tandeau (Institut Pasteur, Unite des Cyanobacteries)
Park, Youn-Il (Department of Biology, Chungnam National University)
Publication Information
Molecules and Cells / v.19, no.2, 2005 , pp. 256-261 More about this Journal
Abstract
In silico analysis of genome of the cyanobacterium Synechocystis sp. PCC 6803 identified two genes, slr0329 and sll0593, that might participate in glucose (Glc) phosphorylation (www.kazusa.or.jp/cyano). In order to determine the functions of these two genes, we generated deletion mutants, and analyzed their phenotypes and enzymatic activities. In the presence of 10 mM Glc, wild-type (WT) and slr0329 defective strain (M1) grew fast with increased respiratory activity and NADPH production, whereas the sll0593 deletion mutant (M2) failed to show any of the Glc responses. WT and M1 were not significantly different in their glucokinase activity, but M2 had 90% less activity. Therefore, we propose that Sll0593 plays a major role in the phosphorylation of glucose in Synechocystis cells.
Keywords
Glucokinase; Glucose; Photomixotrophic Growth; Respiration; Synechocystis;
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Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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