Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Identification of a Glucokinase that Generates a Major Glucose Phosphorylation Activity in the Cyanobacterium Synechocystis sp. PCC 6803

  • Lee, Jung-Mi (Department of Biology, Chungnam National University) ;
  • Ryu, Jee-Youn (Department of Biology, Chungnam National University) ;
  • Kim, Hyong-Ha (Organic Analysis Group of Korea Research Institute of Standards and Science) ;
  • Choi, Sang-Bong (Department of Biological Sciences, Myongji University) ;
  • de Marsac, Nicole Tandeau (Institut Pasteur, Unite des Cyanobacteries) ;
  • Park, Youn-Il (Department of Biology, Chungnam National University)
  • Received : 2004.11.14
  • Accepted : 2005.01.17
  • Published : 2005.04.30
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Abstract

In silico analysis of genome of the cyanobacterium Synechocystis sp. PCC 6803 identified two genes, slr0329 and sll0593, that might participate in glucose (Glc) phosphorylation (www.kazusa.or.jp/cyano). In order to determine the functions of these two genes, we generated deletion mutants, and analyzed their phenotypes and enzymatic activities. In the presence of 10 mM Glc, wild-type (WT) and slr0329 defective strain (M1) grew fast with increased respiratory activity and NADPH production, whereas the sll0593 deletion mutant (M2) failed to show any of the Glc responses. WT and M1 were not significantly different in their glucokinase activity, but M2 had 90% less activity. Therefore, we propose that Sll0593 plays a major role in the phosphorylation of glucose in Synechocystis cells.

Keywords

Acknowledgement

Supported by : Crop Functional Genomics Center

References

  1. Cooley, J. W. and Vermaas, W. F. J. (2001) Succinate dehydrogenase and other respiratory pathways in thylakoid membranes of Synechocystis sp. Strain PCC 6803: capacity comparisons and physiological function. J. Bacteriol. 183, 4251- 4258 https://doi.org/10.1128/JB.183.14.4251-4258.2001
  2. Dahl, M. K., Schmiedel, D., and Hillen, W. (1995) Glucose and glucose-6-phosphate interaction with Xyl repressor proteins from Bacillus spp. may contribute to regulation of xylose utilization. J. Bacteriol. 177, 5467-5472
  3. Gonzali, S., Pistelli, L., De Bellis, L., and Alpi, A. (2001) Characterization of two Arabidopsis thaliana fructokinases. Plant Sci. 160, 1107-1114 https://doi.org/10.1016/S0168-9452(01)00350-8
  4. Joset, F., Buchou, T., Zhang, C. C., and Jeanjean, R. (1988) Physiological and genetic analysis of the glucose-fructose permeation system in two Synechocystis species. Arch. Microbiol. 149, 417-421 https://doi.org/10.1007/BF00425581
  5. Kaloyianni, M., Tsagias, N., Liakos, P., Zolota, Z., Christophorides, E., et al. (2004) Stimulation of $Na^+/H^+$ antiporte and pyruvate kinase activity by high glucose concentration in human erythrocytes. Mol. Cells 17, 415-421 https://doi.org/10.4313/JKEM.2004.17.4.415
  6. Mesak, L. R., Mesak, F. M., and Dahl, M. K. (2004) Bacillius subtilis GlcK activity requires cysteins within a motif that discriminates microbial glucokinases into two lineages. BMC Microbiol. 4, 6 https://doi.org/10.1186/1471-2180-4-4
  7. Meyer, D., Schneider-Fresenius, C., Horlacher, R., Peist, R., and Boos, W. (1997) Molecular characterization of glucokinase from Escherichia coli K-12. J. Bacteriol. 179, 1298-1306
  8. Mi, H., Endo, T., Ogawa, T., and Asada, K. (1995) Thylakoid membrane-bound, NADPH-specific pyridine nucleotide dehydrogenase complex mediates cyclic electron transport in the cyanobacteriun Synechocystis sp. PCC 6803. Plant Cell Physiol. 34, 661-668
  9. Mi, H., Klughammer, C., and Schreiber, U. (2000) Light-induced dynamic changes of NADPH fluorescence in Synechocystis sp. PCC 6803 and its ndhB-defective mutant M55. Plant Cell Physiol. 41, 1129-1135 https://doi.org/10.1093/pcp/pcd038
  10. Moore, B., Zhou, Z., Rolland, F., Hell, O., Cheng, W. H., et al. (2003) Role of the Arabidopsis glucose sensor HXK1 in nutrient, light and hormonal signaling. Science 300, 332-336 https://doi.org/10.1126/science.1080585
  11. Myers, J., Graham, J. R., and Wang, R. T. (1980) Light harvesting in Anacystis nidulans studied in pigment mutants. Plant Physiol. 66, 1144-1149 https://doi.org/10.1104/pp.66.6.1144
  12. Porter, R. (1988) DNA transformation. Methods Enzymol. 167, 703-712 https://doi.org/10.1016/0076-6879(88)67081-9
  13. Rolland, F., Winderickx, J., and Thevelein, J. M. (2001) Glucose- sensing mechanisms in eukaryotic cells. Trends Biochem. Sci. 26, 310-317 https://doi.org/10.1016/S0968-0004(01)01805-9
  14. Ryu, J. Y., Suh, K. H., Chung, Y.-H., Park, Y.-M., Chow, W. S., et al. (2003a) NADPH dehydrogenase-mediated respiratory electron transport in thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803 is inactive in the light. Mol. Cells 15, 240-244
  15. Ryu, J. Y., Suh, K. H., Chung, Y.-H., Park, Y.-M., Chow, W. S., et al. (2003b) Cytochrome c oxidase of the cyanobacterium Synechocystis sp. PCC 6803 protects photosynthesis from salt stress. Mol. Cells 16, 74-77
  16. Ryu, J. Y., Song, J. Y., Lee, J. M., Jeong, S. W., Chow, W. S., et al. (2004) Glucose-induced expression of carotenoid biosynthesis genes in the dark is mediated by cytosolic pH in the cyanobacteruim Synechocystis sp. PCC 6803. J. Biol. Chem. 279, 25320-25325 https://doi.org/10.1074/jbc.M402541200
  17. Schmetterer, G. R. (1994) Cyanobacterial respiration; in The Molecular Biology of Cyanobcteria, Bryant, D. A. (ed.), pp. 409-435, Kluwer, Dordrecht
  18. Wilson, J. E. (2003) Isoenzymes of mammalian hexokinase: structure, subcellular localization and metabolic function. J. Exp. Biol. 206, 2049-2057 https://doi.org/10.1242/jeb.00241