• 대한수의사회지
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• 제9권2호
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• pp.23-34
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• 1965

In 1957, a Toxoplasma infection among swine was first discovered in Korea. Thereafter, Complement Fixation Inhibition Test was applied to 2,017 swine serum samples which were obtained from all over the country except Cheju Island. Significant results are

• 대한수의학회지
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• 제12권1호
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• pp.51-58
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• 1972

Hemagglutinating antigen of Toxoplasma gondii was prepared and purified by the method of a slight modification of Tsunematsu, and the preparation of the skin test antigen (toxoplasmin) was made by means of acetone-ether treatment described by Nobute et al. With these antigens the passive hemagglutionation and skin tests were performed for the diagnosis of swine toxoplasmosis by using artificially infected pigs. The results obtained were summarized as follows: 1. The hemagglutinating antibody and the skin test antibody were demonstrated one and three weeks after infection, respectively. And these antibodies were maintained over nine weeks after infection. 2. The antigenicity of hemagglutinating antigen was stable when it was kept in frozen state, while was unstable in a liquid state. 3. Freeze-dried skin test antigen (toxoplasmin) was stable for two months or more if it was kept at $5^{\circ}C$ and room temperature, but in the liquid or reconstituted state it was unstable. 4. Freeze-dried skin test antigen could be preserved without loss of antigenicity for more than two months. 5. Passive hemagglutination test could be applied effectively at the early phase of the disease process and skin test at later phase, mainly for epidemiological survey. However, by combiniation of these methods, the more accurate results could be obtained.

• 대한수의학회지
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• 제29권4호
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• pp.567-575
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• 1989

This study was conducted to evaluate the possibility of application of a microenzyme-linked immunosorbent assay(micro-ELISA) for the serodiagnosis of specific toxoplasma antibodies in swine sera and this test was performed as a microplate system by coating the polystyrene plates with toxoplasma soluble antigen, incubated serially diluted sera, then added horse radish peroxidase labelled goat anti-swine IgG(r) conjugate followed by o-phenylenediamine as substrate. The color development by enzyme-substrate reaction was determined by the photometric reading [ELISA reader at 490nm (OD)] and visual reading. The soluble antigen was prepared from the tachyzoites in mouse peritoneal cavity. A total of 1,200 swine sera from pig slaughter-house and a total of 116 swine sera from pig breeding station (S-C farm) were tested for the detection of antibodies to Toxoplasma gondii. The results obtained were summarized as follows: 1. The optimal reactions of indirect ELISA for the test sera were determined by the dilution of antigen 1:256 and 1:3,200 of horse radish peroxidase conjugate [anti-swine IgG(r)]. 2. The specific togoplasma antibody(IgG) in pigs infected with Tp artificially were detected as the serum titers of 1:64 or 1:128 at one week postinfection. 3. Of a total of 1,200 swine sera from pig slaughter-house 505 samples of sera were detected as positive (42.1%) and of a total of 116 swine sera from S-C pig breeding station 68 samples of sera as positive (58.6%). 4. The specific antibody(IgG) detection rates against a total of 1,200 test sera from pig slaughter-house were not significant between male (43.1%) and female (40.7%). 5. The indirect ELISA was proved to be a sensitive and specific procedure for the serodiagnosis of swine toxoplasmosis and also evaluated as an effective screening test for the large scale of test samples in laboratory.

• 대한수의사회지
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• 제7권2호
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• pp.1-8
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• 1963

This concerns clinical and pathological observations on the swine toxoplasmosis which were prevalent from July to October in 1961 in Chon-Puk province. The findings obtained are summarized as follows : 1. The disease in natural occurrence appeared to be a

• 대한수의사회지
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• 제18권4호
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• pp.52-61
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• 1982

이 논문은 우리나라에서 지금까지 응용한 바있는 Toxoplasma병의 진단에 관한 시험연구의 성적을 기술하였다. 가. 우리나라에서 1957년 돈으로부터 Toxoplasma 원충을 분류한 것이 최초의 보고였다. 나. Toxoplasma병의 사후진단을 하기 위하여 동물접종시험 (1957), 조직배양시험 (1961), Acridine orange 염색법(1974) 그리고 직접형광항체법(1974)을 이용하였다. 다. Toxoplasma병의 생전진단을 하기 위하여 보체결합조지반응시험(1965), 색소시험(1965), 적혈구응집반응시험(1972) 그리고 피내반응시험(1972)을 응용하였다. 라. 야외돈에 대한 항체보유율 조사성적은 보체결합조지반응시험(1965)에서 $13.4\%$ 적혈구응집반응시험(1972)에서 $25.3\%$ 그리고 피내반응시험(1972)에서 $5.3\%$였으며 점차적 증가하는 경향을 보이고 있다. 마. Toxoplasma병에 대한 방역대책의 일단으로서 본병의 신속 정확한 진단을 하기 위하여 가축위생연구소에서는 매년 생산하는 피내반응진단액은 동물검역소에 그리고 직접형광항체진단액은 각도 가축위생시험소에 공급하고 있다.

• 대한수의사회지
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• 제16권1호
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• pp.29-34
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• 1980

The latent infection of Toxoplasma in swine, Holstein cow and Korean native cattle raised in Jeonnam prefacture during 1978 was proved serologically. Of the 127 swine, 73($57.4\%$) cases were positive for the indirect hemagglutination test, and

• 대한수의사회지
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• 제9권2호
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• pp.3-22
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• 1965

In 1908, Toxoplasma was demonstrated for the first time by Nicolle. Since then a number of isolations from birds and other mammalians including human were reported by many workers. Each strain of Toxoplasma isolated from various animals was identified as

• 한국동물위생학회지
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• 제31권1호
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• pp.87-91
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• 2008

Between March and October 2007, a total of 516 blood samples from pigs in the Gyeonggi province were examined for seroprevalence against toxoplasmosis by latex agglutination test (LAT) and the detection of antigenic particles among seropositive samples by PCR. In the LAT, 118 (22.8%) were positive, and the unadjusted percentage of seroprevalence rates of breeding and fattening pigs were significant difference. Positive rate (14.1%) in the breeding pigs was much lower than that (27.8%) of the fattening pigs (p<0.001, Pearson's Chi-square test). The antibody detection rate of sows was lower than fattening pigs, i.e., 15.8% (25/158) and 26% (93/358), respectively (P=0.011, Pearson's Chi-square test). Among 118 seropositive samples by LAT, 68 (57.6%) were positive in PCR for the detection of the toxoplasma specific-DNA. There was a statistical difference in the positive PCR reaction between the raising pigs(63/93 67.7%) and sows (5/25, 20%) (P<0.01).

• 한국동물위생학회지
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• 제15권2호
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• pp.174-183
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• 1992

This study was conducted to determine the serum antibodies against toxoplasma in swine from breeding-pig farm, pig farm and abattoir by latex agglutination(LA) test. LA test was carried out with commercial Toxo-MT kit (Eiken chemical co.). The results obtained were summerized as follows : 1. The cut-off titer of positive and negative reactions by Toxo-MT antigen used in this experiment was determined as the serum dilution of 1 ; 32. 2. positive rates of toxoplasma antibodies in 823 swine sera were 17.0%(140 cases) by LA test. 3. The toxoplasma antibody detection rates against 194 swine sera in breeding-pig farm, 273 swine sera in pig farm and 356 swine in abattoir were 46.9%(91 cases), 8.4%(23 cases) and 7.3% (26 cases) , respectively. 4. In LA test serum antibody titers in 823 test sera were shown as 51 cases (36.4%) in 1 : 32, 40(28.6%) in 1；64, 17(12.1%) in 1:128, 14(10.0%) in 1：256, 10(7.1%) in 1:512, 5(3.6%) in 1：1,024, and 3(2.1%) in 1 : 2,048. 5. Positive rates of toxoplasma antibodies in swine sera from each breeding-pig farm were 20.0∼61.9%.

• 한국동물위생학회지
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• 제27권3호
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• pp.281-288
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• 2004

As all other intensively farmed domestic species, most mortality in ostriches is closely to rearing conditions. While ostriches is also highly sensitive to stress, species-specific infectious disease play only a minor role. But investigation of ostrich's disease is not peformed almost in Korea. The study was performed to investigate the titers of antibody for Newcastle disease(ND), Infectious bronchitis(IB), Egg drop syndrome '76(EDS), Avian influenza(AI), salmonellosis, Mycoplasma gallisepticum infection(MG), Mycoplasma synoviae infection(MS), Infectious bursal disease(IBD), Brucellosis, Toxoplasmosis, Japanese encephalitis(JE), Porcine parvovirus infection, Encephalomyocarditis and Porcine reproductive respiratory syndrome (PRRS). The results obtained in the 62 ostrich sera slaughtered in Gyeongbuk province were summarized as follows: The average of antibody positive rates to ND, IB, EDS, AI(H9Nl), JE, Porcine parvovirus infection and Encephalomyocarditis by HI test were $75.8\%,\;100\%,\;0\%,\;0\%,\;51.6\%,\;50\%\;and\;56.5\%$ respectively. The antibody positive rates to salmonellosis, MG, MS by plate agglutination test were $12.9\%,\;25.8\%,\;and\;0\%$ respectively. Antibodies to disease agent such as IBD and AI by agar gel precipitation(AGP) test, Brucellosis by tube agglutination, toxoplasmosis by latex agglutination test and PRRS by IFA were all negative.