Investigation of swine toxoplasmosis by Latex agglutination and polymerase chain reaction(PCR)

Latex 응집반응 및 polymerase chain reaction을 이용한 돼지톡소플라즈마병 감염실태 조사

  • Published : 2008.03.30

Abstract

Between March and October 2007, a total of 516 blood samples from pigs in the Gyeonggi province were examined for seroprevalence against toxoplasmosis by latex agglutination test (LAT) and the detection of antigenic particles among seropositive samples by PCR. In the LAT, 118 (22.8%) were positive, and the unadjusted percentage of seroprevalence rates of breeding and fattening pigs were significant difference. Positive rate (14.1%) in the breeding pigs was much lower than that (27.8%) of the fattening pigs (p<0.001, Pearson's Chi-square test). The antibody detection rate of sows was lower than fattening pigs, i.e., 15.8% (25/158) and 26% (93/358), respectively (P=0.011, Pearson's Chi-square test). Among 118 seropositive samples by LAT, 68 (57.6%) were positive in PCR for the detection of the toxoplasma specific-DNA. There was a statistical difference in the positive PCR reaction between the raising pigs(63/93 67.7%) and sows (5/25, 20%) (P<0.01).

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References

  1. Soulsby EJL. 1962, Helminths, Arthropods and Protozoa of Domestic Animals. 7 eds. Balliere Tindal. London: 670-682
  2. Dubey JP. Leighty JC, Beal VC, et al. 1991. National seroprevalence of Toxoplasma gondii in pigs. J Parasitol 77: 517- 521 https://doi.org/10.2307/3283154
  3. Farrell RL, Docton FL, Chamberlain DM, et al. 1952. Toxoplamosis. I. Toxoplasma isolated from swine. Am J Vet Res 47: 181-185
  4. 문재봉. 1965. Toxoplasmosis에 관한 연구 1. 돈으로부터 Toxoplasma 분리. 가축위생연구소보 8: 143-160
  5. 서명득, 장동화, 주후돈. 1989. ELISA를 이용한 돼지 톡소플라즈마병의 조기 진단에 관한 연구. 대한수의학회지 29(4) : 567-575
  6. 서명득, 주후돈, 데이빗마스. 1995. Latex 응집반응을 이용한 동물의 톡소플라즈마병 진단용 kit 개발에 관한 연구. 대한수의학회지 35(3) : 583-593
  7. 서명득, 신기옥. 2001. Polymerase chain reaction 을 이용한 실험적 감염 돼지의 혈액과 조직으로부터 Toxoplasma gondii 검출. 대한수의학회지 41(1) : 89-98
  8. 김승호. 김영주. 1989. 제주도에 있어서 Toxopllasma 항체분포에 관한 연구. 1. 돼지, 고양이 및 식육취급자에 있어서의 Toxoplasma 항체분포에 대하여. 대한수의학회지 29 : 333-342
  9. 이병훈, 황보원, 변유성 등. 1992. 경남 중부지역에서의 Latex 응집반응을 이용한 돼지 톡소플라즈마병의 항체분포조사. 한가위지 15(2) : 174-183
  10. 서두석. 1979. 전남지역의 돈 Toxoplasma 감염조사 연구. 대한수의사회지 15(8) : 447-450
  11. Levine ND. 1985. Veterinary Protozology. 5 ed. Iowa. Iowa State University Press, Ames: 248-255
  12. Kijlstra A, Eissen OA, Cornelissen J, et al. 2004. Toxoplasma gondii infection in animal-friendly pig production system. Invest Ophthalmol Vis Sci 45: 3165-3169 https://doi.org/10.1167/iovs.04-0326
  13. 서명득, 주보현. 1999. 중합효소연쇄반응(PCR)을 이용한 고양이 혈액내에서의 Toxoplasma gondii 검출에 관한 연구. 대한수의학회지 39(6): 1151 -1160
  14. Stiles JE, Prade R, Green C. 1995. Detection of Toxoplasma gondii in feline and canine biological samples by use of the polymerase chain reaction. Am J Vet Res 57: 264-269