• Asian-Australasian Journal of Animal Sciences
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• 제17권11호
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• pp.1608-1614
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• 2004

This study was performed to evaluate the accuracy, validation and applicability of UV spectrophotometer (UV), Ion Chromatography (IC), and Detector tube (DT) methods for measuring ammonia (NH3) concentration in a swine confinement house and swine slurry storage tank. The mean values of $NH_{3}$ emitted from the house and slurry were 5.333 ppm and 42.192 ppm for the IC method; 4.13 ppm and 36.29 ppm for the Detector tube; and 5.417 ppm and 34.193 ppm for the UV method. The accuracy and the correlation of an ammonia level analyzed by the IC method compared to the UV method were 98% and 0.998($R^{2}$) in the swine confinement house and 94% and 0.997($R^{2}$) in the swine slurry storage tank. On the other hand, those of ammonia level measured by the Detector tube compared to the UV method were 77% and 0.957($R^{2}$) in the swine confinement house and 82% and 0.941($R^{2}$) in the swine slurry storage tank. This indicated that the accuracy and the correlation of the IC method compared to the UV method were higher than those of the Detector tube method compared to the UV method. Therefore, it was concluded that the IC method was more accurate in measuring ammonia concentration in a swine house and a swine slurry storage tank. The Detector tube method should not be applied to the swine slurry storage tank in which ammonia concentration is generally higher than 30 ppm because low accuracy is caused by a gross space between scales inscribed in the Detector tube.

• 대한수의학회지
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• 제29권4호
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• pp.567-575
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• 1989

This study was conducted to evaluate the possibility of application of a microenzyme-linked immunosorbent assay(micro-ELISA) for the serodiagnosis of specific toxoplasma antibodies in swine sera and this test was performed as a microplate system by coating the polystyrene plates with toxoplasma soluble antigen, incubated serially diluted sera, then added horse radish peroxidase labelled goat anti-swine IgG(r) conjugate followed by o-phenylenediamine as substrate. The color development by enzyme-substrate reaction was determined by the photometric reading [ELISA reader at 490nm (OD)] and visual reading. The soluble antigen was prepared from the tachyzoites in mouse peritoneal cavity. A total of 1,200 swine sera from pig slaughter-house and a total of 116 swine sera from pig breeding station (S-C farm) were tested for the detection of antibodies to Toxoplasma gondii. The results obtained were summarized as follows: 1. The optimal reactions of indirect ELISA for the test sera were determined by the dilution of antigen 1:256 and 1:3,200 of horse radish peroxidase conjugate [anti-swine IgG(r)]. 2. The specific togoplasma antibody(IgG) in pigs infected with Tp artificially were detected as the serum titers of 1:64 or 1:128 at one week postinfection. 3. Of a total of 1,200 swine sera from pig slaughter-house 505 samples of sera were detected as positive (42.1%) and of a total of 116 swine sera from S-C pig breeding station 68 samples of sera as positive (58.6%). 4. The specific antibody(IgG) detection rates against a total of 1,200 test sera from pig slaughter-house were not significant between male (43.1%) and female (40.7%). 5. The indirect ELISA was proved to be a sensitive and specific procedure for the serodiagnosis of swine toxoplasmosis and also evaluated as an effective screening test for the large scale of test samples in laboratory.

• 대한환경공학회지
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• 제34권7호
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• pp.439-444
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• 2012

실제운영 중인 40여개 양돈시설을 대상으로 사육밀도, 경영방식, 돈사환기방식, 돈분뇨 처리방식 및 악취저감제 적용특성 등의 관리현황을 파악하고 관능평가 및 악취물질 분석을 통하여 양돈시설에서 발생하는 주요 악취유발물질과 악취발생 특성을 파악하고자 하였다. 양돈시설 악취발생원 중 악취농도(복합악취)는 액비화시설(포기 상태)(평균 4,055배) > 분뇨집수조(평균 913배) > 비육사(평균 506배) > 퇴비화시설(평균 201배) 순으로 조사되었다. 비육사의 경우 슬러리 피트 내부의 돈분뇨 적체높이가 높아질수록, 사육밀도가 높을수록 발생하는 악취수준이 높은 것으로 조사되었으나, 사료첨가 유무에 따른 악취는 큰 차이를 보이지 않았다. TMA를 제외한 9종의 악취물질($NH_3$와 $H_2S$, MM, DMS, DMDS, PA, n-BA, n-VA, i-VA)이 돈사와 분뇨저장조 및 퇴비화시설에서 검출되었으며, 악취기여도가 높은 물질은 $H_2S$, MM 등의 황화계열물질과 n-BA, n-VA, i-VA 등의 휘발성 지방산류이었다. 조사 대상 양돈시설의 97.5%가 주거지역과 300 m 이내에 위치하고 있어 악취민원 발생에 취약한 것으로 조사되었다.

• 대한수의학회지
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• 제18권1호
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• pp.15-18
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• 1978

Ten strains of Salmonella were isolated from feces and lymph nodes of swine slaughtered at Daegu slaughter-house and the rate of isolation was 6.0 percent. Nine strains of Salmonella were isolated by enrichment in selenite F broth and one strain by direct culture on SS agar, but none of Salmonella were isolated from MacConkey ager and in SS broth. Among Salmonella isolated, Salmonella typhimurium occupied over half (6 strains) and the importance of Salmonella in swine for the incidence of food poisoning in man was discussed.

• 한국축산시설환경학회지
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• 제14권1호
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• pp.31-38
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• 2008

자연환기식 육성 비육돈사의 내부 사육환경을 개선하고자 돈사내부에 악취탈취장치를 설치하였다. 악취탈취장치에서 탈취한 공기는 덕트를 이용하여 돈사내부에 분배되도록 하였다. 악취탈취장치의 가동에 따른 내부의 환경은 돈사의 측벽에 설치된 윈치커튼을 닫아 관리하기 때문에 내부의 환경이 열악해지는 겨울철에 실시하였다. 악취탈취장치를 가동하면서 분석한 돈사의 내부환경 개선효과는 다음과 같다. 1. 돈사내부에 설치한 악취탈취장치에서 탈취된 후 배출된 공기를 돈사내부에 재분배함으로써 악취탈취장치 가동전보다 돈사내부의 암모니아 가스 농도를 약 33.3% 줄일 수 있는 것으로 나타났다. 2. 돈사의 암모니아 가스 저감효과는 돈사의 환기율이 $0.5m^3/m$ 두 이하인 경우에 나타났으며, 환기율이 많아지면 외부의 신선한 공기의 유입에 의해 희석되는 양이 악취탈취 장치에서 배출된 공기량보다 많아 악취탈취 장치 가동에 의한 내부 환경개선 효과는 작은 것으로 나타났다. 3. 육성 비육돈사에 악취탈취장치를 가동하면서 돼지를 입식하여 출하 때까지 사육한 결과 악취탈취장치 설치전과 비교하여 돼지의 폐사율이 약 3.8% 줄어들었으며, 탈취장치 가동후 돈사내부에 관리를 위해 들어갈 때 눈 따가움, 부유먼지 등이 크게 줄어든 것으로 나타났다.

• 한국축산식품학회지
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• 제28권5호
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• pp.623-628
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• 2008

This study was undertaken to determine the concentrations of airborne bacteria, fungi, particles, and endotoxin in swine and chicken houses. Six swine buildings and seven chicken houses were randomly selected in southern Gyonggi Province, South Korea. The geometric mean concentrations of airborne bacteria in swine and chicken houses were $2.7{\times}10^5\;CFU/m^3$ and $5.6{\times}10^7\;CFU/m^3$, respectively. The airborne bacteria concentrations in chicken houses were significantly higher than those of swine houses (p<0.05). The geometric mean concentration of airborne fungi in swine houses was $4.9{\times}10^3\;CFU/m^3$, which was higher than the value of $2.1{\times}10^3\;CFU/m^3$ found in chicken houses. The mean concentrations of airborne particles and endotoxin in swine houses were $3.48\;mg/m^3$ and $943.1\;EU/m^3$, and they were $15.43\;mg/m^3$ and $1,430.5\;EU/m^3$ in chicken houses, respectively. A significant difference between swine and chicken houses was found for total dust (p<0.05), but not for endotoxin. In this study, the concentrations of endotoxin in both swine and chicken houses as well as particles in chicken houses were high, and in about 50% of the samples exceeded the worker health safety levels of $614\;EU/m^3$ suggested in previous studies. These results may indicate a considerable respiratory hazard for workers in these environments.

• 대한수의학회지
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• 제36권1호
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• pp.131-142
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• 1996

The effect of relative humidity in swine house on swin pleuropneumonia was examined in piglets experimentally infected with Actinobacillus pleuropneumoniae serotype 5. A total of 20 piglet were grown under 30~40%, 41~50%, 51~64% and 65~80% relative humidity chambers after intratracheal inoculation of A pleuropneumoniae. Characteristic fibrinous pleuropneumonia was observed in the pigs grown at the low relative humidity groups. The detailed results were as follows : 1. Growth performance and environment conditions were lower than high relative humidity groups. 2. Characteristic histopathological findings were fibrinous pleuritis and pneumonia accompanied congestion, hemorrhage, thrombosis and edematous change. 3. Antigenic distribution of inoculated bacterium was found mainly in alveolar macrophages or accumulated foci of macrophages adjacent to necrotic area. 4. Characteristic electron microscopic findings were proliferation of type II pneumocyte with increased lamella bodies and activated alveolar macrophages with pseudopods and widening of interstitium.

• 대한수의사회지
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• 제16권1112호
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• pp.375-377
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• 1980

The analysis of the free unsaturated fatty acids and glucose in the serum of the slaughtering swine obtained from a slaughter-house showed as follows: 1. The concentration of the free unsaturated fatty acids showed significantly high Concentration. $

• Asian-Australasian Journal of Animal Sciences
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• 제33권7호
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• pp.1209-1216
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• 2020

Objective: The present study aimed to evaluate the performance of odor abatement by using two different ventilation-biofilter systems with recycled stablized swine wastewater. Methods: The performance of odor removal efficiency was evaluated using two different ventilation-biofilter-recycled wastewater arrangements. A recirculating air-flow ventilation system connected to a vertical biofilter (M1) and a plug-flow ventilation system connected to a horizontal biofilter (M2) were installed. Water dripping over the surface of the biofilter was recycled at a flow rate of 0.83 L/h in summer and 0.58 L/h in winter to reduce odorous compounds and particulate matter (PM). The experiments were performed for 64 days with M1 and M2 to investigate how these two ventilation-biofilter systems influenced the reduction of odor compounds in the model houses. Odorous compounds, NH₃ and volatile organic compounds (VOCs) were analyzed, and microclimatic variables such as temperature, humidity, and PM were monitored. Results: Ammonia concentration inside M1 was about 41% higher on average than that in M2. PM and total suspended particles (TSPs) inside M1 were about 62.2% and 69.9%, respectively, higher than those in M2. TSPs in the model house were positively correlated with the concentration of NH₃ and VOCs. Conclusion: M2 emitted lower concentration of odorous compounds than M1. Moreover, M2 could maintain the optimum temperature condition for a swine house during the cooler season. The plug-flow ventilation-horizontal biofilter system could be used for pig houses to minimize air pollution produced by swine farming activities and maintain optimum microclimate conditions for pigs.

• 한국산업보건학회지
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• 제22권4호
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• pp.309-315
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• 2012

Objectives: Animal husbandry workers could be exposed to various work hazards including toxic gases, chemicals such as pesticides or organic dust. Immunological evaluation focusing on respiratory allergic hypersensitivity occurrence was under-taken for swine farm workers as a part of the study on immunologic status of dairy barn, swine confinement, and poultry farm workers. Materials and Methods: Peripheral bloods were collected from 25 workers at the year of 2001 and 12 workers at the year of 2012 from swine farms located at Gyeonggi province, Korea. Seven adults not involved with animal husbandry were recruited at the year of 2001 from the same residential area as the swine farm workers'. Level of plasma IgE and 20 respiratory allergen-specific IgE were evaluated using commercially available ELISA kit. Results: Plasma IgE level was approximately five-fold higher in the swine farm workers regardless of the sampling year than the control subjects. Plant allergens from outdoor environments such as golden rod, pigweed, Russian thistle, or ragweed were the major allergens with positive reaction(allergen specific IgE${\geq}$0.7 IU/mL) for the swine farm workers at 2001 year. Meanwhile, house dust mite(Dermatophagoides farinae, D. pteronyssinus) and cockroach, typical indoor allergens in Korea, were the major respiratory allergens for the swine farm workers at 2012 year. Conclusions: Overall, even though our results are primitive, the results suggest that immunological function of swine farm workers could be modulated toward type-2 reactivity.