• International Journal of Industrial Entomology and Biomaterials
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• 제32권1호
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• pp.41-48
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• 2016

Mealworms, Tenebrio molitor (Coleoptera: Tenebrionidae), are widely used as food sourcefor animal rearing as well as human diet. Conventionally, mealworms raised on wheat bran. In this study, we investigated the effects of brewer's spent grain (BSG) on the growth of mealworm larvae to reduce the rearing cost. We prepared five different diets with various BSG content, 0, 10, 30, 50, and 70% of heated air dried BSG with wheat bran. We compared survivorship, larval weight, developmental duration, pupation rate, and pupal weight of five different groups of T. molitor raised on these five diets. The larval survivorships were almost similar with the control group except a group with 70% of BSG. For the larval weight, groups with 30% and 50% of BSG gained more weight than that with control treatment, 0% of BSG. For the developmental period, the groups with 30% of BSG took less time compared to the control group. In all treatment groups, more than 90% of pupation rate were observed. Especially, the group with 30% of BSG showed highest pupation rate among the five groups. Wheat bran with 30~50% of BSG was the optimal diet for successive insect rearing among the five diet treatments. Based on this study, we concluded that adding BSG to wheat bran helps to improve the quality of T. molitor and to reduce the rearing period.

• 한국응용곤충학회지
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• 제38권1호
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• pp.29-33
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• 1999

총채벌레류의 포식성 천적으로 알려진 Orius strigicollis(Poppius)의 발육과 산란에 미치는 온도의 영향을 조사하기 위한 실험조건은 처리온도 15, 20, 25, $30^{\circ}C$, 상대습도 60~80%, 광주기 16L:8D로 조절하였으며, 먹이는 목화 진딧물을 급여하였다. 처리온도별 총산란수와 성충수명은 각각 39.1, 66.4, 68.5, 47.5개와 32.6, 26.5, 13.7, 9.0일이었으며, 알기간과 부화율은 각각 18.9, 8.6, 6.1, 3.4일과 70.6, 81.8, 80.0, 91.8%이었고, 약충의 발육기간과 생존율은 각각 45.6, 21.3, 11.6, 9.4일과 26.7, 43.3, 76.7, 46.7%였다. 발육영점온도는 알 $12.4^{\circ}C$, 약충 $11.4^{\circ}C$였고, 유효적산온도는 알 64.9일도, 약충 172.4일도이다.

• 대한골관절종양학회지
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• 제3권1호
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• pp.1-8
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• 1997

Osteosarcoma is the most common primary bony malignancy and its survivorship has been progressed markedly through refined chemotherapy and surgery. But still there are many non-responders and analysis of prognostic factors may be helpful for them. Two hundred and sixty-six patients were enlisted between Mar, 1985 and Sep. 1994. Among them our inclusion criteria were: 1)primary, nonmetastatic classical osteosarcoma 2)extremity in location 3)no prior treatment at other institute and completed neoadjuvant chemotherapy and surgery according to our protocol. One hundred and eleven cases were eligible. Analyzed factors were:age, sex, location, tumor size, and pathologic response. Statistical methods were log-rank test for univariate and Cox's test for multivariate analysis. Male to female ratio was 69:42 with an average age of 17.2 years. Locations of tumor were distal femur 59, proximal tibia 29, and proximal humerus 8. Tumor size were measured by its maximal diameter and 48 cases were above 10cm and 47 cases were below 10cm. For pathologic response, 57 cases showed more than 90% and 54 cases were less than that. Limb salvage procedure was 101 cases and amputation was 10 cases and their local recurrence rate were 3.6%. Average follow-up period was 24(9-78.2) months and their final status was CDF 86, AWD 8, NED 5, and DOD 12 cases. In univariate study: type of operation(p=0.005), tumor size(p=0.005), and pathologic response(p=0.02) were significant variables. Pathologic response(p=0.03) and type of operation(p=0.01) were meaningful prognostic factors on multivariate analysis. But the latter result was interpreted as a bias, so pathologic response remained as a sole meaningful prognostic factor. More aggressive chemotherapy will be needed to improve the survival.

• Ocean and Polar Research
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• 제27권2호
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• pp.205-214
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• 2005

From ancient Antarctic glacier ice, we extracted total genomic DNA that was suitable for prokaryotic 16S rDNA gene cloning and sequencing, and bacterial artificial chromosome (BAC) library and end-sequencing. The ice samples were from the Dry Valley region. Age dating by $^{40}Ar/^{39}Ar$ analysis on the volcanic ashes deposited in situ indicated the ice samples are minimum 100,000-300,000 yr (sample DLE) and 8 million years (sample EME) old. Further assay proved the ice survived freeze-thaw cycles or other re-working processes. EME, which was from a small lobe of the basal Taylor glacier, is the oldest known ice on Earth. Microorganisms, preserved frozen in glacier ice and isolated from the rest of the world over a geological time scale, can provide valuable data or insight for the diversity, distribution, survival strategy, and evolutionary relationships to the extant relatives. From the 16S gene cloning study, we detected no PCR amplicons with Archaea-specific primers, however we found many phylotypes belonging to Bacteria divisions, such as Actinobacteria, Acidobacteria, Proteobacteria $({\alpha},\;{\beta},\;and\;{\gamma})$, Firmicutes, and Cytophaga-Flavobacterium-Bacteroid$. BAC cloning and sequencing revealed protein codings highly identical to phenylacetic acid degradation protein paaA, chromosome segregation ATPases, or cold shock protein B of present day bacteria. Throughput sequencing of the BAC clones is underway. Viable and culturable cells were recovered from the DLE sample, and characterized by their 16S rDNA sequences. Further investigation on the survivorship and functional genes from the past should help unveil the evolution of life on Earth, or elsewhere, if any.

• 한국수산과학회지
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• 제43권3호
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• pp.239-245
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• 2010

The effects of sediment and water movement on the survival and growth of Campylaephora hypnaeoides J. Agardh were examined in culture to elucidate why this species grows as an epiphytic alga. The survival and growth rates of Campylaephora tetraspores declined as the sediment concentration increased under both water-movement and no-water-movement treatments. After 5-day cultures under various sediment treatments, the respective survival and growth rates ranged from 27.32 to 78.63% and 0.22 to 0.29 $day^{-1}$ under the no-water-movement and from 15.66 to 82.69% and 0.19 to 0.31 $day^{-1}$ under the water-movement treatments. The maximum survival and growth of C. hypnaeoides tetraspores occurred at 20 and 40 ${\mu}mol$ photons $m^{-2}s^{-1}$, respectively. The survival of Campylaephora tetraspores and germlings declined with increasing dark period, but the tolerance of total darkness based on the survival rates was two-times greater for 5-day old sporelings than for tetraspores after 12 days. In conclusion, Campylaephora hypnaeoides grows as an epiphytic alga because its survivorship and growth decline rapidly with greater water movement and sedimentation and with lower light intensities.

• Animal cells and systems
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• 제14권4호
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• pp.315-322
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• 2010

We studied and compared the age structure, body size, and growth rates of field populations of two Korean salamander species (Hynobius yangi and Hynobius quelpaertensis) to elucidate important aspects of basic population dynamics of these two endemic Hynobius species. In both populations, females were sexually mature at three years of age, while H. yangi and H. quelpaertensis males matured at two and three years of age, respectively. Both males and females of H. yangi and H. quelpaertensis attained a maximum age of 11 years and 10 years, respectively. In both species, the snout-vent length (SVL) and body weight (BW) of the females were greater than those of the males. The SVL, BW, and asymptotic SVL of both male and female H. yangi were smaller than those of H. quelpaertensis. The adult growth rates after sexual maturation of male and female H. yangi were lower than those of H. quelpaertensis, possibly resulting in the smaller body size of the former, although overall growth coefficients were not significantly different between the two species. We also compared the age structure and growth rates of three Korean and three Japanese species of Hynobius.

• International Journal of Industrial Entomology and Biomaterials
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• 제21권1호
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• pp.133-137
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• 2010

The perilla leaf pyralid moth, Pyrausta panopealis Walker (Lepidoptera: Pyralidae), is a serious pest damaging to leaf perilla. In order to establish the life parameters of P. panopealis for eventual purpose of control, the developmental span of each stage was investigated under five temperature regimes ($20^{\circ}C{\sim}30^{\circ}C$). The larval period of P. panopealis was longest as 26.8 days at $20^{\circ}C$ and shortened as temperature goes up to $30^{\circ}C$ as 11.3 days. Survivorship of the larval P. panopealis was the highest at $27.5^{\circ}C$ as 82.5%, whereas that of other temperatures ranged from 40% ($20^{\circ}C$) to 60.0% ($30^{\circ}C$), indicating that the P. panopealis appears to favor somewhat higher temperature. In addition to larval period, the duration of egg, prepupal, and pupal period also were shortened sharply as temperature goes up, whereas the duration of adult stage shortened a maximum of only two days as temperature goes up. After the perilla leaf pyralid moths were successfully stabilized in indoor environment the larvicidal efficacy of ten on-the-market environmentfriendly agricultural materials (EFAMs) that were previously selected from the result of other moth species was tested. Seven of the ten tested showed more than 90% of mortality within 12 hrs and reached nearly up to 100% within 24 hrs, but the remaining three showed less than ~70%.

• 생태와환경
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• 제42권1호
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• pp.107-114
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• 2009

Daphnia pulex와 D. galeata에 대한 실잠자리류 유충의 포식 특성과 이들이 포식자로서 분비하는 화학물질에 의해 유도되는 두 종의 물벼룩의 생활사 및 형태적 방어 반응을 어류 포식자인 배스에 대해 나타내는 반응과 비교분석 하였다. 실잠자리류의 유충은 낮과 밤에 관계없이 섭식활동을 하며 상대적으로 작은 크기의 물벼룩을 주로 포식하였고, 소형의 D. galeata에 대한 포식율이 높았다. D. pulex는 실잠자리류 유충과 배스가 분비한 카이로몬에 대하여 생활사 및 형태적 변화를 나타내었다. D. galeata는 실잠자리류 유충에 상대적으로 높은 포식압에도 불구하고 배스의 카이로몬에 대한 반응만을 나타냈다. 본 실험의 결과는 다양한 포식자에 대응하는 생존전략이 물벼룩 종에 따라 다를 수 있음을 보여주었다.

• 보건행정학회지
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• 제30권4호
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• pp.433-437
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• 2020

Cancer is a disease which has the huge burden in worldwide, and cancer is the number one cause of death in Korea. At this point, the new framework for cancer monitoring index is required for regional cancer monitoring. Especially, cancer survivors are the important target which is rapidly increasing recently, also cancer survivor's quality of care should be considered in the cancer monitoring index framework. To develop the Multidimensional Cancer Monitoring Index considering cancer survivor's quality of care, we took into account cancer continuum which including prevention, detection, diagnosis, treatment, survivorship, assessment of quality of care and monitoring cancer patient, and end-of life care for stage. For target, components of health care delivery system such as patient, family, provider, payer, and policy maker are included. Also, Donabedian model which is a framework for examining health services and evaluating quality of health care such as structure, process, and outcome is applied to contents. This new cancer monitoring framework which includes multidimensional components could help to develop regional cancer monitoring index, and to make national cancer management and prevention policy in the future.

• Journal of Plant Biotechnology
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• 제47권4호
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• pp.324-329
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• 2020

Reed (Phragmites spp.) is a rhizomatous plant of the Poaceae family and is known as high tolerant plant to heavy metal contaminants. This plant is widely recognized as a Cd root-accumulator, but improved heavy metal tolerance and uptake capacity are still required for phytoremediation efficiency. To enhance capacity of hyperaccumulator plants, ethyl methane sulfonate (EMS) as chemical mutagen has been introduced and applied to remediation approaches. This study aimed to select EMS-mutagenized reeds representing high Cd resistance and large biomass and to investigate their ability of Cd accumulation. After 6 months cultivation of M₂ mutant reeds under Cd stress conditions (up to 1,500 µM), we discovered seven mutant individuals that showed good performances like survivorship, vitality, and high accumulation of Cd, particularly in their roots. Compared to wild type (WT) reeds as control, on average, dry weight of mutant type (MT) reeds was larger by 2 and 1.5 times in roots and shoots, respectively. In addition, these mutant plants accumulated 6 times more Cd, mostly in the roots. In particular, MT8 reeds showed the greatest ability to accumulate Cd. These results suggest that EMS mutagenesis could generate hyperaccumulator plants with enhanced Cd tolerance and biomass, thereby contributing to improvement of phytoremediation efficiency in Cd-contaminated soil or wastewater. Further studies should focus on identifying Cd tolerance mechanisms of such EMS-mutagenized plants, developing techniques for its biomass production, and investigating the practical potential of the EMS mutants for phytoremediation.