• BMB Reports
• /
• v.31 no.1
• /
• pp.101-105
• /
• 1998

Immunoassay of botulinum toxin (BTX) B type was investigated using two typed of biosensors: light addressable potentiometric sensor (LAPS) and surface plasmon resonance (SPR) sensor. Urease-tagged and immuno-filtration capture method have been used for LAPS. Tag-free and direct binding real-time detection method have been used for SPR sensor. The detection limit of sandwich assay format with LAPS was 10 ng/ml, which was the lowest among methods tested. SPR has the advantage of being more convenient because tag-free direct binding assay can be used and reaction time was reduced, regardless of low sensitivity. This result shows that sandwich assay format with LAPS can be used as an alternative method of BTX mouse bioassay which is known as the most sensitive method for the detection of BTX.

• KSBB Journal
• /
• v.17 no.2
• /
• pp.121-136
• /
• 2002

Immunosensors are of great interest because of their potential utility as specific, simple, label-free, direct detection means and provision of reduction in size, cost and time of analysis comparing with conventional immunoassay. In the last two decades, many reports have been published on the use of immunosensors for a wide range of applications to clinical diagnostics, pharmaceutical chemistry, environmental monitoring, biotechnology and food industries. There are also numerous transduction techniques developed such as electrochemical techniques, piezoelectric crystal, and surface plasmon resonance receiving much attention for the direct monitoring of immune reactions at solid surfaces. In this article, the principles, characteristics, structures, fonctions and clinical applications of immunosensors were reviewed

• Proceedings of the Optical Society of Korea Conference
• /
• 2008.02a
• /
• pp.125-126
• /
• 2008

We investigate characteristics of gold and silver metal strip waveguides based on long range surface plasmon polaritons (LRSPPs) along thin metal strips embedded in a polymer for practical applications at the wavelengths of 1.31 and 1.55 um. Guiding properties of the metal strip waveguides are theoretically and experimentally evaluated. In addition, surrounding technologies such as the development of TM-mode laser, coupling properties, as well as high-speed digital signal transporting experiments will be explained for board-to-board optical interconnections by using the LRSPP waveguides.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2009.10a
• /
• pp.1220-1223
• /
• 2009

The effect of silver (Ag) nano-particles on OLEDs was investigated by using a finite difference time domain (FDTD) tool. The proposed OLEDs employed Ag nanoparticles thermally deposited in a high vacuum on a cathode. The emission rate of the exciton was improved by 1.8 fold compared to the conventional OLEDs without Ag nano-particles. Less spacing between the dipole source and the Ag nano-particles resulted in a larger emission rate of the exciton in the OLED with nano-particles. The size of the Ag nano-particles was proportional to the emission rate of the exciton in a range of nano-meter scale of nano-particles. The enhancement of the emission rate of the exciton due to Ag nano-particles caused the improvement in the efficiency of the proposed OLED.

• Journal of the Optical Society of Korea
• /
• v.6 no.3
• /
• pp.83-86
• /
• 2002

Nanoscopic emission from periodic nano-hole arrays in thick metal films is studied experimentally. The experiments give direct evidence for SP excitations in such structures. We show that the symmetry of the emission is governed by polarization and its shape is defined the interference of SP waves of different diffraction orders. Near-Held pattern analysis combined with the far-Held reflection and transmission measurements suggests that the SP eigenmodes of these arrays may be understood as those of ionic plasmon molecules.

• Proceedings of the KIEE Conference
• /
• 2006.07c
• /
• pp.1615-1616
• /
• 2006

본 논문에서는 표면 플라즈몬 공명(Surface Plasmon Resonance) 센서의 여러 파라미터를 동시에 만족시키는 설계기법을 제시하였다. 설계 파라미터는 반사광 dip의 3dB bandwidth와 dip의 깊이이고 목적함수는 해석치와 목표치의 차이 벡터의 norm으로 정의하였다. 설계 변수는 박막된 각 금속 충의 두께로 하였고 SPR 센서의 스펙트럼을 해석하기 위해 광학 어드미턴스 기법을 이용하였다. 최적화 기법은 (1+1) 진화 알고리즘을 사용하였다. 설계방법을 3층 구조의 SPR 센서에 적용하여 최적 설계한 파라미터는 초기값에 비해 3dB bandwidth는 4.8nm, dip의 깊이는 1.1dB 향상되었다.

• Proceedings of the Korean Information Science Society Conference
• /
• 2004.04b
• /
• pp.250-252
• /
• 2004

나노 기술의 개발과 마이크로어레이(microarray)의 등장으로 생물학자동을 한번에 보다 라르고 신속하게, 대량의 실험을 처리할 수 있게 되었다. 이와 발 맞추어 마이크로어레이를 이용한 다양한 실험 방법들이 개발되었다. 형광 염료(fluorescence dye)를 이 용한 관찰 방법 이 널리 이용되고 있으나, 관찰되는 형광 염료의 밝기가 실험 환경(pH, 온도)에 매우 민감하게 반응하며, 단백질을 포함한 많은 분자 물질들이 형광을 내지 않기 때문에 마이크로어레이를 이용한 분석 대상 물질들의 개수가 제한을 받는다. 본 논문에서는 직접적인 형광 염료의 사용 없이, SPR(Surface Plasmon Resonance)을 이용한 마이크로어레이 분석 시스템에서 스팟(spot)의 밝기(intensity)를 측정하기 위한 효율적인 전처리 과정을 제안하고자 한다. 전처리 과정은 크게 프로젝티브 왜곡 효과 제거, 스팟의 위치 추적, 스팟의 영역 추출, 정규화 된 스팟의 밝기 측정으로 나누어진다. 특히, 이러한 과정을 거쳐서 측정된 밝기는 반응 유무의 관찰뿐만이 아니라, 실험 물질의 양적인 측정에도 이용되기 때문에 정확한 스팟의 밝기 측정에 중점을 두고자 한다.

• Journal of the Korean Physical Society
• /
• v.73 no.9
• /
• pp.1283-1288
• /
• 2018

In this work, we investigate polarization-dependent excitation of the propagating surface plasmon polariton (SPP) modes in gold nanowires (Au NWs) combined with gold nanoparticles (Au NPs). The light coupling from focused light to SPPs on Au NWs is investigated for different structural combinations of Au NWs with Au NPs, using full-wave finite-element numerical simulations. The results show that the excitation of SPPs changes remarkably on varying the orientation of the NP on NW or the polarization angle of the incident light. Metallic NWs combined with NPs can be applied to the polarization-resolved SPP coupling in various optical and optoelectronic devices including photonic circuits and optical sensors.

• Journal of Electrochemical Science and Technology
• /
• v.12 no.2
• /
• pp.167-172
• /
• 2021

he progressively improved sensing sensitivity (∆λ_SPR/∆n, nm/RIU) to detect the refractive index is observed on the SPR platform of an Au-covered epoxy gratings in an increase in potential cycling in a typical three-electrode cell. Here, a DVD-R optical disc was used as a structure template to prepare an Au-covered epoxy gratings, and the newly formed reverse track pitch structure on the epoxy substrate was used as a working electrode directly in aqueous sulfuric acid solution. It is expected that Au reconstruction by potential cycling in sulfuric acid electrolyte increases the packing density of Au atoms in the grain boundary and improves the propagation of electromagnetic waves.

• Proceedings of the Korean Magnestics Society Conference
• /
• 2007.05a
• /
• pp.261.1-261.1
• /
• 2007