• Journal of Preventive Medicine and Public Health
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• v.55 no.3
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• pp.263-272
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• 2022

Objectives: Infections with hepatitis B, C, and D virus (HBV, HCV, and HDV) are a major public health problem and lead to serious complications such as cirrhosis and hepatocellular carcinoma. We aimed to determine the seroprevalence of hepatitis B surface antigen (HBsAg), anti-HCV, anti-HDV immunoglobulin G, alpha-fetoprotein (AFP), and dual and triple hepatitis virus infections in Mongolia. Methods: A total of 2313 participants from urban and rural regions were randomly recruited for this cross-sectional study. A questionnaire was used to identify the risk factors for hepatitis virus infections, and the seromarkers were measured using immunoassay kits. Results: Among all participants, the prevalence of HBV, HCV, and HDV was 15.6%, 36.6%, and 14.3%, respectively. The infection rates were significantly higher in females and participants with a lower education level, rural residence, older age, and a history of blood transfusion. HBV and HCV co-infection was found in 120 (5.2%) participants and HBV, HCV, and HDV triple infection was detected in 67 (2.9%) participants. The prevalence of elevated AFP was 2.7%, 5.5%, and 2.6% higher in participants who were seropositive for HBsAg (p=0.01), anti-HCV (p<0.001), and anti-HDV (p=0.022), respectively. Elevated AFP was more prevalent in participants co-infected with HBV and HCV (5.8%, p=0.023), HBV and HDV (6.0%, p<0.001), and triple-infected with HBV, HCV, and HDV (7.5%) than in uninfected individuals. Conclusions: Nearly half (49.8%) of the study population aged ≥40 years were infected with HBV, HCV, or HDV, and 22.4% had dual or triple infections.

• Journal of Yeungnam Medical Science
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• v.14 no.2
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• pp.359-369
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• 1997

We studied the expression of the cell surface antigen associated with myeloid and lymphoid leukemias on bone marrow or peripheral blood blast cells from 153 leukemic patients including 61 cases of acute myelogenous leukemias(AML), 46 of acute lymphocytic leukemias(ALL) and 12 of acute leukemias. They were analyzed by direct or indirect immunofluorescence method for reactivity with the monoclonal antibodies to B cells(CD10, CD19, SmIg), T cells(CD2, CD5, CD7, CD3, CD4, CD8), myeloid antigen(CD13, CD14, CD33, CD61) and a nonspecific antigen, HLA-DR. Lymphoid associated markers detected on AML is CD7 32.8%, CD10 14.8%, CD5 13.1%, CD2 6.6% and CD19 1.6%. TdT was positive in 4.9% of AMLs. Hybrid leukemias were 8 cases out 61 AML cases and were mainly composed of monocytic lineage, M4 and M5a. Myeloid markers detected in ALL were CD13 2.2% and CD33 2.2%. In this study, immunologically classified ALLs were composed of 65.2% of CALLA (+) B precursor type, 10.9% of CALLA (-) B precursor pattern, 8.7% of T cell type, 2.2% of B cell type, 4.5% of mixed lymphoid lineage(B&T), 2.2% of undifferentiated leukemia, and 6.5% of hybrid leukemia. Twelve cases of acute leukemias ware finally diagnosed to be 5 cases of hybrid leukemia, 3 cases of B lineage, 3 case of T lineage and 1 case of mixed lymphoid(B&T) leukemia. In summary, we think the best method for typing acute leukemias is by using a combination of FAB classification and immunophenotying.

• The Korean Journal of Physiology and Pharmacology
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• v.22 no.2
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• pp.203-213
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• 2018

Tumor undergo uncontrolled, excessive proliferation leads to hypoxic microenvironment. To fulfill their demand for nutrient, and oxygen, tumor angiogenesis is required. Endothelial progenitor cells (EPCs) have been known to the main source of angiogenesis because of their potential to differentiation into endothelial cells. Therefore, understanding the mechanism of EPC-mediated angiogenesis in hypoxia is critical for development of cancer therapy. Recently, mitochondrial dynamics has emerged as a critical mechanism for cellular function and differentiation under hypoxic conditions. However, the role of mitochondrial dynamics in hypoxia-induced angiogenesis remains to be elucidated. In this study, we demonstrated that hypoxia-induced mitochondrial fission accelerates EPCs bioactivities. We first investigated the effect of hypoxia on EPC-mediated angiogenesis. Cell migration, invasion, and tube formation was significantly increased under hypoxic conditions; expression of EPC surface markers was unchanged. And mitochondrial fission was induced by hypoxia time-dependent manner. We found that hypoxia-induced mitochondrial fission was triggered by dynamin-related protein Drp1, specifically, phosphorylated DRP1 at Ser637, a suppression marker for mitochondrial fission, was impaired in hypoxia time-dependent manner. To confirm the role of DRP1 in EPC-mediated angiogenesis, we analyzed cell bioactivities using Mdivi-1, a selective DRP1 inhibitor, and DRP1 siRNA. DRP1 silencing or Mdivi-1 treatment dramatically reduced cell migration, invasion, and tube formation in EPCs, but the expression of EPC surface markers was unchanged. In conclusion, we uncovered a novel role of mitochondrial fission in hypoxia-induced angiogenesis. Therefore, we suggest that specific modulation of DRP1-mediated mitochondrial dynamics may be a potential therapeutic strategy in EPC-mediated tumor angiogenesis.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.17 no.2
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• pp.87-94
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• 2012

To compare monthly variations of phytoplankton biomass and community composition between in seawater and sediment of the Gomso Bay (tidal flat: approximately 75%), the photosynthetic pigments were analyzed by HPLC every month in 1999 and every two months in 2000. Ambient physical and chemical parameters (temperature, salinity, nutrients, dissolved oxygen, and chemical oxygen demand) were also examined to find the environmental factors controlling structure of phytoplankton community. The temporal and spatial variations of chlorophyll a concentration in seawater were correlated well with the magnitude of freshwater discharge from land. The biomass of microphytobenthos at the surface sediments was lower than that in other regions of the world and 2-3 times lower than phytoplankton biomass integrated in the seawater column. Based on the results of HPLC pigment analysis, fucoxanthin, a marker pigment of diatoms, was the most prominent pigment and highly correlated with chlorophyll a in seawater and sediment of the Gomso Bay. These results suggest that diatoms are the predominant phytoplankton in seawater and sediment of the Gomso Bay. However, the monthly variation of chlorophyll a concentration in seawater at the subtidal zone was not a good correlation with that in sediment of the Gomso Bay. Although pelagic plankton was identified in seawater by microscopic examination, benthic algal species were not found in the seawater. These results suggest that contribution from the suspended microphytobenthos in the tidal flat to the subtidal zone of the Gomso Bay may be low as a food source to the primary consumer in the upper water column of the subtidal zone. Further study needs to elucidate the vertical and horizontal transport magnitude of the suspended microphytobenthos in the tidal flat to the subtidal zone.

• Clinical and Experimental Pediatrics
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• v.51 no.1
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• pp.73-77
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• 2008

Purpose : p16 gene, mapped to the 9p21 chromosomal region, has emerged as a candidate tumor suppressor gene in human neoplasm. It is an inhibitor of cyclin-dependent kinase and inhibits Rb phosphorylation. In a variety of tumors including childhood acute lymphoblastic leukemia (ALL), deletion and/or mutation of the p16 gene has been found. Despite their high frequency, the prognostic importance of p16 alterations is still controversial in ALL and has been reported to be either unfavorable or similar to that of other patients. We studied the correlation between loss of p16 protein confirmed by immunohistochemical staining and clinical outcomes of patients diagnosed as ALL. Methods : We performed an immunohistochemical staining for p16 protein in 74 cases of bone marrow biopsy slide initially diagnosed as ALL between January 1998 and December 2006. We reviewed the clinical manifestations, laboratory findings, treatment outcomes retrospectively. Results : Of 74 slides, 12 were negative for p16 protein. Seven were males and 5 were females with a median age at diagnosis was 5.8 (1.3-18.8) years. Initial WBC were 17,225 $(500-403,300)/{\mu}L$. By immunologic surface marker analysis, 7 patients were early pre-B CALLA (+) and 5 patients were T-cell ALL. Two patients of intermediate risk group had relapsed and died. Three patients had family history of breast cancer. Four patients died and overall survival rates were $53.5{\pm}18.7%$. Conclusion : Loss of p16 protein is supposed to be an independent risk factor of childhood ALL associated with poor outcomes. In clinical setting, the clinician must take into account p16 status, not only at the genomic but also at the protein level. Further clinical experience on thoroughly investigated cases will help a better understanding between p16 status and clinical outcomes.

• The Korean Journal of Food And Nutrition
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• v.15 no.4
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• pp.321-325
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• 2002

For examination, amaranth was first dried its surface after sufficient soaking in water. Dried amaranth was roasted when the moisture contents reached 30∼50%, its nasty smell disappeared and its color turned to yellow and it was puffed. Pretreated amaranth was added to biscuit for the improvement of apparence and merchandise value. The maximum expansion was reported at the moisture percentage of 130∼160% according to the examination of expansion and moisture percentage. In order to compare gelatinization of different processing, three different amaranth were prepared as follows ; Raw amaranth, steamed/dried amaranth, and roasted amaranth. The degree of gelatinization was increased as the percentage of moisture was increased and the degree of gelatinization of roasted amaranth was higher than the one of steamed/dried amaranth, their moisture contents were 62.10% and 57.59%, respectively In addition, the hardness($\times$10$\^$5/dyn/㎠) of roasted amaranth was showed lower values than that of steamed amaranth and raw amaranth were showed the large values. After examining biscuits containing each amaranth, raw amaranth had problem with nasty smell and bad texture, and streamed/dried amaranth were able to remove viscosity but turned brown. Biscuits containing roasted amaranth had good smell and texture, and besides the color of biscuits became bright because roasted amaranth turned white. Biscuits tasted best when it contained 5% of roasted amaranth. The rancidity of biscuits with 5% roasted amaranth were proceeded slowly while roasted amaranth itself had high acid value and peroxide value. For the safety from oxidation, it was fairly safe for about 6 months.

• The Journal of Korean Society for Radiation Therapy
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• v.27 no.1
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• pp.73-78
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• 2015

Purpose : Under the assumption of change to the amplitude based sorting, the study will use four dimensional computed tomography imaging (4DCT) arrayed using the phase based sorting to analyze the respiratory phase difference. Materials and Methods : The study analyzed the 4DCT (4-dimensional computed tomography) images of 10 liver cancer patients that were treated with respiratory gated radiotherapy from 2015 February to March. Using RPM respiratory gating (RPM 1.7.5, Varian, USA) equipment, imaging according to respiratory cycle of phase based sorting was acquired and using a treatment planning system (Pinnacle 9.2, Philips, USA) the acquired imaging according to respiratory cycle was used to measure the abdominal movement value by respiratory cycle. The measuring point was the point where the center point of the Marker Block and the body surface met in the 50% phase image and here the coordinate values Lateral, Vertical, Longitudinal (X, Y, Z) were set as reference points, and on the X, Z plane identical to the reference point, using the identical method the Y axis coordinate value of each 0%, 30%, 40%, 50%, 60%, 80% phase images were acquired to quantitatively measure the variation of distance to the Y axis. The abdominal movement value according to respiration was applied to the theoretical model that the value decreases linearly from maximum inhalation to maximum exhalation to divide the variation of my value to predict as amplitude value by respiratory cycle and conversely the variation in amplitude was recalculated with the phase variation deviation value to analyze. Results : The deviation value between expected value and actual location was the largest in the 30% phase with 0.24 cm, and standard deviation was also the largest in 30% phase with 0.13 cm. The effective value of the deviation value derived from the average of the deviation squared value of each patient appeared as minimum 0.7 cm, maximum 0.18 cm, average 0.12 cm, and standard deviation 0.4 cm. Also by dividing the actual movement distance value with the peak expiration value then converting it into %Phase, the deviation value with actual phase 16.5% in 30% phase, 10.0% and 40% phase, 10.0% and 60% phase, 15.4% and 80% phase, and overall average about 13%, and arraying based on amplitude, phase shift occurred and further it was from peak expiration the chance of deviation occurrence was increasingly measured. Conclusion : Based on the results of the study there were differences between value acquired based on theoretical model and actual value. Therefore in respiratory gated radiotherapy using external surrogates, there needs to be establishment of respiration gated radiation system that avoids the combination of two Sorting methods considering that there will be occurrence of treatment and corresponding clinical differences due to the phase difference that occur due to the Amplitude based Phase Sorting.

• Radiation Oncology Journal
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• v.23 no.3
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• pp.169-175
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• 2005

Purpose: Although computed tomography (CT) simulators are commonly used in radiation therapy department, many Institution still use conventional CT for treatments. In this study the setup errors that occur during simulation, CT scan (diagnostic CT scanner), and treatment were evaluated for the twenty one breast cancer patients. Materials and Methods: Errors were determined by calculating the differences in isocenter location, SSD, CLD, and locations of surgical clips implanted during surgery. The anatomic structures on simulation film and DRR image were compared to determine the movement of isocenter between simulation and CT scan. The isocetner point determined from the radio-opaque wires placed on patient's surface during CT scan was moved to new position if there was anatomic mismatch between the two images Results: In 7/21 patients, anatomic structures on DRR Image were different from the simulation Image thus new isocenter points were placed for treatment planning. The standard deviations of the diagnostic CT setup errors relative to the simulator setup in lateral, longitudinal, and anterior-posterior directions were 2.3, 1.6, and 1.6 mm, respectively. The average variation and standard deviation of SSD from AP field were 1.9 mm and 2.3 mm and from tangential fields were 2.8 mm and 3.7 mm. The variation of the CLD for the 21 patients ranged from 0 to 6 mm between simulation and DRR and 0 to 5 mm between simulation and treatment. The group systematic errors analyzed based on clip locations were 1.7 mm in lateral direction, 2.1 mm in AP direction, and 1.7 mm in SI direction. Conclusion: These results represent that there was no significant differences when SSD, CLD, clips' locations and isocenter locations were considered. Therefore, it is concluded that when a diagnostic CT scanner is used to acquire an image, the set-up variation is acceptable compared to using CT simulator for the treatment of breast cancer. However, the patient has to be positioned with care during CT scan in order to reduce the setup error between simulation and CT scan.

• Economic and Environmental Geology
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• v.39 no.1 s.176
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• pp.83-93
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• 2006

The Kita-Yamato Trough is characterized by a SW-NE trending narrow graben between the Yamato Bank and the Kita-Yamato Bank in the central East Sea/Japan Sea (ES/JS). Core 20EEZ-1 was obtained in the flat summit of a small ridge from the southwest Kita-Yamato Trough. The sedimentation was mainly controlled by the supply of hemipelgic sediments and substantial tephras from explosive volcanic eruptions of the Quaternary volcanoes. The aim of this study is to reconstruct the tephrostratigraphy from the marine sediments collected from the Kita-Yamato Trough and to provide the atmosphere and ocean conditions during the explosive volcanic eruptions. According to the detailed tephrostratigraphy and lithofacies records, the core sediments were deposited during the last marine isotope stage (MIS) 7. The core consists of four lithofacies, idetified as, oxidized mud (OM), crudely laminated mud (CLM) and bioturbated mud (BM), interbedded with coarse-grained tephra (TP). The major element geochemistry and stratigraphic positions of seven tephra layers suggest that they originated from the Aira caldera in Kyushu area among the Japanese islands (AT tephra; 29.24 ka), unknown submarine volcano in the south Korea Plateau (SKP-I; MIS 3, SKP-II; MIS 4, SKP-IV; boundary between MIS 6 and MIS 5e, SKP-V; MIS 6, respectively), and the Baegdusan volcano in the Korean Peninsula (B-KY1; ca. 130 ka, B-KY2; ca. 196 ka). The absence of tephras originated trom Ulleung Island in core 20EEZ-l suggest that the tephras had not been transported into the Kita-Yamato Trough by atmosphere conditions during the eruptions. On the other hand, the B-KYI and the B-KY2 tephras derived from the Baegdusan volcano were founded in the Kita-Yamato Trough by a presence of prevailing westerly winds during the eruptions. Furthermore, the SKP tephras were characterized by the transport across the air-water interface, causing quickly thrust of raising eruption plumes from subaqueous explosive eruptions. Surface currents may play an important role in controlling the distribution patterns of the SKP tephras to distal areas. The tephrostratigraphic study in the Kita-Yamato Trough provides the important chronostratigraphic marker horizons and the detailed atmosphere and ocean conditions during the explosive eruptions.

• Journal of the Korean association of regional geographers
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• v.6 no.3
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• pp.153-168
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• 2000

An estuarine marsh is semi -inclosed inlets, located between coastal and terrestrial environment. The sediment transport by river and tide through tidal river and vertical accretion by sediment accumulation are important processes in estuarine marsh. An analysis of the vertical accretion rate at various time scale is important work for understanding and managing coastal environments. The purpose of this study is to determin the spatial characteristics of vertical accretion rate in an estuarine marsh, Sunchon Bay, in the southern coastal region of Korean peninsula. The methods of analysis are sedimentation rate by individual tidal cycle, annual accretion rate, concentration of total suspended load in water column. Spatial characteristics of sedimentation rate by individual tidal cycle was investigated using 30 filter paper traps. Sedimentation rate by individual tidal cycle at levee edge was higher than that at back marsh. The sedimentation rate decreased with distance from estuarine front. Levee effect and proximity to the turbidity maximum zone result in a higher sedimentation rate in the levee edge. There is a weak relation-ships between tidal regime and sedimentation rate by individual tidal cycle. Spatial cahracteristics of annual accretion rate was investigared using 30 artificial marker plots. Annual accretion rate at back marsh($1.5{\sim}3.5cm/yr$) was higher than that at tidal river levee edge($0.8{\sim}3.0cm/yr$). Total suspended load (TSL) concentrations in water column also indicate this spatial characteristics of annual accretion rate. TSL concentration in water column leaving the vegetation part dramatically decreased. There is a very strong relationship between the concentration of suspended load and accretion rate. These results indicate that annual accretion rate is controlled by vegetation cover and proximity to the turbidity maximum zone. This difference of spatial characteristics of vertical accretion rate ar various time-scale was due to the fact that surface sediment of levee edge was eroded by tide and other factors. The major findings are as follows. First, the spatial characteristics of vertical accretion rate are different from various time-scale. Second, the major mechanism for the vertical accretion rate in this region is suspended load trapping by vegetation. Third, this region is primarily a depositional regime over the time-scale of the present data Fourth, this estuarine marsh is accreting at rates beyond other area.