• Fisheries and Aquatic Sciences
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• 제9권1호
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• pp.30-37
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• 2006

We performed experiments on the uptake and excretion of dissolved organic phosphorus (DOP) using two toxic dinoflagellates, Alexandrium tamarense Lebour (Balech) and Gymnodinium catenatum Graham, isolated from Hiroshima Bay, Japan. ATP (adenosine triphosphate), UMP (uridine-5-monophosphate), G-6-P (glucose-6-phosphate) and Glycero-P (glycerophosphate) were used as DOP sources in preliminary uptake experiments. ATP was selected as the DOP species for the short-tenn uptake experiment because preliminary experiments showed it to be the DOP source used by both species. Although the $K_s$ values of A. tamarense and G. catenatum (5.63 and $7.61{\mu}M$, respectively) obtained from the short-term experiments for ATP were only slightly higher than those reported for dissolved inorganic phosphorus (DIP), the ${\rho}_{max}$ values (5.04 pmol/cell/h and 13.4 pmol/cell/h, respectively) were much higher. The DOP excretion rate in batch-culture experiments was estimated at 0.084 pmol/cell/h for A. tamarense and 0.012 pmol/cell/h for G. catenatum, accounting for about 30% and 25%, respectively, of the assimilated phosphorus. Our results suggest that the DIP-depleted conditions of Hiroshima Bay favor these two species by supporting their ability to use DOP.

• IMMUNE NETWORK
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• 제22권5호
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• pp.38.1-38.24
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• 2022

Exosomes, which are well-known nanoscale extracellular vesicles, are multifunctional biomaterials derived from endosomes and perform various functions. The exosome is a critical material in cell-cell communication. In addition, it regulates the pathophysiological conditions of the tumor microenvironment in particular. In the tumor microenvironment, exosomes play a controversial role in supporting or killing cancer by conveying biomaterials derived from parent cells. Innate immunity is a crucial component of the host defense mechanism, as it prevents foreign substances, such as viruses and other microbes and tumorigenesis from invading the body. Early in the tumorigenesis process, the innate immunity explicitly recognizes the tumor via Ags and educates the adaptive immunity to eliminate it. Recent studies have revealed that exosomes regulate immunity in the tumor microenvironment. Tumor-derived exosomes regulate immunity against tumor progression and metastasis. Furthermore, tumor-derived exosomes regulate polarization, differentiation, proliferation, and activation of innate immune cells. Exosomes produced from innate immune cells can inhibit or support tumor progression and metastasis via immune cell activation and direct cancer inhibition. In this study, we investigated current knowledge regarding the communication between tumor-derived exosomes and innate immune cell-derived exosomes (from macrophages, dendritic cells, NK cells, and neutrophils) in the tumor microenvironment. In addition, we discussed the potential development of exosomal immunotherapy using native or engineered exosomes against cancer.

• 한국수소및신에너지학회논문집
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• 제20권4호
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• pp.309-316
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• 2009

The effect of gas diffusion layers (GDLs) on the freeze/thaw condition durability in polymer electrolyte fuel cells (PEFCs) were investigated. For this purpose, three kinds of GDLs, such as, felt, paper and cloth types with different basic properties have been first prepared, then the changes in the properties and performance of cells was observed during the freeze/thaw cycles ranging from -30 to $70^{\circ}C$. The performance evaluations were conducted by using the single cells consisting of different GDLs. The performance degradation and the cell resistance increase could be directly correlated. The physical destruction of electrode was shown by SEM analysis. The mechanically supporting ability on the interface between the cell components can help enhancing the durability of PEFCs in the freeze/thaw condition.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 추계종합학술대회 논문집(1)
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• pp.255-258
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• 2000

This paper presents an Interprocessor Communication Network(IPC net) in ATM switching system. In order to supply stable and independent path for processor communication, additional network i.e., Ethernet, is suggested. An Ethernet switch centered on Ethernet binds each processor into a work range. IPC net proposed in this paper assures end-to-end inter-processor connection, uniform 100Mbps Ethernet bandwidth and enhanced user cell throughput of ATM switch with minimum Ethernet supporting block integrated into ATM system

• 한국생산제조학회지
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• 제22권2호
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• pp.281-286
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• 2013

As the product lifecycle is getting shorter and various models should be released to respond to the needs of customers and markets, automation-based flexible production line has been recognized as the core competitiveness. According to these trends, system vendors supply cell-level systems such as FMC(Flexible Manufacturing Cell) that is integration of core functions of FMS(Flexible Manufacturing System) and RMC(Reconfigurable Manufacturing Cell) that can easily extend components of FMC. In the cell-based environment, flexible management for shop floor composed of existing job shop, FMCs and RMCs from various system vendors has emerged as an important issue. However, there could be some problems on integrated operation between heterogeneous cells to use vendor-specific cell controllers and on seamless information flow with high level systems such as ERP(Enterprise Resource Planning). In this context, this paper proposes process management systems supporting integrated shop operation of heterogeneous multi-cell based flexible manufacturing environment: First of all, (1) Integrated Shop Operation System to apply the process management system is introduced, and (2) Multi-Layer BOP(Bill-Of-Process) model, a backbone of the process management system, is derived with its data structure. Finally, application of the proposed model is illustrated through system implementation results.

• 중소기업융합학회논문지
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• 제6권3호
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• pp.21-27
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• 2016

본 논문에서는 스몰셀 통신 환경에서 셀 간 간섭을 완화 시키고, 사용자 수용량을 증가시키기에 적합한 PB/MC-CDMA (Partial Block/MC-CDMA) 시스템을 소개한다. 5G 무선통신 환경에서는 IoT (Internet of Things) 서비스의 확장과 더불어 네트워크에 접속되는 디바이스의 수가 기하급수적으로 증가될 것으로 예상되며, 각 디바이스들은 콘텐츠 별로 다양한 전송속도가 요구 될 것으로 예상된다. 기존의 LTE/LTE-A 통신 서비스 환경에서는 데이터 전송속도와 주파수 효율성 향상을 중심으로 기술 연구가 진행 되었으나, IoT (Internet of Things)에서 요구하는 대규모 접속 및 다양한 전송속도 지원에는 한계성을 지니고 있다. 또한, 다수의 스몰셀 환경에서 셀간 간섭이 발생하여 속도저하 및 시스템 성능 열화가 발생한다. 이에 본 논문에서는 스몰셀에 적합한 PB/MC-CDMA 물리계층 기술을 소개하고, 셀간 간섭 존재하는 다중 셀 환경에서 시스템의 성능을 평가함으로써 IoT (Internet of Things) 환경에서 PB/MC-CDMA의 장점을 검증하였다.

• BMB Reports
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• 제56권9호
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• pp.482-487
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• 2023

Hematopoiesis is regulated by crosstalk between long-term repopulating hematopoietic stem cells (LT-HSCs) and supporting niche cells in the bone marrow (BM). Here, we describe the role of KAI1, which is mainly expressed on LT-HSCs and rarely on other hematopoietic stem-progenitor cells (HSPCs), in niche-mediated LT-HSC maintenance. KAI1 activates TGF-β1/Smad3 signal in LT-HSCs, leading to the induction of CDK inhibitors and inhibition of the cell cycle. The KAI1-binding partner DARC is expressed on macrophages and stabilizes KAI1 on LT-HSCs, promoting their quiescence. Conversely, when DARC+ BM macrophages were absent, the level of surface KAI1 on LT-HSCs decreases, leading to cell-cycle entry, proliferation, and differentiation. Thus, KAI1 acts as a functional surface marker of LT-HSCs that regulates dormancy through interaction with DARC-expressing macrophages in the BM stem cell niche. Recently, we showed very special and rare macrophages expressing α-SMA+ COX2+ & DARC+ induce not only dormancy of LT-HSC through interaction of KAI1-DARC but also protect HSCs by down-regulating ROS through COX2 signaling. In the near future, the strategy to combine KAI1-positive LT-HSCs and α-SMA/Cox2/DARC triple-positive macrophages will improve the efficacy of stem cell transplantation after the ablative chemo-therapy for hematological disorders including leukemia.

• Journal of Microbiology
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• 제41권3호
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• pp.175-182
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• 2003

Production of ribosomally synthesized antimicrobial peptides usually referred to as bacteriocins is an inducible trait in several gram positive bacteria, particularly in those belonging to the group of lactic acid bacteria. In many of these organisms, production of bacteriocins is inducible and induction requires secretion and extracellular accumulation of peptides that act as chemical messengers and trigger bacteriocin production. These inducer peptides are often referred to as autoinducers and are believed to permit a quorum sensing-based regulation of bacteriocin production. Notably, the peptides acting as autoinducers are dedicated peptides with or without antimicrobial activity or the bacteriocins themselves. The autoinducer-dependent induction of bacteriocin production requires histidine protein kinases and response regulator proteins of two-component signal transduction systems. The current working model for the regulation of class II bacteriocin production in lactic acid bacteria and the most relevant direct and indirect pieces of evidence supporting the model are discussed in this minireview.

• BMB Reports
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• 제48권1호
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• pp.48-53
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• 2015

Oxidized LDL (oxLDL) performs critical roles in atherosclerosis by inducing macrophage foam cell formation and promoting inflammation. There have been reports showing that oxLDL modulates macrophage cytoskeletal functions for oxLDL uptake and trapping, however, the precise mechanism has not been clearly elucidated. Our study examined the effect of oxLDL on non-muscle myosin heavy chain IIA (MHC-IIA) in macrophages. We demonstrated that oxLDL induces phosphorylation of MHC-IIA (Ser1917) in peritoneal macrophages from wild-type mice and THP-1, a human monocytic cell line, but not in macrophages deficient for CD36, a scavenger receptor for oxLDL. Protein kinase C (PKC) inhibitor-treated macrophages did not undergo the oxLDL-induced MHC-IIA phosphorylation. Our immunoprecipitation revealed that oxLDL increased physical association between PKC and MHC-IIA, supporting the role of PKC in this process. We conclude that oxLDL via CD36 induces PKC-mediated MHC-IIA (Ser1917) phosphorylation and this may affect oxLDL-induced functions of macrophages involved in atherosclerosis.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 춘계학술대회
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• pp.178-179
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• 2003

The testis is composed of four cell types like supporting cells, steroid-producing cells, connective tissue cells and germ cells. Apoptosis is a common phenomenon during spormatogenesis. Apoptosis of germ cells can also be induced by exposure to radiation. Previous studies have shown that most types of germ cells are rather radiosensitive while somatic cells in testis are much more radio-resistant. The somatic cells in testis are divided to mainly Sertoli and Leydig cells. Though somatic cells are more radio-resistant than germ cells, radiation can induce the impairment of their function. This damaged function of somatic cells may accelerates degeneration of germ cell indirectly. Tn the present study, we have examined the apoptotic effect of mouse testis and irradiation effect of steroidogenesis of Leydig cells after irradiation.