• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.10a
• /
• pp.77-77
• /
• 2003

Coculture of HSC with bone marrow-derived mesenchymal stem cells (BM-MSCs) is one of used methods to increase cell numbers before transplant to the patients. However, because of difficulties to purify HSCs after coculture with BM-MSCs, it needs to develop a method to overcome the problem. In the present study, we have examined whether a culture insert placed over a feeder layer might support the expansion of HSCs within the insert. $CD34^+/ $ cells isolated from the umbilical cord blood by using midiMACS were divided into three groups. A group of 1 $\times$ $10^5$ cells were grown on a culture insert without feeder layer (Direct). The same number of HSCs was directly cocultured with BM-MSCs (Contact). The third group was placed onto an insert below which BM-MSCs were grown (Insert). To distinguish feeder cells from HSCs, BM-MSCs was pre-labeled fluorescently with PKH26 and 1 $\times$ $10^5$ cells were seeded in the culture dishes. After culture for 13 days, the expansion factor (x) of HSCs that were grown without feeder layer (Direct) was $26.6 \pm 8.4.$ In contrast, the number of HSCs directly cocultured with feeder layer was 59.6 $\pm$ 0.5 and that of HSCs cultured onto an insert was $46.9 \pm 8.4.$ The percentage of BM-MSCs cells remained being fluorescent was $97.9 \pm 0.3%$ after culture. Immune-phenotypically large proportion of cultured cells were founded to be differentiated into myeloid/monocyte progenitor cells. The ability of BM-MSCs, fetal lung, cartilage and brain tissue cells to support ex vivo expansion of HSCs was also examined using the insert. After 11 days of coculture with each of these cells, the expansion factor of HSCs was 15.0, 39.0, 32.0 and 24.0, respectively. Based upon these observations, it is concluded that the coculture method using insert is very effective to support ex vivo expansion of HSCs and to eliminate the contamination of other cells used to coculture wth HSCs.

• Journal of the Korean Association of Geographic Information Studies
• /
• v.19 no.2
• /
• pp.98-106
• /
• 2016

Remote sensing is an efficient technology for observing and monitoring the land surfaces inaccessible to humans. This research proposes a methodology for improving the accuracy of the land cover classification using the Landsat-8 operational land imager(OLI) image. The proposed methodology consists of the following steps. First, the normalized difference vegetation index(NDVI) and normalized difference water index(NDWI) images are generated from the given Landsat-8 OLI image. Then, a new image is generated by adding both NDVI and NDWI images to the original Landsat-8 OLI image using the layer-stacking method. Finally, the maximum likelihood classification(MLC), and support vector machine(SVM) methods are separately applied to the original Landsat-8 OLI image and new image to identify the five classes namely water, forest, cropland, bare soil, and artificial structure. The comparison of the results shows that the utilization of the layer-stacking method improves the accuracy of the land cover classification by 8% for the MLC method and by 1.6% for the SVM method. This research proposes a methodology for improving the accuracy of the land cover classification by using the layer-stacking method.

• Membrane Journal
• /
• v.24 no.6
• /
• pp.453-462
• /
• 2014

Thin film composite (TFC) polyamide (PA) membranes were prepared on polyester (PET) nonwoven reinforced polysulfone supports for forward osmosis (FO) processes. PSF (polysulfone) supports were prepared via the phase inversion process from PSF casting solutions in dimethyl formamide (DMF) solvents (19 wt%) by using a PET nonwoven (thickness of $100{\mu}m$) as a mechanical reinforcing material for reverse osmosis (RO) membrane. The PSF support from 19 wt% of DMF/PSF casting solution showed sponge-like morphology and asymmetric internal structure. To reduce the internal concentration polarization in FO operation, thin ($20{\mu}m$ of thickness) nonwoven-supported PSF supports were prepared by using PSF/DMF casting solution (9~19 wt%). A desirable support structure with a highly porous sponge-like morphology were achieved from the thin nonwoven-supported PSF layer prepared with 9~12 wt% casting solution. A crosslinked aromatic polyamide layer was fabricated on top of each support to form a TFC PA membrane. The tested sample from 12 wt% of DMF/PSF casting solution presented outstanding FO performance, almost 5.5 times higher water flux (24.3 LMH) with low reverse salt flux (RDF, 1.5 GMH) compared to a thick nonwoven rainforced membrane (4.5 LMH of flux and 3.47 GMH of RSF). By reducing the thickness of the nonwoven and optimizing PSF concentration of casting solution, the morphology of the prepared membranes were changed from a dense structure to a porous sponge structure in the boundary area between nonwoven and PET support layer.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.43 no.7
• /
• pp.1025-1035
• /
• 2014

This study assessed the effect of immobilized lipase on weak base styrene resin using polyethyleneimine (PEI) with cross-linking. Two procedures were used in this study. The first one, "mono-layer" lipase immobilization, involves washing PEI after adsorption. The second procedure, "multi-layer" lipase immobilization, has no washing before the cross-linking step. Treverlite XS-100200 (weak base styrene resin) was immersed with PEI solution (2.2 mg/mL). Lipase AH (from Burkholderia cepacia) was adsorbed onto the support coated with PEI before cross-linking with glutaraldehyde. Structured lipid was synthesized by immobilized lipase-catalyzed interesterification using canola oil, palmitic ethyl ester (PEE), and stearic ethyl ester (StEE). Total fatty acid contents of triacylglycerol (TAG) in structured lipids were analyzed to investigate activity, properties, and reusability of immobilized lipases. Activities of immobilized lipases on the multi-layer and mono-layer increased at a high concentration (8 mg/mL) of lipase solution used for immobilization. The results show that immobilized lipase with the mono-layer method at pH 8.0 on resin had the highest total saturated fatty acid content (26.17 area%). Activity of immobilized lipase with the multi-layer method at pH 7.5 on support was lower than that of the mono-layer, but total saturated fatty acid content was 16.79 area% higher than that of lipase AH (15.01 area%).

• Journal of Internet Computing and Services
• /
• v.6 no.6
• /
• pp.85-97
• /
• 2005

In this paper, we construct the traffic information database by using the acquired data from the traffic information devices installed in road network, and, by referring to this database, propose the intersection simulation system which can dynamically manage the real-time traffic flow for each section of road from the intersections, This system consists of hierarchical 3 parts, The lower layer is the physical layer where the traffic information is acquired on an actual road. The traffic flow control framework exists in the middle layer. The framework supports the grouping of intersection, the collection of real-time traffic flow information, and the remote monitoring and control by using the traffic information of the lower layer, This layer is designed by extending the distributed object group framework we developed. In upper layer, the intersection simulator applications controlling the traffic flow by grouping the intersections exist. The components of the intersection application in our system are composed of the implementing objects based on the Time-triggered Message-triggered Object(TMO) scheme, The intersection simulation system considers the each intersection on road as an application group, and can apply the control models of dynamic traffic flow by the road's status. At this time, we use the real-time traffic information collected through inter-communication among intersections. For constructing this system, we defined the system architecture and the interaction of components on the traffic flow control framework which supports the TMO scheme and the TMO Support Middleware(TMOSM), and designed the application simulator and the user interface to the monitoring and the controlling of traffic flow.

• Applied Chemistry for Engineering
• /
• v.17 no.6
• /
• pp.604-608
• /
• 2006

Polymer composites for measuring the radioactive contamination are prepared by coating ZnS(Ag) powders as a scintillator on polysulfone base layer. The composites consist of the active layer for a scintillation reaction with radioactive wastes and the transparent support layer for transmittance of light photons emitted by scintillation in the active layer. The binding of the active layer, including ZnS(Ag), on the support layer is proceeded via coating with polysulfone as a binder, without any extra adhesive. The coating was obtained by either casting via a Doctor Blade as applicator or screen printing. The prepared composites feature a monolithic structure, resulting in the complete adhesion between two layers. The composite prepared by the casting technique using an applicator holds a good detection efficiency in measuring the alpha radionuclide, but its structure becomes fragile because of warping in morphology. On the contrary, the composite prepared by the screen printing shows a good detection capacity as well as a good stability in a mechanical shape.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.4 no.3
• /
• pp.341-357
• /
• 2010

Forward Error Correction (FEC) techniques have been adopted to overcome packet losses and to improve the quality of video delivery. The efficiency of the FEC has been significantly compromised, however, due to the characteristics of the wireless channel such as burst packet loss, channel fluctuation and lack of Quality of Service (QoS) support. We propose herein an Adaptive Cross-layer FEC mechanism (ACFEC) to enhance the quality of video streaming over 802.11 WLANs. Under the conventional approaches, FEC functions are implemented on the application layer, and required feedback information to calculate redundancy rates. Our proposed ACFEC mechanism, however, leverages the functionalities of different network layers. The Automatic Repeat reQuest (ARQ) function on the Media Access Control (MAC) layer can detect packet losses. Through cooperation with the User Datagram Protocol (UDP), the redundancy rates are adaptively controlled based on the packet loss information. The experiment results demonstrate that the ACFEC mechanism is able to adaptively adjust and control the redundancy rates and, thereby, to overcome both of temporary and persistent channel fluctuations. Consequently, the proposed mechanism, under various network conditions, performs better in recovery than the conventional methods, while generating a much less volume of redundant traffic.

• Wind and Structures
• /
• v.30 no.1
• /
• pp.69-83
• /
• 2020

Modelling incompressible, neutrally stratified, barotropic, horizontally homogeneous and steady-state atmospheric boundary layer (ABL) is an important aspect in computational wind engineering (CWE) applications. The ABL flow can be viewed as a balance of the horizontal pressure gradient force, the Coriolis force and the turbulent stress divergence. While much research has focused on the increase of the wind velocity with height, the Ekman layer effects, entailing veering - the change of the wind velocity direction with height, are far less concerned in wind engineering. In this paper, a modified k-ε model is introduced for the ABL simulation considering wind veering. The self-sustainable method is discussed in detail including the precursor simulation, main simulation and near-ground physical quantities adjustment. Comparisons are presented among the simulation results, field measurement values and the wind profiles used in the conventional wind tunnel test. The studies show that the modified k-ε model simulation results are consistent with field measurement values. The self-sustainable method is effective to maintain the ABL physical quantities in an empty domain. The wind profiles used in the conventional wind tunnel test have deficiencies in the prediction of upper-level winds. The studies in this paper support future practical super high-rise buildings design in CWE.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.52 no.2
• /
• pp.35-42
• /
• 2010

In this paper, we propose a ubiquitous Farming Diary System which can support the easy and reliable recording of a farming diary for the certificate on environment-friendly agricultural products by using the USN(Ubiquitous Sensor Network) technologies. By using growth-related data, the system can also control farming facilities remotely and automatically. To achieve this goal, the UFDS(Ubiquitous Farming Diary System) is consisted with 3 layers. The first 'physical layer' can collect data from sensors, cameras and facilities then controls the growth environment based on the analyzed information. The second 'Middle layer' can process and store the data from 'physical layer' to sensor manager, image manager, control manager and diary manager separately. The third 'application layer' can provide growth-related services to users through various applications. The UFDS can recording grow history information automatically and Easily. Besides, the system can make an accurate and reliable farming diary with multimedia information such as motion and sound. Furthermore, environmental information such as temperature, humidity, luminance and soil conditions (soil temperature, soil humidity, soil EC) can be monitored in real-time and the facilities managed in remote sites.

• Proceedings of the KIEE Conference
• /
• 2001.11c
• /
• pp.311-314
• /
• 2001

The objectives of this paper include the development of an agile prototype of SFFS, the $CAFL^{VM}$(Computer Aided fabrication of Lamination for Various Material), which is suitable for the multi-item and small-quantity production and various material fabrication. This paper includes remodeling of the layer slices for the 2D cutting, supplementing information of the layer slices and developing process conditions to fabricate products of various shape. And also includes developing control hardware as well as software by enhancing BOF of the manipulator to 3 degree for the precise 2D cutting. It will generate optimal layer trajectory considering the dynamic characteristics of the laser beam. The system can be used as a competitive agile protype system in terms of various materials, fabrication speed, and accuracy by CAD modeling precise layer slicing, material development, robot path control, and optimization of the support structure.