• 대한한의학회지
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• 제24권1호
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• pp.29-40
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• 2003

Object : This research was performed to investigate the protective effect of Aurantii Immaturus Fructus against ischemic damage using PC12 cells and global ischemia in gerbils. Methods : To observe the protective effect of Aurantii Immaturus Fructus on ischemia damage, viability and changes in activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase and production of malondialdehyde (MDA) were observed after treating PC12 cells with Aurantii Immaturus Fructus during ischemic insult. Gerbils were divided into three groups : a normal group, a 5-min two-vessel occlusion (2VO) group, and an Aurantii Immaturus Fructus administered after 2VO group. The CCAs were occluded by microclip for 5 minutes. Aurantii Immaturus Fructus was administered orally for 7 days after 2VO. The histological analysis was performed at 7 days after the surgery. For histological analysis, the brain tissue was stained with 1% cresyl violet solution. Results : The results showed that 1. Aurantii Immaturus Fructus had a protective effect against ischemia in the CAI area of the gerbil hippocampus 7 days after 5-minute occlusion, 2. In the hypoxia/reperfusion model using PC12 cells, the Aurantii Immaturus Fructus had a protective effect against ischemia in the dose of $0.2{\;}\mu\textrm{g}/ml,{\;}2{\;}\mu\textrm{g}/ml{\;}and{\;}20{\;}\mu\textrm{g}/ml$ 3. Aurantii Immaturus Fructus increased the activities of glutathione peroxidase and catalase, 4. The activity of superoxide dismutase (SOD) was increased by ischemic damage, which might represent self protection. This study suggests that Aurantii Immaturus Fructus has some neuroprotective effect against neuronal damage following cerebral ischemia in vivo with a widely used experimental model of cerebral ischemia in Mongolian gerbils, and it also has protective effects on a hypoxia/reperfusion cell culture model using PCq2 cells. Conclusions : Aurantii Immaturus Fructus has protective effects against ischemic brain damage at the early stage of ischemia.

• 대한한의학회지
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• 제24권1호
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• pp.110-121
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• 2003

Objective : This research was performed to investigate the protective effect of Angelicae Dahuri Radix against ischemic damage using PC12 cells and global ischemia in gerbils. Methods : To observe the protective effect of Angelicae Dahuri Radix on ischemia damage, viability and changes in activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase and production of malondialdehyde (MDA) were observed after treating PC12 cells with Angelicae Dahuri Radix during ischemic insult. Gerbils were divided into three groups : a normal group, a 5-min two-vessel occlusion (2VO) group, and an Angelicae Dahuri Radix administered after 2VO group. The CCAs were occluded by microclip for 5 minutes. Angelicae Dahuri Radix was administered orally for 7 days after 2VO. The histological analysis was performed at 7 days after surgery. For histological analysis, the brain tissue was stained with 1% cresyl violet solution. Results : 1. Angelicae Dahuri Radix has a protective effect against ischemia in the CA1 area of the gerbil hippocampus 7 days after 5-minute occlusion, 2. In the hypoxia/reperfusion model using PC12 cells, Angelicae Dahuri Radix has a protective effect against ischemia in the dose of $0.2\mu\textrm{g}/ml$, $2\mu\textrm{g}/ml$ and $20\mu\textrm{g}/ml$, 3. Angelicae Dahuri Radix increased the activities of glutathione peroxidase and catalase. 4. The activity of superoxide dismutase (SOD) was increased by ischemic damage, which might represent self protection. This study suggests that Angelicae Dahuri Radix has some neuroprotective effect against neuronal damage following cerebral ischemia in vivo with a widely used experimental model of cerebral ischemia in Mongolian gerbils, and it also has protective effects on a hypoxia/reperfusion cell culture model using PC12 cells. Conclusions : Angelicae Dahuri Radix has protective effects against ischemic brain damage at the early stage of ischemia.

• 생명과학회지
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• 제19권4호
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• pp.442-448
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• 2009

대부분의 열대 식물은 chilling에 민감하게 반응한다. 대표적 열대 식물인 벼 잎에 대한 light-chilling 처리와 이 후의 회복기(post-chilling) 동안 일어나는 반응들을 알아보았다. Chilling 시 벼 잎에서의 광합성 효율($F_v/F_m$)은 대조구보다 50% 감소하였고, 상대적으로 $H_2O_2$ 양은 48% 증가하였다. 항산화 효소들 중 SOD와 GR 활성은 chilling과 post-chilling 시 증가하였다. 특히 SOD isoforms의 경우 CuZn-SOD와 Mn-SOD 가 발현된 반면 Fe-SOD는 발현되지 않았다. CAT 활성은 chilling 시 감소하였으며, 반면에 APX는 크게 증가하였다. Chilling 시 CAT의 isoforms의 변화를 보면, CAT-2와 -3의 활성이 감소한 것과 대조적으로 post-chilling 시 이들 isoforms의 활성은 증가하였다. 이처럼 APX와 CAT 활성은 벼 잎이 chilling stress를 겪게 될 때 상반되는 변화를 보여주었다.

• 대한한의학방제학회지
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• 제23권1호
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• pp.111-119
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• 2015

Objectives : The purpose of this study was to investigate the antioxidant activity of water extract of Woogyuyeum (WGY) in Leydig cells. Methods : We investigated the cytoprotective effect of WGY in cultured mouse Leydig cells by MTT assay. Leydig cells treated with WGY were incubated in the presence or absence of 50 μM hydrogen peroxide at 37℃ for 24 h. The protective effects of WGY against hydrogen peroxide-induced oxidative stress, lipid peroxide (LPO), superoxide dismutase (SOD), and catalase activity assays were performed in Leydig cells. Results : As a result, WGY showed no significant cytotoxicity in Leygdig cells. WGY showed cell viability as 103.65% in 5 μg/ml concentrations. The cytotoxicity induced by hydrogen peroxide in Leygdig cells, the antioxidant effects of WGY was increased in 1, 5, 50, 100 ug/ml concentraions. 100 μg/ml concentration of WGY showed maximum antioxidant effects. Treatment of cells with 100 μg/ml WGY significantly reduced the MDA concentration to 0.23 nmoles/mg protein. SOD activity was increased at 1, 100 μg/ml concentration of WGY and catalase activity was significantly increased at 50, 100 μg/ml concentrations of WGY, respectively. Conclusions : In conclusion, WGY has antioxidant activities against hydrogen peroxide-induced oxidative stress in Leydig cells.

• Food Science and Biotechnology
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• 제15권1호
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• pp.143-147
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• 2006

Physiological activities of Korean-grown ginger (KG) and Chinese-grown ginger (CG) extracts were examined. Ginger was extracted with water, and 50 and 100% ethanol, and then nitrite-scavenging activity (NSA), superoxide dismutase (SOD)-like activity, and electron-donating ability (EDA) of extracts were investigated. NSA at pH 1.2 was the most effective in 50% ethanol extracts of both origins. SOD-like activities of water and 50% ethanol extracts of both samples were 8.66-35.95% lower than those of 1 and 0.1% L-ascorbate solutions. SOD-like activity of KG extracts was higher than that of CG extracts, and water extracts of samples were the highest. EDA of KG extract was higher (22.23-86.95%) than that of CG extract, while both sample extracts showed lower EDA than those of 1 and 0.1% L-ascorbate solutions.

• 환경생물
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• 제31권4호
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• pp.376-382
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• 2013

[6]-Gingerol, a major polyphenol of ginger (Zingiber officinale), exhibits a variety of biological properties including anti-oxidant, anti-inflammatory and anti-cancer activity. However, the radioprotective effect of [6]-gingerol is still unknown. The aim of this study was to investigate the radioprotective effect of [6]-gingerol against radiation-induced cell cytotoxicity and oxidative stress in HepG2 cells. [6]-Gingerol pretreatment attenuated radiation-induced cell cytotoxicity caused by 5Gy (half lethal dose, $LD_{50}$ of HepG2 cells). The measurements of superoxide dismutase (SOD) and catalase (CAT) activity were also performed. The results showed that [6]-gingerol pretreatment reduced increasing SOD and CAT activity after exposure of IR, indicating that [6]-gingerol protected oxidative stress by regulating cellular antioxidant enzyme (SOD and CAT) activity. These findings suggest that [6]-gingerol acts as a radioprotector by attenuating cell cytotoxicity and oxidative stress.

• 대한본초학회지
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• 제24권1호
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• pp.23-33
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• 2009

Objectives : The present study was performed to clarify the antioxidant and anticancer effects of extract of stamens of Nelumbo nucifera and Kaempferol. Methods : Antioxidant effect was measured by DPPH-radical scavenging activity for electron donating ability (EDA), superoxide dismutase (SOD)-like activity for SOD and lipid peroxidation. Anticancer effect was assessed by MTT absorbance for cytotoxicity. Results : Vitamin E, Kaempferol and ethyl acetate extract of stamens of Nelumbo nucifera increased SOD-like activity and DPPH-radical scavenging activity dose-dependently. On the contrary, lipid peroxidation was time-dependently decreased. Furthermore, Kaempferol and ethyl acetate extract of stamens of Nelumbo nucifera significantly decreased the growth rate of C6 glioma cells. Conclusions : These results suggest that ethyl acetate extract of stamens of Nelumbo nucifera may be a putative antioxidant or anticancer substance.

• Journal of Nutrition and Health
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• 제21권5호
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• pp.295-304
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• 1988

High PUFA식이와 vitamin E 함량이 흰쥐 간장내의 지질과산화에 미치는 영향을 알아보기 위하여, 45Kcal% 고불포화 옥수수유의 정상적 vitamin E함량을 가한 식이군(PF군)과 45Kcal% 고불포화 옥수수유의 가한 PF군에 vitamin E를 5배 첨가한 식이군(PFE군), 45Kcal% 고불포화 옥수수유 식이에 vitamin E를 가하지 않은 식이군 (PFO군)을 실험군으로 하여 3주, 6주 및 9주간 사육한 후 간장에서의 vitamin E 함량과 과산화지질량 및 SOD와 GPX활성을 조사하였다. High PUFA식이에 vitamin E를 정상적으로 가한 PF군은 대조군과 차이가 나타나지 않았으나, vitamin E를 충분히 첨가한 식이 PFE군은 식이내 vitamin E 공급이 부족한 PFO군보다 vitamin E 함량과 GPX활성이 증가하였고, SOD활성과 과산화지질향은 현저하게 감소하였다. 식이 투여기간별로 PF군과 PFE군에 비해 PFO군이 실험기간이 경과할수록 vitamin E 함량과 GPX활성이 감소하였으나, SOD활성과 과산화지질향은 현저히 증가하였다.

• Journal of Preventive Medicine and Public Health
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• 제31권3호
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• pp.449-459
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• 1998

This study was started to find out if plasma malondialdehyde(MDA), $\alpha-tocopherol$ and erythrocyte superoxide dismutase(SOD) activity could be markers of biological activity resulting from exposed to lead in workers. Blood samples were randomly obtained from lead-exposed workers(n=29), $CO_2$, welders(n=60) and office workers(n=60). We used whole blood to analyse blood lead with atomic absorption spectrophotometer. Superoxide dismutase activity in erythrocyte was measured with spetrophotometer. MDA and $\alpha-tocopherol$ in plasma were measured with high performance liquid chromatography. Lead-exposed workers was significantly high in blood lead concentration$(29.37{\mu}g/d\ell)$ compared with welders$(6.42{\mu}g/d\ell)$ and office workers$(5.01{\mu}g/d\ell)$. The level of plasma MDA was significantly higher in the lead-exposed workers($1.87{\mu}mol/g$ cholesterol) than the welders($1.41{\mu}mol/g$ cholesterol) and office workers($1.41{\mu}mol/g$ cholesterol). Erythrocyte SOD activity in lead-exposed workers(56.80 U/g Hb) was significantly increased than those of welders(37.63 U/g Hb) and office workers(20.47 U/g Hb). The plasma $\alpha-tocopherol$ level of lead-exposed workers($4.93{\mu}g/g$ cholesterol) was statistically different from welders($4.25{\mu}g/g$ cholesterol) and office workers$4.28{\mu}g/g$ cholesterol). Neither age nor smoking was related to SOD or MDA level. Blood lead was significantly correlated with erythrocyte SOD activity(r=0.405), plasma MDA(r=0.296) and $\alpha-tocopherol$ (r=0.207). Plasma MDA was also significantly correlated with SOD (r=0.217). In multiple regression analysis, the change of MDA and SOD activity level related to the blood lead concentration. These results suggested that the increase of plasma MDA and erythrocyte SOD activity in lead-exposed workers had a close relationship with the oxidative stress induced by lead.

• 한국식품과학회지
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• 제27권3호
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• pp.281-285
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• 1995

감잎차의 효과적인 제조법 및 음용방법을 조사하기 위해 세가지 방법(재래식, 증자법, 발효법)에 의해 감잎으로부터 감잎차를 제조하여 vitamin C와 SOD 유사활성의 변화를 조사하였다. Vitamin C는 발효법에 의해 제조된 감잎차에서 가장 함량이 높았으며 이 수치는(551.85 mg%) 생감잎에 들어 있는 vitamin C의 (1173.67 mg%) 47%에 해당하는 양이며 재래식 및 증자법에 의한 감잎차에 대해 각각 42배, 8배나 되는 많은 양임이 밝혀졌다. 반면 SOD 유사활성은 재래식(833.33 SOD unit/g)과 발효법(816.33 SOD unit/g)에 의해 제조된 감잎차가 증자법(754.72 SOD unit/g)에 비해 조금 높은 것으로 나타났지만 절대 수치에서는 큰 차이가 없었다. 추출온도와 시간에 따라 증자법 감잎차의 vitamin C는 온도에 상관없이 추출시간의 증가에 따라 조금씩 감소하였다. 발효법 감잎차는 $60^{\circ}C$와 $70^{\circ}C$에서는 추출량이 시간의 증가에 따라 대체적으로 증가하였으나 $80^{\circ}C$와 $90^{\circ}C$에서는 추출시간 5분까지는 증가하고 이후에는 감소하였다. 발효법 감잎차의 vitamin C의 최적 추출조건은 $80^{\circ}C$와 $90^{\circ}C$에서 3분과 5분이었고 $70^{\circ}C$에서는 5분과 10분 그리고, $60^{\circ}C$에서는 10분이었다. 재래식 감잎차의 vitamin C는 그 양이 적어 실험에 사용된 DNP법에 의해서는 측정할 수 없었다. SOD유사활성을 살펴볼 때 재래식 및 증자법 감잎차는 추출시간에 따라 $80^{\circ}C$와 $90^{\circ}C$에서 조금씩 증가하는 경향을 나타내었으며 발효법 감잎차는 $80^{\circ}C$와 $90^{\circ}C$에서 증가${\rightarrow}$감소${\rightarrow}$증가하는 경향을 보였고 재래식 및 증자법에 의한 감잎차보다 $80^{\circ}C$와 $90^{\circ}C$ 경우 추출시간에 관계없이 모든 조건에서 높은 활성을 나타냈으며 SOD 유사활성 물질의 최적 추출조건은 3분과 10분이었다.