• Title/Summary/Keyword: Superior genetics

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Genome-Wide Analysis of DNA Methylation before- and after Exercise in the Thoroughbred Horse with MeDIP-Seq

  • Gim, Jeong-An;Hong, Chang Pyo;Kim, Dae-Soo;Moon, Jae-Woo;Choi, Yuri;Eo, Jungwoo;Kwon, Yun-Jeong;Lee, Ja-Rang;Jung, Yi-Deun;Bae, Jin-Han;Choi, Bong-Hwan;Ko, Junsu;Song, Sanghoon;Ahn, Kung;Ha, Hong-Seok;Yang, Young Mok;Lee, Hak-Kyo;Park, Kyung-Do;Do, Kyoung-Tag;Han, Kyudong;Yi, Joo Mi;Cha, Hee-Jae;Ayarpadikannan, Selvam;Cho, Byung-Wook;Bhak, Jong;Kim, Heui-Soo
    • Molecules and Cells
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    • v.38 no.3
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    • pp.210-220
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    • 2015
  • Athletic performance is an important criteria used for the selection of superior horses. However, little is known about exercise-related epigenetic processes in the horse. DNA methylation is a key mechanism for regulating gene expression in response to environmental changes. We carried out comparative genomic analysis of genome-wide DNA methylation profiles in the blood samples of two different thoroughbred horses before and after exercise by methylated-DNA immunoprecipitation sequencing (MeDIP-Seq). Differentially methylated regions (DMRs) in the pre-and post-exercise blood samples of superior and inferior horses were identified. Exercise altered the methylation patterns. After 30 min of exercise, 596 genes were hypomethy-lated and 715 genes were hypermethylated in the superior horse, whereas in the inferior horse, 868 genes were hypomethylated and 794 genes were hypermethylated. These genes were analyzed based on gene ontology (GO) annotations and the exercise-related pathway patterns in the two horses were compared. After exercise, gene regions related to cell division and adhesion were hypermethylated in the superior horse, whereas regions related to cell signaling and transport were hypermethylated in the inferior horse. Analysis of the distribution of methylated CpG islands confirmed the hypomethylation in the gene-body methylation regions after exercise. The methylation patterns of transposable elements also changed after exercise. Long interspersed nuclear elements (LINEs) showed abundance of DMRs. Collectively, our results serve as a basis to study exercise-based reprogramming of epigenetic traits.

Statistical Genetic Studies on Cattle Breeding for Dairy Productivity in Bangladesh: II. Estimation of Reciprocal and Heterosis Effects and Optimum Crossbreeding System between the Local Breeds and Exotic Breeds for Milk Performance

  • Hossain, K.B.;Takayanagi, S.;Miyake, T.;Bhuiyan, A.K.F.H.;Sasaki, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.6
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    • pp.777-782
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    • 2002
  • Reciprocal effects among local breeds and additive and heterosis effects were estimated for total milk production per lactation (TLP), lactation length (LL) and daily milk yield (DMY). Procedures for an optimum crossbreeding system were examined by comparing various crossbred combinations. Three data sets were chosen from the total records collected from 1962 to 1997. Data set I with a total of 5,938 records composed of 9 breed groups for reciprocal crosses, data set II with a total of 6,064 records composed of 5 breed groups for additive and heterosis effects and data set III with a total of 10,319 records composed of 42 breed groups for optimum crossbreeding system were analyzed. The least squares ANOVA was used. The reciprocal crosses between Red Sindhi and the Bangladeshi Local showed significant difference. When the Red Sindhi was used as cow, the cross was superior to the opposite in terms of the three performance traits. The results showed that additive breed effects on both TLP and DMY were positive and highly significant between Bos indicus and Bos taurus, whereas the individual heterosis effects were not significant. In general, any local breeds were improved two to three times by crossing with the Friesian once or twice. However, the effect was reduced when the grading up with the Friesian was repeated more than twice. On the other hand, when the local breed was crossed with the other breeds such as Holstein and Jersey, the improvement of TLP was less. Crossing the F1 cows with the Friesian bull gave further improvement. Almost the same trends were found also for LL and DMY. The local breeds and their crosses are raised mainly for draft animals, then it is recommended that the crossbreds between the local cows and Friesian are used for dairy purposes and more than 75% Friesian blood is used for beef.

Speed Control of Induction Motors using GA based PI Controller

  • Lee, Jae-Do;Lee, Hak-Ju;Oh, Sung-Up;Joo, Hyung-Jun;Seong, Se-Jin
    • Proceedings of the KIPE Conference
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    • 2001.10a
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    • pp.404-408
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    • 2001
  • This paper deals with speed control of induction motors with a gain tuning based on simple Genetic Algorithms, which are search algorithms based on the mechanics of natual selection and genetics. Based on the designed control system structure, the indirect vector control system of induction motors is simulated. The simulation results show that the system has a strong robust to the parameter variation and is insensitive to the load disturbance. Thus, the proposed PI controller based on genetic algorithms is superior to manually tuned classical PI controller in improving the speed control performance of induction motors.

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A Study on the Optimal Design of Laminated Composites using Genetic Algorithm (유전자 알고리즘을 이용한 적층복합재료의 최적설계에 관한 연구)

  • 조석수;주원식;장득열
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1996.04a
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    • pp.729-737
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    • 1996
  • Laminated composite plates have been applied to aircraft structures because their properties are superior to the conventional materials and the laminates have anisortropic elastic properties. However, it tis diffcult to determine stacking structures using actual design variables for the lack of searching capability of existing optimization technique. GA(generic algorithms) are robust search algorithms based on the mechanics of natural selection and natural genetics. Therefore, this study presents an application of IGA to stiffness and weight optimization design and gives the various stacking structures suitable to constraint conditions.

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Hatching Rate of Eggs, and Growth of Larvae and Juveniles from Selected Olive Flounder, Paralichthys olivaceus (선발 육종된 넙치, Paralichthys olivaceus의 부화율 및 자치어 성장)

  • Min, Byung-Hwa;Lee, Jeong-Ho;Noh, Jae-Koo;Kim, Hyun-Chul;Park, Choul-Ji;Choi, Sang-Jun;Myeong, Jeong-In
    • Development and Reproduction
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    • v.13 no.4
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    • pp.239-247
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    • 2009
  • Hatching rate, larval deformation and growth rate of selected olive flounder (Paralichthys olivaceus) for rapid growth were compared to those of the unselected fish. Fish were spawned on the same day and cultured under the similar conditions. The selected fish had a significantly higher eggs hatching rate, and lower larval deformation. The selected fish grew significantly faster, and at the end of the experiment (8 weeks after hatching) averaged 50.49${\pm}$2.67 mm in total length, 16.30${\pm}$0.08 mm in body height, and 1.036${\pm}$0.118 g in weight, compared to 40.55${\pm}$3.13 mm, 13.50${\pm}$0.96 mm, and 0.557${\pm}$0.073 g for unselected fish, respectively. The selected fish had a significantly higher body shape index, however lower condition factor than the unselected fish. The results of the present study demonstrate that the selected fish of the olive flounder for rapid growth had superior growth and improved body shape.

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Prediction of 305 Days Milk Production from Early Records in Dairy Cattle Using an Empirical Bayes Method

  • Pereira, J.A.C.;Suzuki, M.;Hagiya, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.11
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    • pp.1511-1515
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    • 2001
  • A prediction of 305 d milk production from early records using an empirical Bayes method (EBM) was performed. The EBM was compared with the best predicted estimation (BPE), test interval method (TIM), and the linearized Wood's model (LWM). Daily milk yields were obtained from 606 first lactation Japanese Holstein cows in three herds. From each file of 305 daily records, 10 random test day records with an interval of approximately one month were taken. The accuracies of these methods were compared using the absolute difference (AD) and the standard deviation (SD) of the differences between the actual and the estimated 305 d milk production. The results showed that in the early stage of the lactation, EBM was superior in obtaining the prediction with high accuracy. When all the herds were analyzed jointly, the AD during the first 5 test day records were on average 373, 590, 917 and 1,042 kg for EBM, BPE, TIM, and LWM, respectively. Corresponding SD for EBM, BPE, TIM, and LWM were on average 488, 733, 747 and 1,605 kg. When the herds were analyzed separately, the EBM predictions retained high accuracy. When more information on the actual lactation was added to the prediction, TIM and LWM gradually achieved better accuracies. Finally, in the last period of the lactation, the accuracy of both of the methods exceeded EBM and BPM. The AD for the last 2 samples analyzing all the herds jointly were on average 141, 142, 164, and 214 kg for LWM, TIM, EBM, and BPE, respectively. In the current practices of collecting monthly records, early prediction of future milk production may be more accurate using EBM. Alternatively, if enough information of the actual lactation is accumulated, TIM may obtain better accuracy in the latter stage of lactation.

Heritabilities of Growth and Wood Quality Characters in Open-pollinated Progenies of Abies holophylla Max. (전나무 수형목(秀型木)의 풍매차대(風媒次代)에 대한 생장(生長)과 재질특성(材質特性)의 유전력(遺傳力))

  • Ahn, Jin Kwon;Lee, Wi Young;Jo, Dong Gwang;Son, Doo Sik
    • Journal of Korean Society of Forest Science
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    • v.83 no.4
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    • pp.480-485
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    • 1994
  • Growth and wood quality characters such as height, DBH, specific gravity, traceried length and width were measured for 16-year-old open-pollinated progenies of 38 selected plus trees of Abies holophylla Max. The results were as follows ; 1) The superior families in growth and wood quality characters were Chonbuk 4(498.2cm) and Kyonggi 9(485.7cm) for height, Kyonggi 12(9.2cm) and 9(9.1cm) for DBH, Kaongbuk 2(0.480) and Kangwon 7(0.473) for specific gravity, Kyonggi 9(2.77mm) and Kangwon 11(2.71mm) for tracheid length and Kyonggi 9(0.039mm), Kangwon 11, 12 and Kyunggi 7(0.036mm) for tracheid width, respectively. 2) There were significant differences among the families in height and specific gravity at 1% and 5% level. 3) There were high significant negative correlations between specific gravity and other characters such as height, DBH, tracheid length and width at 1% level($r=-0.242^{**}{\sim}-0.635^{**}$) 4) The heritabilities were 0.55 for height, 0.25 for DBH, 0.44 for specific gravity, and 0.24 for tracheid width.

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Evaluation of coat color inheritance and production performance for crossbreed from Chinese indigenous Chenghua pig crossbred with Berkshire

  • Li, Yujing;Yuan, Rong;Gong, Zhengyin;Zou, Qin;Wang, Yifei;Tang, Guoqing;Zhu, Li;Li, Xuewei;Jiang, Yanzhi
    • Animal Bioscience
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    • v.35 no.10
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    • pp.1479-1488
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    • 2022
  • Objective: This work was to determine coat inheritance and evaluate production performance for crossbred pigs from Berkshire×Chenghua (BC) compared with Chinese indigenous Chenghua (CH) pigs. Methods: The coat color phenotypes were recorded for more than 16,000 pigs, and the genotypes of melanocortin 1 receptor (MCIR) gene were identified by sequencing. The reproductive performance of 927 crossbred BC F4 gilts and 320 purebred CH gilts was recorded. Sixty pigs of each breed were randomly selected at approximately 60 days of age to determine growth performance during fattening period, which lasted for 150 days for BC pigs and 240 days for CH pigs. At the end of the fattening period, 30 pigs of each breed were slaughtered to determine carcass composition and meat quality. Results: The coat color of BC pigs exhibits a "dominant black" hereditary pattern, and all piglets derived from boars or sows genotyped ED1 ED1 homozygous for MC1R gene showed a uniform black coat phenotype. The BC F4 gilts displayed a good reproductive performance, showing a higher litter and tear size and were heavier at farrowing litter and at weaning litter than the CH gilts, but they reached puberty later than the CH gilts. BC F4 pigs exhibited improved growth and carcass characteristics with a higher average daily live weight gain, lower feed-to-gain ratio, and higher carcass lean meat rate than CH pigs. Like CH pigs, BC F4 pigs produced superior meat-quality characteristics, showing ideal pH and meat-color values, high intramuscular fat content and water-holding capacity, and acceptable muscle-fiber parameters. C18:1, C16:0, C18:0, and C18:2 were the main fatty acids in M. longissimus lumborum in the two breeds, and a remarkably high polyunsaturated/saturated fatty acid ratio of ~0.39 was observed in the BC F4 pigs. Conclusion: The BC F4 pigs exhibit a uniform black coat pattern and acceptable total production performance.

The relationship between 3D bone architectural parameters and elastic moduli of three orthogonal directions predicted from finite elements analysis (돼지 하악 과두의 해면골에서 유한요소분석법으로 예측한 방향에 따른 탄성율과 3차원 골 미세지표 간의 상관관계)

  • Park, Kwan-Soo;Lee, Sam-Sun;Huh, Kyung-Hoe;Yi, Won-Jin;Heo, Min-Suk;Choi, Soon-Chul
    • Imaging Science in Dentistry
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    • v.38 no.2
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    • pp.81-87
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    • 2008
  • Purpose: To investigate the relationship between 3D bone architectural parameters and direction-related elastic moduli of cancellous bone of mandibular condyle. Materials and Methods: Two micro-pigs (Micro-$pig^R$, PWG Genetics Korea) were used. Each pig was about 12 months old and weighing around 44 kg. 31 cylindrical bone specimen were obtained from cancellous bone of condyles for 3D analysis and measured by micro-computed tomography. Six parameters were trabecular thickness (Tb. Th), bone specific surface (BS/BV), percent bone volume (BV/TV), structure model index (SMI), degree of anisotropy (DA) and 3-dimensional fractal dimension (3DFD). Elastic moduli of three orthogonal directions (superior-inferior (SI), medial-lateral (ML), andterior-posterior (AP) direction) were calculated through finite element analysis. Results: Elastic modulus of superior-inferior direction was higher than those of other directions. Elastic moduli of 3 orthogonal directions showed different correlation with 3D architectural parameters. Elastic moduli of SI and ML directions showed significant strong to moderate correlation with BV/TV, SMI and 3DFD. Conclusion: Elastic modulus of cancellous bone of pig mandibular condyle was highest in the SI direction and it was supposed that the change into plate-like structure of trabeculae was mainly affected by increase of trabeculae of SI and ML directions.

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Ethnobotany of Wild Baobab (Adansonia digitata L.): A Way Forward for Species Domestication and Conservation in Sudan

  • Gurashi, N.A.;Kordofani, M.A.Y.;Adam, Y.O.
    • Journal of Forest and Environmental Science
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    • v.33 no.4
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    • pp.270-280
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    • 2017
  • Selection of superior phenotypes of fruit trees and products based on established criteria by local people is a prerequisite for future species domestication and conservation. Thus the study objective was to identify the local people's perceptions and preferences on baobab trees and products. A sample of 142 respondents was randomly selected using structured interviews in Blue Nile and North Kordofan, Sudan in 2013. Descriptive analysis was employed using SPSS and Excel programs. The study results indicated that local people use the morphological characteristics of the tree (leaves, fruits, seeds, kernels and bark) to differentiate individual trees. Based on the perceptions, local people recorded trees with delicious leaves, white pulp color, big fruit size and mature capsule size, and high pulp yield as criteria for differentiating between baobab trees in the study areas. In contrast, the undesirable traits were connected to trees with acidic pulp, slimy pulp, bitter leaves, and low pulp yield. The study concluded that the ethnobotanical knowledge of the baobab tree and its products may play an important role in tree domestication and improvement in Sudan. However, further research on tree genetics is needed to complement the ethnobotanical knowledge for baobab resources domestication and conservation.