• Title/Summary/Keyword: Substrate loss

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Segregational Instability of a Recombinant Plasmid pDML6 in Streptomyces lividans

  • LEE, JUNG HYUN;JAE DEOG JANG;KYE JOON LEE
    • Journal of Microbiology and Biotechnology
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    • v.2 no.2
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    • pp.129-134
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    • 1992
  • Segregational instability of a recombinant plasmid, pDML6, encoding extracellular $\beta$-lactamase in Streptomyces lividans PD6 was characterized by growth kinetic analysis. The quantitative determination of the plasmid harbored in the mycelia was evaluated with mycelia fragmented mechanically, and also with colonies regenerated from protoplasts. Conditions for the formation of protoplasts and regeneration of protoplasts were established. The maximal specific growth rates of the host strain and the plasmid-harboring strain in a chemically defined medium without selection pressure were the same. The probability of plasmid loss from the harbouring cells was higher at higher growth rates. Mathematical models for the prediction of cell growth, substrate uptake, and accumulation of the cloned gene product were developed.

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Electrical Characteristics of ($Sr_{0.85}Ca_{0.15})TiO_3$ Thin Film by RF Sputtering Method (RF 스퍼터링법에 의한 ($Sr_{0.85}Ca_{0.15})TiO_3$ 박막의 전기적 특성)

  • 장원석;김진사;최운식;김충혁;이준웅
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1998.11a
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    • pp.239-242
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    • 1998
  • The (S $r_{0.85}$C $a_{0.15}$)Ti $O_3$ (SCT) thin films are deposited on Pt-coated electrode using RF magnetron sputtering method at various substrate temperature. Dielectric constant of SCT thin films is increased with increased as the deposition temperature and changes almost linearly in temperature ranges from -80 to +90[$^{\circ}C$]. The drastic decrease of dielectric constant and increase of dielectric loss in SCT thin films is observed above 200[kHz]. V-I characteristics of SCT thin films show the increasing leakage current with the increases of deposition temperature.ure.

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Structural and Dielectric Properties of PZT(10/90)/PZT(90/10) Heterolayered Thin Films Prepared by Sol-Gel Method (Sol-Gel법으로 제작한 PZT(10/90)/PZT(90/10)이종층 박막의 구조 및 유전특성)

  • 김경태;정장호;박인길;이성갑;이영희
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1998.11a
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    • pp.247-250
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    • 1998
  • Ferroelectric PZT(10/90)PZT(90/10) heterolayered thin films were fabricated by spin-coating method on the Pt/Ti/SiO$^2$/Si substrate alternately using PZT(10/90) and PZT(90/10) metal alkoxide solutions. The coating and heating procedure was repeated six times to form PZT heterolayered films. The surface, cross-sectional microstructures and thickness of the films were observed using scanning electron microscope(SEM). The relative dielectric constant and dielectric loss of the 5-coated PZT heterolayered films were 1331 and 4.8% respectively.

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Structural and Electrical Properties of (Ba,Sr)$TiO_3$[BST] Thin Films with Ar/$O_2$ ratio (Ar/$O_2$ 비에 따른 (Ba,Sr)$TiO_3$ 박막의 구조 및 전기적 특성)

  • 신승창;이문기;류기원;배선기;이영희
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1998.11a
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    • pp.243-246
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    • 1998
  • (Ba, Sr)TiO$_3$[BST] thin films were fabricated on Pt/SiO$_2$/Si substrate by RF sputtering technique. The structural, dielectric and electrical properties of BST thin films were investigated with Ar/O$_2$ ratio. Dielectric constant and dielectric loss of the BST thin film were about 1020 and 2.0[%], respectively. (at RF power 80W, post annealing temperature $650^{\circ}C$, deposition pressure of 5mTorr and Ar/O$_2$=80/20). For the BST(Ar/O$_2$=80/20) thin film with Polarization switching cycles of 10$^{10}$ , remanent polarization and coercive field were 0.084[$\mu$C/cm$^2$], 1.954[kV/cm], respectively.

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Inhibitory Actions of Mycotoxins on Brain $\gamma$-Aminobutyrate Transaminase ($\gamma$-Aminobutyrate Transaminase에 대한 Mycotoxin의 저해작용)

  • Lee, Su-Jin;Lee, Kil-Soo;Choi, Soo-Young
    • Korean Journal of Microbiology
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    • v.31 no.3
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    • pp.224-229
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    • 1993
  • GABA transminase (4-aminobutyrate aminotransferase), which catalyzes the breakdown of the major inhibitory neurotransmitter, GABA, in mammalian brain, was inactivated by preincubation with the mycotoxin patulin. The time course of the reaction was significantly affected by the substrate .alpha.-ketoglutarate, which aforded complete protection against the loss of catalytic activity. The recovery from the inhibition of patulin by the addition of dithiothreitol (DTT) supports that patulin reacts with the sulfhydryl residue in the catalytic domain of the enzyme. The reconstitution of the reduced enzyme and apoenzyme with pyridoxal-5-P(PLP) was inhibited by another mycotoxin, penicilic acid. This mycotoxin may interact with lysyl residue of the enzyme. Therefore, it is postulated that the critical sulfhydryl and lysyl residues in the catalytic domain of the enzyme react with mycotoxin patulin and penicillic acid, respectively.

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Fabrication of 800 MHz band duplexer using SAW filters (표면탄성파 필터를 이용한 MHz 대역용 듀플렉서 제작)

  • 양형국;조현민;박종철
    • Proceedings of the IEEK Conference
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    • 1998.06a
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    • pp.367-370
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    • 1998
  • A hybrid-integrated SAW(surface acoustic wave) douplexer including transmitter(Tx) filter, receiver(Rx) filter an dantenna terminal has been studied. The prototype duplxer was designed for CDMA system application in the 800 MHz-band. Ladder type SAW filters consisting of one port SAW resonators wer integrated on epoxy substrate to demonstrate the feasiblity of antenna duplexer. In order to perform the duplexing function, a strip line for impedance transformaton with length of .gamma./4 was inserted at rx path. The fabricted duplexer has an insertion loss of 3.0 dB at Tx path and 3.7 dB at Rx path. The attenuation has more than 40dB.

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Properties of Ferroelectric $PbTiO_3$ Thin Films Prepared on ITO/Glass Substrates (ITO/Glass 기판위에 제조된 강유전성 $PbTiO_3$ 박막의 특성)

  • 김승현;오영제;김창은
    • Journal of the Korean Ceramic Society
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    • v.31 no.11
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    • pp.1315-1322
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    • 1994
  • In this study, stable PbTiO3 coating solution was prepared using diethanolamine(DEA) complexing agent and deposited on indium-tin oxide(ITO) coated glass substrate. Prepared thin films were dense and crack-free. Perovskite-type PbTiO3 thin films could be obtained above 50$0^{\circ}C$, while the films heat-treated above $650^{\circ}C$ showed undesired properties due to interface reactions between films and substrates and warpage phenomena of substrates. Measured maximum dielectric constant and loss tangent were found to be 144 and 0.0163 at 1 kHz, 55$0^{\circ}C$ heat-treatment, respectively.

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Synthesis and Characterization of Polyimide Films for Flexible Display Substrates

  • Vu, Quang Hung;Kim, Jin-Woo;Park, Lee-Soon
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.633-636
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    • 2008
  • A series novel films of polyimide (PI) and co-polyimide (Co-PI) containing fluorine with colorless, flexible properties was prepared by a two-step process from various commercial aromatic monomers such as 4,4'-(Hexafluoro iso propylidene) diphthalic anhydride (6FDA), 2,2'-Bis(Trifluoromethyl) benzidine (TFDB), 2,2-bis(3-amino-4-hydroxyphenyl) hexafluoropropane (AH6FP) and Bis(4-(3-aminophenoxy)phenyl)sulfone (BAS). Furthermore, these obtained transparent and flexible Co-PI films exhibited excellent thermal stability with the decomposition temperature (at 5% weight loss) around of $500^{\circ}C$ and the glass transition temperature ($T_g$) in the range of $275-350^{\circ}C$.

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Design of Inset Microstrip Patch Antenna for Wireless Power Transmission at 2.45 GHz

  • Pradhan, Sajina;Noh, Sun-Kuk;Choi, Dong-You
    • Journal of information and communication convergence engineering
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    • v.10 no.2
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    • pp.123-128
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    • 2012
  • In communication systems, there are various types of microstrip antenna that can be used for many applications. This paper mainly focuses on the simple design of an inset rectangular microstrip patch antenna to operate at a frequency of 2.45 GHz for rectenna design. The study involves using an high frequency structure simulator to design the antenna dimensions and to determine its performance. This antenna is based on a thickness of 1.6 mm flame retardant 4 (FR-4) substrate having a dielectric constant of approximately 4.7, an inset feed, and a ground plane. After simulation, the antenna performance characteristics such as its return loss, voltage standing wave ratio, gain, and radiation pattern were obtained and compared with the fabricated measured antenna.

A 20-GHz Miniaturized Ring Hybrid Circuit Using TFMS on Low-Resistivity Silicon

  • Lee Sang-No;Lee Joon-Ik;Yook Jong-Gwan;Kim Yong-Jun
    • KIEE International Transactions on Electrophysics and Applications
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    • v.5C no.2
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    • pp.76-80
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    • 2005
  • In this paper, a miniaturized ring hybrid circuit is characterized based on a thin film microstrip (TFMS) on low-resistivity silicon. In order to obtain low-loss characteristics, a polyimide layer with 50 $\mu$m thickness is spin-coated onto the silicon to be used for the substrate. First, propagation characteristics of TFMS lines consisting of the ring hybrid circuit are presented. Then, a ring hybrid circuit based on TFMS is featured by employing the triple concentric circle approach for miniaturization. Triple concentric circle lines with $\lambda$$_{g}$/4 or 3$\lambda$$_{g}$/4 line lengths are implemented on the surface of the polyimide by circularly meandering to reduce the circuit size of the designed ring hybrid. Good agreement between measured and simulated results is obtained.