• Korean Journal of Plant Resources
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• v.10 no.2
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• pp.107-113
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• 1997

Transformants of White Clover(Trifolium repens L.) were efficiently produced from immature seed derived callus cocultivated with Agrobacterium twnefaciens LBA4404 harboring plant binary vector. pBI121, using acetosyringone. The mean frequencies of transformants on the two kanamycin-containing media were 16 to 19% when the immature seed-derived calli were infected with bacteria cultured in the presence of 100$\mu$M acetosyringone compared with 7% in media without acetosyringone. Transgenic white clover was subject to molecular analysis for integration into plant nuclear genome and expression of $\beta$-glucuronidase(GUS) gene. PCR and Northern blot analyses demonstrated that GUS gene was integrated into white clover nuclear genome and expressed into its mRNA. The expression of GUS gene into its protein was confirmed by spectrophotometric assay of GUS activity.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.25 no.4
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• pp.22-31
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• 2002

A high-speed rail system represents a typical example of large-scale multi-disciplinary systems, consisting of subsystems such as train, electrical hardware, electronics, control, information, communication, civil technology etc. The system design and acquisition data of the large-scale system must be the subject under strict configuration control and management. Not only the requirements of the large-scale system dictate the contracts with the suppliers but also become the basis for the development process, project execution, system integration, and testing. The requirements database provide the system design specification of all development activities. Using the RDD-100, a systems engineering tool, the Korea next-generation high-speed rail program can establish requirements traceability and development process management in performing the enabling train technology development projects. This paper presents the results from a computer-aided systems engineering application to the Korea next-generation high-speed railway project. Especially, the focus of the study was on requirement management and PBS(Product Breakdown Structure) management.

• Korean Journal of Plant Tissue Culture
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• v.21 no.1
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• pp.55-58
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• 1994

Agrobacterium tumefaciens LABA4404 harboring plant binary vector, pBI121, was used for genetic transformation of lettuce (Lactuca sativa t.). Cotyledon segments were infected with A. tumefaciens LBA4404 by cocultivation method and regenerated. Regenerated letture was subject to molecular analyses for integration into plant nuclear genome and expression of ${\beta}$-glucumnidase (GUS) gene. Southern and Northern blot analyses demonstrated that GUS gene was integrated into plant nuclear genome and expressed into its mRNA. The expression of GUS gene into its protein was confirmed by specetrophotometric assay of GUS activity.

• KSBB Journal
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• v.7 no.3
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• pp.155-160
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• 1992

To improve the nutritional quality of plant proteins, a synthetic gene, called HEAAE (high essential amino acid encoding)-DNA, was introduced and expressed in tobacco plants. The synthetic gene, which is 292 basepair-long, codes for a protein composed of about 80% essential amino acids. To improve its expression level in plants, Cauliflower Mosaic Virus (CaMV) 355 and CaMV duplicate 35S promoters which are known as strong promoters were used with Nopaline Synthase promoter as a control. Transformed and regenerated tobacco plants were subject to analysis for introduction and expression of this gene. Integration of the gene into the plant genome and its expression into mRNAs and its proteins have been demonstrated using Southern, northern blot analysis and amino acid analysis. The differences of expression levels among CaMV duplicate 35S, CaMV 35S and Nopaline Synthase promoters are significant in term of mRNAs, but not in terms of proteins.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.3
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• pp.36-41
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• 2020

Mechanical systems installed in transport devices, such as vehicles, airplanes, and ships, are mostly subject to translational accelerations at the joints during operations. This base acceleration excitation has a large influence on the performance of the system, therefore, its response must be well analyzed. However, the existing methods for dynamic analysis of structures have some limitations in use. This study presents a new numerical method using relative acceleration to solve these limitations. If the governing equation of motion is linear and the mass matrix, the damping matrix, and the stiffness matrix are constant over time in the finite element analysis, the proposed method can be applied to the transient behavior analysis and the harmonic response analysis of the structure. Because it is not necessary to introduce a virtual mass and the rigid body motions are removed from the analysis, it is possible to use not only the direct integration method in the time domain but also the mode superposition method to obtain the dynamic responses. This paper demonstrates with three examples how the present method is suitable for the dynamic analysis of a structure with multi-degree of freedom.

• Journal of Information Processing Systems
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• v.16 no.2
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• pp.329-342
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• 2020

In recent years, emotional text classification is one of the essential research contents in the field of natural language processing. It has been widely used in the sentiment analysis of commodities like hotels, and other commentary corpus. This paper proposes an improved W-LDA (weighted latent Dirichlet allocation) topic model to improve the shortcomings of traditional LDA topic models. In the process of the topic of word sampling and its word distribution expectation calculation of the Gibbs of the W-LDA topic model. An average weighted value is adopted to avoid topic-related words from being submerged by high-frequency words, to improve the distinction of the topic. It further integrates the highest classification of the algorithm of support vector machine based on the extracted high-quality document-topic distribution and topic-word vectors. Finally, an efficient integration method is constructed for the analysis and extraction of emotional words, topic distribution calculations, and sentiment classification. Through tests on real teaching evaluation data and test set of public comment set, the results show that the method proposed in the paper has distinct advantages compared with other two typical algorithms in terms of subject differentiation, classification precision, and F1-measure.

• Communications of Mathematical Education
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• v.32 no.2
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• pp.131-146
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• 2018

Differentiation with integration is an important subject which is widely applied in mathematics, natural science, and engineering. Derivative is an important concept of differentiation. But students don't understand its concept well and concentrate on acquiring only the skill to solve the standardized calculus problem. So they are poor at understanding of the concept of differentiation. In this study, after making a survey of differentiation on college students, we try to analyze errors which appeared in solving differentiation problem and investigate mathematics process of limiting process inherent in the derivative and historical development about derivative. Thus, we try to analyze the understanding of differentiation and present the results about this.

• Journal of the Korea Safety Management & Science
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• v.5 no.2
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• pp.129-143
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• 2003

Bsusiness-to- Business transactions requires interactions between diverse application s and business. This gives rise to the need to for a common standard and protocol for exchanging busienss messages. and RosettaNet is the leading industry organization focused on developing B2B process standard for a global supply chain. This paper is intended as an investigation of Build-up Method of SCM Connection applying EC Standard(Rossetta-net) to reduce the integration effort, and improve extensibility and interoperability of an eBusiness infrastructure.-Intiative way for E-commerce applications to quickly incorporate new standards. Supply Chain Management (SCM) helps companies plan and execute supply to meet customer demand. The researches of modem scientists have thrown new light on the subject. but, The study of SCM for EC Standard has been strangely neglected by critics. Consequently, existing SCM system, which require to introduce the functions of EC, needs to be changed. Let me summarize the main points that have been made in this paper. The sharing information for connection and the Rosetta-net standard of the existing SCM system, and PIP 3A4, order management module, of the Rosetta-net specifications for connection by the Rosetta-net. Lastly, That shows the process of the gateway for connecting among the existing SCM systems. This provides felxibility in incorporating any new emerging open standard.

• The Journal of Society for e-Business Studies
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• v.3 no.1
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• pp.43-67
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• 1998

Logistics is by no means a new subject area. The concept of logistics goes way become more complex as technology advances, and logistics requirements have increased accordingly. In 1964, when ILS philosophy formally came into being, ILS was defined in general terms and did not describe what actions an ILS program should accomplish. ILS philosophy have been developed from 1964 through 1980. In 1982, United States Department of Defense formulated a new concept, CALS. CALS is the strategy that the US defense development to management the transition to integration and automated interchange in defense system engineering, manufacturing, and logistic support. Its goal is to use the inherent features of digitized data to revolutionize the function of data -gathering, data storage and data - transfer techologies associared with the development of defense systems. The Result will be systems that are cheaper, more reliable, and easier to maintain. To define CALS's character, the purpose of this papers compare two concepts - CALS and ILS. The elements of CALS consist of standads and EDI. The elements of ILS include LCC(Life Cycle Cost), LSA(Logistics Support Analysis), LSAR(Logistics Support Analysis Rcord), Aquisition Cycle.

• Bulletin of the Society of Naval Architects of Korea
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• v.23 no.2
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• pp.33-45
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• 1986

In the present paper, the nonlinear analysis of dynamic response of the jacket type offshore structures subject to nonlinear fluid force is performed. Furthermore, several analysis methods, such as quasi-static analysis, Newmark-$\beta$ method and state vector time integration technique, and described and compared with each others in order to investigate the efficiency numerical of the schemes for this kind of nonlinear structural analysis. In the problem formulation, various environmental forces acting on the jacket type offshore structure have been studied and calculated. Particularly, hydrodynamic forces are calculated by using the Morison type formula, which contains the interaction effect between the motion of the structure and the velocity of fluid particles. Also, Stokes' 5th order wave theory and Airy's linear wave theory are used to predict the velocity distribution of the fluid particles. Finally, the nonlinear equation of motion of the structure is obtained by using three-dimensional finite element formulation. Based on the above procedures, two examples, i.e. a single pile and a typical offshore jacket platform, are studied in details.