• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.256-260
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• 2006

This study was conducted to investigate the effect of parasititoids on the density-fluctuation of the pine needle gall midge, Thecodiplosis japonensis. The density of T. japonensis and its parasitoids was observed by emergence trap in pine forest in Namsung-dong, Keumho-eup, Youngcheon-shi, Kyeongsangbuk-do, Korea, from 1986 to 2005. After 1987, density fluctuations of adults pine needle gall midge and its parasitoids showed similar trends and density of parasitoids was dependent on density of host population. Sex ratio of the pine needle gall midge, T. japonensis, was female-biased and had some variations in according to years. These trends were similar to those of parasitoid populations and sex ratio of Inostemma seoulis was more female-biased than that of I. matsutama. T. japonensis adults emerged in the field from May 16 to July 27 and the peak of emergence was between late May and early June. The adults of Inostemma matsutama emerged from May 16 to June 11 (peak between late May and early June), whereas those of Inostemma seoulis emerged from June 9 to July 27 (peak: late June).

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.325-329
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• 2017

A micropropagation method via callus for Ranunculus kazusensis Makino, an endangered species, was established. When stem segments were cultured on MS media supplemented with 1.0 mg/L IAA, NAA, IBA and 2,4-D, the highest frequency of callus induction was achieved on MS medium supplemented with 1.0 mg/L NAA. Multiple shoot per explant was obtained, the MS medium containing 1.0 mg/L BA and 0.5 mg/L NAA. Additionally, effect of activated charcoal (AC) and sucrose on shoot growth in in vitro culture were examined. The most suitable conditions for shoot growth after 4 weeks of culture were the MS medium with AC and sucrose. This in vitro propagation protocol will be valuable for conservation and mass propagation of this endangered plant.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.726-731
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• 2013

Experiments were conducted to evaluate the effect of low temperature-darkness treatment on floral initiation in four kinds of Korean strawberry cultivars. Mother plants were planted on March 29 and daughter plants were raised for the experiment. Temperature treatment was done for 7, 14, and 21 days keeping $13^{\circ}C$ in cooling storage from August 29, September 5 and September 14 to September 21. After the treatment, flower bud initiation was examined by a microscope, and the seedlings were transplanted on hydroponic system with Yamazaki's strawberry solution of EC $0.8dS{\cdot}m^{-1}$ to check the flowering. 'Ssanta' and 'Maehyang' showed early flower bud initiation in 7 days treatment than 'Seolhyang' by microscope check. 'Ssanta', 'Daewang', and 'Maehyang' showed early flower bud initiation in 14 days treatment than 'Seolhyang'. There were no differences among the treatments in 21 days treatment. Percentage of flowering of 'Seolhyang' and 'Maehyang' by low temperature-darkness treatment didn't show any difference but 'Daewang' and 'Ssanta' showed high percentage of flowering. It suggested that low temperature-darkness treatment technique can be use for improving early flowering and yield of 'Ssanta' and 'Daewang' cultivars.

• Korean Journal of Environmental Biology
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• v.27 no.2
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• pp.155-163
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• 2009

The Oriental white stork (Ciconia boyciana) is an endangered species in Korea as well as in the world. In order to provide information on foraging habitats for releasing storks to the wild, we studied the foraging behavior of the species according to different food densities and water levels. We released two individuals, male and female, after enclosing a study site with a fence and cutting out a part of two or three primary feathers. Research was conducted from 20 June to 16 August 2008 at Whawon-ri, Mewon-myung, Chungcheongbuk-do, South Korea. We investigated habitat patch usage, foraging efficiency, and foraging methods of the feeding individuals in the different food densities and water levels. The storks invested more for foraging time and trials, and they captured more food in a habitat of high food density than others. The female invested more time and captured food most in a habitat of lowest water depth while the male in a habitat of middle water depth. However, foraging efficiency did not differ significantly among habitats of food density and water depth, and only differed between male and female by ways of foraging method. From this study we suggest that the storks select foraging habitats and foraging methods under different conditions of food density and water depth.

• Reproductive and Developmental Biology
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• v.30 no.1
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• pp.41-45
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• 2006

Electrical treatment has been widely used for porcine oocytes activation. However, developmental rates following electrical activation of porcine oocytes is relatively inefficient compared to other domestic animals. To investigate the effects of porcine oocytes on combined activation by both chemical and electrical treatment, in-vitro matured oocytes were activated by combined cycloheximide and electrical pulses treatment. Cumulus-free oocytes were exposed with NCSU-23 medium containing cycloheximide $(10{\mu}g/ml)$ for 0, 5, 10, 20, 30 min and then activated by electrical pulse treatment and cultured in PZM-3 for 8 days. Also effects of exposure to $6.25{\mu}M$ calcium ionophore for 2 min for cumulus-free oocytes were tested. The percentage of blastocyst formation in 10 min exposure to $10{\mu}g/ml$ cycloheximide and electrical pulse treatment was significantly increased (P<0.05) than in the control group. And exposure to $6.25{\mu}M$ calcium ionophore for 2 min with $10{\mu}g/ml$ cycloheximide for 10min and electrical pulse treatment significantly increased (P<0.05) the percentage of blastocyst developmental rates than the control group. In conclusion, activation by combined cycloheximide and electrical stimulation treatment promoted the subsequent development of porcine oocytes and improved the subsequence blastocyst development.

• Korean Journal of Environmental Biology
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• v.26 no.2
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• pp.121-127
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• 2008

As a step toward developing conservation and restoration plans for an endangered species of the Oriental White Stork (Ciconia boyciana), we studied daily foraging behavior and ecology. We released two individuals, male and female, after enclosing study site with a fence and cutting out a part of two or three feathers of primaries. Research was conducted from 16 June to 11 July, 2007 for 25 days at Whawonri, Mewonmyung, Chungbuk province. We investigated diural and daily variation of foraging time budget, diet composition, and habitat selection of the feeding individuals. The storks quadratically invested for foraging time and resting time according to time of day, but no significant variation in the foraging time and resting time appeared daily over 25 days. As a result, the storks mainly used wetland as a foraging site in the center of the study area to that in the edge as well as to other types of habitat. The high usage for wetland in the center did not change over the 25 days while Rice field decreased and Wetland in the edge and Pathway increased. Female invested more time for foraging than male, but the foraging efficiency did not differ between them. We discussed the significance of foraging behavior and habitat selection for management plan.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.659-662
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• 2009

The Korea Institute of Oriental White Stork Rehabilitation Research (KIOWSRR) is breeding oriental white storks for residential rehabilitation in South Korea. Three oriental white storks were referred with anorexia and depression. Two storks were died before examinations and one stork was died after 1 day of fluid therapy. On necropsy, salmon-colored subcutaneous and visceral fats were examined in all storks. Multiple focal necroses on liver surface were also examined. In gizzards, many gravels and grasses were severely tangled; therefore, it produced a large mass that blocked digestive system of the storks. Small intestinal segments of the stork, which was dead after the fluid therapy, were severely adhesive. On histopathology, diffuse necroses with/without inflammation were examined in the liver, gizzard and intestine in all storks. Bile stasis was additionally examined in the liver. According to the results of the examinations, all storks were diagnosed as gizzard impaction caused by parorexia.

• Journal of Veterinary Clinics
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• v.28 no.5
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• pp.516-518
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• 2011

A survey was conducted to examine the normal intestinal bacterial flora of captive Oriental white storks (Ciconia boyciana) maintained at the Korea Institute of Oriental White Stork Rehabilitation Research, Cheongwon, South Korea. From the cloaca of 44 healthy storks, 44 fecal samples were collected and cultured under aerobic and anaerobic conditions. The 16S ribosomal RNA gene sequences and the heat shock protein 60 gene were cloned and sequenced for bacterial identification. Under aerobic conditions, Enterococcus faecalis, Escherichia coli, Bacillus spp., Enterococcus avium, Enterococcus gallinarum, Pseudomonas spp., Alcaligenes spp., Enterobacter spp., Corynebacterium spp., and Proteus mirabilis were identified. Under anaerobic conditions, E. coli, Clostridium tertium, En. faecalis, and P. mirabilis were identified. E. coli, En. faecalis, or both were isolated from all samples. These results will add to the information available on this stork species and help for the interpretation of fecal culture results.

• (The)Study of the Eastern Classic
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• no.50
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• pp.261-294
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• 2013

The purpose of this paper lies how the original text of Chinese classical literary works have been implemented in the paintings of China and Korea, and inspect the ways how of these original text interpreted in paintings. It is an experiment of trying to analyze through literature with painting and read again painting through literature. Qu Yuan(屈原) Prose Poem of Fisherman("漁父辭"), Tao Yuanming(陶淵明) Prose Poem of Returning Home("歸去來辭") and the prose with a poem on the peach blossom spring("桃花源記幷詩"), Du Fu(杜甫), Song of Eight Drunken Celestials("飮中八仙歌"), Su Shi(蘇軾), Odes on the Red Cliff("赤壁賦"), Ou Yangxiu(歐陽脩), Odes of the Sounds of Autumn("秋聲賦") and the paintings which based on these texts were the target of examination. These literary texts shared by Chinese and Korea have been compared in the aspects of acceptance and enjoyment. And on the basis of this process the characteristics of korean paintings expressing the ideas of classical literary works was induced. As a result, the following facts are derived. First, By the emergence of the typical style which was formed historically in China at the korean painting shows that korean painters not only actively embraced the art style of China also did not lose the international sense. Second, through the profound study for chinese painting, they transformed it in accordance with korean aesthetic view and finally revealed typical korean characteristics. Third, the results as described above showed the difference of perception and interpretation of literary works between China and Korea.

• Animal Bioscience
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• v.34 no.7
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• pp.1169-1180
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• 2021

Objective: This research aimed to study the effects of Artemisia argyi flavonoids (AAF) supplemented in diets on the growth performance and immune function of broiler chickens challenged with lipopolysaccharide (LPS). Methods: A total of one hundred and ninety-two 1-d-old broiler chicks were assigned into 4 treatment groups, which were, respectively, fed a basal diet (control), fed a diet with 750 mg/kg AAF, fed a basal diet, and challenged with LPS, fed a diet with 750 mg/kg AAF, and challenged with LPS. Each treatment had six pens with 8 chicks per pen. On days 14, 16, 18, 20 (stress phase I) and 28, 30, 32, 34 (stress phase II), broilers were injected with LPS (500 ㎍/kg body weight) or an equivalent amount of saline. Results: The results demonstrated that dietary AAF significantly improved the body weight (d 21) and alleviated the decrease of average daily gain in broilers challenged with LPS on d 21 and d 35 (p<0.05). Dietary AAF increased bursa fabricius index, and dramatically attenuated the elevation of spleen index caused by LPS on d 35 (p<0.05). Furthermore, serum interleukin-6 (IL-6) concentration decreased with AAF supplementation on d 21 (p<0.05). Diet treatment and LPS challenge exhibited a significant interaction for the concentration of IL-1β (d 21) and IL-6 (d 35) in serum (p<0.05). Additionally, AAF supplementation mitigated the increase of IL-1β, IL-6 in liver and spleen induced by LPS on d 21 and 35 (p<0.05). This study also showed that AAF supplementation significantly reduced the expression of IL-1β (d 21) and nuclear transcription factor kappa-B p65 (d 21 and 35) in liver (p<0.05), and dietary AAF and LPS treatment exhibited significant interaction for the gene expression of IL-6 (d 21), toll like receptor 4 (d 35) and myeloid differentiation factor 88 (d 35) in spleen (p<0.05). Conclusion: In conclusion, AAF could be used as a potential natural immunomodulator to improve growth performance and alleviate immune stress in broilers challenged with LPS.