Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of Artemisia argyi flavonoids on growth performance and immune function in broilers challenged with lipopolysaccharide

  • Yang, Shuo (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Zhang, Jing (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Jiang, Yang (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Xu, Yuan Qing (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Jin, Xiao (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Yan, Su Mei (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Shi, Bin Lin (College of Animal Science, Inner Mongolia Agricultural University)
  • Received : 2020.09.17
  • Accepted : 2020.12.16
  • Published : 2021.07.01
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Abstract

Objective: This research aimed to study the effects of Artemisia argyi flavonoids (AAF) supplemented in diets on the growth performance and immune function of broiler chickens challenged with lipopolysaccharide (LPS). Methods: A total of one hundred and ninety-two 1-d-old broiler chicks were assigned into 4 treatment groups, which were, respectively, fed a basal diet (control), fed a diet with 750 mg/kg AAF, fed a basal diet, and challenged with LPS, fed a diet with 750 mg/kg AAF, and challenged with LPS. Each treatment had six pens with 8 chicks per pen. On days 14, 16, 18, 20 (stress phase I) and 28, 30, 32, 34 (stress phase II), broilers were injected with LPS (500 ㎍/kg body weight) or an equivalent amount of saline. Results: The results demonstrated that dietary AAF significantly improved the body weight (d 21) and alleviated the decrease of average daily gain in broilers challenged with LPS on d 21 and d 35 (p<0.05). Dietary AAF increased bursa fabricius index, and dramatically attenuated the elevation of spleen index caused by LPS on d 35 (p<0.05). Furthermore, serum interleukin-6 (IL-6) concentration decreased with AAF supplementation on d 21 (p<0.05). Diet treatment and LPS challenge exhibited a significant interaction for the concentration of IL-1β (d 21) and IL-6 (d 35) in serum (p<0.05). Additionally, AAF supplementation mitigated the increase of IL-1β, IL-6 in liver and spleen induced by LPS on d 21 and 35 (p<0.05). This study also showed that AAF supplementation significantly reduced the expression of IL-1β (d 21) and nuclear transcription factor kappa-B p65 (d 21 and 35) in liver (p<0.05), and dietary AAF and LPS treatment exhibited significant interaction for the gene expression of IL-6 (d 21), toll like receptor 4 (d 35) and myeloid differentiation factor 88 (d 35) in spleen (p<0.05). Conclusion: In conclusion, AAF could be used as a potential natural immunomodulator to improve growth performance and alleviate immune stress in broilers challenged with LPS.

Keywords

Acknowledgement

This work was supported by the National Natural Science Foundation (Project NO. 31660674). The author thanks his laboratory colleagues for their assistance in the data and sample collection and laboratory analysis.

References

  1. Li R, Song ZH, Zhao JF, et al. Dietary L-theanine alleviated lipopolysaccharide-induced immunological stress in yellow-feathered broilers. Anim Nutr 2018;4:265-72. https://doi.org/10.1016/j.aninu.2018.05.002
  2. Kamboh AA, Hang SQ, Khan MA, Zu WY. In vivo immunomodulatory effects of plant flavonoids in lipopolysaccharide-challenged broilers. Animal 2016;10:1619-25. https://doi.org/10.1017/S1751731116000562
  3. NghoNjuyi NW, Tiambo CK, Kimbi HK, Manka'a CAN, Juliano RS, Lisita F. Efficacy of ethanolic extract of carica papaya leaves as a substitute of sulphanomide for the control of coccidiosis in KABIR Chickens in cameroon. J Anim Health Prod 2015;3:21-7. https://doi.org/10.14737/journal.jahp/2015/3.1.21.27
  4. Shen YB, Piao XS, Kim SW, Wang L, Liu P. The effects of berberine on the magnitude of the acute inflammatory response induced by Escherichia coli lipopolysaccharide in broiler chickens. Poult Sci 2010;89:13-19. https://doi.org/10.3382/ps.2009-00243
  5. Kamboh AA, Zhu WY. Individual and combined effects of genistein and hesperidin on immunity and intestinal morphometry in lipopolysacharide-challenged broiler chickens. Poult Sci 2014;93:2175-83. https://doi.org/10.3382/ps.2014-03971
  6. Hager-Theodorides AL, Goliomytis M, Delis S, Deligeorgis S. Effects of dietary supplementation with quercetin on broiler immunological characteristics. Anim Feed Sci Technol 2014;198:224-30. https://doi.org/10.1016/j.anifeedsci.2014.09.021
  7. Kamboh AA, Memon AM, Mughal MJ, Memon J, Bakhetgul M. Dietary effects of soy and citrus flavonoid on antioxidation and microbial quality of meat in broilers. J Anim Physiol Anim Nutr 2018;102:235-40. https://doi.org/10.1111/jpn.12683
  8. Li YJ, Guo Y, Yang Q, et al. Flavonoids casticin and chrysosplenol D from Artemisia annua L. inhibit inflammation in vitro and in vivo. Toxicol Appl Pharmacol 2015;286:151-8. https://doi.org/10.1016/j.taap.2015.04.005
  9. Amat N, Upur H, Blazekovic B, Blaekovi B. In vivo hepatoprotective activity of theaqueous extract of Artemisia absinthium L. against chemically and immunologically induced liver injuries in mice. J Ethnopharmacol 2010;131:478-84. https://doi.org/10.1016/j.jep.2010.07.023
  10. Zhang PF, Shi BL, Yan SM, et al. Relieving effect of Artemisia argyi aqueous extract on immune stress in broilers. J Anim Physiol Anim Nutr 2017;101:251-8. https://doi.org/10.1111/jpn.12553
  11. Wang X, Huang H, Ma X, et al. Anti-inflammatory effects and mechanism of the total flavonoids from Artemisia scoparia Waldst. et kit. in vitro and in vivo. Biomed Pharmacother 2018;104:390-403. https://doi.org/10.1016/j.biopha.2018.05.054
  12. Wang DF, Zhang NY, Peng YZ, Qi DS. Interaction of zearalenone and soybean isoflavone on the development of reproductive organs, reproductive hormones and estrogen receptor expression in prepubertal gilts. Anim Reprod Sci 2010;122:317-23. https://doi.org/10.1016/j.anireprosci.2010.10.002
  13. Klaudia C, Alina W. The influence of enrofloxacin, florfenicol, ceftiofur and E. coli LPS interaction on T and B cells subset in chicks. Vet Res Commun 2015;39:53-60. https://doi.org/10.1007/s11259-015-9632-7
  14. Li Y, Zhang HZ, Chen YP, et al. Bacillus amyloliquefaciens supplementation alleviates immunological stress in lipopolysaccharide-challenged broilers at early age. Poult Sci 2015;94:1504-11. https://doi.org/10.3382/ps/pev124
  15. Zheng XC, Wu QJ, Song ZH, et al. Effects of Oridonin on growth performance and oxidative stress in broilers challenged with lipopolysaccharide. Poult Sci 2016;95:2281-9. https://doi.org/10.3382/ps/pew161
  16. Zhou Y, Mao S, Zhou M. Effect of the flavonoid baicalein as a feed additive on the growth performance, immunity, and antioxidant capacity of broiler chickens. Poult Sci 2019;98:2790-9. https://doi.org/10.3382/ps/pez071
  17. Rasouli E, Jahanian R. Improved performance and immunological responses as the result of dietary genistein supplementation of broiler chicks. Animal 2015;9:1473-80. https://doi.org/10.1017/S1751731115000853
  18. Ouyang KH, Xu M, Jiang Y, Wang WJ. Effects of alfalfa flavonoids on broiler performance, meat quality and gene expression. Can J Anim Sci 2016;96:332-41. https://doi.org/10.1139/CJAS-2015-0132
  19. Ma JS, Chang WH, Liu GH, et al. Effects of flavones of sea buckthorn fruits on growth performance, carcass quality, fat deposition and lipometabolism for broilers. Poult Sci 2015;94:2641-9. https://doi.org/10.3382/ps/pev250
  20. Chen HM, Hsu JH, Liou SF, et al. Baicalein, an active component of Scutellaria baicalensis Georgi, prevents lysophosphatidylcholine-induced cardiac injury by reducing reactive oxygen species production, calcium overload and apoptosis via MAPK pathways. BMC Complement Altern Med 2014;14:233. https://doi.org/10.1186/1472-6882-14-233
  21. Yang YF, Zhao LL, Shao YX, et al. Effects of dietary graded levels of cinnamon essential oil and its combination with bamboo leaf flavonoid on immune function, antioxidative ability and intestinal microbiota of broilers. J Integr Agric 2019;18:2123-32. https://doi.org/10.1016/S2095-3119(19)62566-9
  22. Yang L, Liu G, Zhu X, Luo Y, Shang Y, Gu XL. The anti-inflammatory and antioxidant effects of leonurine hydrochloride after lipopolysaccharide challenge in broiler chicks. Poult Sci 2019;98:1648-57. https://doi.org/10.3382/ps/pey532
  23. Wu QJ, Wang YQ, Qi YX. Influence of procyanidin supplementation on the immune responses of broilers challenged with lipopolysaccharide. Anim Sci J 2017;88:983-90. https://doi.org/10.1111/asj.12729
  24. Klasing KC, Korver DR. Leukocytic cytokines regulate growth rate and composition following activation of the immune system. J Anim Sci 1997(Suppl_2):58-67.
  25. Wu X, Cao W, Jia G, et al. New insights into the role of spermine in enhancing the antioxidant capacity of rat spleen and liver under oxidative stress. Anim Nutr 2017;3:85-90. https://doi.org/10.1016/j.aninu.2016.11.005
  26. Redmond SB, Tell RM, Coble D, et al. Differential splenic cytokine responses to dietary immune modulation by diverse chicken lines. Poult Sci 2010;89:1635-41. https://doi.org/10.3382/ps.2010-00846
  27. Liu SD, Song MH, Yun W, Lee JH, Kim HB, Cho JH. Effect of carvacrol essential oils on immune response and inflammation-related genes expression in broilers challenged by lipopolysaccharide. Poult Sci 2019;98:2026-33. https://doi.org/10.3382/ps/pey575
  28. Esmaeil N, Anaraki SB, Gharagozloo M, Moayedi B. Silymarin impacts on immune system as an immunomodulator: One key for many locks. Int Immunopharmacol 2017;50:194-201. https://doi.org/10.1016/j.intimp.2017.06.030
  29. Zhang XH, Zhong X, Zhou YM, Wang GQ, Du HM, Wang T. Dietary RRR-α-tocopherol succinate attenuates lipopolysaccharide-induced inflammatory cytokines secretion in broiler chicks. Br J Nutr 2010;104:1796-805. https://doi.org/10.1017/S0007114510002801
  30. Zeinali M, Rezaee SA, Hosseinzadeh H. An overview on immunoregulatory and anti-inflammatory properties of chrysin and flavonoids substances. Biomed Pharmacother 2017;92:998-1099. https://doi.org/10.1016/j.biopha.2017.06.003

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