• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2002.10a
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• pp.18-25
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• 2002

The pikromycin biosynthetic system in Streptomyces venezuleae is unique for its ability to produce two groups of antibiotics that include the 12-membered ring macrolides methymycin and neomethymycin, and the 14-membered ring macrolides narbomycin and pikromycin. The metabolic pathway also contains two post polyketide-modification enzymes, a glycosyltransferase and P450 hydroxylase that have unusually broad substrate specificities. In order to explore further the substrate flexibility of these enzymes a series of hybrid polyketide synthases were constructed and their metabolic products characterized. The plasmid-based replacement of the multifunctional protein subunits of the pikromycin PKS in S. venezuelae by the corresponding subunits from heterologous modular PKSs resulted in recombinant strains that produce both 12- and 14-membered ring macrolactones with predicted structural alterations. In all cases, novel macrolactones were produced and further modified by the DesVII glycosyltransferase and PikC hydroxylase leading to biologically active macrolide structures. These results demonstrate that hybrid PKSs in S. venezuelae can produce a multiplicity of new macrolactones that are modified further by the highly flexible DesVII glycosyltransferase and PikC hydroxylase tailoring enzymes. This work demonstrates the unique capacity of the S. venezuelae pikromycin pathway to expand the toolbox of combinatorial biosynthesis and to accelerate the creation of novel biologically active natural products. The polyketide backbone of rifamycin B is assembled through successive condensation and ${\beta}$-carbonyl processing of the extender units by the modular rifamycin PKS. The eighth module, in the RifD protein, contains nonfunctional DH domain and functional KR domain, which specify the reduction of the ${\beta}$-carbonyl group resulting in the C-21 bydroxyl of rifamycin B. A four amino acid substitution and one amino acid deletion were introduced in the putative NADPH binding motif in the proposed KR domain encoded by rifD. This strategy of mutation was based on the amino acid sequences of the corresponding motif of the KR domain of module 3 in the RifA protein, which is believed dysfunctional, so as to introduce a minimum alteration and retain the reading frame intact, yet ensure loss of function. The resulting strain produces linear polyketides, from tetraketide to octaketide, which are also produced by a rifD disrupted mutant as a consequence of premature termination of polyketide assembly. Much of the structural diversity within the polyketide superfamily of natural products is due to the ability of PKSs to vary the reduction level of every other alternate carbon atom in the backbone. Thus, the ability to introduce heterologous reductive segments such as ketoreductase (KR), dehydratase (DH), and enoylreductase (ER) into modules that naturally lack these activities would increase the power of the combinatorial biosynthetic toolbox. The dehydratase domain of module 7 of the rifamycin PKS, which is predicted to be nonfunctional in view of the sequence of the apparent active site, was replaced with its functional homolog from module 7 of rapamycin-producing polyketide synthase. The resulting mutant strain behaved like a rifC disrupted mutant, i.e., it accumulated the heptaketide intermediate and its precursors. This result points out a major difficulty we have encountered with all the Amycolatopsis mediterranei strain containing hybrid polyketide synthases: all the engineered strains prepared so far accumulate a plethora of products derived from the polyketide chain assembly intermediates as major products instead of just analogs of rifamycin B or its ansamycin precursors.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.10 no.4
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• pp.350-357
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• 2017

This study was conducted as a post - study on the development of a centralized controller and a hydraulic lift system including structural analysis and remote control for the development of a vertically elevated car. The safety review was carried out through the structural modification of the elevator lift which was developed during the previous research. 3D modeling was performed with Solidworks, and a model of finite element was created through Hypermesh S / W. In addition, the loading environment of the work vehicle for the evaluation is a condition in which the loading amount is 250 kg per position (total, upper, upper, lower, and lower) on the work table, ), The structural analysis was carried out under the condition that the load was 600 kg, and safety was examined in various aspects. As a result, when the allowable load of 250 kg and the excess load of 600 kg are excluded (except Case-11), the stress level is below the yield strength. In the case of Case-11, there is a region exceeding the yield strength at the center support portion of the safety bar at the upper end even after excluding the component which generates the maximum stress, but it does not affect the safety aspect of the whole structure Respectively. Looking at the deflection results, it can be seen that in all cases the maximum deflection occurs in the same table, and the tendency of sagging in both 250 kg and 600 kg is the same.

• Journal of Educational Research in Mathematics
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• v.17 no.1
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• pp.67-90
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• 2007

The purpose of this study is to understand students' thinking process in the algebra problem solving, on the base of the works of Vinner(1997a, 1997b). Thus, two middle school students were evaluated in this case study to examine how they think to solve algebra word problems. The following question was considered to analyze the thinking process from the similarity-based perspective by focusing on the process of solving algebra word problems; What is the relationship between similarity and the characteristics of thinking process at the time of successful and unsuccessful problem solving? The following results were obtained by analyzing the success or failure in problem solving based on the characteristics of thinking process and similarity composition. Successful problem solving can be based on pseudo-analytical thought and analytical thought. The former is the rule applied in the process of applying closed formulas that is constructed structural similarity not related with the situations described in the text. The latter means that control and correction occurred in all stages of problem solution. The knowledge needed for solutions was applied with the formulation of open-end formulas that is constructed structural similarity in which memory and modification with the related principles or concepts. In conclusion, the student's perception on the principles involved in a solution is very important in solving algebraic word problems.

• Journal of the Korea Concrete Institute
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• v.25 no.2
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• pp.187-194
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• 2013

Fiber reinforced polymer (FRP) has been gathering interest from designers and engineers for its possible usage as a replacement reinforcement of a steel reinforcing bar due to its advantageous characteristics such as high tensile strength, non-corrosive material, etc. Since it is manufactured with various contents ratios, fiber types, and shapes without any general specification, test results for concrete members reinforced with these FRP reinforcing bars could not be systematically used. Moreover, since investigations for FRP reinforced members have mainly focused on short-term behavior, the purpose of this study is to evaluate long-term behaviors of glass FRP (GFRP) reinforcing bar and concrete beams reinforced with GFRP. In this paper, test results of tensile and bond performance of GFRP reinforcing bar and creep behavior are presented. In the creep tests, results showed that 100 years of service time can be secured when sustained load level is below 55% of tensile strength of GFRP reinforcing bar. A modification factor of 0.73 used to calculate long-term deflection of GFRP reinforced beams was acquired from the creep tests for GFRP reinforced concrete beams. It is expected that these test results would give more useful information for design of FRP reinforced members.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.8
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• pp.279-285
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• 2019

Transverse vibrations of a ship's aft end and deckhouse are mainly induced by transverse exciting forces from the main engine. Resonance should be avoided in the initial design stages when there is a prediction of resonance between the main engine and transverse modes of the deckhouse. Estimates of frequencies for resonance avoidance are possible from the specifications of the main engine and propeller, but the inherent vibration frequency of the structure around the engine room is not easy to estimate due to the variation in the shape. Experience-oriented vibration design is also carried out, which results in many problems, such as process delay, over-injection of on-site personnel, and iterative performance of the design. For the flexible design of 8,600 TEU container vessels, this study addressed the resonance problem caused by the transverse vibration of the main engine when only the main engine was changed from 12 cylinders to 10 cylinders without modification of the hull structure layout. Efficient structural reinforcement design guidelines are presented for avoiding resonances with the main engine lateral vibration and the structure around the engine room. The guidelines are expected to be used as practical design guidelines at design sites.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.6A
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• pp.535-542
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• 2010

Precise estimation of a structure's dynamic characteristics is indispensable for ensuring stable dynamic responses during lifetime especially for the structures which can experience resonance such as railway bridges. In this paper, the results of forced vibration tests of different excitation methods (vibration exciter and impact hammer) are compared to examine the differences and the cause of differences of extracted natural frequencies. Consequently a natural frequency modification method is suggested to eliminate effects of non-structural disturbance factors. Also, sequential forced vibration tests are performed before and after track construction according to the construction stage of a railway bridge, and the variation of natural frequencies are examined. Effect of added mass of vibration exciter and variation of support condition due to the level of excitation force are concluded as the major cause of natural frequency differences. Thus eliminating these effects can enhance the reliability of the extracted natural frequencies. Construction of track affects not only the mass of structure but also the stiffness of the structure. Also, the amount of increase in stiffness varies according to the level of structural deflection. Therefore, reasonable estimation of the level of structural response during operation is important for precise natural frequency calculation at design phase.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.23 no.8
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• pp.759-769
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• 2013

Electronic equipment has been applied to virtually every area associated with commercial, industrial, and military applications. Specifically, electronics have been incorporated into avionics components installed in aircraft. This equipment is exposed to dynamic loads such as vibration, shock, and acceleration. Especially, avionics components installed in a helicopter are subjected to simultaneous sine and random base excitations. These are denoted as sine on random vibrations according to MIL-STD-810F, Method 514.5. In the past, isolators have been applied to avionics components to reduce vibration and shock. However, an isolator applied to an avionics component installed in a helicopter can amplify the vibration magnitude, and damage the chassis, circuit card assembly, and the isolator itself via resonance at low-frequency sinusoidal vibrations. The objective of this study is to investigate the dynamic characteristics of an avionics component installed in a helicopter and the structural dynamic modification of its tray plate without an isolator using both a finite element analysis and experiments. The structure is optimized by dynamic loads that are selected by comparing the vibration, shock, and acceleration loads using vibration and shock response spectra. A finite element model(FEM) was constructed using a simplified geometry and valid element types that reflect the dynamic characteristics. The FEM was verified by an experimental modal analysis. Design parameters were extracted and selected to modify the structural dynamics using topology optimization, and design of experiments(DOE). A prototype of a modified model was constructed and its feasibility was evaluated using an FEM and a performance test.

• Journal of the Korean Applied Science and Technology
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• v.34 no.2
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• pp.260-270
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• 2017

Zirconia has white color and physical, chemical stability, also using in high temperature materials and various industrial structural ceramics such as heat insulating materials and refractories due to their low thermal conductivity, excellent strength, toughness, and corrosion resistance. If hydrophobically modified zirconia is introduced into a hydrophobic acrylate coating solution, the hardness, chemical, electrical, and optical properties will be improved due to the better dispersibility of inorganic particle in organic coating media. Thus, we introduced $-CH_3$ group through silylation reaction using either trimethylchlorosilane(TMCS) or hexamethyldisilazane(HMDZ) on zirconia surface. The $Si-CH_3$ peaks derived from TMCS and HMDZ on hydrophobically modified zirconia surface was confirmed by FT-IR ATR spectroscopy, and introduction of silicon was confirmed by FE-SEM/EDS and ICP-AES. In addition, the sedimentation rate result in acrylate monomer of the modified zirconia showed the improved dispersibility. Comparison of the sizes of a pristine and the modified zirconia particles, which were clearly measured not by the normal microscope but by particle size analysis, provided a pulverizing was occurred by physical force during the silylation process. From the BET analysis data, the specific surface area of zirconia was approximately $18m^2/g$ and did not significantly change during modification process.

• Journal of Oral Medicine and Pain
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• v.24 no.4
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• pp.397-410
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• 1999

In order to obtain the basic data concerning the optimal parameters in using Nd:YAG laser as a therapeutic modality to dentinal hypersensitivity, the author prepared 3 sections of sound dentin and 10 sections of sclerotic dentin with thickness of $0.5mm{\pm}0.1mm$ from human extracted teeth of anteriors and premolars, and applied the laser energy from a fiberoptic delivered, free running, pulsed Nd:YAG laser (wavelength 1064nm, pulse duration $120{\mu}sec$, fiber diameter $320{\mu}m$) to surfaces of sound and sclerotic dentin sections for 1 second with contact/unidirectional moving mode of the fiber under speed of 3mm~4mm/sec and parameters of 0.5W/10Hz, 1.0W/10Hz, 1.5W/10Hz, 2.0W/10Hz: $62J/cm^2$, $124J/cm^2$, $187J/cm^2$, $249J/cm^2$. The author comparatively evaluated the characteristics of ultrastructural changes on surfaces of sound and sclerotic dentin sections irradiated by the pulsed Nd:YAG laser using the scanning electron microscopy. A fairly ill-defined bordered surface of partially closed and melted dentinal tubules can be seen on the scanning electron microscopic feature of the sound dentin surface irradiated by the pulsed Nd:YAG laser with energy density of $62J/cm^2$. The physical modification of sound dentin surface extensively occurred depended on the increase of energy density from $62J/cm^2$ to $124J/cm^2$, $187J/cm^2$, $249J/cm^2$. While, a fairly well-defined bordered surface of partially closed and melted dentinal tubules with thickened peritubular dentin can be seen on the scanning electron microscopic feature of the sclerotic dentin surface irradiated by the pulsed Nd:YAG laser with energy density of $62J/cm^2$. The physical modification of sclerotic dentin surface of a fairly rough, shallow depression with many cracks, thickened peritubular dentin and structureless dentinal tubules extensively occurred depended on the increase of energy density from $62J/cm^2$ to $124J/cm^2$, $187J/cm^2$, $249J/cm^2$ compared to those of sound dentin surface irradiated by the pulsed Nd:YAG laser under the same parameters. Therefore, it is recommended that the pulsed Nd:YAG laser as a therapeutic modality to dentinal hypersensitivity should be applied with the less energy density than $62J/cm^2$ on the sound dentin surface, and its energy density on the partially sclerotic dentin surface should be lower than that on the sound dentin surface to preserve tooth from unnecessary excessive structural destruction.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.34 no.4
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• pp.269-274
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• 2008

In this study we successfully altered the structural characteristics of the mica surface and were able to control oil-absorption by using the microwave enhanced etching (MEE) technique, which has originally been used in semiconductor industry. When microwave energy is applied to the mica, the surface of the mica is etched in a few minutes. As the result of etching, oil-absorption of the mica was enhanced and surface whiteness was improved by modifying the silicon dioxide layer. Additionally, the high whiteness was maintained even though the etched mica absorbed the sebum or sweat. The surface modification of mica was performed by microwave irradiation after the treatment of hydrofluoric acid. The degree of etching was regulated by acid concentration, irradiation time, the amount of energy and slurry concentration. The surface morphology of the etched mica appears to be the shape of the 'Moon'. The characteristics of surface area and roughness were examined by Brunauer-Emmett-Teller (BET) surface area analysis, atomic force microscopy (AFM), scanning electron microscopy (SEM), spectrophotometer and goniophotometer.