• Journal of the Korean Data and Information Science Society
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• 제23권3호
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• pp.559-568
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• 2012

본 연구는 보건복지가족부의 '2008년 노인실태조사'에서 얻어진 노인 자신의 삶의 질에 대한 질문을 이용하여 노인의 삶의 질이 삶의 만족도에 영향을 미치는 요인을 실증적 자료를 통해 확인하였다. 삶의 질에 대한 질문은 자아개발, 새로운 경험추구, 유행과 소비추구, 미래계획으로 이루어진 노후활동희망 요인과 동년배 비교행복감, 생활전반 만족도, 미래희망, 현재의 행복감으로 이루어진 자아통합감 요인, 가족의 노인의견존중, 연장자의 지혜, 가족관계 추구의 세대간조화의 3개의 요인으로 분류되었다. 노후활동희망이 높을수록, 자아통합감이 높을수록, 세대간조화가 높을수록 삶의 만족도는 높다고 할 수 있는데 자아통합감, 세대간조화, 노후활동희망의 순서로 삶의 만족도에 유의한 영향을 미치는 것으로 나타났다. 본 연구 결과는 노인복지서비스를 효율적으로 지원할 수 있는 방안에 도움이 되는 기본 자료로 활용할 수 있을 것이다.

• Journal of Microbiology and Biotechnology
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• 제11권5호
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• pp.763-771
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• 2001

Biphenyl dioxygenase (BPDO), which catalyzes the first step in the bacterial degradation of biphenyl and polychlorinated biphenyls, was characterized in Pseudomonas sp. B4. The bphA locus containing the four structural genes encoding BPDO were cloned and sequenced. A regulatory gene as well as a putative regulatory sequence were identified upstream of this locus. A transposase-like gene was found within a 1-kb region further upstream, thereby suggesting that the bphA locus may be carried on a transposable element. The three components of the BPDO enzyme have been separately overexpressed and purified from E. coli. The ferredoxin and terminal dioxygenase components showed biochemical properties comparable to those of two previously characterized BPDOs, whereas the ferredoxin reductase exhibited an unusually high lability. The substrate selectivity of BPDO was examined in vivo using resting cell assays performed with mixtures of selected polychlorinated biphenyls. The results indicated that para-substituted congeners were the preferred substrates. In vitro studies were carried out on a BPDO complex where the reductase from strain B4 we replaced by the more stable isoform from Comamonas testosteroni B-356. The BPDO enzyme had a specific activity of $0.26{\pm}0.02 {\mu}mol {min^-1}{mg^-1}\;of\;ISP_{BPH}$ with biphenyl as the substrate. The 2,3-, 4,4'-, and 2,4,4'-chlorobiphenyls were converted to single dihydrodiols, while 2,4'-dichlorobiphenyl gave rise to two dihydrodiols. The current data also indicated that 2,4,4'-trichlorobiphenyl was a better substrate than the 4,4'-dichlorinated congener.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.761-768
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• 2007

Serratia proteamaculans HY-3 isolated from the digestive tract of a spider produces an extracellular protease named arazyme, with an estimated molecular mass of 51.5 kDa. The purified enzyme was characterized as having high activities at wide pH and temperature ranges. We further characterized biochemical features of the enzymatic reactions under various reaction conditions. The protease efficiently hydrolyzed a broad range of protein substrates including albumin, keratin, and collagen. The dependence of enzymatic activities on the presence of metal ions such as calcium and zinc indicated that the enzyme is a metalloprotease, together with the previous observation that the proteolytic activity of the enzyme was not inhibited by aspartate, cysteine, or serine protease inhibitors, but strongly inhibited by 1,10-phenanthroline and EDTA. The araA gene encoding the exoprotease was isolated as a 5.6 kb BamHI fragment after PCR amplification using degenerate primers and subsequent Southern hybridization. The nucleotide sequence revealed that the deduced amino acid sequences shared extensive similarity with those of the serralysin family of metalloproteases from other enteric bacteria. A gene(inh) encoding a putative protease inhibitor was also identified immediately adjacent to the araA structural gene.

• 한국산학기술학회논문지
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• 제15권2호
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• pp.940-951
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• 2014

본 연구는 요양병원의 간호 인력의 간호행위 수행 실태를 파악한 조사연구이다. 연구 자료는 설문지를 이용하여 15곳의 요양병원에 근무하는 131명의 간호사를 대상으로 2013년 3월 7일부터 6월 30일까지 수집하였다. 자료 분석은 SPSS 17.0프로그램을 이용하여 기술통계 및 t-test, ANOVA를 시행하였다. '간호진단하기', '간호목표 설정하기', '환자평가표 작성'을 제외한 88항목의 간호행위를 다양한 범위 내에서 간호사와 간호조무사가 함께 수행하고 있는 것으로 나타났으며 기관의 총 환자 수와 간호사 수에 따라 간호조무사의 간호업무 수행정도는 유의한 차이가 나타났다(p<.001). 따라서 요양병원의 간호업무 중 위임할 수 있는 업무와 위임할 수 없는 업무를 분류하고, 위임에 대한 알고리즘 개발이 필요하며, 위임에 대한 법적, 제도적 규정을 마련할 필요가 있다.

• 한국콘텐츠학회논문지
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• 제17권5호
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• pp.326-336
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• 2017

본 연구는 일부 대학생의 스트레스, 자아요인 및 정서요인과 스마트폰중독정도 간의 관련성을 파악하고 스마트폰중독정도에 미치는 영향을 분석하였다. 조사대상은 J시 2개 대학에서 선정된 261명으로 하였으며, 자료수집은 2016년 12월 16일부터 12월 23일까지 자기기입식 설문지를 통해 이루어졌다. 다중회귀분석 결과, 스마트폰중독정도에 52%의 설명력으로 영향을 미치는 요인은 신체활동변화, 수면장애, 자아요인의 자기통제력, 정서요인의 충동으로 나타났다. 공분산 구조분석 결과, 스트레스, 자아요인 및 정서요인과 스마트폰중독정도의 인과관계가 확인되었다. 연구결과를 볼 때, 대학생의 스마트폰중독정도를 줄이기 위해서는 스트레스, 자아요인 및 정서요인을 관리하는 노력이 필요하다. 이러한 결과는 스마트폰중독정도를 낮추는 프로그램 및 정책 개발에 활용이 기대된다. 향후 연구에서는 스마트폰중독에 영향을 미치는 추가요인에 대한 구조모형 조사가 필요하다.

• Biomolecules & Therapeutics
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• 제27권6호
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• pp.530-539
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• 2019

Brain aging is an inevitable process characterized by structural and functional changes and is a major risk factor for neurodegenerative diseases. Most brain aging studies are focused on neurons and less on astrocytes which are the most abundant cells in the brain known to be in charge of various functions including the maintenance of brain physical formation, ion homeostasis, and secretion of various extracellular matrix proteins. Altered mitochondrial dynamics, defective mitophagy or mitochondrial damages are causative factors of mitochondrial dysfunction, which is linked to age-related disorders. Etoposide is an anti-cancer reagent which can induce DNA stress and cellular senescence of cancer cell lines. In this study, we investigated whether etoposide induces senescence and functional alterations in cultured rat astrocytes. Senescence-associated ${\beta}$-galactosidase (SA-${\beta}$-gal) activity was used as a cellular senescence marker. The results indicated that etoposide-treated astrocytes showed cellular senescence phenotypes including increased SA-${\beta}$-gal-positive cells number, increased nuclear size and increased senescence-associated secretory phenotypes (SASP) such as IL-6. We also observed a decreased expression of cell cycle markers, including PhosphoHistone H3/Histone H3 and CDK2, and dysregulation of cellular functions based on wound-healing, neuronal protection, and phagocytosis assays. Finally, mitochondrial dysfunction was noted through the determination of mitochondrial membrane potential using tetramethylrhodamine methyl ester (TMRM) and the measurement of mitochondrial oxygen consumption rate (OCR). These data suggest that etoposide can induce cellular senescence and mitochondrial dysfunction in astrocytes which may have implications in brain aging and neurodegenerative conditions.

• 한국축산식품학회지
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• 제39권5호
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• pp.844-861
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• 2019

Over the last few years, marine environment was found to be a source of surplus natural products and microorganisms with new bioactive secondary metabolites of interest which can divulge nutritional and biological impact on the host. This study aims to assess the possible, inherent and functional probiotic properties of a novel probiotic strain Enterococcus durans F3 (E. durans F3) isolated from the gut of fresh water fish Catla catla. Parameters for evaluating and describing the probiotics described in FAD/WHO guidelines were followed. E. durans F3 demonstrated affirmative results including simulated bile, acid and gastric juice tolerance with exhibited significant bactericidal effect against pathogens Staphylococcus aureus, Salmonella Typhi, Escherichia coli and Pseudomonas aeruginosa. This can be due to the enterocin produced by E. durans F3 strain, which was resolute by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gel with amplification of the anticipated fragment of a structural gene; enterocin A, followed by antibiotic susceptibility assessment. Effective antioxidant potentiality against ${\alpha}$-diphenyl-${\alpha}$-picrylhydrazyl free radicals including lipase, bile salt hydrolase activity with auto-aggregation and cell surface hydrophobicity was similarly observed. Results are proving the potentiality of E. durans F3, which can also be used as probiotic starter culture in dairy industries for manufacturing new products that imparts health benefits to the host. Finding the potent and novel probiotic strains will also satisfy the current developing market demand for probiotics.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2016년도 학술발표회
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• pp.195-195
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• 2016

Kon - Ha Thanh River basin is the largest and the most important river basin in Binh Dinh, a province in the South Central Coast of Vietnam. In the lower rivers, frequent flooding and inundation caused by heavy rains, upstream flood and or uncontrolled flood released from upstream reservoirs, are very serious, causing damage to agriculture, socio-economic activity, human livelihood, property and lives. The damage is expected to increase in the future as a result of climate change. An advanced flood warning system could provide achievable non-structural measures for reducing such damages. In this study, we applied a modelling system which intergrates a 1-D river flow model and a 2-D surface flow model for simulating hydrodynamic flows in the river system and floodplain inundation. In the model, exchange of flows between the river and surface floodplain is calculated through established links, which determine the overflow from river nodes to surface grids or vice versa. These occur due to overtopping or failure of the levee when water height surpasses levee height. A GIS based comprehensive raster database of different spatial data layers was prepared and used in the model that incorporated detailed information about urban terrain features like embankments, roads, bridges, culverts, etc. in the simulation. The model calibration and validation were made using observed data in some gauging stations and flood extents in the floodplain. This research serves as an example how advanced modelling combined with GIS data can be used to support the development of efficient strategies for flood emergency and evacuation but also for designing flood mitigation measures.

• 대한조선학회논문집
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• 제31권1호
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• pp.42-49
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• 1994

조선 CIM 시스템은 제품 모델을 중심으로 한 정보의 통합화를 중점적으로 추구하여야 하므로, 이 제품 모델을 기능 모델과 형상 모델로 구분하였다. 조선을 위한 제품 모델의 근간이 되는 형상 모델을 오일러 조작을 기초로 하는 비다양체 모델의 자료 구조를 이용하여 각각 구획 배치, 구조 설계 및 공정 계획의 입장에서 이용하는 방법에 대하여 연구하였다.

• 대한한방소아과학회지
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• 제35권3호
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• pp.118-127
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• 2021

Objective The purpose of this study was to confirm the effect of Scutellaria baicalensis extract on skin damage recovery and inflammation relief in atopic dermatitis-induced mice through Endocannabinoid system (ECS) control. Methods 6-week-old Balb/C mice were divided into control group (Ctrl), atopic dermatitis induced group (ADE), palmitoylethanolamide (PEA) administered group after atopic dermatitis induced (PEAT), and Scutellaria baicalensis extract administered group after atopic dermatitis induced (SBT). Seven animals were assigned for each group. After drug administration for 3 weeks after inducing atopic dermatitis, Claudin and 8-OHdG were observed to confirm the recovery of the skin damage in each group. To confirm ECS regulation, CB1, CB2, and GPR55 were observed. To confirm the anti-inflammatory effect, Fc ε receptor, and MMP-9 was observed. Results Claudin positive reaction was significantly increased in SBT compared to ADE and PEAT. 8-OHdG positive reaction was significantly decreased in SBT compared to ADE and PEAT. CB1, CB2, and GPR55 positive responses were significantly increased in SBT compared to ADE and PEAT. Fc ε receptor and MMP-9 positivity were significantly decreased in SBT compared to ADE and PEAT. Conclusion It was confirmed that the Scutellaria baicalensis extract can reduce the inflammation of atopic dermatitis by restoring the structural damage of the skin lipid barrier through ECS activity.