• Animal cells and systems
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• 제13권4호
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• pp.405-409
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• 2009

When DNA double-strand breaks (DSBs) were induced in mammalian cells, many DNA damage response proteins are accumulated at damage sites to form nuclear foci called IR-induced foci. Although the formation of foci has been shown to promote repair efficiency, the structural organization of chromatin in foci remains obscure. BAF53 is an actin-related protein which is required for maintenance of chromosome territory. In this study, we show that the formation of IR-induced foci by $\gamma$-H2AX and 53BP1 were reduced when BAF53 is depleted, while DSB- activated ATM pathway and the phosphorylation of H2AX remains intact after DNA damage in BAF53 knockdown cells. We also found that DSB repair efficiency was largely compromised in BAF53 knockdown cells. These results indicate that BAF53 is critical for formation of foci by $\gamma$-H2AX decorated chromatin at damage sites and the structural organization of chromatin in foci is an important factor to achieve the maximum efficiency of DNA repair.

• 한국공간구조학회논문집
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• 제23권3호
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• pp.35-43
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• 2023

This study aims to evaluate the structural safety of a structural thermal barrier, installed inside the structure of a building and performed the role of a load-bearing element and an insulation simultaneously, contributing to the realization of net-zero buildings. To ensure the reliability of the analysis model, the analysis results derived from LS-DYNA were compared with the experimental results. Based on the results shown through the flexural experiment, the reliability of the thermal cross-section insulation structure model for slabs was validated. In addition, the effect of the UHPC block on the load support performance and its contribution to vertical deflection was verified.

• 미생물학회지
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• 제49권1호
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• pp.1-7
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• 2013

유전자 재조합체는 상동염색체간의 예정된 DNA 가닥 전이와 교환이 이루어지는 상동염색체 재조합 과정에 의하여 생성된다. 이 재조합 경로는 DNA 이중 가닥 절단(double-strand breaks, DSBs)에 의해서 개시되며, 전이 과정의 중간단계에서 DNA의 구조적 변이 중간체인 단일 가닥 침투(single-end invasions, SEIs)와 이중 홀리데이 접합(double-Holliday junctions, dHJs)이 형성되어 교차성(crossover, CO) 혹은 비교차성(non-crossover, NCO) 결과물이 만들어진다. 본 연구는 이중 가닥 절단, 단일 가닥 침투, 이중 홀리데이 접합과 같은 재조합 중간체와 재조합 결과물의 구조분석에 초점을 두고, 이를 출아효모에서 인위적으로 이중 가닥 절단을 발생시킬 수 있는 HIS4LEU2 "hot spot" 을 이용한 물리적 분석방법으로 감수분열 재조합 중간체를 규명하였다. 물리적 분석을 위하여 동조화 된 세포에 감수분열을 유도한 후 hot spot 자리를 인식하는 제한효소를 처리하면, 재조합 중간체를 형성하고 있는 DNA 단편들을 Southern 분석법을 통해 탐지 및 정량 할 수 있다. 본 연구는 이 시스템으로 감수분열에서 이중가닥 절단으로부터 기인하는 단일 가닥 침투, 이중 홀리데이 접합 그리고 교차성/비교차성 재조합체로 전이되는 DNA의 구조 다형을 분석할 수 있음을 제시한다.

• JSTS:Journal of Semiconductor Technology and Science
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• 제1권1호
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• pp.15-19
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• 2001

Current leakage is the major failure mode of semiconductor device characteristic failures. Conventionally, failures such as short circuit breaks and gate breakdowns have been analyzed and the detected causes have been reflected in the fabrication process. By using a wafer-level emission-leakage failure analysis method (in-line QC), we analyzed leakage mode failure, which is the major failure detected during the probe inspection process for LSIs, typically DRAMs and CMOS logic LSIs. We have thus developed a new technique that copes with the critical structural failures and random failures that directly affect probe yields.

• 한국해양공학회지
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• 제23권3호
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• pp.59-64
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• 2009

Bridge types such as the suspension bridges and the cable stayed bridges maintained by cables present the dangerous possibility of a ship running through the bottom of the bridge. Due to hangers and main cables in the upper structural system, the bridge is also susceptible to disasters. However, these cable bridges are usually used for long span bridges over the sea. This structure is relatively more exposed to disasters, such as wind, hail, and earthquake, than other structures. This structure also has the potential to cause car accidents on account of the poor visibility due to foggy conditions. If a fire breaks out because of a car accident due to wind, a car explosion will likely occur.

• 한국항해항만학회지
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• 제44권5호
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• pp.407-413
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• 2020

In this paper we examined a novel extension of the convergence empirics for the maritime business cycle which considers structure breaks and/or changes. To provide theoretical justification, the convergence hypothesis uses the relaxed assumption to technology shocks. Based on the recent empirical results provided by Kim and Chang (2020), we consider nonlinear dynamics that capture the properties on structural changes in the equilibrium adjustment process. This approach bridges the gap between the theoretical framework and empirical specifications. In particular, we applied the convergence hypothesis to the multiple structure change model for the maritime business cycle. Our application to the maritime data showed support of the convergence hypothesis allowing multiple structure changes during the high volatile period and offers additional insight into the forecasting maritime business cycles.

• Structural Engineering and Mechanics
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• 제25권1호
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• pp.91-106
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• 2007

A new class of finite elements is described for dealing with mesh gradation. The approach employs the moving least square (MLS) scheme to devise a class of elements with an arbitrary number of nodal points on the parental domain. This approach generally leads to elements with rational shape functions, which significantly extends the function space of the conventional finite element method. With a special choice of the nodal points and the base functions, the method results in useful elements with polynomial shape functions for which the $C^1$ continuity breaks down across the boundaries between the subdomains comprising one element. Among those, (4 + n)-noded MLS based finite elements possess the generality to be connected with an arbitrary number of linear elements at a side of a given element. It enables us to connect one finite element with a few finite elements without complex remeshing. The effectiveness of the new elements is demonstrated via appropriate numerical examples.

• 응용통계연구
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• 제24권5호
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• pp.759-768
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• 2011

Tree algorithms have been widely developed for regression problems. One of the good features of a regression tree is the flexibility of fitting because it can correctly capture the nonlinearity of data well. Especially, data with sudden structural breaks such as the price of oil and exchange rates could be fitted well with a simple mixture of a few piecewise linear regression models. Now that split points are determined by chi-squared statistics related with residuals from fitting piecewise linear models and the split variable is chosen by an objective criterion, we can get a quite reasonable fitting result which goes in line with the visual interpretation of data. The piecewise linear regression by a regression tree can be used as a good fitting method, and can be applied to a dataset with much fluctuation.

• 재무관리연구
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• 제19권1호
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• pp.1-32
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• 2002

한국 종합주가지수는 한국증권거래소에 상장된 모든 기업들의 가치 가중치에 의한 포트폴리오의 가격의 시계열이라고 할 수 있다. 따라서 이 지수는 한국 경제의 현재의 활동과 미래의 활동에 대한 예상의 총합의 반영 또는 표상을 표현하는 정보라 할 수 있다. 본 논문에서는 시차변수가 독립변수로 도입될 수도 있으며, 시차변수가 아닌 변수가 독립변수로 도입되는 것이 허용되는 회귀모형을 통하여 구조변화의 회수와 구조변환점을 검정할 수 있는 통계량과 이 통계량의 확률분포를 분석하고 한국 종합주가지수에 적용하여 한국 종합주가 지수의 일별수익률에 구조변화가 발생하였는지의 여부와 발생했다면 발생회수와 변환점들을 발견하는데 그 목적이 있다. 한국 종합주가지수의 일별수익률은 분산의 변화 그리고 평균 및 분산의 동시변화가 1997년 9월 27일에 발생하였다. 자기회귀모형에 의할 때 증권시장의 구조변화는 1999년 11월 16일에 이루어졌다. 평균과 분산의 변화가 일어나 구조변화의 단계를 시작하고 구조변화에 알맞는 환경조성에 2년이 소요된 후에 1999년 11월 16일에 구조변화가 정착되었다. 정착이 이루어진 후에야 비로서 이 두 기간은 서로 다른 경제구조와 증권시장구조가 이루어지고 이에 입각하여 시장의 새로운 운동법칙이 전개되고 있다고 할 수 있을 것이다.

• 건축역사연구
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• 제21권3호
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• pp.7-28
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• 2012

This study is intended to Mindori structure which is general private houses' structural type among traditional types and is a basic study to confirm structural characteristics of Hwatong connection which is general connection type of column-beam-cross beam. It is aimed to analyze how main member, column, such as size, figure, thickness of Sungetuk and Dugeup affect on structure. Following conclusions are drawn. 1. According to connection conditions, models with big coefficient of friction show stable hysteretic behavior until the angle rotation of member reaches 1/60 and models with small coefficient of friction show dramatical increase in load after the angle rotation of member reaches 1/24. After the angle rotation of member reaches 1/30, separation distance of members is identified physically and cracks are not observed. 2. Specimens with big coefficient of friction show similar inner force regardless of column size(except column size 150mm) and models with small coefficient of friction show increasing inner force as the column size increases. Specimens with same sectional area have similar inner force even though the column figures are different. The thickness of Sungetuk and Dugeup doesn't affect inner force greatly, however, when the thickness of Sungetuk is thin, it could lead to failure of structure as it breaks. 3. The bigger the size of column and the coefficient of friction are, the smaller Bending stiffness depreciation ratio is. 4. Energy Dissipation Efficiency differs from the coefficient of friction. When the coefficient of friction is big, square column shows bigger than round one and it is bigger when the thickness of Sungetuk and Dugeup is thicker. When the coefficient of friction is small, round column shows bigger than square one.