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http://dx.doi.org/10.7845/kjm.2013.001

Identification of Meiotic Recombination Intermediates in Saccharomyces cerevisiae  

Sung, Young Jin (Department of Biomedical Sciences, CHA University)
Yoon, Sang Wook (School of Biological Sciences, Chung-Ang University)
Kim, Keun Pil (School of Biological Sciences, Chung-Ang University)
Publication Information
Korean Journal of Microbiology / v.49, no.1, 2013 , pp. 1-7 More about this Journal
Abstract
During meiosis, genetic recombinants are formed by homologous recombination accompanying with the programmed double-strand breaks (DSBs) and strand exchanges between homologous chromosomes. The mechanism is generated by recombination intermediates such as single-end invasions (SEIs) and double-Holliday junctions (dHJs), and followed by crossover (CO) or non-crossover (NCO) products. Our study was focused on the analysis of meiotic recombination intermediates (DSBs, SEIs, and dHJs) and final recombination products (CO and NCO). We identified these meiotic recombination intermediates using DNA physical analysis under HIS4LEU2 "hot spot" system in budding yeast, Saccharomyces cerevisiae. For DNA physical analysis, when the hot spot locus is recognized by restriction enzyme from synchronous meiotic cells, the fragmented DNA that are forming recombination intermediates can be detected and quantified through Southern hybridization analysis. Our study suggests that this system can analyze the structural change of recombination intermediates during DSB-SEI transition, double-Holiday junctions and crossover/non-crossover products in meiosis.
Keywords
Saccharomyces cerevisiae; crossover/non-crossover; double-strand breaks; meiotic recombination;
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