• 공업화학
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• 제25권5호
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• pp.544-547
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• 2014

본 연구에서는 효소면역 분석법(enzyme-linked immunosorbent assay)과 면역크로마토그래픽 기법을 결합하여 Legionella pneumophila 검출을 위한 면역스트립을 제작하였다. 면역스트립은 4종의 멤브레인을 이용하여 제작하였다. 니트로셀룰로오스 멤브레인은 포획항체를 고정화하여 신호 발생을 일으키기 위해 사용되었고, 두 종류의 유리섬유 멤브레인은 각각 중합체 패드와 시료주입 패드로 사용되었다. 셀룰로오스 멤브레인은 모세관 현상으로 시료흐름을 유도하는 흡수 패드로 이용하였다. 샌드위치 면역반응과 효소반응에 의해 30 min 이내에 생성된 발색신호는 정성 및 정량 분석이 가능하였다. 분석조건 하에서 육안에 의한 정성 검출뿐 아니라, $1.3{\times}10^3-1.3{\times}10^6CFU/mL$ 범위의 L. pneumophila 농도를 디지털카메라와 자체 제작된 소프트웨어를 이용하여 정량적으로 분석할 수 있었다.

• The Korean Journal of Pain
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• 제25권3호
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• pp.151-154
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• 2012

Background: The aim of this study was to document the optimal spacing of two cannulae to form continuous strip lesions and maximal surface area by using water-cooled bipolar radiofrequency technology. Methods: Two water-cooled needle probes (15 cm length, 18-gauge probe with 6 mm electrode tip) were placed in a parallel position 10, 20, 24, 26, and 28 mm apart and submerged in egg white. Temperatures of the probes were raised from $35^{\circ}C$ to $90^{\circ}C$ and the progress of lesion formation was photographed every 1 minute with the increase of the tip temperature. Approximately 30 photographs were taken. The resultant surface areas of the lesions were measured with the digital image program. Results: Continuous strip lesions were formed when the cannulae were spaced 24 mm or less apart; monopolar lesions around each cannula resulted if they were spaced more than 26 mm apart. Maximal surface areas through the formation of continuous strip lesion were 221 $mm^2$, 375 $mm^2$, and 476 $mm^2$ in 10, 20, and 24 mm, respectively. Summations of maximal surface area of each monopolar lesions were 394 $mm^2$ and 103 $mm^2$ in 26 and 28 mm, respectively. Conclusions: Water-cooled bipolar Radiofrequency technology creates continuous "strip" lesions proportional in size to the distance between the probes till the distance between cannulae is 24 mm or less. Spacing the cannulae 24 mm apart and treating about $80^{\circ}C$ for 24 minutes maximizes the surface area of the lesion.

• 공업화학
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• 제22권5호
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• pp.522-525
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• 2011

본 연구에서는, enzyme-linked immunosorbent assay (ELISA)와 면역크로마토그래픽 기법을 결합하여 Staphylococcus aureus 검출을 위한 면역스트립을 제작하였다. 면역스트립은 4개의 서로 다른 기능을 가진 멤브레인을 이용하여 만들어졌다. 니트로셀룰로오스 멤브레인은 항체와의 결합력이 높기 때문에, 포획항체를 고정화하였고, 다공성 멤브레인들을 통하여 모세관 현상으로 인해 시료흐름을 유도하였다. 효소반응에 의해 생성된 발색신호는 디지털카메라와 자체 제작된 소프트웨어를 이용하여 정성, 정량분석 하였다. 최적의 분석조건 하에서 30 min 이내에 $2.7{\times}10^4{\sim}2.7{\times}10^7CFU/mL$ 범위의 S. aureus 농도를 측정할 수 있었다.

• Journal of Multimedia Information System
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• 제8권2호
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• pp.93-100
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• 2021

Under the time-varying temperature, the high-temperature radiation of forgings and the change of reflection characteristics of oxide skin on the surface of forgings lead to the difficulty of obtaining images to truly reflect the geometric characteristics of forgings. It is urgent to study the clear and reliable acquisition method of hot forging feature image under time-varying temperature to meet the requirements of visual measurement of hot geometric parameters of forgings. Based on this, this chapter first puts forward the quality evaluation method of forging feature image, which provides guarantee for the accurate evaluation of feature image quality. Furthermore, the factors that affect the image quality, such as the radiation characteristics of forgings and the photographic characteristics of cameras, are analyzed, and the imaging spectrum which can effectively suppress the radiation intensity of forgings is determined. Finally, aiming at the problem that the quality of image acquisition is difficult to guarantee due to the drastic change of radiation intensity of forgings under time-varying temperature, an image acquisition method based on minimum signal-to-noise ratio (SNR) based laser light intensity adaptation is proposed, which significantly improves the definition of feature light strips in forging images at high temperature, and finally realizes the clear acquisition of feature images of large-scale hot forging under time-varying temperature.

• 대한기계학회논문집B
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• 제24권1호
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• pp.21-28
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• 2000

Although commercial PIV systems have been widely used for the non-intrusive velocity field measurement of fluid flows, they are still under development and have considerable room for improvement. In this study, a single-frame double-exposure PIV system using a high-resolution CCD camera was developed. A pulsed Nd:Yag laser and high-resolution CCD camera were synchronized by a home-made control circuit. In order to resolve the directional ambiguity problem encountered in the single-frame PIV technique, the second particle image was genuinely shifted in the CCD sensor array during the time interval dt. The velocity vector field was determined by calculating the displacement vector at each interrogation window using cross-correlation with 50% overlapping. In order to check the effect of spatial resolution of CCD camera on the accuracy of PIV velocity field measurement, the developed PIV system with three different resolution modes of the CCD camera (512 ${\times}$ 512, lK ${\times}$ IK, 2K ${\times}$ 2K) was applied to a turbulent flow which simulate the Zn plating process of a steel strip. The experimental model consists of a snout and a moving belt. Aluminum flakes about $1{\mu}m$ diameter were used as scattering particles for the liquid flow in the zinc pot and the gas flow above the zinc surface was seeded with atomized olive oil with an average diameter of 1-$3{\mu}m$. Velocity field measurements were carried out at the strip speed $V_s$=1.0 m/s. The 2K ${\times}$ 2K high-resolution PIV technique was significantly superior compared to the smaller pixel resolution PIV system. For the cases of 512 ${\times}$ 512 and 1K ${\times}$ 1K pixel resolution PIV system, it was difficult to get accurate flow structure of viscous flow near the wall and small vortex structure in the region of large velocity gradient.

• 한국인터넷방송통신학회논문지
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• 제21권6호
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• pp.77-82
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• 2021

요검사는 물리적 성상 검사, 화학적 검사, 현미경 검사 세 가지가 있다. 이 중에서 화학적 요검사는 일반인이 쉽게 접근하는 방법으로 요검사지의 화학반응을 눈으로 표준비색표와 비교하거나 휴대용 요검사기를 별도로 구매하여 검사를 진행한다. 현재는 스마트폰의 보급이 대중화되어 스마트폰을 활용한 요검사 서비스 연구가 높아지고 있다. 요검사 스크리닝 애플리케이션은 스마트폰을 활용한 요검사 서비스 중 하나이다. 그러나 요검사 스크리닝 애플리케이션으로 촬영한 요검사 패드 RGB 값은 조명영향으로 인해 큰 편차가 발생한다. 요검사 패드 RGB 값의 편차는 요검사 판별의 정확도를 떨어뜨린다. 따라서 본 논문에서는 스마트폰 기반 요검사 스크리닝 애플리케이션으로 촬영한 요검사지를 검사 항목별 요검사 패드로 분류한 후 CNN을 통해 요검사 패드 이미지 판별의 정확도를 높인다. 요검사지는 다양한 배경에서 촬영하여 CNN 이미지를 생성하였으며 ResNet-50 CNN 모델을 사용하여 요검사 판별을 분석하였다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1995년도 추계학술대회 논문집
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• pp.1105-1109
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• 1995

A CCD-camera, which is structured with vision system, was used to realize automatic seam-tracking system and 3-D information which is needed to generate torch path, was obtained by using laser-slip beam. To extract laser strip and obtain welding-specific point, Adaptive Hough-transformation was used. Although the basic Hough transformation takes too much time to process image on line, it has a tendency to be robust to the noises as like spatter. For that reson, it was complemented with Adaptive Hough transformation to have an on-line processing ability for scanning a welding-specific point. the dead zone,where the sensing of weld line is impossible, is eliminated by rotating the camera with its rotating axis centered at welding torch. The camera angle is controlled so as to get the minimum image data for the sensing of weld line, hence the image processing time is reduced. The fuzzy controller is adapted to control the camera angle.

• Journal of Computational Design and Engineering
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• 제2권2호
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• pp.113-118
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• 2015

An important problem in low-dose CT is the image quality degradation caused by photon starvation. There are a lot of algorithms in sinogram domain or image domain to solve this problem. In view of strong self-similarity contained in the special sinusoid-like strip data in the sinogram space, we propose a novel non-local filtering, whose average weights are related to both the image FBP (filtered backprojection) reconstructed from restored sinogram data and the image directly FBP reconstructed from noisy sinogram data. In the process of sinogram restoration, we apply a non-local method with smoothness parameters adjusted adaptively to the variance of noisy sinogram data, which makes the method much effective for noise reduction in sinogram domain. Simulation experiments show that our proposed method by filtering in both image and projection domains has a better performance in noise reduction and details preservation in reconstructed images.

• 전자공학회논문지S
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• 제35S권8호
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• pp.1-11
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• 1998

차세대 정보 통신 서비스의 고도화를 위해 추구되는 핵심 기술 중의 하나가 가시화를 통한 실감(Sensation of Reality) 서비스의 구현이다. 정보 통신 서비스의 가시화를 통한 실감화는 3차원 동영상 통신 기술의 개발없이는 구현이 불가능하다. 3차원 동영상 통신의 구현에 있어 가장 큰 문제점은 3차원 동영상에 포함된 많은 정보량을 전송할 수 있는 전송 기술과 3차원 영상을 촬영하고 실시간으로 표시할 수 있는 기술이 아직 확립되어 있지 않다는 것이다. 현재 확립되어 있는 3차원 동영상 기술은 주로 입체 방식(Stereoscopic Type)으로 실감을 얻기가 어렵다. 입체영상 보다 실감을 더해 주는 영상은 눈의 움직임과 함께 입체 영상이 연속적으로 변하게 하는 다시점(Multiview) 3차원 영상이다. 다시점 3차원 영상시스템을 8대의 카메라와 빔 프로젝터 그리고 홀로그래픽 스크린을 이용하는 시분할(Time Multiplexing) 방식에 의해 구현했다. 이 시스템에서 다시점 영상은 8대의 카메라에 의해 촬영되며, 이 촬영된 영상은 신호변환기에 의해 색상별로 한 개의 채널로 합성되어 초당 480 프레임 주파수로 빔 프로젝터에 의해 홀로그래픽 스크린에 투사된다. 빔 프로젝터의 영상은 띠형(Strip Type) 액정 셔터를 통해 홀로그래픽 스크린에 투사되게 되며, 이 띠형 액정 셔터는 홀로그래픽 스크린상에 투사된 영상을 볼 수 있게 시역을 형성한다. 각 카메라는 대응하는 띠형 액정 셔터들과 동기되어 움직이므로, 각 카메라의 영상은 대응하는 액정 셔터를 통해 투사하게 되어 시역에서는 다시점 3차원 영상의 시청이 가능해진다.

• 한국치위생학회지
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• 제13권5호
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• pp.901-908
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• 2013

Objectives : The purpose of this study was to evaluate the bleaching effect of 3% hydrogen peroxide containing strip with primer in vivo. Methods : 22 female subjects aged from 20 to 39 years old were recruited after informed consent. They were divided into two groups with control and experimental group. Experimental group was bleached with 3% HP strip with primer, and control group received placebo for 14 days. Color changes were measured with Shade Eye-NCC (Shade Eye-NCC, SHOFU Co., Japan), Shade guide (VITA classical shade guide, $VIDENT^{TM}$, CA, USA) and digital camera image. All collected data were analyzed using SPSS 18.0. Results : At baseline, $L^*$ was $71.75{\pm}3.2$ and $b^*$ was $14.90{\pm}3.6$ in control, and $L^*$ was $71.3{\pm}3.4$ and $b^*$ was $15.61{\pm}3.7$ in experimental group. At day 14, $L^*$ was $71.35{\pm}3.2$ and $b^*$ was $13.97{\pm}4.3$ in control, and $L^*$ was $73.51{\pm}2.0$, $b^*$ was $9.10{\pm}3.0$ in experimental group. ${\Delta}E^*$ of all teeth were statistically significant between the two groups. Final ${\Delta}E^*$ was $7.15{\pm}2.09$ at experimental group and $2.78{\pm}2.93$ at control group, and the difference was significant (p<0.005). Color changes using shade guide also significantly decreased at 14th day compared with baseline at experimental group (p<0.005). Conclusions : 3% hydrogen peroxide strip with primer revealed effective enough to bleaching the human enamel during 14 days without any soft and hard tissue irritations.