• Journal of Oral Medicine and Pain
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• 제26권3호
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• pp.173-180
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• 2001

스트레스가 질병 및 동통과 밀접하게 관련되어 있다는 것은 주지의 사실이며, 정서적으로 중요한 구강안면영역에는 측두하악관절장애증, 구강작열감증후군 등의 다양한 스트레스성 질환이 존재하는데, 이들의 병리학적 기전에 대해서는 아직도 논란의 여지가 있다. 그리고 중추신경계인 뇌는 스트레스 반응 및 동통 신호의 전달과 조절 등에서 중요한 역할을 하는 부분이다. 이에 저자는 스트레스와 신경과의 병리적관계를 조직학적으로 밝히고자 구속스트레스하의 백서 뇌조직을 채취하여 전자현미경으로 세포변화를 관찰하였다. 생후 8주된 Sprague-Dawley계 웅성 백서 (322-367 g/bw)를 대조군으로 3마리, 실험군으로 15마리를 배정하였다. 실험군은 구속스트레스를 실험 전기간에 걸쳐 부여하였다. 모든 실험동물의 뇌간을 적출하여, 전자현미경으로 조직변화를 관찰하였으며, 그 결과는 다음과 같다. 1. 정상 대조군에서는 정상적인 형태의 수상돌기 및 세포체가 관찰되었다. 2. 구속스트레스군의 5일군과 7일군에서 작은 크기의 사립체가 다수 출현하였다. 3. 구속스트레스 3일군부터 핵주위의 공포화(vacuolization)로 핵과 세포질이 이개되었으며, 7일군에서는 이러한 핵주위의 변성이 현저하였다. 구속스트레스 부여 후 뇌세포의 미세구조를 관찰한 결과, 세포내 미세구조 및 세포간극의 변화가 있었던 것은 스트레스에 의해 신경세포가 변성될 수 있다는 것을 의미하는 것으로, 이는 스트레스와 관련된 구강안면동통 등의 질병 기전을 밝히는데 도움이 되리라 사료되며, 향후 이에 대한 추가적인 조직학적, 분자생물학적인 연구가 필요하리라 생각된다.

• Restorative Dentistry and Endodontics
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• 제42권2호
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• pp.111-117
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• 2017

Objectives: Periapical lesions, including periapical cyst (PC), periapical granuloma (PG), and periapical abscess (PA), are frequently affected by chemical/physical damage during root canal treatment or severe bacterial infection, and thus, the differential diagnosis of periapical lesions may be difficult due to the presence of severe inflammatory reaction. The aim of this study was to make differential diagnosis among PC, PG, and PA under polarizing microscope. Materials and Methods: The collagen birefringence patterns of 319 cases of PC (n = 122), PG (n = 158), and PA (n = 39) obtained using a polarizing microscope were compared. In addition, 6 cases of periodontal fibroma (PF) were used as positive controls. Results: Collagen birefringence was condensed with a thick, linear band-like pattern in PC, but was short and irregularly scattered in PG, and scarce or absent in PA. PF showed intense collagen birefringence with a short, palisading pattern but no continuous band-like pattern. The linear band-like birefringence in PC was ascribed to pre-existing expansile tensile stress of the cyst wall. Conclusions: In this study all PCs (n = 122) were distinguishable from PGs and PAs by their characteristic birefringence, despite the absence of lining epithelium (n = 20). Therefore, the authors suggest that the presence of linear band-like collagen birefringence of the cyst wall aids the diagnostic differentiation of PC from PG and PA.

• Journal of Oral Medicine and Pain
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• 제25권3호
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• pp.265-276
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• 2000

스트레스가 타액선 조직을 변형시키고 파괴시킬 수 있다는 것은 이미 보고된 바 있다. 이는 구속스트레스 시에 관찰되는 apoptosis에 의한 것인데, 이 과정에 관여하는 caspase-3는 세포의 DNA를 분절시킴으로서 apoptosis를 일으킨다고 알려진 세포내 단백효소이다. 이에 기존에 관찰되었던 구속 스트레스에 의한 apoptosis의 형태적 변화가 apoptosis를 유도하는 caspase-3와 어떠한 시기적 상관관계를 가지고 있는지를 구명하기 위하여 본 실험을 시행하였다. 웅성 백서 (Sprague-Dawley, 8주) 를 사용하여 실험 전 기간에 걸쳐 구속스트레스를 가한 후 30분, 1시간, 3시간, 6시간, 24시간, 3일, 5일, 7일에 희생시켰다. 그 후 실험동물의 악하선을 절취하여 동결절편을 제작한 후, caspase-3에 대한 형광항체로 면역형광법을 시행하여 관찰하였다. 1. 정상대조군에서는 caspase-3가 타액선 조직 전반에서 미약하게 관찰되었다. 2. 구속스트레스 부여 30분에서 caspase-3는 강반응을 보였고, 실험기간이 경과됨에따라 점차 6시간군에서 부터는 현저히 감소하였다. 3. Caspase-3는 구속 스트레스 30분에 도관과 선포세포 모두에서 발현되었으나, 선포세포에서는 조기에 급격히 소실되었고 도관세포에서는 전 실험 기간에 걸쳐 서서히 소실되었다. 이와 같은 연구 결과에서, 세포내 caspase-3는 조직의 형태적 변화가 나타나기 이전에 발현하는 것으로 보아 caspase-3는 형태적 변화를 예견할 수 있는 진단 지표로 사용될 수 있을 것으로 사료되며 이후 임상적으로 적용하기 위한 지속적인 연구가 필요할 것으로 생각된다.

• Journal of Oral Medicine and Pain
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• 제46권4호
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• pp.161-164
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• 2021

The foramen of Huschke (FH) or foramen tympanicum is a persistent bony defect connecting the external auditory canal (EAC) to the temporomandibular joint (TMJ). It arises from an incomplete ossification of the tympanic part of the temporal bone that persists after the age of 5. If a herniation exists in the TMJ, otological symptoms may occur. An 80-year-old female patient complained of noise in her left TMJ and otorrhea in her left ear. On her cone beam computed tomography images, there were only degenerative joint disease signs on her left mandibular condyle. However, her computed tomography images revealed that the soft tissue of the TMJ herniate into the EAC. Additional examination was planned for the further evaluation. But the patient no longer visited the hospital due to her systemic health status, and symptoms disappeared spontaneously without any treatments. Usually this type of herniation is very rare, but years of mechanical stress from mastication may result in weakening and widening of the foramen with age. Therefore, although FH is usually congenital, sometimes it may be acquired in the elderly. The treatment plan should be determined in consideration of the patient's symptom level and the patient's general health status. If the clinical symptoms are not severe, no treatment is required.

• Safety and Health at Work
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• 제14권4호
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• pp.467-475
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• 2023

Background: To mitigate the spread of Covid-19, nurses infected with the virus were required to isolate themselves from their families and community. Isolated patients were reported to have experienced mental distress, posttraumatic stress disorder symptoms, and suicide. Though studies have reported the psychological impact of the Covid-19 pandemic, less is known about the lived experiences of nurses who survived Covid-19 infection in sub-Saharan Africa. Methods: A descriptive phenomenological approach was used to study the lived experiences of registered nurses who survived Covid-19 disease. In-depth interviews were conducted among nurses diagnosed with Covid-19 from two hospitals in Kenya between March and May, 2021. Purposive and snowball sampling were used to recruit registered nurses. Data were analyzed using Giorgi's steps of analysis. Results: The study included ten nurses between 29 and 45 years of age. Nurses' experiences encompassed three themes: diagnosis reaction, consequences, and coping. Reactions to the diagnosis included fear, anxiety, and sadness. The consequence of the diagnosis and isolation was stigma, isolation, and loneliness. Nurses coping mechanisms included acceptance, creating routines, support, and spirituality. Conclusion: Our findings aid in understanding how nurses experienced Covid-19 infection as patients and will provide evidence-based content for supporting nurses in future pandemics. Moreover, as we acknowledge the heroic contribution of frontline healthcare workers during the Covid-19 pandemic, it is prudent to recognize the considerable occupational risk as they balance their duty to care, and the risk of infection to themselves and their families.

• Korean Journal of Veterinary Research
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• 제41권4호
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• pp.597-602
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• 2001

This study was carried out to investigate the effectiveness of active biotelemetry to monitor stress response, and the effects of acute stressor (raising a hind foot of a standing animal) on body temperature (BT) and heart rate (HR) in comparison heifer group (n=4) with cow group (n=3). Both parameters were recorded by active telemetry. Cortisol assay was used to compare the result of active biotelemetry. The stressor consisted in restraining the cows for hoof treatments. Body temperature and heart rate were measured at 0, 10, 20, 30, 40, 50, 60, 90, 120, 180 and 360 minutes during the experiments, and also cortisol assay at 0, 0.5, 1, 2, 3, 12 and 24 hours. Base line for these animals was set in there accustomed environment at -24hrs and -30 mins before the beginning of experiments. The results obtained in this study were as follows: 1. In the BT, heifer group showed no significant difference from cow group, but were observed slightly higher BT than cow group during restraint stress for 30 minutes. 2. The both groups showed directly significant increase of HR immediately after the beginning of the restraint stress (P<0.01), but in the required time for the recovery of HR after finishing the stress, heifer group was 40 minutes and cow group 60 minutes. 3. In the cortisol assay, the both groups increased from 30 to 120 minutes after the beginning of experiment (i.e, from 0 to 90 minutes after finishing the restraint stress), showing significant difference from before the beginning of the stress (P<0.01), but heifer group showed great increase of the cortisol level from 30 to 60 minutes after the beginning of the stress, while cow group was observed at 60 minutes. In conclusion, we suppose that care is required to decrease psychological stress in cattle when they suffer from a series of procedures for diagnosis and treatment, especially in heifers rather than in cows. Also BT and HR obtained by active biotelemetry are reliable indicators of stress in cattle.

• The Korean Journal of Physiology and Pharmacology
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• 제15권6호
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• pp.345-351
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• 2011

High glucose leads to physio/pathological alterations in diabetes patients. We investigated collagen production in human gingival cells that were cultured in high concentrations of glucose. Collagen synthesis and secretion were increased when the cells were exposed to high concentrations of glucose. We examined endoplasmic reticulum (ER) stress response because glucose metabolism is related to ER functional status. An ER stress response including the expression of glucose regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), inositol requiring enzyme alpha (IRE-$1{\alpha}$) and phosphoreukaryotic initiation factor alpha (p-eIF-$2{\alpha}$) was activated in the presence of high glucose. Activating transcription factor 4 (ATF-4), a downstream protein of p-eIF-$2{\alpha}$ as well as a transcription factor for collagen, was also phosphorylated and translocalized into the nucleus. The chemical chaperone 4-PBA inhibited the ER stress response and ATF-4 phosphorylation as well as nuclear translocation. Our results suggest that high concentrations of glucose-induced collagen are linked to ER stress and the associated phosphorylation and nuclear translocation of ATF-4.

• Journal of the Korean Society of School Health
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• 제25권2호
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• pp.214-221
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• 2012

Purpose: This study was to assess the association of internet addiction and depression, stress and suicidal ideation among adolescents. Methods: The authors have surveyed 2 middle schools and 2 high schools located in Seoul and analyzed 525 questionnaires. The questionnaire included the Internet Addiction Self Diagnosis Index (K-index), Child Depression Index (CDI), Stress Index and Reynold's Suicidal Ideation Index. Structural equational model analysis was done to explore the association between internet addiction and suicidal ideation. Results: The adolescent internet addiction groups were classified as the high risk group 12 (2.3%), the potential risk group 64 (12.2%) and the normal group 449 (85.5%). When classified according to sex, the high risk group is consisted of 8 (3.9%) boys, 4 (1.3%) girls and the potential risk group is consisted of 41 (64.1%) boys and 23 (35.9%) girls which leads to the conclusion that boys are relatively more addicted compared with girls. The group with more addiction showed higher depression scores, stress scores and suicidal ideation scores. The correlation analysis showed the significant relations between internet addiction and depression (r=0.263), stress (r=0.265) and suicidal ideation (r=0.284). According to the structural equation model analysis, internet addiction had direct effects on the depression and indirect effects on suicidal ideation. Stress had the significant direct effects on both internet addiction and depression. Depression had a significant direct effect on suicidal ideation. Conclusion: Internet addiction affects directly and indirectly an depression and suicidal ideation among adolescent.

• Journal of the Korean Society of Radiology
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• 제14권4호
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• pp.415-424
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• 2020

The purpose of this study was to survey and analyze the effects of job satisfaction and job stress on organizational commitment among radiological technologist working in primary, secondary and tertiary hospitals in Busan. As a result, the scores of job satisfaction and organizational effectiveness of the tertiary hospital were significantly higher (p<0.05). In the group with no turnover experience, the organizational commitment, job satisfaction, and organizational effectiveness scores were significantly higher (p<0.05, p<0.01). Job stress was not statistically significant between variables in the analysis of general characteristics. Hierarchical regression analysis showed that the radiological technologist organizational commitment had the highest impact on job satisfaction (β=0.703, p<0.001). Radiological technologist are important in the diagnosis and radiation therapy of diseases in hospital. Therefore, based on the results of this study, it will be helpful to analyze the relationship between job satisfaction, job stress and organizational commitment of radiological technologist, and to prepare a plan to increase their organizational commitment.

• Child Health Nursing Research
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• 제10권3호
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• pp.251-261
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• 2004

Purpose: To investigate the degree and sources of stress which fathers of infants in NICU experience. Method: Eighty-five fathers of NICU infants at 5 university hospitals in Seoul volunteered to complete the Parental Stressor Scale for NICU (PSS: NICU) from September 1, to October 15, 2002. Result: Total perceived stress was 3.50±0.76. The highest score were in Parental Role Alteration (3.76±0.83) and Appearance and Behavior (3.65±1.04). The total perceived stress score (PSS), correlated significantly with method (t=3.50, p=.01), and route (F=5.30, p=.00) of feeding. Light & Sound correlated significantly with birth weight (F=3.39, p=.02), medical diagnosis(F=2.30, p=.03), plan of operation(t=2.50, p=.01), operation (t=3.80, p=.02), method (F=4.90, p=.003), and route (F=4.70, p=.00) of feeding. Infant Appearance and Behavior correlated significantly with birth weight (F=5.12, p=.01), plan of operation (t=2.50, p=.01), method (F=3.50, p=.01), and route (F=7.80, p=.00) of feeding. Parental Role Alteration correlated significantly with care of incubator (t=-.68, p=.03), method of feeding (F=2.80, p=.04) and education level of father (F=3.00, p=.05). Conclusion: Father with NICU infants were concerned mostly with parental role alteration and infant appearance. Nursing intervention should include fathers of NICU infants.