• Title/Summary/Keyword: Stress Resistance

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Physiological and Biochemical Responses of Sedum kamtschaticum and Hosta longipes to Ozone Stress (기린초와 비비추의 오존에 대한 생리·생화학적 반응)

  • Cheng, Hyo Cheng;Woo, Su Young;Lee, Seong Han;Kwak, Myeong Ja;Kim, Kyeong Nam
    • Horticultural Science & Technology
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    • v.31 no.1
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    • pp.1-7
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    • 2013
  • In this study, the resistance to ozone and characteristics of ozone-induced damage were investigated on the perennial ground cover plant species. Sedum kamtschaticum and Hosta longipes were exposed to $200{\mu}g{\cdot}kg^{-1}$ ozone for 8 hours per day (from 08:00 to 16:00) in the naturally irradiated phytotron. The extent of ozone-induced damage was measured through the analysis of physiological parameters, such as water use efficiency (WUE), chlorophyll content (Chl. a, Chl. b, Chl. a + b, and Chl. a/b ratio), carotenoid contents, and the induction of reactive oxygen species (ROS). Ozone exposure significantly reduced the daytime WUE in both species. The contents of chlorophyll and carotenoid were also decreased and ROS, such as hydrogen peroxide ($H_2O_2$) and superoxide radical ($O_2{^-}$) were accumulated after ozone exposure. The above results of this study suggested that S. kamtschaticum is more resistant to atmospheric ozone than Hosta longipes. Considering its fast responses to ozone, it was also assumed that Hosta longipes can be used as an indicator plant of an increase in atmospheric ozone concentration.

The Effect of Composite Ratio and Wall Thickness on the Shear Behavior of Composite Basement Wall (합성율과 벽체두께가 합성지하벽의 전단거동에 미치는 영향)

  • Seo, Soo-Yeon;Kim, Seong-Soo;Yoon, Yong-Dae
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.14 no.1
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    • pp.93-101
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    • 2010
  • When doing underground excavation works for the purpose of constructing large underground structures for a building in the limited space in downtown area, the stability of the adjacent ground must be top priority, and to accomplish this, it is essential to review the retaining wall construction carefully. H-Pile, which has been mainly used as a stress-carrying material in temporary earth-retaining structures, is most likely to be abandoned after completion of the works for the basement exterior wall in relation to contiguous bored piles, so it will result in a waste of material. To improve this situation, Basement Composite Wall where H-Pile and basement wall are compounded, has been developed. This wall is being used most frequently in many local construction sites. In this study, five specimens are made in order to evaluate the shear resistance of the basement composite wall and tested. Test parameter is the composition ratio and wall thickness according to shear connectors. Test result shows that the shear strength is improved when the composite ratio is increased but the magnitude is not much. A formula, which considers the contribution of concrete, web of H-pile as well as flange' effect in calculation of shear strength of composite basement wall, is suggested and used to calculation of the strength of specimens. It is found that there is a good co-relation between test result and the calculated one by the formula.

Bond Behavior of Epoxy Coated Reinforcement Using Direct Pull-out Test and Beam-End Test (직접인발시험과 보-단부 시험을 이용한 에폭시 도막 철근의 부착특성)

  • Kim, Jee-Sang;Kang, Won Hyeak
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.7 no.3
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    • pp.271-278
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    • 2019
  • The corrosion of reinforcements embedded in concrete causes severe deterioration in reinforced concrete structures. As a countermeasure, epoxy coated reinforcements are used to prevent corrosion of reinforcements. When epoxy coated bars are used, the resistance of corrosion is excellent, but epoxy coating on the bars have a disadvantage of reduction in bond capacity comparing to that of normal bars. Therefore, it is necessary to confirm the bond performance of epoxy coated reinforcements through experimental and analytical methods. Bond behaviors of epoxy coated bars for various diameters of 13 and 19mm and thicknesses of cover concrete of 3 types(ratio of cover to bar diameter) are examined. As the diameters of the epoxy coated bars increase, the difference of bond strength between epoxy coated and uncoated bars also increases and damage patterns showed pull out failure. In addition, finite element analysis was performed based on the bond-slip relationship obtained by direct pullout test and compared with the flexural test results. It is considered that flexural member test is more useful than pullout test for simulating the behavior of actual structure.

A study of applying soil moisture for improving false alarm rates in monitoring landslides (산사태 모니터링 오탐지율 개선을 위한 토양수분자료 활용에 관한 연구)

  • Oh, Seungcheol;Jeong, Jaehwan;Choi, Minha;Yoon, Hongsik
    • Journal of Korea Water Resources Association
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    • v.54 no.12
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    • pp.1205-1214
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    • 2021
  • Precipitation is one of a major causes of landslides by rising of pore water pressure, which leads to fluctuations of soil strength and stress. For this reason, precipitation is the most frequently used to determine the landslide thresholds. However, using only precipitation has limitations in predicting and estimating slope stability quantitatively for reducing false alarm events. On the other hand, Soil Moisture (SM) has been used for calculating slope stability in many studies since it is directly related to pore water pressure than precipitation. Therefore, this study attempted to evaluate the appropriateness of applying soil moisture in determining the landslide threshold. First, the reactivity of soil saturation level to precipitation was identified through time-series analysis. The precipitation threshold was calculated using daily precipitation (Pdaily) and the Antecedent Precipitation Index (API), and the hydrological threshold was calculated using daily precipitation and soil saturation level. Using a contingency table, these two thresholds were assessed qualitatively. In results, compared to Pdaily only threshold, Goesan showed an improvement of 75% (Pdaily + API) and 42% (Pdaily + SM) and Changsu showed an improvement of 33% (Pdaily + API) and 44% (Pdaily + SM), respectively. Both API and SM effectively enhanced the Critical Success Index (CSI) and reduced the False Alarm Rate (FAR). In the future, studies such as calculating rainfall intensity required to cause/trigger landslides through soil saturation level or estimating rainfall resistance according to the soil saturation level are expected to contribute to improving landslide prediction accuracy.

A Study on the Noise and Vibration Damping Performance of RC Hollow Core Slab (중공형 RC 슬래브의 소음 및 진동 감쇠성능에 대한 연구)

  • Kim, Dong Baek;Kim, In Bae;Kim, Jong Hoon;Lee, Jae Won
    • Journal of the Society of Disaster Information
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    • v.15 no.2
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    • pp.292-300
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    • 2019
  • Purpose: To reduce the noise and vibration of reinforced concrete slab structures, the damping performance is to be performed experimentally after installing hollow core or filling it with liquid. Method: Using the hollow rate as an experimental variable, the damping ratio and stiffness of each test specimen at impact load are obtained to determine the difference between the damping ratio and stiffness of the numerical analysis. In addition, the damping effects are reviewed by comparing the difference in the damping ratio and stiffness of a test specimen filled with liquid 50% of the study. Results: Since the difference in resistance between a specimen with or without hollow core is 5%, it is judged that there is no structural problem, and the injection of liquid into the hollow core can increase the damping ratio, which can reduce noise or vibration. Conclusion: At less than 20% of hollow rate, there was little damping effect, and at 30%, damping effect was found. However, if liquid is injected into the hollow core of the specimen, damping rate is shown to increase, and the injection of liquid into the hollow part is believed to reduce noise or vibration.

Evaluation of Soil Disturbance Due to Bucket Installation in Sand (모래지반에서 버켓기초 설치에 의한 지반교란 평가)

  • Kim, Jae-Hyun;Lee, Seung-Tae;Kim, Dong-Soo
    • Journal of the Korean Geotechnical Society
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    • v.34 no.11
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    • pp.21-31
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    • 2018
  • Bucket foundations are widely used in offshore areas due to their various benefits such as easy and fast installations. A bucket is installed using self-weight and the hydraulic pressure difference across the lid generated by pumping out water from inside the bucket. When buckets are installed in high permeable soil such as sands, upward seepage flow occurs around the bucket tip and interior, leading to a decrease in the effective stress in the soil inside the buckets. This process reduces the penetration resistance of buckets. However, the soil inside and outside the bucket can be disturbed due to the upward seepage flow and this can change the soil properties around the bucket. Moreover, upward seepage flow can create significant soil plug heave, thereby hindering the penetration of the bucket to the target depth. Despite of these problems, soil disturbance and soil plug heave created by suction installation are not well understood. This study aims to investigate the behavior of soil during suction installation. To comprehend the phenomena of soil plug heave during installation, a series of small-scale model tests were conducted with different testing conditions. From a series of tests, the effects of tip thickness of bucket, penetration rate, and self-weight were identified. Finally, soil properties inside the bucket after installation were approximated from the measured soil plug heave.

Verification of the Numerical Analysis on Caisson Quay Wall Behavior Under Seismic Loading Using Centrifuge Test (원심모형시험을 이용한 케이슨 안벽의 지진시 거동에 대한 수치해석 검증)

  • Lee, Jin-Sun;Park, Tae-Jung;Lee, Moon-Gyo;Kim, Dong-Soo
    • Journal of the Korean Geotechnical Society
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    • v.34 no.11
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    • pp.57-70
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    • 2018
  • In this study, verification of the nonlinear effective stress analysis is performed for introducing performance based earthquake resistance design of port and harbor structures. Seismic response of gravitational caisson quay wall in numerical analysis is compared directly with dynamic centrifuge test results in prototype scale. Inside of the rigid box, model of the gravitational quay wall is placed above the saturated sand layer which can show the increase of excess pore water pressure. The model represents caisson quay wall with a height of 10 m, width of 6 m under centrifugal acceleration of 60 g. The numerical model is made in the same dimension with the prototype scale of the test in two dimensional plane strain condition. Byrne's liquefaction model is adopted together with a nonlinear constitutive model. Interface element is used for sliding and tensional separation between quay wall and the adjacent soils. Verification results show good agreement for permanent displacement of the quay wall, horizontal acceleration at quay wall and soil layer, and excess pore water pressure increment beneath the quay wall foundation.

Differential Expression of HSP90β in MDA-MB-231 and MCF-7 Cell Lines after Treatment with Doxorubicin

  • Jokar, Fereshte;Mahabadi, Javad Amini;Salimian, Morteza;Taherian, Aliakbar;Hayat, Seyyed Mohammad Gheibi;Sahebkar, Amirhossein;Atlasi, Mohammad Ali
    • Journal of Pharmacopuncture
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    • v.22 no.1
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    • pp.28-34
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    • 2019
  • Background: Breast cancer is a complex, heterogeneous disease and one of the most common malignancies in women worldwide. The efficacy of chemotherapy as an important breast cancer treatment option has been severely limited because of the inherent or acquired resistance of cancer cells. The molecular chaperone heat shock protein 90 (HSP90) upregulated in response to cellular stress is required for functions such as conformational maturation, activation and stability in more than 200 client proteins, mostly of the signaling type. In this study, the expression of HSP90 isoforms including $HSP90{\alpha}$ and $HSP90{\beta}$ in breast cancer cell lines before and after treatment with doxorubicin (DOX) was assessed. Material and Methods: The cell cytotoxicity of DOX in MDA-MB-231 and MCF-7 cell lines was determined using the MTT assay. immunofluorescence and western blotting techniques were used to determine the expression of $HSP90{\beta}$ in the cell lines before and after DOX treatment. Immunofluorescence was also conducted to ascertain the expression of $HSP90{\alpha}$. Results: The MTT assay results showed that the MDA-MB-231 cells ($IC_{50}=14.521{\mu}M$) were more sensitive than the MCF-7 cells ($IC_{50}=16.3315{\mu}M$) to DOX. The immunofluorescence results indicated that the expression of $HSP90{\alpha}$ in both cell lines decreased after exposure to DOX. The western blot and immunofluorescence analyses showed that $HSP90{\beta}$ expression decreased in the MCF-7 cells but increased in the MDA-MB-231 cells after DOX treatment. Conclusion: The obtained results suggested that $HSP90{\alpha}$ and $HSP90{\beta}$ expression levels were reduced in the MCF-7 cells after exposure to DOX. In the MDA-MB-231 cells, $HSP90{\alpha}$ expression was reduced while $HSP90{\beta}$ was found to be overexpressed following DOX treatment.

Isolation and Characterization of Indole-3-acetic acid- and 1-aminocylopropane-1-carboxylyic Acid Deaminase-producing Bacteria Related to Environmental Stress (환경스트레스와 관련된 indole-3-acetic acid 및 1-aminocylopropane-1-carboxylyic acid deaminase 활성을 갖는 박테리아의 분리와 특성 연구)

  • Kim, Hee Sook;Kim, Ji-Youn;Lee, Song Min;Park, Hye-Jung;Lee, Sang-Hyeon;Jang, Jeong Su;Lee, Mun Hyon
    • Microbiology and Biotechnology Letters
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    • v.47 no.3
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    • pp.390-400
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    • 2019
  • In this study, strains isolated from soil samples collected from Busan, Changwon, and Jeju Island were examined to verify their abilities of phosphate solubilization and nitrogen fixation, production of indole-3-acetic acid (IAA), siderophore, and 1-aminocylopropane-1-carboxylyic acid (ACC) deaminase in order to select strains that promote plant growth and play a role in biocontrol of pests or pathogens. According to the results of this study, most of the isolated strains were found to have ability of phosphate solubilization, nitrogen fixation, IAA production, siderophore production, and production of ACC deaminase. These isolated strains might help plant growth by directly improving absorption of nutrients essential for phosphate solubilization and nitrogen fixation. In addition, they can promote plant growth and control resistance to plant diseases through extracellular enzyme activity and antifungal activity. In addition, most of the selected strains were found to survive in various environmental conditions such as temperature, salinity, and pH. Therefore, Pseudomonas plecoglossicida ANG14, Pseudarthrobacter equi ANG28, Beijerinckia fluminensis ANG34, and Acinetobacter calcoaceticus ANG35 were finally selected through a comparative advantage analysis to suggest their potential as novel biological agents. Further studies are necessary in order to prove their efficacy as novel biological agents through formulation and optimization of effective microorganisms, their preservation period, and crop cultivation tests.

New Approaches to the Control of Pathogenic Oral Bacteria (바이오필름을 생성하는 병원성 구강 세균을 제어하는 새로운 접근법)

  • Cho, Soo Jeong
    • Journal of Life Science
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    • v.31 no.1
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    • pp.100-108
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    • 2021
  • In the oral cavity, there are hundreds of microbial species that exist as planktonic cells or are incorporated into biofilms. The accumulation and proliferation of pathogenic bacteria in the oral biofilm can lead to caries and periodontitis, which are typical oral diseases. The oral bacteria in the biofilm not only can resist environmental stress inside the oral cavity, but also have a 1,000 times higher resistance to antibiotics than planktonic cells by genes exchange through the interaction between cells in the oral biofilm. Therefore, if the formation of oral biofilm is suppressed or removed, oral diseases caused by bacterial infection can be more effectively prevented or treated. In particular, since oral biofilms have the characteristic of forming a biofilm by gathering several bacteria, quorum sensing, a signaling system between cells, can be a target for controlling the oral biofilm. In addition, a method of inhibiting biofilm formation by using arginine, an alkali-producing substrate of oral bacteria, is used to convert the distribution of oral microorganisms into an environment similar to that of healthy teeth or inhibit the secretion of glucosyltransferase by S. mutans to inhibit the formation of non-soluble glucans. It can be a target to control oral biofilm. This method of inhibiting or removing the oral biofilm formation rather than inducing the death of pathogenic bacteria in the oral cavity will be a new strategy that can selectively prevent or therapeutic avenues for oral diseases including dental caries.