• 대한지역사회영양학회지
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• 제12권4호
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• pp.396-404
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• 2007

Increased oxidative stress contributes to the progression of atherosclerosis. We measured serum antioxidant mineral concentrations, capacities of serum antioxidant enzymes and fasting lipid profile in 97 male patients with coronary artery disease (CAD) and 21 male controls. Nutrient intake was assessed by the semi-quantitative food frequency method. CAD patients were divided into single-vessel disease (SVD, n=66) and multi-vessel disease (MVD, n = 31) groups on the coronary angiography. The ratio of serum LDL- to HDL-cholesterol elevated with an increasing number of diseased vessels compared to the control (control < SVD < MVD, p < 0.05). Patients with SVD and MVD had higher levels of serum lipoprotein (a) than the control (p < 0.05). The mean intake of carbohydrate, protein and cholesterol was higher in MVD patients and the intakes of vitamins C and E were lower in MVD and SVD patients than in the control (p < 0.05). Serum copper (Cu) and zinc (Zn) levels were higher in MVD and SVD patients than in the control (Cu: control $75.8{\pm}5.07$, SVD $99.2{\pm}2.90$, MVD $100.1{\pm}2.32{\mu}g/dL$, p<0.01; Zn: $76.8{\pm}5.36$, $119.0{\pm}5.95$, $129.1{\pm}2.70{\mu}g/dL$, p < 0.01). And the ratio of Zn to Cu was higher in SVD and MVD patients than in the control (control $0.78{\pm}0.06$, SVD $0.88{\pm}0.05$, MVD $0.99{\pm}0.04$, P < 0.05). The activity of glutathione peroxidase (GSH-Px) was lower in MVD than in SVD and the control (control $35.13{\pm}1.34$, SVD $35.30{\pm}1.01$, MVD $31.00{\pm}1.04 U/mg$ protein, p < 0.05). The ratio of the activities of superoxide dismutase (SOD) to GSH-Px was higher in MVD than in control and SVD (p < 0.05). In groups with CAD, serum Cu and Zn concentrations and their ratio were changed compared to the control. GSH-Px activity was decreased and the ratio of SOD to GSH-Px was increased in the patients with MVD. The balances between the activities of SOD and GSH-Px should also be considered a risk factor in CAD patients.

• 한국식품과학회지
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• 제36권4호
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• pp.568-573
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• 2004

3%의 콩단백질을 첨가한 옥수수전분 용액을 건조가열 시켜 만든 시료의 호화특성, 겔의 조직감 및 노화특성에 대해 연구하였다. 건조가열로 만들어진 시료의 경우, 단순 혼합 시료보다 낮은 pasting 온도와 높은 RVA 점도들을 보였으며 유의적으로 낮은 호화엔탈피를 보였다. 높은 온도에서 건조가열한 시료(heated sample)의 경우, 낮은 온도에서 건조가열한 시료(unheated sample)보다 pasting 온도가 낮았으며 peak viscosity, breakdown viscosity가 유의적으로 높았다. 또한 pH가 증가할수록 보다 높은 점도 profile을 보였다. 가열 건조한 시료는 낮은 파괴강도 및 파괴 strain을 보였으며 pH가 높을수록 elastic modulus와 파괴강도는 감소하고 파괴 strain은 증가하였다. 콩단백질의 첨가와 더불어 건조가열은 전분의 노화방지에 도움을 주며 겔의 미세구조를 변화시켜 겔의 조직감을 변화시켰다. 전분의 호화 및 노화특성과 겔의 조직감을 콩단백질의 첨가와 더불어 건조가열과 pH 조절을 이용하여 변화시킬 수 있었으며, 이는 전분의 호화온도와 단백질의 변성온도 이하의 온도에서 건조 후 고온 건조가열을 통해 전분-단백질의 구조적 변성과 결합을 유도하여 새로운 기능성 전분을 만들 수 있는 가능성을 보여준다.

• 동의생리병리학회지
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• 제18권6호
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• pp.1652-1660
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• 2004

Apoptosis of vascular smooth muscle cells(VSMCs) is essential in atherogenesis, being a factor that modulates its early progression rather than a terminal event in the course of the disease. Various stimuli, including oxide lipoproteins, altered hemodynamic stress and free radical, can induced VSMCs apoptosis in vitro. The protective effects of Sophorae Radix (SR) on apoptotic cell death induced by H₂O₂ were investigated in VSMCs. The viability of VSMCs was markedly decreased by H₂O₂. Sophorae Radix protected the H202-induced apoptotic death of VSMCs, which was characterized as nuclear fragmentation and increase of sub-G0/G1 fraction .. Sophorae Radix decreased the activation of caspase-3 like protease induced by H₂O₂ and recovered control level from H202-induced PARP, Bak, Bcl-XL and mitochondrial membrane potential. These results suggest that Sophorae Radix protected VSMCs apoptotic death induced by H₂O₂ via inactivation of caspase-3 and modulation of mitochondrial function. Also, the expression profile of proteins by using two-dimensional (2-D) gel electrophoresis was screened. Future investigations will need to explore the use of an anti atherosclerotic therapy of Sophorae Radix, which relies on inhibition of the proapoptotic activation of the vascular smooth muscle cells.

• Reproductive and Developmental Biology
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• 제34권3호
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• pp.143-151
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• 2010

Pluripotency of human embryonic stem cell (hESC) is one of the most valuable ability of hESCs for applying cell therapy field, but also showing side effect, for example teratoma formation. When transplant multipotent stem cell, such as mesnchymal stem cell (MSC) which retains similar differentiation ability, they do not form teratoma in vivo, but there exist limitation of cellular source supply. Accordingly, differentiation of hESC into MSC will be promising cellular source with strong points of both hESC and MSC line. In this study, we described the derivation of MSC like cell population from feeder free cultured hESC (hESC-MSC) using direct differentiation system. Cells population, hESC-MSC and bone marrow derived MSC (BM-MSC) retained similar characteristics in vitro, such as morphology, MSC specific marker expression and differentiation capacity. At the point of differentiation of both cell populations, differentiation rate was slower in hESC-MSC than BM-MSC. As these reason, to verify differentially expressed molecular condition of both cell population which bring out different differentiation rate, we compare the molecular condition of hESC-MSC and BM-MSC using 2-D proteomic analysis tool. In the proteomic analysis, we identified 49 differentially expressed proteins in hESC-MSC and BM-MSC, and they involved in different biological process such as positive regulation of molecular function, biological process, cellular metabolic process, nitrogen compound metabolic process, macromolecule metabolic process, metabolic process, molecular function, and positive regulation of molecular function and regulation of ubiquitin protein ligase activity during mitotic cell cycle, cellular response to stress, and RNA localization. As the related function of differentially expressed proteins, we sought to these proteins were key regulators which contribute to their differentiation rate, developmental process and cell proliferation. Our results suggest that the expressions of these proteins between the hESC-MSC and BM-MSC, could give to us further evidence for hESC differentiation into the mesenchymal stem cell is associated with a differentiation factor. As the initial step to understand fundamental difference of hESC-MSC and BM-MSC, we sought to investigate different protein expression profile. And the grafting of hESC differentiation into MSC and their comparative proteomic analysis will be positively contribute to cell therapy without cellular source limitation, also with exact background of their molecular condition.

• 터널과지하공간
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• 제15권1호
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• pp.1-8
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• 2005

지금까지 국내에서 적용된 내공변위 계측은 수동식 시스템 혹은 계측 정밀도가 떨어지는 방법들로서 운영중인 터널에 적용하는데 한계가 있는 것으로 평가되어 최근 국내에 도입되고 있는 자동화 내공변위 계측 시스템들을 비교 분석하고 앞으로 운영중인 지하공간과 터널에의 적용성에 대해 검토하였다. 1. 광섬유 센서를 이용한 Sofo는 국내에서 터널 라이닝의 응력 측정에는 활용된 바가 있으나 아직 터널의 내공변위와 지중 변위의 측정에 적용된 실적이 없으며 앞으로 계측 자료의 신뢰도 및 시스템의 경제성 등에 대한 충분한 검토가 요구되고 있다. 2. 각도센서와 변위센서를 이용한 TPMS는 현재 국내에서 운영중인 터널인 천안-논산간 고속도로 차령터널과 지하철 터널 및 공동구 등에서 적용중이다. 3. BCS는 두 개의 각도 센서를 한 조로 이용하는 점이 TPMS와 다른 점으로서 거의 TPMS와 같은 특성을 지니고 있다. 앞으로 센서의 정밀도가 개선될 필요가 있으며 현장에서 아주 정밀한 교류 입력 전위차를 취득하는 데는 어려움도 있어 자동화 계측 가능여부가 검증되어야 할 것으로 본다. 앞으로 터널 시공 현장에 자동계측 시스템의 검증을 위하여 TPMS 및 BCS 등을 설치 및 운영하고 기술적인 문제를 보완함으로써 이를 시공중인 주계측 단면과 운영중인 터널에 적용하여야 할 것으로 본다.

• 지역사회간호학회지
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• 제10권2호
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• pp.330-346
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• 1999

This descriptive correlational study was conducted to identify the variables related to a health promoting lifestyle in high school students. 304 high school students and 189 vocational high school students living in Taegu city were selected by a convenience sampling method. The data were collected by self-reported questionnaires from August 24 to September 4, 1998. Pender and others' Health Promoting Lifestyle Profile, Shere et al's. Self-Efficacy scale, Wallston et al.'s Internal Health Locus of Control scale, Wares' Health self rating scale and Cohen & Hoberman's Social support scale were used. The data were analyzed by using descriptive statistics, Pearson correlation coefficient, t-test, MANOVA, ANOVA. Tukey verification and Stepwise multiple regression with an SAS program. The results of this study were as follows: 1. The average score of a health promoting lifestyle performance was 2.38(SD=.36) of a 4 point scale .Self-actualization (mean = 2.80. SD = .60). interpersonal support(mean = 2.73, SD=.47), health responsibility (mean=1.53, SD=.47), nutrition(mean=2.64, SD=.63), exercise(mean=2.71. SD=.72) and stress management(mean=2.37, SD=.58) were also analyzed. 2. The combination of self-efficacy, social-support, internal health locus of control. father's educational level and perceived health status explained 37.97% of the variance in health promoting lifestyles. 3. A Health promoting lifestyle showed significant positive correlations with self efficacy(r=.5173. p=.0011), perceived health status(r=.254, p=.001) and internal health locus of control(r=.321. p=.001). 4. According to sex(t=7.38. p=.006). economic state(F=6.62, p=.00l), Father's educational level(F=4.25, p=.005), mother's educational level(F=6.94. p=.000l), the grade of educational performance(F=3.74, p=.024), sleeping hours(F=3.47. p=.032) and social support(F=40.76, p=.0001). there were significant differences and modifying factors in health promoting lifestyles. On the basis of the above findings. self-efficacy in cognitive factors, and social support in modifying factors were identified as the variables which explained most of Pender's health-promotion model. Nursing strategies enhancing self-efficacy and social support which have a more significant effect on health promoting lifestyles should be developed. Further research is required to find out the factors influencing health pormoting lifestyle of high school students.

• Journal of Plant Biotechnology
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• 제35권2호
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• pp.133-140
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• 2008

DNA와 histone 단백질의 변형은 식물 발달에 상당히 중요한 역할을 하는 것으로 알려져 있다. 식물 조직 배양 및 식물 발달 단계에서 methylation의 영향을 알아보고자 벼 종자로부터 캘러스 형성 및 식물체 재분화 단계에서 demethylation 물질인 5-azacytidine을 처리하여 유전자 발현 양상을 분석하였다. 식물체로의 재분화 능력이 있는 벼 배상체 캘러스는 5-azaC가 첨가된 H6A 배지에서는 형성되지 않았으며 갈색을 띠는 캘러스가 형성되었다. 또한 정상적인 캘러스를 5-azaC가 첨가된 MSRA 재분화 배지에서 배양했을 때도 대조구와는 달리 식물체 재분화는 이루어지지 않았다. 이러한 결과는 5-azaC가 정상적인 배상체 캘러스 및 shoot 분화에 부정적인 영향을 미친다는 것을 나타냈으며 따라서 DNA methylation이 식물 조직배양에서의 정상적인 세포 dedifferentiation과 differentiation에 필수 요인이라는 것을 알 수 있었다. 벼 캘러스 형성 및 재분화 과정 동안의 methylation 영향을 알아보고자 각 단계별로 5-azaC를 처리 후 $GeneFishig^{TM}$ DEG와 DNA chip을 사용하여 유전자 발현 양상을 분석하였다. Epigenetic regulation, 전자전달, 핵산대사, 스트레스 반응에 관여하는 일부 유전자들의 발현이 증가하거나 감소하는 것을 알 수 있었다. 발현 차이가 있는 일부 유전자를 클로닝하여 확인하였고 RT-PCR 및 northern 분석으로 각 단계에서의 발현 차이를 할인하였다.

• Applied Biological Chemistry
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• 제51권3호
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• pp.200-206
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• 2008

본 연구는 쌀 배아를 활용한 만성 대사성질환 예방용 제품 및 건강기능성 식품 개발의 일환으로써 고콜레스테롤 식이를 급여하여 고지혈증을 유발시킨 흰쥐에서 15%의 배아와 25%의 흑미 미강색소 배아젤리의 첨가가 혈장과 간 조직의 지질 대사와 항산화효소의 환성에 미치는 영향을 살펴보았다. 실험 식이를 6주 간 급여한 결과, 배아와 흑미 미강색소 배아젤리 첨가는 실험동물의 식이섭취에 영향을 미치지 않았다. 배아군과 흑미 미강색소 배아젤리군은 고콜레스테롤 급여 대조군에 비해 혈장의 총 콜레스테롤과 LDL-콜레스테롤 및 간의 중성지방과 총 콜레스테롤 농도를 감소시키고, HDL-콜레스테롤 농도와 HDL-C/TC 비는 증가시켰으며, 동맥경화지수는 감소시켜 체내 지질대사의 개선 효과가 있었다. 혈장 GOT와 GPT 수치는 배아와 흑미 미강색소 배아젤리를 첨가하였을 때 감소하여 고콜레스테롤혈증 상태에서 간 기능 보호에 긍정적인 효과가 있었다. 또한 배아와 흑미 미강색소 배아젤리는 고콜레스테롤 급여로 인해 증가된 혈장과 간 내의 지질과산화를 억제시키는 효과가 있었다. 반면, 항산화 효소인 간 조직의 SOD와 CAT활성은 배아와 배아젤리 첨가에 따라 증가하였다. 이상의 결과로 볼 때 배아와 흑미 미강색소 배아젤리는 고콜레스테롤 식이 흰쥐의 간 조직에서의 항산화 활성을 강화시키고 산화적 손상을 억제시키는 자용이 있으며 현장과 간조직의 지질대사를 개선하여 심혈관계 질환을 예방 및 감소시킬 수 있을 것으로 사료된다.

• 대한간호학회지
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• 제28권4호
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• pp.832-845
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• 1998

The purpose of this study is to analyze the effects of exercise therapy on serum lipid levels and antioxidants of obese and normal college female students. The subject group composed of ten normal-weight students(below 30% body fat ratio) and ten obese students(above 30% body fat ratio). After a pilot test, the subjects were given an eight-weeks exercise program. Before and after the exercise program, the subjects were given test for serum lipid and antioxidants were analyzed. The SAS program was used in the data analysis. The statistical measurements employed here were T-test, Wilcoxon signed rank test, and Wilcoxon rank sum test. The results of this research are as follows. 1) The effects of exercise therapy on serum lipid levels : Before the exercise therapy, the levels of Total-cholesterol, Triglyceride and LDL-cholesterol of the obese group were highier than those of the normal-weight group. However, the HDL-cholesterol levels were highier in the normal-weight group than in the obese group, but these differences were not significant. With the exercise therapy, the levels of Total-cholesterol increased gradually. The HDL-cholesterol increased gradually, the LDL-cholesterol level decreased in both groups, However, the Triglyceride level decreased in the obese group and increased in normal group, but the difference was not significant. 2) The effects of the exercise therapy on serum antioxidants ; Before exercise therapy, the serum FR and GSSG levels of the obese group were significantly highier than those of the normal-weight group(p=0.00, p=0.04). The serum GSH level of the normal-weight group was highier than that of the obese group, and the serum MDA level of the obese group was highier than that of the normal-weight group. Again these differences were not significant. With exercise therapy, serum FR level was reduced and serum GSSG level significantly increased in both group(obese group p=0.01, normal-weight group p=0.01), The serum GSH level of the obese group significantly increased (p=0.01), and serum MDA level significantly increased in the obese group(p=0.01), but the difference in the normal-weight group was not significant. These results show that regular exercise therapy reduces serum FR levels and activation of antioxidant systems, and suppress oxidative stress. These effects were slightly highier in the obese group than in the normal-weight group. The regular exercise therapy decreased the serum Triglyceride levels more in the obese group than in the normal-weight group. However the improvement of the serum lipid profile may require a longer exercise period than eight weeks. The results show that the exercise therapy was overall more effective in the obese group than the normal-weight group.

• Journal of Forest and Environmental Science
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• 제30권2호
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• pp.243-252
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• 2014

Forest tree species usually takes for long periods to be harvested and cultivated but spearmints are a good model system for woody plant because of reducing and shortening cultivation time. Spearmints are good model plants (Mentha species) for research about terpenoids production and industrial essential oil manufacture. Isopentenyl pyrophosphate isomerase (Iso) and limonene synthase (Limo) are the key enzymes of terpenoid biosynthesis pathway. Transgenic and wild spearmints (Mentha spicata, MS) were cultured in vitro and assessed for the essential oil contents. The content of essential oil of transgenic spearmint also was enhanced slightly depending on the target terpenoid genes. In an attempt to increase productivity of terpenoids further, salt and fungal elicitation strategy was adopted on transgenic Mentha spicata. The salt (800 mM NaCl) as abiotic and two fungi (Botrytis cinerea and Glomerella cingulata) as biotic were used for elicitors. In the absence of salt stress four terpenoids were detected from the spearmint extracts, all of them being monoterpenes. On the other hand, the transgenic (MSIso) extracts contained eleven terpenoids (10 monoterpenes and 1 phenylpropene) while transgenic (MSLimo) extracts contained seven monoterpenes. After 3 days of fungal infection, the resistance indices further increased to 4.38, 3.89 and 2.04 for wild type, MSIso and MSLimo, respectively. The salt and fungal elicitators proved beneficial towards modifying both the terpenoids profile and improvement in the composition of essential oil. These results have important applications for the large-scale production of essential oils and forest biotechnology with respect to spearmint.