[KSCI] Korea Science Citation Index Service

Study of Sophorae Radix on $H_2O_2$ -mediated Apoptosis and Total Protein Expression Pattern in Vascular Smooth Muscle Cells

Jeon In Cheol (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Jeong Jae Eun (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Son In Hwan (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Lee Ju Seok (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Jeong Seung Won (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Jang Jae Ho (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Lee Seon U (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Lee In (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Moon Byun Soon (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)

Publication Information

Journal of Physiology & Pathology in Korean Medicine / v.18, no.6, 2004 , pp. 1652-1660 More about this Journal

Abstract

Apoptosis of vascular smooth muscle cells(VSMCs) is essential in atherogenesis, being a factor that modulates its early progression rather than a terminal event in the course of the disease. Various stimuli, including oxide lipoproteins, altered hemodynamic stress and free radical, can induced VSMCs apoptosis in vitro. The protective effects of Sophorae Radix (SR) on apoptotic cell death induced by H₂O₂ were investigated in VSMCs. The viability of VSMCs was markedly decreased by H₂O₂. Sophorae Radix protected the H202-induced apoptotic death of VSMCs, which was characterized as nuclear fragmentation and increase of sub-G0/G1 fraction .. Sophorae Radix decreased the activation of caspase-3 like protease induced by H₂O₂ and recovered control level from H202-induced PARP, Bak, Bcl-XL and mitochondrial membrane potential. These results suggest that Sophorae Radix protected VSMCs apoptotic death induced by H₂O₂ via inactivation of caspase-3 and modulation of mitochondrial function. Also, the expression profile of proteins by using two-dimensional (2-D) gel electrophoresis was screened. Future investigations will need to explore the use of an anti atherosclerotic therapy of Sophorae Radix, which relies on inhibition of the proapoptotic activation of the vascular smooth muscle cells.

Keywords

Sophorae Radix; caspase-3; PARP; Bak; Bcl-XL; total protein expression;

Citations & Related Records

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KSCI

Study of Sophorae Radix on -mediated Apoptosis and Total Protein Expression Pattern in Vascular Smooth Muscle Cells 고삼이 에 의한 대동맥 평활근세포 고사 및 전체 단백질 발현에 미치는 영향

Study of Sophorae Radix on $H_2O_2$ -mediated Apoptosis and Total Protein Expression Pattern in Vascular Smooth Muscle Cells