• Title/Summary/Keyword: Stress Concentration Ratio

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Physiological and Proteomic Responses of Barley Seedlings to Salt Stress (보리의 생육초기 염 스트레스에 따른 생리적 반응과 프로테옴 변화)

  • Kim, Dea-Wook;Yun, Seong-Kun;Park, Hyoung-Ho;Hwang, Jong-Jin;Han, Ok-Kyu;Park, Tae-Il;Jung, Gun-Ho;Lee, Jae-Eun;Kim, Sun-Lim;Chung, Young-Ho
    • Journal of the Korean Society of International Agriculture
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    • v.23 no.5
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    • pp.537-545
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    • 2011
  • This study was conducted to obtain basic information on physiological and proteomic responses of barley seedlings to salt stress. Shoot dry weight decreased significantly as the level of soil salinity increased. Salt stress-induced decrease of relative shoot dry weight was lower in cv. "Sanglok" than in cv. "Sunwoo". Under the salt stress, SPAD value decreased, and the value was higher in cv. "Sanglok" than in cv. "Sunwoo". Sodium ion content in the leaves increased as NaCl concentration increased, and the content was higher in cv. "Sunwoo" than in cv. "Sanglok". The K+/Na+ ratio was higher in cv. "Sanglok" than in cv. "Sunwoo". Salt stress-induced alterations in protein expression of the leaves were detected by two dimensional electrophoresis, and 47 protein spots showing altered expression were selected. Among the selected protein spots, 17 protein spots were up-regulated and 28 spots down-regulated in cv. "Sanglok". In cv. "Sunwoo", 14 protein spots were up-regulated and 27 spots down-regulated. Out of 47 deferentially expressed protein spots, 18 protein spots were identified using mass spectrometry and NCBI protein database. Among the identified proteins, ten proteins are known to be involved in various stress responses, but the others are not directly involved in stress responses.

Effects of Egg Gel Formation According to Mixing Ratio of Sugar Sources, NaCl and Sucrose (당 종류 및 NaCl과 Sucrose 배합비에 따른 계란찜의 겔 형성 효과)

  • Kim, Kyung-Mee;Kim, Ok-Sun
    • Journal of the East Asian Society of Dietary Life
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    • v.18 no.1
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    • pp.72-79
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    • 2008
  • This study investigated the changes in textural characteristics that occurred by adding maltose syrup, dextrin, and sucrose to whole egg gels, by assessing coagulation after cooling. It also examined the optimal NaCl and sucrose concentrations for whole egg gels sensory evaluations, and then studied how the addition of sucrose effected gel formation and textural characteristics under optimal NaCl concentration. The additions of maltose syrup, dextrin, and sucrose, presented some color changes. The greater the addition of maltose syrup or dextrin, the lower the L, a, and b values of the whole egg gel and whole egg liquid, and ultimately the color turned dark bluish green. With increasing additions of sucrose, maltose syrup, and dextrin, the viscosity of the whole egg liquid increased slightly. In terms of the mechanical texture characteristic of the gel, the texture was most elastic with the 0.8% addition of sucrose, and hardness decreased by increasing the ratio of added sucrose. Increasing amounts of maltose syrup resulted in less hardness and SF. And for dextrin, the SF increased up to 2.5 and then decreased, and hardness decreased with increasing amounts of dextrin. Based on sensory evaluations, the 0.8% addition of NaCl was significantly preferred(p<0.05), in terms of salty taste. The overall preference scores indicated that the whole egg gel made with 0.3% sucrose and the optimal NaCl concentration(0.8%) was most preferred, and each sample was significant(p<0.05). Under the optimal 0.8% NaCl concentration increasing the sucrose concentration resulted in a darker egg gel color, in terms the L value. SF, NF, and hardness, which are mechanical texture parameters, were when 0.8% sucrose and the optimal NaCl concentration of 0.8% were added to whole egg liquid, in preparing the whole egg gel.

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Effects of Rice Embryo and Embryo Jelly with Black Rice Bran Pigment on Lipid Metabolism and Antioxidant Enzyme Activity in High Cholesterol-Fed Rats (쌀 배아와 흑미 미강 색소 첨가 배아젤리가 고콜레스테를 식이 흰쥐의 지질대사와 항산화효소 활성에 미치는 영향)

  • Cho, Min-Kyung;Kim, Mi-Hyun;Kang, Mi-Young
    • Applied Biological Chemistry
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    • v.51 no.3
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    • pp.200-206
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    • 2008
  • We investigated the effect of rice embryo and embryo jelly with black rice bran pigment on lipid metabolism and antioxidant activity. Thirty 4-week-old male Sprague-Dawley rats were fed high cholesterol diets supplemented with 15% rice embryo and 25% embryo jelly added black rice bran pigment, respectively, for 6 weeks. Plasma and hepatic lipid profile, lipid peroxidation, and the activity of antioxidant scavenger enzymes in liver were examined. Supplementation with rice embryo and embryo jelly had no effect on food intakes in high cholesterol-fed rats. The plasma triglyceride concentration was not significantly different among the groups. Supplementation with rice embryo and embryo jelly resulted in lower plasma and hepatic total cholesterol (TC) concentration and high-density lipoprotein-cholesterol (HDL-C)/TC ratio and atherogenic index compared to the control group, while the plasma HDL-C concentration tended to elevated. Rice embryo and embryo jelly tended to lower plasma and hepatic levels of thiobarbituric acid reactive substances than the control group. Moreover, hepatic antioxidant enzyme activities, including superoxide dismutase and glutathione peroxidase, were significantly higher in the rice embryo and embryo jelly groups. In conclusion, rice embryo and embryo jelly was very effective in improving the lipid metabolism and reducing oxidative stress by up-regulating the hepatic antioxidant enzymes in high cholesterol-fed rats.

Investigation on Translocation of De-icing Salts influenced by the Intensity of Foliar Damage of Roadside Trees in Chung-ju City (충주시 가로수의 황변정도에 따른 토양 내 제설제 성분의 흡수이행성 평가)

  • Kim, Jae-Young;Kim, Won-Tae;Yoon, Young-Han;Ju, Jin-Hee
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.21 no.4
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    • pp.1-10
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    • 2018
  • Use of de-icing salts results in accumulation of high concentrations of ions on roadside soils and tree. The purpose of this study isto determine translocation of seasonal impact of exchangeable cations originating from de-icing salt on roadside surface soil-plant influenced by the intensity of foliar damage (NY = 0-25%, SY = 26-50%, CY = 51-75%) of trees. This paper investigated the concentration of four exchangeable cations ($K^+$, $Ca^{2+}$, $Na^+$, and $Mg^{2+}$) on the roadside surface soil. The tree (Ginko biloba) samples were collected from the Konkuk and Judeok intersections in Chung-ju city. The sequential extraction procedure was applied to 120 soil samples of the soilsurface and 30 tree samples. Four cation exchange ions were determined by ICP-OES. The content of four exchangeable cations present on roadside soil was found to be the lowest in NY but highest in CY from tree pits in the order of NY < SY < CY. Especially, the results were apparent during spring time compared to other seasons. Soil collected from tree pits had the highest concentration of $Ca^{2+}$ possibly due to a higher volume of traffic on those streetsresulting in splashing of more calcium chloride ($CaCl_2$). The analysis of three exchangeable cations ($K^+$, $Mg^{2+}$, and $Na^+$) in the tree leaves revealed higher levels than roadside surface soil when foliar damage ratio increased in the order of NY < SY < CY in summer. In addition, a strong positive linear relationship was observed between the concentration of exchangeable cations in soil and trees. It is hypothesized that the results of this study can be a valuable baseline for managing de-icing salt on roadside soil and trees, in order to mitigate the salt stress that can damage the roadside soil and trees.

Iron sulfate and molasses treated anthocyanin-rich black cane silage improves growth performance, rumen fermentation, antioxidant status, and meat tenderness in goats

  • Rayudika Aprilia Patindra, Purba;Ngo Thi Minh, Suong;Siwaporn, Paengkoum;Pramote, Paengkoum;Juan Boo, Liang
    • Animal Bioscience
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    • v.36 no.2
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    • pp.218-228
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    • 2023
  • Objective: This study investigated the effects of feeding anthocyanin-rich black cane treated with ferrous sulfate and molasses on animal performance, rumen fermentation, microbial composition, blood biochemical indices, and carcass characteristics in meat goats. Methods: Thirty-two Thai-native×Anglo-Nubian crossbred male goats (14.47±2.3 kg) were divided equally into two groups (n = 16) to investigate the effect of feeding diet containing 50% untreated anthocyanin-rich black cane silage (BS) vs diet containing anthocyaninrich black cane silage treated with 0.03% ferrous sulfate and 4% molasses (TBS) on average daily gain (ADG) and dry matter intake (DMI). At the end of 90 d feeding trial, the goats were slaughtered to determine blood biochemical indices, rumen fermentation, microbial composition, and carcass characteristics differences between the two dietary groups. Results: Goats fed the TBS diet had greater ADG and ADG to DMI ratio (p<0.05). TBS diet did not affect rumen fluid pH; however, goats in the TBS group had lower rumen ammonia N levels (p<0.05) and higher total volatile fatty acid concentrations (p<0.05). Goats in the TBS group had a higher (p<0.05) concentration of Ruminococcus albus but a lower (p<0.05) concentration of methanogenic bacteria. The TBS diet also resulted in lower (p<0.05) thiobarbituric acid-reactive substances concentration but higher (p<0.05) total antioxidant capacity, superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase concentrations in blood plasma, while having no effect on plasma protein, glucose, lipid, immunoglobin G, alanine transaminase, and aspartate aminotransferase. Meat from goats fed the TBS diet contained more intramuscular fat (p<0.05) and was more tender (p<0.05). Conclusion: In comparison to goats fed a diet containing 50% untreated anthocyanin-rich black cane silage, feeding a diet containing 50% anthocyanin-rich black cane silage treated with 0.03% ferrous sulfate and 4% molasses improved rumen fermentation and reduced oxidative stress, resulting in higher growth and more tender meat.

Association of the Nutritional Status and Essential Amino Acids Intake in Hemodialysis Patients (혈액투석환자에서 영양상태와 필수아미노산 섭취의 관련성 연구)

  • Kim, Hye-Jin;Kim, Su-An;Sohn, Cheong-Min
    • Journal of Nutrition and Health
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    • v.39 no.7
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    • pp.617-623
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    • 2006
  • The mortality and morbidity rate of hemodialysis patients (HD) remain high. Among many factors, protein and calorie malnutrition has been shown to be a major risk factor for increased mortality in the HD patients population. Malnutrition can be caused by insufficient amino acid intake, nutrient losses in dialysate, oxidant stress and muscle catabolism. In this study, we evaluated the association of markers of nutritional status and essential amino acids intake in HD patients. We investigated nutritional status of 41 HD patients (mean age: $64.2\;{\pm}\;11.5\;y$, men: 24, women: 27) by measuring anthropometric, biochemical parameters and food intakes by using 24 hr recall methods. Subject's total energy intake and total protein intake were $1,648.0\;{\pm}\;397.31\;kcal/day,\;79.2\;{\pm}\;27.2\;g/day$:, respectively. The animal protein intake was $42.7\;{\pm}\;22.1\;g/day$, essential amino acids intake was $23.4\;{\pm}\;9.92\;g/day$, and the ratio of essential amino acids to total protein intake was $29.6\;{\pm}\;5.42%$. There were significantly positive correlation between muscle mass and lean body mass with serum creatinine level (r=0.435, p<0.01; r=0.435, p<0,01). There were also significant positive correlation in muscle mass and lean body mass with pre hemodialysis blood urea nitrogen (preHD BUN) (r=0.329, p<0.05; r=0.329, p<0.05). There were no significant correlation in total energy intake and total protein intake per kg ideal body weight (IBW) to muscle mass and lean body mass. However, there were significantly positive correlation between the ratio of essential amino acids and muscle mass and lean body mass (r=0.368, p<0.05; r=0.405, p<0.01). And serum hematocrit concentration was positively correlated with the ratio of essential amino acids (r=0.032, p<0.05). The results of this study indicate that strong associations exist in essential amino acid intakes with malnutrition than total protein intakes in HD patient. In conclusion, specialized nutrition education should be necessary to efficiently improve the quality of protein intakes.

Effect of Calcium Chloride Concentration on Roadside Ground Cover Plant Growth (염화칼슘 처리농도가 가로변 지피식물의 생장에 미치는 영향)

  • Lee, Sun-Young;Kim, Won-Tae;Ju, Jin-Hee;Yoon, Yong-Han
    • Journal of the Korean Institute of Landscape Architecture
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    • v.41 no.4
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    • pp.17-23
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    • 2013
  • The purpose of this study was to provide information on management and apply it to a roadside ground cover plant understanding the capacity of calcium chloride in the plant. The experimental group was composed of the ratio control group of calcium chloride, 0.5%, 1.0%, and 3.0% in 500g of soil. Plant materials were selected and measured according to their ecological characteristics such as ground cover plant, Pachysandra terminalis, Hosta plantaginea, Trachelospermum asiaticum, Vitex rotundifolia, Euonymus japonica and Callicarpa japonica. The acidity of the amended soil was increased gradually depending on the treatment and conductivity was continually decreased. The EX-Ca increased after the treatment, but decreased in the middle of the experiment. Pachysandra terminalis, Trachelospermum asiaticum and Euonymus japonica were able to grow and survive at the ratio of 0.5%. Hosta plantaginea and Vitex rotundifolia were able to survive at the ratio of 1.0%. Hosta plantaginea, the possible state can absorb salts due to moisture and, can be applied to ground cover plants in the roadside. The growth and development of Callicarpa japonica was poor and the leaves were open to grow for calcium chloride treatment except the control group. It was concluded that Callicarpa japonica was very sensitive to calcium chloride.

A Study on Antioxidant System in Cataract Patients (한국인 백내장환자의 항산화 체계에 관한 연구)

  • 고영숙;홍영재;정혜연;김수연;이양자
    • Journal of Nutrition and Health
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    • v.35 no.2
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    • pp.229-236
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    • 2002
  • Cataracts are the leading cause of blindness worldwide and are characterized by increased opacity of the lens that significantly diminishes visual acuity. It has been suggested that increased risk of lens opacities are associated with age, exposure to sunlight, diabetes, smoking, and poor nutrition. Antioxidant nutrients have born demonstrated to protect the lens membrane and protein against damage due to oxidative stress. The purpose of this study was to investigate the antioxidant system in the blood of cataract patients. The status of the blood antioxidant system was evaluated based on the levels of antioxidant vitamins and minerals as well as glutathione peroxidase (GSH-Px) and malondialdehyde (M7A) activity in 34 patients with cataracts (17 male and 17 female) and 45 control subjects (20 male and 25 female). After adjusting for age, the results showed significantly lower levels of antioxidant vitamins such as lycopene (M : p < 0.05, F: p < 0.01), zeaxanthin (F: p < 0.01), ${\gamma}$-tocopherol (F: p < 0.01) and ascorbic arid (M: p < 0.05) in the cataract patients than in the control subjects. In contrast, the concentration of cryptoxanthin (F : p < 0.07) showed a significantly higher value in the cataract patients. The serum level of the antioxidant mineral Zn (M : p < 0.01) was found to be significantly lower in the cataract patients while the ratio of cu/zn appeared significantly higher (M : p < 0.05). Significantly higher (M : p < 0.01, F: p < 0.05) concentrations of MDA in serum was found in the cataract patients as compared to the control subjects. GSH-Px activity was significantly lower (F: p < 0.05) in 71e cataract patients. In conclusion, the antioxidant system may play an important roll in cataract creation. Further studies are needed to clarify the mechanisms underlying these findings and to establish preventive measures with an emphasis on antioxidant nutrition for cataract patients.

Research on the technical development by the CAD/CAM System (CAD/CAM시스템을 이용한 기술개발에 대한 연구 (워엄기어 개발을 중심으로))

  • Jeong, Seon-Mo
    • Journal of the Korean Society for Precision Engineering
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    • v.3 no.3
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    • pp.40-71
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    • 1986
  • By developing a computer program for the systematic design of worm gears, the design formulae and tables of AGMA, JGMA, BS and DIN are analized and compared. The computer program can be used on micro-computers. According to the input data of the reduction ratio, the center distance. the driving torque and the material as design parameters, the program calculate the most efficient worm gear dimension. The variation of the design parameters and other empirical coefficients in case of resulting an inadequate design gear dimension can be easily modified throuth the way of interactive method between the user and the monitoring system of computer. A proposal of the standardization of worm gears was made in which a standard module according to the DIN 323 standard series number was applied. For the more exact and effective calculation of the stress concentration and the deformation of gear teeth, a computer program using the boundary element method is also developed. Even the strength of the special gear shape such as Niemann's "Cavex" gear can be calculated in a short CPU-time. The most effort of this study has been layed on the developing a computer program for the correction of a tooth profile and face width which is most important design factor for an exact and wide teeth contacts under loads, especially by great and wide gears. For this purpose were investigated the tooth stiffness, the mesh interferences and the kinematics and the dynamics of gear mesh. The deflection and the deformation of the gear shaft due to the loads acting on gear and shaft were aslo considered. Some examples have shown the sufficient good status of teeth contact in which the correction of the tooth profile and face width were accomplished due to the calculated results.d results.

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Prediction of Ground Condition Changes Ahead of Tunnel Face Using Three-Dimensional Absolute Displacement Analysis (터널 3차원 절대변위 해석기법을 이용한 막장전방지반 예측)

  • Bang, Joon-Ho;Han, Il-Young
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.8 no.2
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    • pp.101-113
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    • 2006
  • Arching effect occurs around the unsupported excavation surface near to tunnel face when a tunnel is excavated in a stable rock mass. If a weak fracture zone exists in front of tunnel face, a displacement occurs between tunnel face and weak fracture zone due to stress concentration. If three-dimensional absolute coordinates (longitudinal, transverse, vertical direction) is measured at tunnel face by geodetic method, the ground change in front of the tunnel face can be predicted by analysing three-dimensional absolute displacement. The purpose of this study is to verify the analysis method of three-dimensional absolute displacement by comparing the trend of displacement ratio at crown and sidewall of tunnel and the influence line/trend line of crown settlement compared with TSP results in the same section.

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