• Preventive Nutrition and Food Science
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• v.11 no.2
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• pp.94-99
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• 2006

This study investigated the protective effect of the butanol (BuOH) fraction from Laminaria japonica (BFLJ) extract on high glucose-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). Freeze-dried L japonica was extracted with distilled water, and the extracted solution was mixed with ethanol then centrifuged. The supernatant was subjected to sequential fractionation with various solvents. The BuOH fraction was used in this study because it possessed the strongest antioxidant activity among the various solvent fractions. To determine the protective effect of the BFLJ, oxidative stress was induced by exposing of HUVECs to the high glucose (30 mM) or normal glucose (5.5 mM) for 48 hr. Cell viability, lipid peroxidation, glutathione (GSH) concentration, and antioxidant enzyme activities such as catalase, superoxide dismutase (SOD), glutathione peroxidase (GSH-px), and glutathion reductase (GSH-re) were measured. Exposure of HUVECs to high glucose for 48 hr resulted in a significant (p<0.05) decrease in cell viability, SOD, GSH-px and GSH-re and a significant (p<0.05) increase in thiobarbituric acid reactive substances (TBARS) formation in comparison to the cells treated with 5.5 mM glucose or untreated with glucose. BFLJ treatment decreased TBARS formation and increased cell viability, GSH concentration, and activities of antioxidant enzymes including catalase, SOD, GSH-px, and GSH-re in high glucose pretreated HUVECs. These results suggest that BFLJ may be able to protect HUVECs from high glucose-induced oxidative stress, partially through the antioxidative defence systems.

• Journal of Pharmacopuncture
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• v.22 no.2
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• pp.109-114
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• 2019

Objectives: Oxidative stress plays a central role in diabetes-induced complications. In the present study, the protevtive effect of Artemisia turanica (A. turanica) was evaluated against diabetes-induced liver oxidative stress and dysfunction. Methods: Fifty male Wistar rats were randomly divided into five groups: control, diabetic, diabetic + metformin, diabetic + A. turanica extract, and diabetic + A. turanica extract + metformin. Experimental diabetes was induced by a single-dose (55 mg/kg, intraperitoneally (ip)) injection of streptozotocin (STZ). Metformin (300 mg/kg) and A. turanica extract (70 mg/kg) were orally administrated three days after STZ injection for four weeks. The levels of malondialdehyde (MDA), total thiol content and superoxide dismutase (SOD) and catalase activities were measured in the liver tissue. Serum glucose concentration, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were also determined. Results: In the diabetic group, serum glucose concentration, serum AST and ALT activities and liver MDA level were significantly higher while tissue total thiol content as well as catalase and SOD activities were lower, compared to the control group. Serum glucose in diabetic rats treated with metformin + A. turanica extract showed a significant decrease compared with the diabetic group. In all the A. turanica extract and metformin treated groups, serum ALT, tissue MDA level, total thiol content and SOD activity significantly improved compared with the diabetic rats. However, treatment of the diabetic rats only with metformin could not significantly change the activities of catalase and AST compared with the diabetic group. Conclusion: These findings suggested that A. turanica extract had a therapeutic effect on liver dysfuncyion and oxidative stress induced by diabetes, that may be probably due to its antioxidant and antiinflammatory effects.

• The Journal of Korean Academy of Prosthodontics
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• v.30 no.4
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• pp.582-610
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• 1992

The long-term success of any dental implant is dependent upon the optimization of stresses which occur during oral function and parafunction. Especially, it has been suggested that there is an unique set of problems associated with joining an osseointegrated implant and a natural tooth with a fixed partial denture. For this particular case, although many literatures suggest different ways to avoid high stress concentrations on the bone surrounding the implant under static and dynamic loading conditions, but few studies on the biomechanical efficacy of each assertion have been reported. The purpose of this investigation was to evaluate the efficacies of clinically suggested methods on stress distribution under static load and shock absorption under dynamic load, using two dimensional finite element method. In FEM models of osseointegrated implant-natural tooth supported fixed partial dentures, calculations were made on the stresses in surrounding bone and on the deflections of abutments and superstructure, first, to compare the difference in stress distribution effects under static load by the flexure of fastening screw or prosthesis, or intramobile connector, and second, to compare the difference in the shock absorption effects under dynamic load by intramobile connector or occlusal veneering with composite resin. The results of this analysis suggest that : 1. Under static load condition, using an implant design with fastenign screw connecting implant abutment and prosthesis or increasing the flexibility of fastening screw, or increasing the flexibility of prosthesis led to the .increase in height of peak stresses in cortical bone surrounding the implant, and has little effect on stress change in bone around the natural tooth. 2. Under static load condition, intramobile connector caused the substantial decrease in stress concentration in cortical bone surrounding the implant and the slight increase in stress in bone around the natural tooth. 3. Under dynamic load condition, both intramobile connector and composite resin veneering showed shock absorption effect on bone surrounding the implant and composite resin veneering had a greater shock absorption effect than intramobile connector.

• Archives of Pharmacal Research
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• v.21 no.3
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• pp.236-240
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• 1998

The effect of thyroid hormones on the hepatic xanthine oxidase activity was studied in rats after the intraperitoneal injections of comthyroid (triiodotyronine:thyroxine=1:4) at 0.3 mg/kg for 3 consecutive days. The aim of this study was to understand the precise mechanism of hyperthyroidism induced by oxidative stress. The concentration of lipid peroxides determined indirectly by the measurement of thiobarbituric acid reactants was increased in comthyroid treated rats. The hepatic glutathione content was decreased in comthyroid injected rat compared to the euthyroid state. It was also observed that the increment of xanthine oxidase activity has a profound role in oxygen radicals generation system in comthyroid treated rat. These findings suggest that the enhanced xanthine oxidase activity and depleting glutathione content in comthyroid treated rats result in pathophysiological oxidative stress including an increment of hepatic lipid peroxidation.

• Laser Solutions
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• v.2 no.2
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• pp.42-51
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• 1999

This study has been performed to investigate into some effects of the output power and traverse speed of laser beam on the microstructures, hardness and fatigue resistance of the ductile iron surface-hardened by $CO_2$ laser defocussed beam. Optical micrographs have shown that with increasing the output power and decreasing the traverse speed, the martensite was coarsened and some retained austenite were appeared in ductile iron. The microstructures of hardening zone were composed of bull's eye and some nodular graphite dissolved structures by the effect of self quenching. Fatigue fracture characteristics of ductile iron have appeared in the high stress and low stress ranges. The fracture initiated at nodular graphites in the surface hardened layer due to the stress concentration caused by a notch effect. The interior graphite nodules were broken away or popped out during crack propagation. Fatigue test has shown that values of fatigue strength considerably increased with increasing output power at a given traverse speed.

• Steel and Composite Structures
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• v.11 no.6
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• pp.435-454
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• 2011

Bonding composite materials to structural members for strengthening purpose has received a considerable attention in recent years. The major problem when using bonded FRP or steel plates to strengthen existing structures is the high interfacial stresses that may be built up near the plate ends which lead to premature failure of the structure. As a result, many researchers have developed several analytical methods to predict the interface performance of bonded repairs. In this paper, a numerical solution using finite - difference method is used to calculate the interfacial stress distribution in beams strengthened with FRP plate having a tapered ends with different thinning profiles. These latter, can significantly reduce the stress concentration. In the present theoretical analysis, the adherend shear deformations are taken into account by assuming a parabolic shear stress through the thickness of both beam and bonded plate. Numerical results from the present analysis are presented to demonstrate the advantages of use the tapers in design of strengthened beams.

• Bulletin of the Society of Naval Architects of Korea
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• v.8 no.1
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• pp.53-66
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• 1971

The effect of reinforced circular hole in a flat plate under general biaxial loading conditions is considered. The reinforcement is achieved by attaching a circular ring of uniform rectangular cross section along the boundary of the hole. This investigation includes a theoretical solution and an experimental conformation. In the theoretical analysis, Gurney's method is used to obtain a solution for the stress distribution and the solution is expressed in a general form, so that it can be applicable to the case of general biaxial loading and general values of Poisson's ratios. In the experimental work a systematic series of photoelastic models, as shown in Fig.5 and Table 1, were analyzed on polariscope. The experimental results were in good agreement with the theoretical ones, as shown in Fig.8 and 9. The conclusions derived are as follows: 1) The theoretical results, given in Eq. $(1){\sim}(5)$, are sufficient in accuracy for the engineering design purpose. 2) The stress concentration factor decreases as the ratio n increases, but not significant beyond n=3. 3) The stress concentration factor increase as the ratio m increases, but not significant below m=0.7.

• Journal of the military operations research society of Korea
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• v.36 no.1
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• pp.91-102
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• 2010

Doubler repair is one of the frequently applied methods to repair cracks which occur at the structure of aircraft weapon systems. The requirement of the doubler repair for some of aging aircraft has increased more recently. However very few researches have been conducted until now. In this paper, the effect of the various doubler repair methods was evaluated. Especially, the relationship between the array of revet and the effect of stress concentration was investigated. To calculate variation of the stress with different repair methods, commercial FEA program was utilized. As a result, it was shown that the main factor for the variation of stress is pitch and number of revet line. Furthermore the optimized array of revet for the specific geometries of doubler was suggested.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.5
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• pp.588-597
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• 2010

We investigated the effect of quercetin on growth and plasma cholesterol level and the effects of quercetin pretreatment (Diet 1, 0%; Diet 2, 0.25%; and Diet 3, 0.5% quercetin) for 30 and 60 days on oxidative stress induced by hypo-osmotic conditions (17.5, 8.75, and 4 psu) in olive flounder. The weights of flounder were higher with Diet 3 than with Diet 1 and 2, which indicated that a high concentration (Diet 3) of quercetin was very effective in growth. Total cholesterol levels were lower with Diets 2 and 3 than with Diet 1, leading us to hypothesize that quercetin removed low-density lipoproteins from circulation and thereby reduced total cholesterol. To understand the antioxidant role of quercetin, we measured the mRNA expression and activities of superoxide dismutase (SOD) and catalase (CAT) and the $H_2O_2$ concentration in quercetin-treated flounder exposed to osmotic stress. The $H_2O_2$ concentration and the SOD and CAT expression and activity levels were lower in flounder fed with Diets 2 and 3 than with Diet 1, suggesting that quercetin directly scavenges reactive oxygen species to reduce oxidative stress. Furthermore, the plasma lysozyme activity and osmolality were higher with Diets 2 and 3 than with Diet 1, indicating that quercetin increases immune function and helps to maintain physiological homeostasis. Plasma cortisol was lower with Diets 2 and 3 than with Diet 1, suggesting the quercetin protects against stress. These results indicate that quercetin has hypocholesterolemic and antioxidant effects, increases immune function, and acts to maintain physiological homeostasis.

• The Journal of Korean Academy of Prosthodontics
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• v.18 no.1
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• pp.37-47
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• 1980

The purpose of this study was to investigate the stresses in different proximal margins and to measure, quantitatively, the effect of different modifications in the design of preparations on the stresses using two-dimensional photoelasticity. Photoelastic stress analysis is based on the phenomenon, exhibited by most transparent solids, of becoming birefringent, or doubly refracting, when strained. Two birefringent materials were used in this study, PSM-1 and PSM-5 in .standard sheet ($10'{\times}10'{\times}\frac{1}{4}'$ thickness), PSM-1(polyester) was used for constructing the substructure, and PSM-5(epoxy resin) was used in making the restorations to be investigated. Two birefringent materials were used in the construction of composite photoelastic model. Seven variable models were constructed. The peripheral dimensions of all model were constant and the models represent an occlusomesial section of a lower posterior molar. Model 1 represents the knife edge margin (shoulderless), Model 2 represents the chamfer, Model 3 represents a rounded shoulder(no sharp angle between the axial wall and gingival floor), Model 4 represents a flat shoulder (axial wall is a $90^{\circ}$ angle to the gingival wall), Model 5 represents $+15^{\circ}$ angulation, Model 6 has a $-15^{\circ}$ angulation, and Model 7 is the same as Model 4 except that it has a $45^{\circ}$ bevel. Improved artificial stone was used to represent dental cement in luting the composite photoelastic model. Static loading procedures(100 pounds) were used at preplanned sites. The results were as follows; 1. The stresses in the proximal portion of all tested models were compressive in nature when the proximal shoulders were loaded vertically on the same proximal marginal ridge. 2. The round and chamfered preparations were the optimum designs in proximoocclusal restorations. They showed the lowest stress concentration factor, i.e. 2.16 and 2.23, respectively. The knife edged shoulder had the highest value, K=5.39. Round type shoulder geometry experiments reduced the stress concentration factor (S.C.F.) 3. The gingival portion of proximal shoulder geometry was a critical location for stress concentration.