• 한국미생물·생명공학회지
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• 제10권2호
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• pp.117-122
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• 1982

전보$^{(1,2)}$ 에서 기술한 경기도 소요산분근의 토양으로부터 분리된 강한 항진인성 물질 t-cinnamamide와 미지의 물질을 생성하는 streptomyces속 No.297은 균의 형태적, 생리적및 배양상의 특성을 조사하여 동정한 결과 Streptomyces griseorubiginosus var. soyoensis도 동정되었다.

• 한국미생물·생명공학회지
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• 제37권4호
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• pp.383-388
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• 2009

토양으로부터 세포외로 xylanase를 분비 생산하는 방선균 YB-914가 분리되었으며, 형태, 배양, 생화학적 특성을 조사한 결과 Streptomyces 속 균주로 확인되었다. 분리균의 배양상등액에 존재하는 xylanase는 pH 5.5과 $55^{\circ}C$의 반응조건에서 반응성이 가장 높았으며, pH 4.5~7.0 범위에서 최대활성의 80% 이상을 나타냈다. Xylanase의 생산을 위한 배지를 최적화하기 위해서 G.S.S 배지성분을 여러 종류의 탄수화물로 대체하였다. Oat spelt xylan, corn cob xylan, 밀기울 및 유당과 같은 탄수화물은 Streptomyces sp. YB914의 xylanase 생산성을 증가시키는 것으로 확인되었으며, galactose와 arabinose는 효소 생산을 크게 억제하였다. Oat spelt xylan(1%)와 유당(1.5%)을 함유한 변형배지에서 xylanase의 최대생산성이 48 U/mL로 확인되었다.

• 한국식품영양학회지
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• 제11권3호
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• pp.340-346
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• 1998

항생제에 대한 내성균의 출현은 매년 급격하게 증가되고 있으며 이에 대한 약제 개발이 요구되고 있다. 특히 메치실린내성 황색 포도상구균(MRSA)은 여러 항생물질에 대하여 다제내성을 나타내므로 감염증치료에 어려운 문제점으로 남아 있다. 이러한 감염증치료에 사용할 목적으로 임상 가검물에서 분리한 MRSA를 시험균으로 사용하여 항균 활성이 강한 방선균인 YSK-681을 선별하였다. 균주 동정을 위하여 선별된 YSK-681을 형태적, 배양학적, 생리적, 화학적 분석을 하였으며 41개의 단위 항목을 조사하여 TAXON program으로 수리 동정한 결과 주군집 29개에 속하는 Streptomyces lydicus로 동정하였다.

• 한국환경농학회지
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• 제30권3호
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• pp.346-356
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• 2011

BACKGROUND: Chemical fungicides not only may pollute the ecosystem but also can be environmentally hazardous, as the chemicals accumulate in soil. Biological control is a frequently-used environment-friendly alternative to chemical pesticides in phytopathogen management. However, the use of microbial products as fungicides has limitations. This study isolated and characterized a three-antifungal-enzyme (chitinase, cellulase, and ${\beta}$-1,3-glucanase)-producing bacterium, and examined the conditions required to optimize the production of the antifungal enzymes. METHOD AND RESULTS: The antifungal enzymes chitinase, cellulase, and ${\beta}$-1,3-glucanase were produced by bacteria isolated from an sawmill in Korea. Based on the 16S ribosomal DNA sequence analysis, the bacterial strain AM50 was identical to Streptomyces sp. And their antifungal activity was optimized when Streptomyces sp. AM50 was grown aerobically in a medium composed of 0.4% chitin, 0.4% starch, 0.2% ammonium sulfate, 0.11% $Na_2HPO_4$, 0.07% $KH_2PO_4$, 0.0001% $MgSO_4$, and 0.0001% $MnSO_4$ at $30^{\circ}C$. A culture broth of Streptomyces sp. AM50 showed antifungal activity towards the hyphae of plant pathogenic fungi, including hyphae swelling and lysis in P. capsici, factors that may contribute to its suppression of plant pathogenic fungi. CONCLUSION(S): This study demonstrated the multiantifungal enzyme production by Streptomyces sp. AM50 for the biological control of major plant pathogens. Further studies will investigate the synergistic effect, to the growth regulations by biogenic amines and antifungal enzyme gene promoter.

• 약학회지
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• 제44권5호
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• pp.478-487
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• 2000

This study was carried out to find new anti-tumor agent producing microbe and to characterize the anti-tumor agent produced from the microbe. Purified compound that has a high cytotoxicity against tumor cell-lines could be obtained from the broth culture filtrates of Streptomyces sp.409 strain isolated from soil in Korea. The in vitro cytotoxicity the in vivo evaluation of acute toxicity the safety assessment of the anti-tumor compounds and the taxonomic characteristics of the anti-tumor agent were measured. The antitumor compound 1 and 2 were obtained from the broth culture filtrates of Streptomyces sp.409 strain. The cytotoxicity of the compound 1 against tumor cell-line P388D$_1$ showed almost 4.5 times higher than that of adriamycin. However in the cytotoxicity against normal cell line Vero E6, adriamycin showed adversely 4 times higher than the compound 1 ($IC_{50}$/ value: 228.7 $\mu\textrm{g}$/$m\ell$). In comparison study with compound 1 and compound 2 in the in vitro cytotoxin productivity against tumor cell lines, $IC_{50}$/ value of the compound 1 was 0.25 $\mu\textrm{g}$/$m\ell$ in tumor cell line P388D$_1$and 0.53 $\mu\textrm{g}$/$m\ell$ in tumor cell-line L1210, and that of the compound 2 was 7.18 $\mu\textrm{g}$/$m\ell$ and 35.71 $\mu\textrm{g}$/$m\ell$, respectively; LD$_{50}$ value of the compound 1 in the in vivo acute toxicity in mice was 22.62 $\mu\textrm{g}$/kg body weight. These results suggest that compound 1 purified from Streptomyces sp. 409 has anti-tumor activity and will be developed as an anti-tumor drug.g.

• 미생물학회지
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• 제34권3호
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• pp.162-168
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• 1998

기름-물 유상액의 탈유화능이 있는 Streptomyces sp. 8321을 분리하여 이의 탈유화 특성을 조사하였다. 이 방선균의 탈유화능은 포자에 기인하였으며, 배양기간에 따라 포자표면의 소수성이 증가하였고, 이에 따라 탈유화능이 증가함을 보였다. 8일간 고체배지에서 배양한 Streptomyces sp. 8321의 포자용액이 kerosene-0.2% Triton X-100(2:1) 표준 유상액 3 ml을 완전 탈유화 시키는 농도는 $1.1{\times}10^8spores/ml$였다. 이 포자용액의 탈유화능을 유기상 종류 및 유화제 종류에 따라 조사해본 결과, 저점성의 유기상에서는 n-hexadecane 및 diesel 등 탄소수가 높은 것이 쉽게 탈유화 되었고, 고점성 유상액의 탈유화는 24시간 후에도 20-30%만이 일어났다. Corexit, Finalsol, BP series의 유화제와 kerosene으로 이루어진 O/W 유상액은 1분 이내에 완전 탈유화가 일어났으며, Corexit 7664, 8667, Triton X-100 및 Tween 80으로 이루어진 O/W 유상액은 이들 유화제의 농도가 증가함에 따라 탈유와 속도가 감소하였다. Seagreen으로 이루어진 W/O 유상액의 경우 $t_{1/2}$이 24시간 이상으로 나타나 이 균주의 탈유화능은 O/W 유상액에 효과적임을 보여주었다.

• Mycobiology
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• 제46권2호
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• pp.138-146
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• 2018

Two-hundred and fifty-five strains of actinomycetes isolated from soil samples were screened for their antagonistic activities against four well-known wood decay fungi (WDF), including a brown rot fungus, Gloeophyllum trabeum and three white rot fungi Donkioporia expansa, Trametes versicolor, and Schizophyllum commune. A dual culture assay using culture media supplemented with heated or unheated culture filtrates of selected bacterial strains was used for the detection of their antimicrobial activity against four WDF. It was shown that Streptomyces atratus, S. tsukiyonensis, and Streptomyces sp. greatly inhibited the mycelial growth of the WDF tested compared with the control. To evaluate the biocontrol efficacy of S. atratus, S. tsukiyonensis, and Streptomyces sp., wood blocks of Pinus densiflora inoculated with three selected Streptomyces isolates were tested for weight loss, compression strength (perpendicular or parallel to the grain), bending strength, and chemical component changes. Of these three isolates used, Streptomyces sp. exhibited higher inhibitory activity against WDF, especially G. trabeum, as observed in mechanical and chemical change analyses. Scanning electron microscopy showed that cell walls of the wood block treated with Streptomyces strains were thicker and collapsed to a lesser extent than those of the non-treated control. Taken together, our findings indicate that Streptomyces sp. exhibits the potential to be used as a biocontrol agent for wood decay brown rot fungus that causes severe damage to coniferous woods.

• Journal of Applied Biological Chemistry
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• 제56권2호
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• pp.119-129
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• 2013

Thirteen different Streptomyces isolates were evaluated for their ability to produce keratinase using chicken feather as a sole carbon and nitrogen sources under solid state fermentation (SSF). Streptomyces sp. NRC 13S produced the highest keratinase activity [1,792 U/g fermented substrate (fs)]. The phenotypic characterization and analysis of 16S rDNA sequencing of the isolate were studied. Optimization of SSF medium for keratinase production by the local isolate, Streptomyces sp. NRC13S, was carried out using the one-variable-at-a-time and the statistical approaches. In the first optimization step, the effect of incubation period, initial moisture content, initial pH value of the fermentation medium, and supplementation of some agro-industrial by-products on keratinase production were evaluated. The strain produced about 2,310 U/gfs when it grew on chicken feather with moisture content of 75% (w/w), feather: fodder yeast ratio of 70:30 (w/w), and initial pH 7 using phosphate buffer after 8 days. Based on these results, the Box-Behnken design and response surface methodology were applied to find out the optimal conditions for the enzyme production. The corresponding maximal production of keratinase was about 2,569.38 U/gfs.

• 한국미생물·생명공학회지
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• 제30권4호
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• pp.332-338
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• 2002

토양으로부터 세포외로 $\alpha$-galactosidase를 분비 생산하는 방선균 YB-4가 분리되었으며, 분리균의 배양ㆍ형태ㆍ생리적 특성을 조사한 결과 Streptomyces 속 균주로 확인되었다. 분리균의 배양상등액으로부터 부분정제된 $\alpha$-galactosidase를 조효소액으로 사용하였을 때 para-nitrophenyl-$\alpha$-D-galacto-pyranoside는 pH 6.0과 6$0^{\circ}C$의 반응조건에서 가장 잘 분해되었으며, 조효소액을 pH 4.0에서 pH 10.0범위에서 1시간 이상 방치한 후에도 약 90% 이상의 $\alpha$-galactosidase 활성을 유지하였다. 또한 분리균이 생산하는 $\alpha$-galactosidase는 melibiose, raffinose와 stachyose와 같은 저당류를 가수분해 할 수 있으며 분해산물로 galactose를 방출하는 것으로 보아 $\alpha$-1,6 결합을 분해한 것으로 확인되었다.

• Journal of Microbiology
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• 제45권1호
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• pp.6-10
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• 2007

Culture broth of a streptomycete isolate, Streptomyces sp. CS684 showed antibacterial activity on methicilin resistant Staphylococcus aureus (MRSA) and vancomycin resistant enterococci (VRE). Among purified substances from the organism, CSU-1, which is active against MRSA and VRE, is a $C_{37}H_{62}O_{12}Na\;(M^+,721.3875)$, and identified as laidlomycin. The anti-MRSA and anti-VRE activity of CSU-1 was stronger than oxacillin and vancomycin. Phylogenetic analysis showed that strain CS684 is very similar to Streptomyces ardus NRRL $2817^T$, whereas the ability of Streptomyces sp. CS684 to produce laidlomycin was shown to be unique.