• Journal of Animal Science and Technology
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• v.49 no.6
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• pp.847-856
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• 2007

This study was conducted to determine the effect of cryoprotectants(sugar, sorbitol, polyphosphate) on quality properties of chicken breast surimi manufactured by pH adjustment(pH 11.0) during frozen storage. Final surimi was divided into experimental units to which the following treatments were randomly assigned: C(Alaska pollack surimi, two times washing, 4% sugar＋5% sorbitol＋0.3% polyphosphate additive); T1(chicken breast surimi, 0.3% polyphosphate additive); T2(chicken breast surimi, 5% sorbitol ＋0.3% polyphosphate additive); T3(chicken breast surimi, 4% sugar＋5% sorbitol＋0.3% polyphosphate additive). All amino acid contents of control were higher than those of all treatments, while T2 was higher in amino acid contents among the treatments. Shear force of all treatments were higher than that of control, but the breaking force, deformation and gel strength were lower. The TBARS(thiobarbituric acid reactive substances) and VBN(volatile basic nitrogen) values of all treatments were lower than those of control, The TBARS values of all treatments were increased with increased storage period. In sensory evaluation, the score of appearance, meat color and overall acceptability of control were higher than those of all treatments, but aroma, juiciness and tenderness were lower than those for all treatments.

• Korean Journal of Poultry Science
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• v.35 no.2
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• pp.171-176
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• 2008

Animal welfare advocates, claim that the induced molting by fasting be avoided. This study was conducted to evaluate the effect of molting induced by various feeding methods on productivity and egg quality in laying hens. We used 400 flocks of 60-week-old leghorn laying hens in four treatments(five replicates of 20 hens each): fasting method (C), feeding single corn grain diet (T1), feeding single wheat bran diet (T2) and feeding single alfalfa meal diet (T3). As the result of the experiment, egg production and daily egg mass significantly decreased only in T1 compared with the control during the $1{\sim}4th$ week after the secondary egg laying (P<0.05). Although the amount of feed intake was significantly less in T1 group during the $1{\sim}4th$ week compared to the control, no significant difference was detected during the total period (P>0.05). In addition, no significant difference of feed conversion was observed between treatment groups. In terms of egg quality, the egg shell thickness was significantly improved in T1 group than the control group by feeding only corn at the 10th and 14th week after the secondary egg laying (P<0.05), but the significant decrease was observed at the corn (T1) and wheat bran (T2) fed treatment groups than the control group at the 26th week of the experiment (P<0.05). The eggshell strength and haugh unit did not show any difference by the molting methods. Egg yolk color was significantly decreased in T1 and T2 group than the control group at the 6th week (P<0.05). However, T1 and T2 group resulted to show significantly high egg yolk color at the 18th week of the experiment (P<0.05). As the result of the experiment, no large difference was observed in the productivity by the feeding molting method and by the fasting induced molting method. In addition, the single diet fed feeding induced molting method by using alfalfa revealed to show more satisfactory trend than the corn or wheat bran single diet fed feeding induced molting methods even if no statistically significant difference was found in terms of egg productivity.

• Korean Journal of Poultry Science
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• v.30 no.4
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• pp.275-280
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• 2003

This study conducted to investigate the effect of dietary germanium biotite by protein level in laying hen diets. One hundred forty four, 51 weeks old ISA brown commercial layer, were used in experiment. Dietary treatments were 1) low protein diet(LPD), 2) high protein diet(HPD), 3) LPD-GB(LPD + 1.0% germanium biotite) and 4) HPD-GB(HPD + 1.0% germanium biotite). Henday egg production tended to be increased as the concentration of protein in diets increased with significant difference(P<0.01). Egg weight tended to decrease by increasing of supplementation germanium biotite in the diets(P<0.01). Egg shell breaking strength was not influenced by germanium biotite supplementation(P>0.05). Large band of egg decrease as increasing of supplementation germanium biotite in the diets(P<0.02). Sharp and middle band of egg were not influenced by germanium biotite supplementation. Egg yolk index tended to decrease as increasing of supplementation germanium biotite in the diets(p<0.01). Fecal propionic acid(P<0.01) and butyric acid(P<0.03) were decrease as the concentration of germanium biotite in the diet was increased. Also, butyric acid increased as the concentration of protein in diets increased with significant difference(P<0.02). Supplementation germanium biotite in the diet reduced the fecal acetic acid(P<0.01). Fecal $NH_3$-N of hens fed HPD-GB diet was decreased(P<0.05) compared to that LPD-GB diet. In conclusion, germanium biotite supplementation to layer diets can reduce fecal volatile fatty acid compabebts.

• Journal of the Mineralogical Society of Korea
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• v.22 no.3
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• pp.249-259
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• 2009

The objective of this research was to find out the physical properties, such as dispersion and coagulation, of soil minerals depending on the types and concentrations of the cations in aqueous solution. Hwangto samples were obtained from 90 to 130 cm from surface at Jangdong-ri, Donggang-Myon, Naju, Chonnam Province. The clay fraction (< $2\;{\mu}m$) was separated by sedimentation method from the bulk soils. Both Hwangto and clay fractions, and the same samples after removal of amorphous and crystalline iron oxides were used in this experiment. The effect of 4 cations ($Na^+$, $K^+$, $Mg^{2+}$, $Ca^{2+}$) and their concentrations on settling speed and basal spacing of the minerals were observed to examine the physical properties of the soil and clay minerals. Hwangto mainly consisted of quartz, and the clay fractions consisted of kaolinite, illite, and vermiculite. The bulk soils contained 16.3 mg/kg of amorphous iron oxides and 436 mg/kg of crystalline iron oxides. Clay fractions were dispersed better than bulk soils due to their smaller particle size than that of the bulk samples in the aqueous solution. The bulk and clay samples were dispersed better when iron oxides were removed because of coating of minerals by the iron oxides. Clay minerals were settled faster as the charge and the concentration of cations added increased. The d-spacing of kaolinite and illite did not change when 4 types of cations were added. The d-spacing of vermiculite showed $14.04\;{\AA}$ when divalent cations were added while that of vermiculite showed $13.9\;{\AA}$ when monovalent cations were added. It may be attributed to the hydration radii of cations. This study indicated that both coating of iron oxides on minerals and types and concentrations of cations affect dispersion of minerals in solution and d-spacing of expanding clay minerals such as vermiculite.

• Journal of Animal Science and Technology
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• v.51 no.4
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• pp.295-306
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• 2009

This experiment was conducted to investigate the effects of dietary calcium (Ca) and vitamin $D_3$ metabolite on eggshell quality and hatching egg production in aged egg-type breeder hens. A total of five hundred and forty 60-week-old Hy-Line Brown breeder hens were randomly allocated to nine treatments in a $3\times3$ factorial design to determine the effects of dietary Ca levels (3.3, 3.9, and 4.5%), combined with three levels of 25-hydroxycholecalciferol [25-$(OH)D_3$ 0, 75, and $150{\mu}g/kg$], on eggshell quality and egg production for 10 weeks. There were significant Ca and 25-$(OH)D_3$ effects (P<0.01) on egg production rate. There was a linear (P<0.01) increase in eggshell strength and thickness with increasing dietary Ca and 25-$(OH)D_3$ levels. Dietary 25-$(OH)D_3$ had a significant effect (P<0.05) on hatchability of egg although fertility was not influenced by dietarylevels of Ca and 25-$(OH)D_3$. Dietary Ca levels affected Ca content in tibia (P<0.05). These results indicated that relatively high levels of dietary Ca in combination with 25-$(OH)D_3$ had a beneficial effect on improving eggshell quality and reproductive performance in aged egg-type breeder hens.

• Journal of the Korean Chemical Society
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• v.44 no.5
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• pp.403-409
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• 2000

The formation and dissociation rates of $Zn^{2+}$ Complexes with l,4,7,10-tetraaza-13,16-diox-acyclooctadecane-N,N',N",N'"-tetraacetic acid (1), 1,4,7,10-tetraaza-13,16- dioxacyclooctadecane-N,N',N",N'"-tetramethylacetic acid (2), and 1,4,7,10-tetraaza-13,16- dioxacyclooctadecane-N,N',N",N'"-tetrapropionic acid(3) have been measured by stopped-flow and conventional spectrophotometry. Observations were made at 25.0$\pm$0.1 $^{\circ}C$ and at an ionic strength of 0.10 M NaClO$_4$. The formation reactions of $Zn^{2+}$ ion with 1 and 2 took place by the rapid formation of an intermediate complex (ZnH$_3L^+$) in which the $Zn^{2+}$ ion is incompletely coor-dinated. This might then lead to be a final product in the rate-determining step.ln the pH range 4.76-5.76, the diprotonated (H2L2-) form is the kinetically active species despite of its low concentration. The stability con-stants (log$K_{(ZnH$_3$3$L^+$)}$) and specific water-assisted rate constants (koH) of intermediate complexes have been deter-mined from the kinetic data. The dissociation reactions of $Zn^{2+}$ complexes of 1,2, and 3 were investigated with $Cu^{2+}$ ions as a scavenger in acetate buffer. All complexes exhibit acid-independent and acid-catalyzed con-tributions. The effect of buffer and $Cu^{2+}$ concentration on the dissociation rate has also been investigated. The ligand effect on t dissociation rate of $Zn^{2+}$ complexes is discussed in terms of the side-pendant armsand the chelate ring sizes of the ligands.

• Korean Journal of Agricultural Science
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• v.34 no.1
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• pp.53-60
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• 2007

A layer feeding trial was conducted for 10 weeks to investigate the effects of the addition of corn distillers dried grains with solubles (DDGS) to layer diets on the laying performance, egg qualities, and yolk fatty acid composition. Nine hundred Hyline Brown layers, 24 weeks of age,were randomly allotted to 20 replicate laying cages, 45 birds per replicate. There were four diet treatments (0, 10, 15, and 20% DDGS), and five replicates per treatment. All experimental diets were prepared to contain iso-protein (17%) and iso-calorie (TMEn 2,780 kcal/kg). The use of DDGS up to 20% level in layer diets did not exert any influence on feed intake, laying rate, total egg mass, mean egg weight, and feed conversion ratio. DDGS did not exert any influence in weight of egg, breaking strength, and color of eggshell. The albumen height and Haugh unit was not influenced by DDGS addition. The yolk color was significantly increased by DDGS supplementation. As the DDGS level increased, the oleic acid content decreased, and the linoleic acid increased (P<0.05). The degree of saturation of yolk fatty acids was not affected by dietary DDGS. The inclusion of DDGS up to 20% in layer diets resulted in the decrease of feed cost per kg without any effect in the laying performance. In conclusion, the use of DDGS up to 20% level in layer diets could replace corn and soybean meal without any harmful effect on the laying performances.

• Korean Journal of Food Science and Technology
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• v.26 no.4
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• pp.442-447
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• 1994

Effect of different mixing ratio of non-glutinous and glutinous rice on physical and rheological properties of extrudate prepared in a single screw extruder were examined. The extrusion conditions in term of screw speeds, moisture content and die temperature were 258 rpm, 18% and $120^{\circ}C$, respectively. The resisdence time distribution of the most of materials were within 30 second and small portion of them went up to 80 second. The expansion ratio was the highest value (2.93) for 70% of glutinous rice in the mixture, while the lowest value for 100% of non-glutinous rice. Breaking strength was in the range between 1,051g and 1,117g for $10{\sim}20%$ of glutinous rice in the mixture, while the lowest value (737g) for 80%r of glutinous rice. As the amount of glutinous rice increased, L and a values were increased and b value was decreased. The uncooked cold paste viscosity had 400 B.U. for 100% non-glutinous rice , while no peak for the 100% glutinous rice. As the amount of glutinous rice increased up to 100%, the water absorption index (WAI) was decreased, while water solubility index (WSI) was increased. The rheological properties of extrudate were accounted by the law of Oswald. The flow behavior index of extrudate was less than 1.0, which showed pseudoplastic behavior. Yield stress was the highest value for 20% of glutinous rice in the mixture and the lowest value for $80{\sim}100%$ of glutinous; rice. Number of air cell was between 128 and 159 for $80{\sim}100%$ of glutinous rice in the mixture, while $81{\sim}84%$ for $0{\sim}20%$ of glutinous rice. The degree of shapefact was increased more when the mixtures of glutinous and non-glutinous rice was used than when glutinous or non-glutinous rice was only used.

• Tuberculosis and Respiratory Diseases
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• v.40 no.6
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• pp.683-693
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• 1993

Background: During movement the major inputs to nervous system come from firstly the muscle and joint to maintain posture and motion and secondly the chemoreceptors and baroreceptors to adjust the cardiovascular and respiratory function. Their complex relationships are generally studied for many years but the direct relation between the joint and respiratory system is not studied thoroughly until now. So this experiment was performed to determine whether the natural movement of knee joint can cause the enhancement of respiratory function by observation of the changes of respiratory rate, phrenic nerve activity and inspiratory neuron activity during the stimulation of knee joint in cat anesthetized with $\alpha$-chloralose. Method: Twenty six male adult cats were used and the extracelluar recording using bipolar platinum electrode and carbon filament electrode was done to record the changes in the activities of phrenic nerve and inspiratory neuron movement of knee joint, injection of chemicals into the joint cavity and electrical stimulation of articular nerve were done. Results: The 60 Hz. could not but 120 Hz. flexion-extension movement of knee joint increased respiratory rate(R.R.), tidal neural activity(TNA) and minute neural activity(MNA). Intra-articular injection of lactate could not increase R.R. but significantly increase TNA and MNA which represented the enhanced respiratory function. Injection of potassium chloride showed similar effects with the case of lactate but the duration of effect was shorter. The electrical stimulation of medial articular nerve with IV strength which could activate only group I and II afferents showed increased TNA and MNA during stimulation but 20 V stimulation which could activate all the afferents increased all the respiratory parameters. The changes of inspiratory neuron activity by knee joint stimulation was similar to that of phrenic nerve. Conclusion: The respiratory center could be directly stimulated by the activation of group I and II articular afferents and it seemed that the magnitude of the respiratory center enhancement is proportional to the amount of sensory information from the knee joint. These facts might suggest that the respiratory function could be enhanced even by the normal movement of knee joint.

• Journal of Dental Rehabilitation and Applied Science
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• v.27 no.2
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• pp.161-174
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• 2011

The purpose of this study was to evaluate the effect of ethanol addition on efficacy of two-step total-etch adhesive under over-wet condition by measurement of remaining volatile part (RVP), microtensile bond strength (${\mu}TBS$), and degree of conversion (DC). Two-step total-etch adhesive, Optibond Solo Plus (Kerr, Orange, USA), was used. Experimental groups were divided into 8 groups: Group 1 (only 10 ${\mu}l$ adhesive), Group 2 (mixture of 3 ${\mu}l$ distilled water and 10 ${\mu}l$ adhesive), From Group 3 to Group 8 (mixture of 3 ${\mu}l$ distilled water, 10 ${\mu}l$ adhesive, and ethanol added in 1 ${\mu}l$ increment from 1 ${\mu}l$ to 6 ${\mu}l$). The mixtures were placed on slide glass and evaporated for 10 s, 30 s, and 60 s by air-drying. The weight of RVP was measured by precision weight. Same procedures were performed for ${\mu}TBS$ test and measurement of DC. The condition of mixed solution was observed under light microscope. For RVP weight, the weights of experimental groups except for group 1 decreased with the increase of air-drying time (p<0.05). The DC increased with the increase of air-drying time in only group 5 and 6 (p<0.05). The ${\mu}TBS$ increased with the increase of air-drying time in group only 5, 6, and 7 (p<0.05). The phase separation was examined and water blisters were diminished with the increase of air-drying time in group 5, 6, 7, and 8. Within the limits of this study, ethanol additionally applied to adhesive decreased RVP and increased DC and ${\mu}TBS$ under over-wet condition. It was shown that the addition of ethanol to two-step total-etch adhesive under over-wet condition would remove water and increase the efficacy of adhesive.