• Korean Journal of Environmental Biology
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• v.23 no.4
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• pp.383-389
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• 2005

The effect of different mulching materials on mungbean production was studied. The general objective was to assess the ecological effects of mulching materials in sustainable mungbean production. Specifically, the study aimed to determine the effects of different mulching materials on the chemical, physical and biological soil properties, on weed control and yield, and to identify mulching materials that are environmentally friendly in mungbean production. The experiment was conducted at the Fruit and Vegetables Seeds Center, Science City of $Mu/tilde{n}oz$, Nueva Ecija, Philippines from May to July 2004. The initial soil chemical properties were: pH of 6.4, 2.0 percent organic matter content, 0.10 percent total nitrogen, 22 ppm phosphorus, and 370 ppm available potassium. The soil microbial loads were $8\times10^4\;CFU\;g^{-1}$ for bacteria and $14\times10^4\;CFU\;g^{-1}$ for fungi. Mushroom spent mulch increased soil organic matter with an average of 3.13 percent, nitrogen with an average of 0.16 percent and the highest number of bacterial count with $3.4\times10^8\;CFU\;g^{-1}$. Use of mulch, except rice straw mulch, generally increased mungbean yield. The best mulching material for high yield production of mungbean was black polyethylene plastic film, although environmentally unfriendly.

• Journal of Animal Science and Technology
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• v.49 no.5
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• pp.667-676
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• 2007

This study was conducted to determine effects of cellulolytic microbes inoculation to sawdust-based spent mushroom substrate(SMS) during deepstacking on fermentation parameters, total microbial counts and cellulolytic enzyme activity and to on SMS nutrients utilization by sheep. For sheep metabolism trials, six sheep(ram, average 54.8kg) were fed a Control diet(70% concentrates, 15% rice straw and 15% SMS with no microbial treatment on a dry basis) and a Treatment diet(the same diet including SMS with a microbial treatment) for 2 trials. Spent mushroom substrates with or without a microbial(4 strains including 1 strain of Enterobacter ludwigii, 1 strain of Bacillus cereus and 2 strains of Bacillus subtillis) treatment (1% of SMS on wet basis) were deepstacked for 7 days. The internal temperatures in 1.2 M/T of SMS deepstacks reached to 50±5℃ within 7 days of storage. Total microbial counts remarkably decreased (P<0.05) with a deepstacking process and were not affected(P>0.05) by the microbial treatment. For fibrolytic enzyme activity, CMCase and xylanase activities were decreased(P<0.05) by a deepstacking process. After deepstacking, the microbial treatment showed about 2.5-times higher(P<0.05) for CMCase activity and about 4-times higher(P<0.05) for xylanase activity than those of the Control. Activities of ligninolytic enzymes such as laccase and MnP were not affected by the microbial treatment. The sheep fed the microbially treated SMS diet had a tendency of greater total tract digestibilities of ash(P=0.051), NFE (P=0.071), hemicellulose(P=0.087) and NDF(P=0.096) than those fed the untreated SMS diet. Nitrogen balance of sheep was not affected(P>0.05) by feeding of microbially treated SMS. Accordingly, these results indicate that cellulolytic microbes inoculation during deepstacking of SMS may improve the bio- utilization of SMS by sheep.

• The Korean Journal of Mycology
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• v.9 no.3
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• pp.129-132
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• 1981

Thle experiments were carried out to select the highest yield and good quality of the oyster mushroom (Pleurotus sp.) strain for brooding material sources. The strains used covered 6 from 4 countries, No. 2063, 2078 and 2093 were collected from Korea, No. 2068 introduced from Japan, No. 2091 from Germany and No. 2095 from Taiwan. Some characteristics of the strains are as follows. 1) The optimum temperature for strains, 2091, 2093 and 2063 of P. ostreatus was $30^{\circ}C$, it ranged from 25 to $30^{\circ}C$ for the others. 2) The optimum moisture content were 70% for strain 2068, 80% for strains 2093, 2095 and 2091, 75% for other strains. 3) The optimum pH of the mycelial growth were at pH 6.0 for strains 2093 and 2068, pH 6.0 and pH 8.0 for other strains. 4) The days from spawning to budding was the shortest in strain 2095 and the yield was the highest in 2091, $64.4kg/3.3m^2$on the rice straw cultivation.

• The Korean Journal of Mycology
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• v.2 no.1
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• pp.21-24
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• 1974

Present experiments were conducted to determine the possibility of cultivation of tropical and subtropical saprophytic fungus, Volvariella volvacea (Bull. ex Fr.) Sing. under Korean climatic conditions and the-possibility of applying the method of indoor cultivation which was modified from the cultivation method of the common mushroom, Agaricus bisporus (Lange) Sing.. The results obtained were as follows: 1. In conventional outdoor cultivation, the adequate cultivation period of this fungus was from around the end of June to the end of August. 2. When rice straws submerged in water for 5 days were peak-heated for 6 days in a mush room house, the yield of the fungus was higher than that obtained from the rice straws peak-heated immediately after outdoor composting. 3. The highest yield, 20.49kg per 100kg rice straws, was obtained from the water-soaked and peak-heated rice straws which were cased with a 2 cm layer of loam at 7 days after spawning.

• The Korean Journal of Mycology
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• v.27 no.1 s.88
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• pp.10-14
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• 1999

A single phase composter was constructed by modifying the conventional mixer of sawdust for the cultivation of oyster mushroom Pleurotus ostreatus. The machine was designed on the basis of 3-phase-1 system which was controlled in prewetting, pasteurization and fermentation processes. In composting 200 kg of straw and cotton waste in the machine, it took 20 minutes in prewetting step and also to hours at $65^{\circ}C$ in pasteurization process. Postfermentation by aerothermophiles was completed by treating the compost at $45^{\circ}C-50^{\circ}C$ for 48 hours which was shorten 24 hours from the conventional method. In the postfermentation at high temperature, forced aeration and/or vigorous mixing process(es) played a great role in the improvement of spawn quality. The growth of mycelium of oyster mushroom was excellent in the culture combinated with 3 parts of surface inoculation and 7 parts of mechanical mixing.

• The Korean Journal of Mycology
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• v.12 no.2
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• pp.53-58
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• 1984

This experiment was performed to select the most suitable species of oyster mush­room for cultivation during summer season in Korea. The selection was made based upon mycelial growth on the ricestraw substrates, fruiting under hot weather conditions during the summer season in Korea and potential high productivity using six cultures from South-east Asia. The newly selected strain of Pleurotus sajor-caju (ASI 2070) grew well on the rice straw substrates and produced the higher yields of sporophores than other cultures tested. The primordia of the mushroom were formed between 10 to $35^{\circ}C$. The optimal temperature for fruiting ranged from 20 to $25^{\circ}C$.

• Korean Journal of Plant Resources
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• v.10 no.2
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• pp.175-182
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• 1997

This study was carried out to investigate the effect of several organic fertilizers on the growth characteristics, root yield and the levels of the efficacious constitutents saikosaponins in Bupleurum falcatum L. in the field of Chonnam RDA. Naju, 1994. Five organic fertilizers were used ; rice straw manure(RSM), rice straw(byproduct of mushroom)manure[RS(BM)M], pig-dropping sawdust manure(PSM),poultry manure(PM)and sawdust(byproduct of mushroom) manure[S(BM)M]. The results were summarized as follows ; The chemical properties, such as organic matter, available phosphate, $K_2O$, CaO, and MgO of soil were increased on all plots with the addition of organic fertilizers, specifically in PM+RS(BM)M application. PM+RS(BM)M treatment was higher in emergence rate and better in the growth charaters of shoot, root parts of plants than those of conventional fertilization and the highest dried root yield resulted from using 39% PM+RS(BM)M treatment. Thus, the organic constituents of the plant leaves were affected in the same ways by all treatments, but the T-N, $P_2O_5$, $K_2O$, CaO and MgO contents of the roots were highly increased in PM+RS(BM)M treatment, also the total saikosaponin levels in the roots increased with different organic fertilizers in the following order : 1.70% in PM+RS(BM) treatment, 1.66% in RS(BM) treatment, and 1.57% in RSM+N-P-K treatment. There were significant positive correlation observed between the growth characters of shoot, root parts of plants, yield and contents of T-N, $P_2O_5$, $K_2O$, total-saikosaponin in B. falcatum roots.

• Journal of Mushroom
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• v.18 no.4
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• pp.331-338
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• 2020

This study was conducted to cultivate new Lepista nuda varieties with shorter cultivation period and better fruiting body compared to that of wild strains, for mass production and commercial application. Eighteen genetic resources of L. nuda were collected and grown in boxes using rice straw-fermented growth medium. Four lines with fruiting bodies were formed and selected as cross-breeding lines. Although 657 combinations were crossed through monospore crossing, only 17 combinations were bred between the 'CBMLN-19' line and the 'CBMLN-30' line. Among them, 8 lines with fast mycelial growth and high density were selected. After inoculating the rice straw-fermented growth medium with 14 genetic resources and 8 cross-breeding lines, their incubation period was investigated. Six of the cross-breeding lines completed their incubation in 20 days, while 7 of the 14 genetic resources took more than 40 days to complete their incubation, reducing the incubation period by more than 20 days in most cross-breeding lines. After the incubations were completed, the clay loam soil was covered with for post-cultivation, and when the mycelial cultivation was complete, the formation of fruiting bodies was induced after scraping the mycelial bodies under these environmental conditions: 14℃, 95% relative humidity or higher, and 1,500 to 2,000 ppm CO2 concentration. The temperature was reduced to 6℃ at night, resulting in a low temperature shock. Thus, 4 lines of fruiting bodies occurred from two genetic resources 'CBMLN-31' and 'CBMLN-44' and two cross-bred lines 'CBMLN-96' and 'CBMLN-103'. After inoculation, the longest period for fruiting bodies to occur was 100 days for the control:, the genetic resource 'CBMLN-31', and the shortest period (45 days) was observed for the cross-breeding line 'CBMLN-103'. The result of the investigation of the fruiting body characteristics shows that the cross-bred line 'CBMLN-103' showed a small form with 1.9 g of individual weight and 123validstipes per box, which was the highest incidence among the four lines. Another cross-bred line, 'CBMLN-96', had an individual weight of 5.5 g, which is larger than that of 'CBMLN-103'; however, the number of valid stipes per box was 30 less than that of 'CBMLN-103'. Quantity analysis showed that the control, 'CBMLN-31', had the highest quantity of 783 g per box, followed by the cross-bred line, 'CBMLN-96' with 165 g per box, and then the 'CBMLN-103' with 232 g. The quantity of the two crossbred lines was lower than that of the control 'CBMLN-31'; however, the amount of fruiting bodies was higher, and the cultivation period was shortened by 32 to 33 days. Therefore, these two lines would be selected as superior lines.

• The Journal of the Korean Society for Microbiology
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• v.21 no.3
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• pp.363-373
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• 1986

We developed a giant colony test system with rapidly growing mycobacteria by stab-culture with a loopful inoculum of cells into Middlebrook 7H10 agar medium containing soluble extracts of the fruits of Gardenia jasminoides(7H10-crocin agar medium) and assessed the significance of the giant colony test with 28 strains of 10 clusters of rapidly growing mycobacteria classified by the simple biological 5-test characters. Of the 10 clusters of mycobacteria tested, some of strains which belonged to cluster No. 1a, 5a and 11a did grow as gravis types, whereas most of other clusters gave mitis or intermedius types in their colonial sizes at 12 days culture. By this test, pathogenic strains of M. fortuitum-chelonei complex which belonged to cluster No. 5a, b, 7a and 8a, b could be divided into gravis, intermedius and mitis colony types and the gravis ones were characterized by bluish-white "mushroom-shaped" colonies with central complexes in the texture, whereas the intermedius gave grayish-white "flower-shaped" colonies with radiated folds, but without any central complexes. The mitis colonies were characterized by grayish-white smooth or smooth mucoid colonies and were common among the clusters in their shapes. The colony of M. chelonei was bluish-white mitis type and was characterized by its hilly rhizoid colony. The gravis colony of cluster No. 1a identified as M. phlei was characterized by yellow "round straw- mat-shaped" or "chrysanthemum-shaped" colony with whole complexes in the texture, and the gravis colonies of the cluster No. 11a gave grayish-white "flower-shaped" colonies with central stamens, radiating trough and fine cup-shaped strands in the texture. The four colony types of pathogenic species of M. fortuitum-chelonei complex on 7H10-crocin agar medium were distinctive from those of other clusters of rapidly growing mycobacteria and these results indicated that the giant colony test, in conjunction with the simple 5-test characters, would be of value in the differentiation of M. fortuitum complex from other clusters of rapidly growing mycobacteria.

• Korean journal of applied entomology
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• v.57 no.4
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• pp.355-359
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• 2018

We used the entomopathogenic nematode, Steinernema carpocapsae, for the effective biological control of Lycoriella mali during Agaricus bisporus cultivation. The concentrations of two S. carpocapsae products distributed in Korea were $6{\times}10^4$ and $2.5{\times}10^5m^{-2}$ respectively. As a result of investigating the density of A. bisporus larvae in a rice straw medium and soil in an A. bisporus cultivation house, the mean numbers per $30cm^2$ were 0.8 and 22.2, respectively, and A. bisporus larvae were detected mainly in the soil. The control effects after once spraying S. carpocapsae at $6{\times}10^4$ and $2.5{\times}10^5m^{-2}$ was higher at the 14th day than at the 7th day. Therefore, we investigated the control effect after twice spraying of $6{\times}10^4$ and $2.5{\times}10^5m^{-2}$ at the 14th day. The control effects after this spraying were 51.9% and 96.8%, respectively, and the control effect of spraying at $2.5{\times}10^5m^{-2}$ was better than at $6{\times}10^4m^{-2}$. The control effect of diflubenzuron WP was lower than 50%.